##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552759_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	489671
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53639076032683	31.0	31.0	34.0	30.0	34.0
2	31.694635786068606	31.0	31.0	34.0	30.0	34.0
3	31.737299125331088	31.0	31.0	34.0	30.0	34.0
4	35.390458083080276	37.0	35.0	37.0	33.0	37.0
5	35.27670211223454	37.0	35.0	37.0	33.0	37.0
6	35.253864329314986	37.0	35.0	37.0	32.0	37.0
7	35.70628646581072	37.0	35.0	37.0	35.0	37.0
8	35.68326080164029	37.0	35.0	37.0	35.0	37.0
9	37.27144347939739	39.0	37.0	39.0	34.0	39.0
10	36.71724688617459	39.0	37.0	39.0	32.0	39.0
11	36.55240967915192	38.0	35.0	39.0	32.0	39.0
12	36.40061592375289	38.0	35.0	39.0	32.0	39.0
13	36.27327532159348	38.0	35.0	39.0	32.0	39.0
14	37.26131831372493	39.0	36.0	40.0	32.0	41.0
15	37.37932407677808	39.0	36.0	40.0	32.0	41.0
16	37.4615037443508	39.0	36.0	40.0	32.0	41.0
17	37.459402333403446	39.0	36.0	41.0	32.0	41.0
18	37.44230922394832	39.0	36.0	40.0	32.0	41.0
19	37.44247055676158	39.0	36.0	40.0	32.0	41.0
20	37.38228524866696	39.0	35.0	40.0	32.0	41.0
21	37.328150942163205	39.0	35.0	40.0	32.0	41.0
22	37.271711005961144	39.0	35.0	40.0	32.0	41.0
23	37.23988964018698	39.0	35.0	40.0	32.0	41.0
24	37.22620494168534	39.0	35.0	40.0	32.0	41.0
25	37.18668452900008	39.0	35.0	40.0	32.0	41.0
26	37.06723085500264	39.0	35.0	40.0	32.0	41.0
27	37.00333897657815	39.0	35.0	40.0	31.0	41.0
28	36.95304398259239	39.0	35.0	40.0	31.0	41.0
29	36.897345360456306	39.0	35.0	40.0	31.0	41.0
30	36.76009606450045	39.0	35.0	40.0	31.0	41.0
31	36.66383551404923	39.0	35.0	40.0	31.0	41.0
32	36.52077211025362	38.0	35.0	40.0	30.0	41.0
33	36.39715033154914	38.0	35.0	40.0	30.0	41.0
34	36.34180909222723	38.0	35.0	40.0	30.0	41.0
35	36.303097385795766	38.0	35.0	40.0	30.0	41.0
36	36.16054248669004	38.0	35.0	40.0	30.0	41.0
37	36.1094183645754	38.0	35.0	40.0	30.0	41.0
38	36.05883950652581	38.0	35.0	40.0	30.0	41.0
39	36.02742453606605	38.0	35.0	40.0	30.0	41.0
40	35.938944720026306	38.0	35.0	40.0	29.0	41.0
41	35.852145624306935	38.0	35.0	40.0	29.0	41.0
42	35.72596498465296	38.0	34.0	40.0	29.0	41.0
43	35.60201032938442	38.0	34.0	40.0	28.0	41.0
44	35.445476656775675	38.0	34.0	40.0	27.0	41.0
45	35.39425246747306	38.0	34.0	40.0	27.0	41.0
46	35.239450569872425	38.0	34.0	40.0	27.0	41.0
47	35.10993912239034	37.0	34.0	40.0	26.0	41.0
48	35.076949625360704	37.0	34.0	40.0	26.0	41.0
49	35.020211529782244	37.0	34.0	40.0	26.0	41.0
50	34.892431857308274	37.0	33.0	40.0	26.0	41.0
51	33.191937035274705	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	7.0
12	1.0
13	3.0
14	3.0
15	19.0
16	33.0
17	83.0
18	169.0
19	308.0
20	508.0
21	825.0
22	1208.0
23	1745.0
24	2603.0
25	3390.0
26	4415.0
27	5527.0
28	6951.0
29	8804.0
30	11336.0
31	14473.0
32	18729.0
33	25209.0
34	38310.0
35	45118.0
36	46419.0
37	63511.0
38	95888.0
39	94058.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.19185738996183	20.391242283083947	25.294738712319088	11.122161614635132
2	31.279981865374918	23.651594642116848	25.558180901053973	19.510242591454265
3	31.16949952110703	23.696931204829365	28.563668258892193	16.56990101517141
4	29.526151232153836	25.973970278002984	26.120190903688396	18.379687586154787
5	24.305707301433003	29.207161543158573	25.75239293321434	20.734738222194085
6	25.498344807023493	33.96831750297649	25.29902730608919	15.23431038391083
7	76.60776317159889	6.376117842388053	10.607734580973755	6.408384405039302
8	74.79430066309828	5.842085808634778	11.179955521156042	8.183658007110898
9	69.43703016923608	7.761129411380294	14.284080535706629	8.517759883677
10	38.079036740995484	23.541316516599924	21.740515570658665	16.639131171745927
11	28.267551070004142	26.09507199732065	25.37867261896253	20.258704313712677
12	25.07806261755342	23.990801987456884	32.11401124428443	18.81712415070527
13	22.535947605637254	27.13474965844414	32.01006390004718	18.31923883587143
14	18.030473522017846	30.949555926326045	29.065025292492308	21.954945259163804
15	16.688143671975673	26.695475125135037	36.34848704538353	20.267894157505754
16	17.45130914430301	27.14271418973147	31.535459522822467	23.87051714314305
17	17.280173831000813	26.750409969142545	29.196337949357837	26.773078250498806
18	18.94210602629112	25.680916370379297	32.475478433478806	22.901499169850776
19	20.147813531942877	26.898060126084655	29.56679076359433	23.387335578378135
20	21.548958382260743	27.412487159746036	30.564807799522537	20.473746658470688
21	21.28306556851437	28.194032319659527	30.155961860106075	20.366940251720035
22	18.165462116400604	27.286075752903482	27.006908720344885	27.54155341035103
23	18.170771803925494	27.520927316504345	28.56489357139794	25.743407308172223
24	20.72228087838569	24.842802616450637	30.019339515715654	24.415576989448017
25	17.70106867672376	28.81465310381869	27.117186845861813	26.36709137359574
26	17.670640082831127	31.613471085688143	28.139914350655847	22.575974480824883
27	19.813098999123902	30.087344359784428	28.485044039773644	21.61451260131803
28	16.21558148226054	28.02820669388222	31.272017334087582	24.484194489769664
29	20.582595252730915	27.490090285109797	28.246924976157462	23.680389486001825
30	23.01831229539834	28.890009822921918	26.41732918633123	21.67434869534851
31	21.290417443548833	29.134051230315865	24.880583085377733	24.69494824075757
32	24.36431808295774	30.358955298557603	23.092852139497744	22.183874478986912
33	23.317901203052664	29.73935560815323	23.76473183014718	23.178011358646927
34	20.211734000992504	25.923936684018457	28.114999663039058	25.74932965194998
35	23.74308464254571	27.481308878818634	23.911973549587376	24.86363292904828
36	22.33315838593668	31.588760616822313	25.50937261957518	20.56870837766582
37	21.641265257693433	27.569735597983136	29.44466795052188	21.34433119380155
38	24.034709018912697	27.598326223117155	26.19534340404067	22.171621353929474
39	25.56287793232599	24.489095739792635	27.243189815202452	22.704836512678924
40	25.313118399905242	23.373040265811127	27.478654035056188	23.83518729922744
41	20.935893691887	27.528483410289766	24.78684667868834	26.74877621913489
42	22.329074010917534	27.438014503615694	27.08083590819142	23.152075577275355
43	24.283039020076746	25.20488246189789	29.090552636361966	21.421525881663403
44	22.461816199039763	24.941848710664914	27.458436378711422	25.1378987115839
45	23.838454799242754	22.25208354180664	26.327064498408113	27.582397160542488
46	25.223262149484043	24.096587300452754	27.045914501777723	23.63423604828548
47	19.481039310067374	25.157095274173884	31.377598428332494	23.98426698742625
48	20.918126660553717	23.721845892446154	29.474075450659733	25.8859519963404
49	22.828184638257117	22.0993279160906	31.908771399572366	23.16371604607992
50	22.263928229362165	20.94732994194061	30.2929926419984	26.495749186698824
51	20.7155416596041	20.483957596018552	26.75102262539542	32.04947811898193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	468.0
1	511.0
2	554.0
3	846.5
4	1139.0
5	892.5
6	646.0
7	654.0
8	662.0
9	724.0
10	786.0
11	862.0
12	938.0
13	979.5
14	1021.0
15	1067.5
16	1114.0
17	1218.0
18	1322.0
19	1304.0
20	1286.0
21	1582.5
22	1879.0
23	1921.0
24	1963.0
25	2417.5
26	3205.5
27	3539.0
28	4274.0
29	5009.0
30	6191.5
31	7374.0
32	8102.5
33	8831.0
34	9684.0
35	10537.0
36	11588.5
37	12640.0
38	13668.0
39	14696.0
40	17376.0
41	20056.0
42	23724.0
43	27392.0
44	32206.0
45	37020.0
46	43843.5
47	50667.0
48	61373.0
49	72079.0
50	69765.0
51	67451.0
52	54907.0
53	42363.0
54	34161.0
55	25959.0
56	21800.0
57	17641.0
58	15386.5
59	13132.0
60	11996.5
61	10861.0
62	9977.0
63	9093.0
64	7404.5
65	5716.0
66	4741.5
67	3767.0
68	3107.0
69	2447.0
70	2129.0
71	1811.0
72	1450.0
73	1089.0
74	873.5
75	571.5
76	485.0
77	416.0
78	347.0
79	292.0
80	237.0
81	151.5
82	66.0
83	49.5
84	33.0
85	23.5
86	14.0
87	8.0
88	2.0
89	1.0
90	0.0
91	1.0
92	2.0
93	4.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	489671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.28916231051876
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.83023217569033	19.939425873086186
2	13.879315385050203	8.407856729133314
3	6.08339157337585	5.527825042932696
4	3.1636501435091953	3.832972507617839
5	1.874094970196181	2.8382383368799475
6	1.273559845719693	2.314503652749781
7	0.8539136074579323	1.810502949881774
8	0.6316601539948089	1.5305965543548832
9	0.487066448685666	1.3277551248223285
>10	5.511397128128322	37.46074553000497
>50	0.3278875296766208	6.122066391808065
>100	0.0757183573686217	4.623340635991655
>500	0.004732397335538856	1.0400366325716013
>1k	0.0033802838110991835	3.2241340381649666
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	4468	0.9124493792771065	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	3841	0.7844042224268948	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	3827	0.781545159913493	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2199	0.44907703335504856	No Hit
ACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1410	0.2879484388497583	No Hit
GCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	879	0.17950828209144506	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	871	0.17787453208378687	No Hit
ACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	823	0.16807203203783766	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	793	0.16194546950911937	No Hit
AGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	613	0.1251860943368098	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	579	0.11824265680426245	No Hit
GGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	521	0.10639796924874047	No Hit
GGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	493	0.10067984422193677	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	490	0.10006718796906494	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.846895160219821	0.0
2	0.0	0.0	0.0	3.500922047660572	0.0
3	0.0	0.0	0.0	4.690700490737658	0.0
4	2.0421875095727538E-4	0.0	0.0	5.928674559040662	0.0
5	2.0421875095727538E-4	0.0	0.0	8.922317229323362	0.0
6	2.0421875095727538E-4	0.0	0.0	10.33530676719675	0.0
7	2.0421875095727538E-4	0.0	0.0	11.910037555828302	0.0
8	2.0421875095727538E-4	0.0	0.0	14.406611786280992	0.0
9	2.0421875095727538E-4	0.0	0.0	15.175291164884177	0.0
10	2.0421875095727538E-4	0.0	0.0	17.32387664370567	0.0
11	2.0421875095727538E-4	0.0	0.0	20.528477283727238	0.0
12	2.0421875095727538E-4	0.0	0.0	22.81756526320734	0.0
13	2.0421875095727538E-4	0.0	0.0	23.750232298829214	0.0
14	2.0421875095727538E-4	0.0	0.0	24.107819331755405	0.0
15	2.0421875095727538E-4	0.0	0.0	24.798078709990993	0.0
16	2.0421875095727538E-4	0.0	0.0	26.320121060875568	0.0
17	2.0421875095727538E-4	0.0	0.0	28.089676537920358	0.0
18	4.0843750191455077E-4	0.0	0.0	30.125124828711524	0.0
19	4.0843750191455077E-4	0.0	0.0	31.22116686509922	0.0
20	4.0843750191455077E-4	0.0	0.0	32.20264218219989	0.0
21	0.0014295312567009278	0.0	0.0	33.383230781483896	0.0
22	0.0028590625134018556	0.0	0.0	34.59629016217011	0.0
23	0.0028590625134018556	0.0	0.0	35.79689219904793	0.0
24	0.0038801562681882325	0.0	0.0	36.79572610997997	0.0
25	0.006943437532547363	0.0	0.0	37.6458887702151	0.0
26	0.00796453128733374	0.0	0.0	38.35799955480312	0.0
27	0.00796453128733374	0.0	0.0	39.04825893303872	0.0
28	0.00796453128733374	0.0	0.0	39.788551905258835	0.0
29	0.00796453128733374	0.0	0.0	40.51557065866674	0.0
30	0.00796453128733374	0.0	0.0	41.34081863128509	0.0
31	0.00796453128733374	0.0	0.0	42.08029472850138	0.0
32	0.00796453128733374	0.0	0.0	42.80302488813918	0.0
33	0.00796453128733374	0.0	0.0	43.496143328888174	0.0
34	0.009394062544034668	0.0	0.0	44.168227238288566	0.0
35	0.009394062544034668	0.0	0.0	44.87992958537467	0.0
36	0.01061937504977832	0.0	0.0	45.58877286994737	0.0
37	0.01061937504977832	0.0	0.0	46.2267522479379	0.0
38	0.01061937504977832	0.0	0.0	46.86187256341503	0.0
39	0.01061937504977832	0.0	0.0	47.425516316057106	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCACGG	35	1.21001E-7	45.000004	2
GACCTAG	35	1.21001E-7	45.000004	10
TGGTACG	35	1.21001E-7	45.000004	1
TTTAGCG	20	7.0293155E-4	45.0	1
CTTCGAC	20	7.0293155E-4	45.0	21
AACGTAA	20	7.0293155E-4	45.0	20
CGTGGGT	25	3.8875147E-5	45.0	4
TCGTTGA	25	3.8875147E-5	45.0	24
TACCATC	25	3.8875147E-5	45.0	39
GGCGTTG	20	7.0293155E-4	45.0	1
CGTTGAT	25	3.8875147E-5	45.0	25
TAGCGCG	20	7.0293155E-4	45.0	1
ATAGCGG	50	2.1827873E-11	45.0	2
GATCATA	20	7.0293155E-4	45.0	8
GCTTGCG	20	7.0293155E-4	45.0	1
CGTTCAT	20	7.0293155E-4	45.0	10
TCGGCCG	20	7.0293155E-4	45.0	11
AGTATAG	25	3.8875147E-5	45.0	1
AACGGTT	20	7.0293155E-4	45.0	16
AAACGTA	20	7.0293155E-4	45.0	19
>>END_MODULE