Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552756_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 721786 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC | 4157 | 0.5759324786016908 | TruSeq Adapter, Index 20 (95% over 23bp) |
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC | 3801 | 0.5266103803620464 | TruSeq Adapter, Index 20 (95% over 23bp) |
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT | 3382 | 0.4685599332766221 | TruSeq Adapter, Index 22 (95% over 22bp) |
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC | 1536 | 0.21280545757329733 | TruSeq Adapter, Index 20 (95% over 23bp) |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 1099 | 0.15226119653193607 | No Hit |
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC | 1023 | 0.14173175982909061 | TruSeq Adapter, Index 22 (95% over 21bp) |
GCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG | 827 | 0.11457689675333131 | TruSeq Adapter, Index 20 (95% over 22bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 771 | 0.10681836444597154 | No Hit |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC | 744 | 0.10307764351206591 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC | 727 | 0.10072237477590311 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACGCTA | 25 | 3.889357E-5 | 45.000004 | 1 |
CACGAGG | 25 | 3.889357E-5 | 45.000004 | 2 |
CCCGTCG | 25 | 3.889357E-5 | 45.000004 | 25 |
CGAATAT | 25 | 3.889357E-5 | 45.000004 | 14 |
TTAACGG | 25 | 3.889357E-5 | 45.000004 | 1 |
TGCGTTA | 25 | 3.889357E-5 | 45.000004 | 30 |
CTATGCG | 20 | 7.031541E-4 | 45.0 | 1 |
TCGCAAG | 20 | 7.031541E-4 | 45.0 | 1 |
CGTTCTA | 20 | 7.031541E-4 | 45.0 | 24 |
TCGATAG | 20 | 7.031541E-4 | 45.0 | 1 |
ACCTAGT | 20 | 7.031541E-4 | 45.0 | 11 |
ACAGCGT | 20 | 7.031541E-4 | 45.0 | 45 |
TAGGCGA | 20 | 7.031541E-4 | 45.0 | 1 |
ACGTAAT | 20 | 7.031541E-4 | 45.0 | 29 |
TATGTTA | 40 | 6.8084773E-9 | 45.0 | 41 |
CGATAAT | 20 | 7.031541E-4 | 45.0 | 10 |
AATCGTA | 20 | 7.031541E-4 | 45.0 | 17 |
TACGTAG | 20 | 7.031541E-4 | 45.0 | 1 |
TCTATAC | 20 | 7.031541E-4 | 45.0 | 41 |
TTGTGCG | 100 | 0.0 | 40.500004 | 1 |