FastQCFastQC Report
Sat 18 Jun 2016
SRR3552759_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552759_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489671
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC44680.9124493792771065No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC38410.7844042224268948No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT38270.781545159913493Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC21990.44907703335504856No Hit
ACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC14100.2879484388497583No Hit
GCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG8790.17950828209144506No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG8710.17787453208378687No Hit
ACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG8230.16807203203783766No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC7930.16194546950911937No Hit
AGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG6130.1251860943368098No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5790.11824265680426245No Hit
GGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG5210.10639796924874047No Hit
GGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT4930.10067984422193677No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.10006718796906494No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACGG351.21001E-745.0000042
GACCTAG351.21001E-745.00000410
TGGTACG351.21001E-745.0000041
TTTAGCG207.0293155E-445.01
CTTCGAC207.0293155E-445.021
AACGTAA207.0293155E-445.020
CGTGGGT253.8875147E-545.04
TCGTTGA253.8875147E-545.024
TACCATC253.8875147E-545.039
GGCGTTG207.0293155E-445.01
CGTTGAT253.8875147E-545.025
TAGCGCG207.0293155E-445.01
ATAGCGG502.1827873E-1145.02
GATCATA207.0293155E-445.08
GCTTGCG207.0293155E-445.01
CGTTCAT207.0293155E-445.010
TCGGCCG207.0293155E-445.011
AGTATAG253.8875147E-545.01
AACGGTT207.0293155E-445.016
AAACGTA207.0293155E-445.019