FastQCFastQC Report
Sat 18 Jun 2016
SRR3552756_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552756_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721786
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC41570.5759324786016908TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC38010.5266103803620464TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT33820.4685599332766221TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC15360.21280545757329733TruSeq Adapter, Index 20 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA10990.15226119653193607No Hit
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC10230.14173175982909061TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG8270.11457689675333131TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7710.10681836444597154No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC7440.10307764351206591No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7270.10072237477590311No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCTA253.889357E-545.0000041
CACGAGG253.889357E-545.0000042
CCCGTCG253.889357E-545.00000425
CGAATAT253.889357E-545.00000414
TTAACGG253.889357E-545.0000041
TGCGTTA253.889357E-545.00000430
CTATGCG207.031541E-445.01
TCGCAAG207.031541E-445.01
CGTTCTA207.031541E-445.024
TCGATAG207.031541E-445.01
ACCTAGT207.031541E-445.011
ACAGCGT207.031541E-445.045
TAGGCGA207.031541E-445.01
ACGTAAT207.031541E-445.029
TATGTTA406.8084773E-945.041
CGATAAT207.031541E-445.010
AATCGTA207.031541E-445.017
TACGTAG207.031541E-445.01
TCTATAC207.031541E-445.041
TTGTGCG1000.040.5000041