##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552756_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	721786
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.386063182162026	33.0	33.0	33.0	27.0	33.0
2	31.56981986350525	33.0	33.0	33.0	27.0	33.0
3	31.6797804335357	33.0	33.0	33.0	27.0	33.0
4	31.605924747778428	33.0	33.0	33.0	27.0	33.0
5	31.6934700867016	33.0	33.0	33.0	27.0	33.0
6	35.06903431210913	37.0	37.0	37.0	33.0	37.0
7	35.38433690872364	37.0	37.0	37.0	33.0	37.0
8	35.45756498463533	37.0	37.0	37.0	33.0	37.0
9	35.33089031929131	37.0	37.0	37.0	33.0	37.0
10	35.369461308476474	37.0	37.0	37.0	33.0	37.0
11	35.356317523476484	37.0	37.0	37.0	33.0	37.0
12	35.35926299484889	37.0	37.0	37.0	33.0	37.0
13	35.39371364919796	37.0	37.0	37.0	33.0	37.0
14	35.466035639372336	37.0	37.0	37.0	33.0	37.0
15	35.51799009678769	37.0	37.0	37.0	33.0	37.0
16	35.5263942498192	37.0	37.0	37.0	33.0	37.0
17	35.53784085587695	37.0	37.0	37.0	33.0	37.0
18	35.53397544424525	37.0	37.0	37.0	33.0	37.0
19	35.51157822401654	37.0	37.0	37.0	33.0	37.0
20	35.49065789583062	37.0	37.0	37.0	33.0	37.0
21	35.4120417963219	37.0	37.0	37.0	33.0	37.0
22	35.45657022995736	37.0	37.0	37.0	33.0	37.0
23	35.473468590413226	37.0	37.0	37.0	33.0	37.0
24	35.478227618712474	37.0	37.0	37.0	33.0	37.0
25	35.47393410235167	37.0	37.0	37.0	33.0	37.0
26	35.41525604542066	37.0	37.0	37.0	33.0	37.0
27	35.41494016231958	37.0	37.0	37.0	33.0	37.0
28	35.37928555001067	37.0	37.0	37.0	33.0	37.0
29	35.39086238857501	37.0	37.0	37.0	33.0	37.0
30	35.34522559318136	37.0	37.0	37.0	33.0	37.0
31	35.34187418431502	37.0	37.0	37.0	33.0	37.0
32	35.32337008476197	37.0	37.0	37.0	33.0	37.0
33	35.3065326842028	37.0	37.0	37.0	33.0	37.0
34	35.30052813437778	37.0	37.0	37.0	33.0	37.0
35	35.27173428135209	37.0	37.0	37.0	33.0	37.0
36	35.24626412814879	37.0	37.0	37.0	33.0	37.0
37	35.254073922187466	37.0	37.0	37.0	33.0	37.0
38	35.217730740136275	37.0	37.0	37.0	33.0	37.0
39	35.234975463641575	37.0	37.0	37.0	33.0	37.0
40	35.21960110060323	37.0	37.0	37.0	33.0	37.0
41	35.20769729532022	37.0	37.0	37.0	33.0	37.0
42	35.1921178853566	37.0	37.0	37.0	33.0	37.0
43	35.17713976164681	37.0	37.0	37.0	33.0	37.0
44	35.11942597944543	37.0	37.0	37.0	33.0	37.0
45	35.095802634021716	37.0	37.0	37.0	33.0	37.0
46	35.097902979553496	37.0	37.0	37.0	33.0	37.0
47	35.08688863458144	37.0	37.0	37.0	33.0	37.0
48	35.07754098860327	37.0	37.0	37.0	33.0	37.0
49	35.0581280324085	37.0	37.0	37.0	33.0	37.0
50	35.068164248128944	37.0	37.0	37.0	33.0	37.0
51	34.6527724283929	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	509.0
15	2004.0
16	1967.0
17	1624.0
18	1598.0
19	1627.0
20	1687.0
21	1883.0
22	2155.0
23	2604.0
24	3153.0
25	4196.0
26	5399.0
27	7212.0
28	9030.0
29	10886.0
30	12816.0
31	15772.0
32	20349.0
33	27658.0
34	41617.0
35	84766.0
36	461274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.289027495684316	18.80848340089722	23.27185620114549	13.630632902272973
2	29.610161460599127	23.556566627781642	26.01615991443447	20.81711199718476
3	32.33021976042761	23.94477033358918	25.149836655185887	18.575173250797327
4	29.05708894326019	26.267342397885248	24.98621475063246	19.689353908222103
5	25.30431457523421	30.14397619238943	24.244859279620275	20.30684995275608
6	26.85241886099204	34.83968932619918	22.748570906058028	15.559320906750754
7	79.75757357443895	7.012327753655516	7.97826502592181	5.251833645983712
8	80.34126458534801	5.586420351738604	7.825449648510778	6.246865414402607
9	74.88452255931813	7.756177038623637	10.737531622946413	6.6217687791118145
10	40.98430836840837	25.839237668782715	17.356668042882518	15.819785919926405
11	31.120165810919026	25.837852216584974	22.454162314037678	20.587819658458322
12	26.993457894722255	23.55421135904548	29.478404956593785	19.97392578963848
13	23.709936186071772	26.322621940575186	29.94128453585966	20.026157337493384
14	21.03351963047219	28.590053007401085	28.24161177966877	22.13481558245796
15	19.886919391620232	25.67145940763606	33.107319898141554	21.334301302602157
16	21.453173101168492	25.473616833798385	30.074703582502295	22.998506482530836
17	21.21529095881605	25.645412906318494	27.865738598421142	25.27355753644432
18	23.26285076186016	24.071539209682648	30.193436835848853	22.472173192608334
19	23.169748374171846	26.99124117120587	27.31003372190649	22.52897673271579
20	25.640979459285717	25.877337604220642	27.51826718722724	20.963415749266403
21	23.908748576447866	27.295209383390645	27.441651680692058	21.354390359469427
22	21.872272390985696	25.46017794748028	26.535150307708932	26.132399353825097
23	21.55347984028507	26.697248214844844	26.72135508308557	25.027916861784515
24	22.21572599080614	24.89699162909782	28.927964798430562	23.95931758166548
25	21.049175240306685	26.97184484043747	26.18532362777887	25.793656291476974
26	20.77083789378015	28.357712673839615	27.414635362836076	23.45681406954416
27	21.98560238076106	27.015348039446597	26.675219524900733	24.323830054891616
28	18.334381658829628	28.69590155530864	27.930716306495277	25.039000479366457
29	20.89954640295046	26.203611596789077	26.74282959215058	26.154012408109885
30	21.371847057160988	26.539999390401032	26.21275558129418	25.875397971143805
31	22.260337551573457	28.891527405629922	24.455586558896957	24.39254848389966
32	22.142851205204867	27.84758917463071	24.79349834992643	25.216061270237994
33	22.503900047936646	26.48291875985403	24.403632101481605	26.609549090727725
34	19.48209025943978	26.622156705727186	28.292734965765476	25.603018069067563
35	21.144355806291614	25.49522988808317	26.022394449324317	27.3380198563009
36	21.97271767532205	28.58021629679711	25.029717949641583	24.417348078239257
37	21.245355271507066	27.76584749496388	27.181602303175733	23.80719493035332
38	19.620219843554736	28.747856012723993	25.614101686649505	26.017822457071766
39	22.716151324630847	24.185284835117336	27.142394005979614	25.95616983427221
40	22.117774520425723	23.64578974931628	29.734713613176204	24.501722117081794
41	19.64169435261975	25.7450269193362	28.557910516413454	26.055368211630594
42	21.900119980160323	25.084858947111748	27.40091938607842	25.614101686649505
43	23.369530581086362	23.323672113341072	27.541958419808644	25.764838885763925
44	20.707245637903757	24.87205348953845	26.136971346077647	28.28372952648015
45	21.366859429249114	23.137328792744665	26.93817835203232	28.557633425973904
46	21.389026664412995	25.250004849082693	28.16956826538614	25.191400221118172
47	19.42805762372781	25.068094975519063	30.446281861936917	25.057565538816213
48	19.997755567439658	24.032608002926075	29.2682318581962	26.70140457143807
49	22.309521104593326	23.123197180327686	29.594644395984403	24.972637319094577
50	20.513697965879082	22.84541401468025	28.977702532329523	27.663185487111136
51	19.901051004037207	22.455963401894742	26.76305719423763	30.87992839983042
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	277.0
1	362.5
2	448.0
3	978.0
4	1508.0
5	1185.5
6	863.0
7	854.0
8	845.0
9	912.0
10	979.0
11	1019.0
12	1059.0
13	1216.5
14	1374.0
15	1410.5
16	1447.0
17	1446.0
18	1445.0
19	1489.0
20	1533.0
21	1835.0
22	2137.0
23	2336.0
24	2535.0
25	3436.0
26	5008.5
27	5680.0
28	6708.0
29	7736.0
30	9256.5
31	10777.0
32	11936.5
33	13096.0
34	14779.0
35	16462.0
36	18127.0
37	19792.0
38	20761.0
39	21730.0
40	24547.5
41	27365.0
42	31382.0
43	35399.0
44	40628.0
45	45857.0
46	53440.5
47	61024.0
48	70064.0
49	79104.0
50	80179.0
51	81254.0
52	69639.5
53	58025.0
54	51202.5
55	44380.0
56	39553.5
57	34727.0
58	32225.5
59	29724.0
60	28824.5
61	27925.0
62	26147.5
63	24370.0
64	21147.0
65	17924.0
66	15360.5
67	12797.0
68	10762.0
69	8727.0
70	7549.5
71	6372.0
72	5114.5
73	3857.0
74	3499.0
75	2366.0
76	1591.0
77	1379.5
78	1168.0
79	914.5
80	661.0
81	446.0
82	231.0
83	151.5
84	72.0
85	47.0
86	22.0
87	13.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	721786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.429904574142572
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.11178344382569	19.648414082567708
2	12.481222507491282	7.0967992971323515
3	5.137356299288707	4.3816364805644445
4	2.6396404596243506	3.0017890550866446
5	1.4915623429477105	2.120248753819396
6	0.9709080104934535	1.6561693253159697
7	0.6609884068892872	1.3154286132733868
8	0.4842261148121711	1.1013201789134264
9	0.3834014409646375	0.9810059742192061
>10	6.032449109613255	43.27820449205514
>50	0.5370681616373693	9.37620814503802
>100	0.06255206977995936	3.2036686119199924
>500	0.00390950436124746	0.7553816836380154
>1k	0.002932128270935595	2.0837253064563095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	4157	0.5759324786016908	TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	3801	0.5266103803620464	TruSeq Adapter, Index 20 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGCT	3382	0.4685599332766221	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1536	0.21280545757329733	TruSeq Adapter, Index 20 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1099	0.15226119653193607	No Hit
ACTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1023	0.14173175982909061	TruSeq Adapter, Index 22 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTG	827	0.11457689675333131	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	771	0.10681836444597154	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCC	744	0.10307764351206591	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	727	0.10072237477590311	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4806133673969847	0.0
2	0.0	0.0	0.0	1.9825820949699773	0.0
3	1.3854521977428212E-4	0.0	0.0	2.60063232038305	0.0
4	1.3854521977428212E-4	0.0	0.0	3.2540115768385642	0.0
5	1.3854521977428212E-4	0.0	0.0	4.778424630014991	0.0
6	1.3854521977428212E-4	0.0	0.0	5.5099433904232	0.0
7	1.3854521977428212E-4	0.0	0.0	6.368508117364427	0.0
8	1.3854521977428212E-4	0.0	0.0	7.901372428947084	0.0
9	1.3854521977428212E-4	0.0	0.0	8.460956571615409	0.0
10	1.3854521977428212E-4	0.0	0.0	9.968467107979373	0.0
11	1.3854521977428212E-4	0.0	0.0	12.19530442541141	0.0
12	1.3854521977428212E-4	0.0	0.0	13.826674388253583	0.0
13	1.3854521977428212E-4	0.0	0.0	14.455669686028823	0.0
14	2.7709043954856423E-4	0.0	0.0	14.681498394260903	0.0
15	2.7709043954856423E-4	0.0	0.0	15.176936100173736	0.0
16	2.7709043954856423E-4	0.0	0.0	16.181250398317506	0.0
17	2.7709043954856423E-4	0.0	0.0	17.417627939583202	0.0
18	2.7709043954856423E-4	0.0	0.0	18.878171646443683	0.0
19	2.7709043954856423E-4	0.0	0.0	19.70029898058427	0.0
20	2.7709043954856423E-4	0.0	0.0	20.454539157035466	0.0
21	5.541808790971285E-4	0.0	0.0	21.40274264117065	0.0
22	0.0012469069779685391	0.0	0.0	22.334597789372474	0.0
23	0.0034636304943570532	0.0	0.0	23.284325270925176	0.0
24	0.004156356593228464	0.0	0.0	24.067521398309193	0.0
25	0.00457199225255131	0.0	0.0	24.732399908005974	0.0
26	0.004849082692099874	0.0	0.0	25.36056393446257	0.0
27	0.004987627911874157	0.0	0.0	25.967114906634375	0.0
28	0.004987627911874157	0.0	0.0	26.564106258641758	0.0
29	0.005264718351422721	0.0	0.0	27.157079799275685	0.0
30	0.005264718351422721	0.0	0.0	27.84218591105951	0.0
31	0.005264718351422721	0.0	0.0	28.49307135355909	0.0
32	0.005264718351422721	0.0	0.0	29.123313558312297	0.0
33	0.005264718351422721	0.0	0.0	29.696336587298727	0.0
34	0.005264718351422721	0.0	0.0	30.305242828206698	0.0
35	0.0060959896700684135	0.0	0.0	30.941719567849752	0.0
36	0.0063730801096169775	0.0	0.0	31.57154613694364	0.0
37	0.006788715768939824	0.0	0.0	32.13168446049106	0.0
38	0.006788715768939824	0.0	0.0	32.72410382024589	0.0
39	0.0069272609887141064	0.0	0.0	33.26498435824469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGCTA	25	3.889357E-5	45.000004	1
CACGAGG	25	3.889357E-5	45.000004	2
CCCGTCG	25	3.889357E-5	45.000004	25
CGAATAT	25	3.889357E-5	45.000004	14
TTAACGG	25	3.889357E-5	45.000004	1
TGCGTTA	25	3.889357E-5	45.000004	30
CTATGCG	20	7.031541E-4	45.0	1
TCGCAAG	20	7.031541E-4	45.0	1
CGTTCTA	20	7.031541E-4	45.0	24
TCGATAG	20	7.031541E-4	45.0	1
ACCTAGT	20	7.031541E-4	45.0	11
ACAGCGT	20	7.031541E-4	45.0	45
TAGGCGA	20	7.031541E-4	45.0	1
ACGTAAT	20	7.031541E-4	45.0	29
TATGTTA	40	6.8084773E-9	45.0	41
CGATAAT	20	7.031541E-4	45.0	10
AATCGTA	20	7.031541E-4	45.0	17
TACGTAG	20	7.031541E-4	45.0	1
TCTATAC	20	7.031541E-4	45.0	41
TTGTGCG	100	0.0	40.500004	1
>>END_MODULE