FastQCFastQC Report
Sat 18 Jun 2016
SRR3552755_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552755_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390722
Sequences flagged as poor quality0
Sequence length51
%GC26

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7634319.53895608642462No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186004.760417893028803No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC119523.0589524009397984No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107642.7548999032560233No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG101292.5923802601338033No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC91202.334140386259284No Hit
AAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC75911.9428135605366474No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC53771.376170269398703No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT52731.3495528790290794No Hit
GAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT43631.1166507132948746No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT40201.0288645123642897No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG39361.0073658509119015No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT37100.9495242141471429No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT35600.9111337472678784No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC33180.8491971273693317No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC32440.8302578303755611No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT32220.8246272285666023No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC30700.7857248887956143No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG24210.6196221354313297No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT24190.6191102625396062No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT22700.5809757321062035No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC21950.5617804986665711No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21400.5477039941441741No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT20780.5318359345007448No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGT19520.4995879423221625No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC19350.4952370227425126No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC19110.4890945480418302No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC18630.47680959864046557No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA16570.4240866907929423No Hit
GAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC15720.4023320928946924No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT15130.38723184258884835No Hit
AAAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC12430.3181290022061722No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT12290.3145458919641074No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10820.2769232344224282No Hit
AAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT10240.2620789205624459No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT9850.25209739917383717No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC9000.23034280127558726No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG5300.1356463163067347No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTAACATCGT5230.13385476118570236No Hit
GAAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC5110.1307835238353612No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA4620.11824263798813478No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG4550.11645108286710244No Hit
TGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT4540.11619514642124068No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTTAACA4390.11235609973331423No Hit
AAAAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGT4230.10826111659952602No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTAT4200.10749330726194072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC4040.1033983241281525No Hit
AGGGTAGGGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC3920.10032708677781134No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATGA600.045.00000418
TAGGGTC600.045.0000045
CGAGATA302.1618107E-645.00000419
TGAGGGA551.8189894E-1245.0000044
TCACGAA207.027566E-445.06
GTTGATC406.7957444E-945.016
TTGATCC351.2091732E-745.017
ATCCTGC351.2091732E-745.020
CGGGTTA207.027566E-445.023
TCGTTGA207.027566E-445.024
ACTGCTA207.027566E-445.029
GGAGCAA207.027566E-445.08
CATATGC351.2091732E-745.033
TTAATCG207.027566E-445.020
TGCCAGT351.2091732E-745.024
TATGGGA207.027566E-445.04
CGTTGAT207.027566E-445.025
TCTTAGA253.886063E-545.020
GTCTCAA406.7957444E-945.042
ATTAATC207.027566E-445.019
Produced by FastQC (version 0.11.5)