##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552755_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390722
Sequences flagged as poor quality	0
Sequence length	51
%GC	26
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.487218533893664	31.0	31.0	34.0	30.0	34.0
2	31.73455551517447	31.0	31.0	34.0	30.0	34.0
3	31.629219752151148	31.0	31.0	34.0	30.0	34.0
4	35.43218963866892	37.0	35.0	37.0	33.0	37.0
5	35.321028762137786	37.0	35.0	37.0	33.0	37.0
6	35.28374905943356	37.0	35.0	37.0	32.0	37.0
7	35.08651931552357	37.0	35.0	37.0	32.0	37.0
8	34.83095141814385	37.0	35.0	37.0	31.0	37.0
9	36.26355311449061	38.0	35.0	39.0	32.0	39.0
10	36.808068140519346	39.0	37.0	39.0	32.0	39.0
11	36.77941605540512	39.0	37.0	39.0	32.0	39.0
12	36.77339387083399	39.0	37.0	39.0	32.0	39.0
13	36.70832714820256	39.0	37.0	39.0	32.0	39.0
14	37.810113072721784	40.0	37.0	41.0	32.0	41.0
15	37.66367391649305	40.0	37.0	41.0	32.0	41.0
16	37.699052011404525	40.0	37.0	41.0	32.0	41.0
17	37.17637092357226	39.0	36.0	40.0	32.0	41.0
18	36.40589216885663	37.0	36.0	39.0	31.0	41.0
19	35.62099651414561	35.0	34.0	39.0	31.0	41.0
20	35.294053060743956	35.0	34.0	39.0	30.0	41.0
21	35.23193984469776	35.0	33.0	39.0	30.0	41.0
22	35.18064506221815	35.0	33.0	39.0	30.0	41.0
23	34.793868786502934	35.0	33.0	39.0	28.0	41.0
24	34.64780329748517	35.0	33.0	39.0	27.0	41.0
25	34.60148903824202	35.0	33.0	39.0	27.0	41.0
26	34.579051090033325	35.0	33.0	39.0	27.0	41.0
27	34.50228295309709	35.0	33.0	39.0	25.0	41.0
28	34.37058317678554	35.0	33.0	39.0	25.0	41.0
29	34.33548661196452	35.0	33.0	39.0	25.0	41.0
30	34.1216773050916	35.0	33.0	39.0	24.0	41.0
31	33.94308485316926	35.0	33.0	39.0	24.0	41.0
32	33.69323201662563	35.0	33.0	39.0	22.0	41.0
33	33.36579972461239	35.0	33.0	39.0	21.0	41.0
34	33.144215068514185	35.0	33.0	39.0	21.0	40.0
35	33.00279482598881	35.0	33.0	39.0	20.0	40.0
36	32.7912607941196	35.0	33.0	39.0	20.0	40.0
37	32.6823982268723	35.0	33.0	39.0	18.0	40.0
38	32.4880324117915	35.0	33.0	39.0	17.0	40.0
39	32.293390185349175	35.0	33.0	38.0	15.0	40.0
40	31.918599413393665	35.0	31.0	38.0	15.0	40.0
41	31.646843535813186	35.0	31.0	38.0	13.0	40.0
42	31.486604286423596	35.0	31.0	38.0	12.0	40.0
43	31.3123013293339	35.0	31.0	38.0	10.0	40.0
44	31.105558427731225	35.0	30.0	38.0	10.0	40.0
45	30.959480141891166	35.0	29.0	38.0	10.0	40.0
46	30.694038216430098	35.0	28.0	38.0	8.0	40.0
47	30.262557521716207	35.0	26.0	38.0	8.0	40.0
48	30.06330076115499	35.0	25.0	38.0	8.0	40.0
49	29.960918504716908	35.0	26.0	38.0	7.0	40.0
50	29.708872292832243	35.0	24.0	38.0	7.0	40.0
51	28.942649761211296	34.0	23.0	36.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	11.0
14	25.0
15	59.0
16	153.0
17	416.0
18	781.0
19	1566.0
20	2413.0
21	3333.0
22	4494.0
23	5816.0
24	7344.0
25	8410.0
26	9271.0
27	10055.0
28	10973.0
29	11963.0
30	13555.0
31	16072.0
32	19364.0
33	24086.0
34	33392.0
35	71611.0
36	32436.0
37	29586.0
38	41997.0
39	31529.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.755995311244316	42.45678513111624	6.034725456974524	22.752494100664922
2	25.349481216824238	63.41081382671055	5.760873459902437	5.478831496562774
3	8.100388511524818	63.38854735592058	23.86095484769222	4.650109284862383
4	8.037684082288687	50.45710249230911	35.95804689779434	5.547166527607864
5	15.583970188522786	48.58748675528893	23.790060452188513	12.038482603999775
6	7.091742978383609	56.68966682193478	27.525964752432674	8.69262544724894
7	21.79580366603365	41.206279656635665	30.555996334990095	6.44192034234059
8	19.26024129688116	48.303397300382365	25.318768843320825	7.117592559415646
9	17.483018616817073	40.429768479891074	23.951812285973148	18.13540061731871
10	15.184197460086713	44.54957744892788	27.571009566904348	12.69521552408106
11	11.720609538239463	45.32890392657695	26.860785929637952	16.08970060554563
12	8.769406381007467	45.05454005661314	30.413951607536816	15.762101954842572
13	8.632992255363147	46.63545948270126	35.55494699556206	9.176601266373535
14	7.269874744703395	54.76860785929638	25.79916155220336	12.162355843796869
15	4.360645164592728	51.22030497386889	33.34954264157125	11.069507219967138
16	4.21578513623497	55.272034848306475	23.966912536278993	16.54526747917957
17	4.531866646874248	54.30075603626108	25.65327777806215	15.514099538802526
18	4.257758713356299	55.31247280675262	24.329830416510973	16.0999380633801
19	4.334539647114828	55.37927221912254	21.93119404589452	18.3549940878681
20	5.957944523215995	56.105875788924095	25.5793121452081	12.356867542651809
21	7.156750835632495	58.23936200162776	24.27429220775897	10.329594954980779
22	5.3334595953132915	51.457302122736884	24.270709097516903	18.938529184432923
23	7.015217981070941	54.35424675344619	24.585766862372736	14.044768403110139
24	12.88384068468118	49.33789241455562	20.869057795568207	16.909209105194996
25	10.458586923695108	58.162581067869226	17.841585577469402	13.537246430966263
26	10.823808231939845	50.378018130537825	20.376380137284308	18.42179350023802
27	14.610132012018775	50.10007115033195	18.201688156796905	17.088108680852372
28	10.557890264689473	47.19851966359714	23.878870398902542	18.364719672810846
29	17.690839010856823	45.57844196129217	19.771602315712965	16.95911671213804
30	12.431345048397583	50.712783001725015	20.491551537922103	16.3643204119553
31	11.808395739170049	48.17850031480183	22.8128951018883	17.200208844139823
32	11.01345713832341	50.75424470595462	24.57169035785034	13.660607797871632
33	7.783539191547955	53.37477797513321	21.654014875026235	17.187667958292597
34	11.932780851858867	54.76400100327087	20.94225561908467	12.360962525785597
35	8.441039920966825	50.77446368517769	18.35064316828845	22.433853225567027
36	10.817665757239164	53.68778824842215	20.43447771049493	15.060068283843755
37	7.3167111142960986	48.92865003762266	28.591172240109337	15.163466607971909
38	8.03384503560076	50.035319229528916	25.347689661703203	16.58314607316711
39	14.183741893213078	49.290288235625326	24.401492621352265	12.124477249809328
40	11.896437876546496	47.09844851326518	25.5759849714119	15.429128638776419
41	10.56352086649843	52.78279697585495	22.029985513997165	14.62369664364945
42	10.74344418793925	44.69955620620288	29.27529035989783	15.281709245960043
43	16.0879090504246	44.50222920644345	24.94023883989128	14.469622903240667
44	14.870931250351912	40.08527802376114	25.094568516745923	19.949222209141027
45	13.102666345892988	37.37695855365196	24.98835489171329	24.532020208741763
46	18.771658621731053	40.36757592354667	24.50924186506007	16.351523589662214
47	11.279119169127922	38.716273974846565	34.070771546009695	15.933835310015818
48	12.754848715966851	39.698046181172295	27.15536877882484	20.391736324036017
49	11.746970992163225	35.364018406949185	34.281919113845646	18.607091487041938
50	12.329994215836324	35.356340313573334	32.18835898669642	20.12530648389392
51	11.495385465881112	34.58929878532563	28.67742282236475	25.237892926428508
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	80330.0
1	52586.0
2	24842.0
3	26030.0
4	27218.0
5	17592.0
6	7966.0
7	7422.5
8	6879.0
9	6974.5
10	7070.0
11	7087.5
12	7105.0
13	6719.5
14	6334.0
15	5560.5
16	4787.0
17	4053.5
18	3320.0
19	2958.5
20	2597.0
21	2232.0
22	1867.0
23	1603.5
24	1340.0
25	1349.0
26	1380.0
27	1402.0
28	1463.5
29	1525.0
30	1699.5
31	1874.0
32	1970.5
33	2067.0
34	2336.5
35	2606.0
36	2819.5
37	3033.0
38	4259.5
39	5486.0
40	11002.5
41	16519.0
42	21791.5
43	27064.0
44	46278.0
45	65492.0
46	45872.5
47	26253.0
48	23683.5
49	21114.0
50	14476.0
51	7838.0
52	6620.5
53	5403.0
54	4549.0
55	3695.0
56	3520.5
57	3346.0
58	3086.5
59	2827.0
60	2831.0
61	2835.0
62	2549.0
63	2263.0
64	1947.0
65	1631.0
66	1332.0
67	1033.0
68	926.5
69	820.0
70	697.0
71	574.0
72	497.5
73	421.0
74	328.0
75	205.0
76	175.0
77	147.0
78	119.0
79	75.5
80	32.0
81	20.5
82	9.0
83	11.5
84	14.0
85	7.5
86	1.0
87	1.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	390722.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.30051033727305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.07912042509726	18.30406273514161
2	7.778912696682083	3.4694744601020675
3	2.971319706655343	1.9878583750083179
4	1.6285448681900083	1.4526952667113702
5	1.1534091561176594	1.286080640455362
6	0.788449841047594	1.0549700298421896
7	0.658763040409489	1.0283526394725662
8	0.4728403704681349	0.8435665255603729
9	0.3752883522890294	0.7532209601711702
>10	1.8397162957777193	6.8468117996939
>50	0.10902872620017674	1.747534052344122
>100	0.09869968898121263	4.523932617052533
>500	0.005738354010535618	0.8827248017772228
>1k	0.029839440854785213	16.110431457660436
>5k	0.004590683208428494	7.002677095223714
>10k+	0.005738354010535618	32.70560654378305
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76343	19.53895608642462	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18600	4.760417893028803	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	11952	3.0589524009397984	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10764	2.7548999032560233	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	10129	2.5923802601338033	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	9120	2.334140386259284	No Hit
AAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	7591	1.9428135605366474	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	5377	1.376170269398703	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	5273	1.3495528790290794	No Hit
GAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	4363	1.1166507132948746	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	4020	1.0288645123642897	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	3936	1.0073658509119015	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	3710	0.9495242141471429	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	3560	0.9111337472678784	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	3318	0.8491971273693317	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	3244	0.8302578303755611	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	3222	0.8246272285666023	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	3070	0.7857248887956143	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	2421	0.6196221354313297	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	2419	0.6191102625396062	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	2270	0.5809757321062035	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	2195	0.5617804986665711	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2140	0.5477039941441741	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT	2078	0.5318359345007448	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGT	1952	0.4995879423221625	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	1935	0.4952370227425126	No Hit
ACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	1911	0.4890945480418302	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	1863	0.47680959864046557	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA	1657	0.4240866907929423	No Hit
GAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	1572	0.4023320928946924	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	1513	0.38723184258884835	No Hit
AAAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	1243	0.3181290022061722	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	1229	0.3145458919641074	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1082	0.2769232344224282	No Hit
AAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	1024	0.2620789205624459	No Hit
GAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	985	0.25209739917383717	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC	900	0.23034280127558726	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG	530	0.1356463163067347	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTAACATCGT	523	0.13385476118570236	No Hit
GAAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	511	0.1307835238353612	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA	462	0.11824263798813478	No Hit
CGTTTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG	455	0.11645108286710244	No Hit
TGTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTT	454	0.11619514642124068	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTTAACA	439	0.11235609973331423	No Hit
AAAAAAAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGT	423	0.10826111659952602	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTAT	420	0.10749330726194072	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC	404	0.1033983241281525	No Hit
AGGGTAGGGTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	392	0.10032708677781134	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.559364458617636E-4	0.0	0.0	0.9886824903639928	0.0
2	2.559364458617636E-4	0.0	0.0	3.0233772349650136	0.0
3	2.559364458617636E-4	0.0	0.0	4.30433914650314	0.0
4	2.559364458617636E-4	0.0	0.0	6.239986486555659	0.0
5	2.559364458617636E-4	0.0	0.0	13.271840336607614	0.0
6	2.559364458617636E-4	0.0	0.0	17.525760003275987	0.0
7	2.559364458617636E-4	0.0	0.0	20.7927887347014	0.0
8	2.559364458617636E-4	0.0	0.0	23.217018749904025	0.0
9	2.559364458617636E-4	0.0	0.0	24.214403079427317	0.0
10	2.559364458617636E-4	0.0	0.0	28.13048663755816	0.0
11	2.559364458617636E-4	0.0	0.0	30.438009633447823	0.0
12	2.559364458617636E-4	0.0	0.0	34.14064219572995	0.0
13	2.559364458617636E-4	0.0	0.0	34.76563899652438	0.0
14	2.559364458617636E-4	0.0	0.0	35.12702125808119	0.0
15	5.118728917235272E-4	0.0	0.0	35.812163123653136	0.0
16	5.118728917235272E-4	0.0	0.0	36.24264822559262	0.0
17	5.118728917235272E-4	0.0	0.0	36.6088932796208	0.0
18	5.118728917235272E-4	0.0	0.0	36.9208798071263	0.0
19	5.118728917235272E-4	0.0	0.0	37.75728011220254	0.0
20	5.118728917235272E-4	0.0	0.0	38.051607024943564	0.0
21	5.118728917235272E-4	0.0	0.0	38.25046964337815	0.0
22	5.118728917235272E-4	0.0	0.0	38.507173898577506	0.0
23	5.118728917235272E-4	0.0	0.0	38.71678584773829	0.0
24	5.118728917235272E-4	0.0	0.0	38.89389386827463	0.0
25	5.118728917235272E-4	0.0	0.0	39.040289515307556	0.0
26	5.118728917235272E-4	0.0	0.0	39.168513674684306	0.0
27	5.118728917235272E-4	0.0	0.0	39.30390405454518	0.0
28	5.118728917235272E-4	0.0	0.0	39.43238415036778	0.0
29	5.118728917235272E-4	0.0	0.0	39.55395396215212	0.0
30	5.118728917235272E-4	0.0	0.0	39.681154375745415	0.0
31	5.118728917235272E-4	0.0	0.0	39.799908886625275	0.0
32	5.118728917235272E-4	0.0	0.0	39.92506180865168	0.0
33	5.118728917235272E-4	0.0	0.0	40.04279257374809	0.0
34	5.118728917235272E-4	0.0	0.0	40.16026740239864	0.0
35	5.118728917235272E-4	0.0	0.0	40.29463403647606	0.0
36	5.118728917235272E-4	0.0	0.0	40.40084766150869	0.0
37	5.118728917235272E-4	0.0	0.0	40.50552566786615	0.0
38	5.118728917235272E-4	0.0	0.0	40.62120894139567	0.0
39	5.118728917235272E-4	0.0	0.0	40.73689221492519	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATGA	60	0.0	45.000004	18
TAGGGTC	60	0.0	45.000004	5
CGAGATA	30	2.1618107E-6	45.000004	19
TGAGGGA	55	1.8189894E-12	45.000004	4
TCACGAA	20	7.027566E-4	45.0	6
GTTGATC	40	6.7957444E-9	45.0	16
TTGATCC	35	1.2091732E-7	45.0	17
ATCCTGC	35	1.2091732E-7	45.0	20
CGGGTTA	20	7.027566E-4	45.0	23
TCGTTGA	20	7.027566E-4	45.0	24
ACTGCTA	20	7.027566E-4	45.0	29
GGAGCAA	20	7.027566E-4	45.0	8
CATATGC	35	1.2091732E-7	45.0	33
TTAATCG	20	7.027566E-4	45.0	20
TGCCAGT	35	1.2091732E-7	45.0	24
TATGGGA	20	7.027566E-4	45.0	4
CGTTGAT	20	7.027566E-4	45.0	25
TCTTAGA	25	3.886063E-5	45.0	20
GTCTCAA	40	6.7957444E-9	45.0	42
ATTAATC	20	7.027566E-4	45.0	19
>>END_MODULE