SRR3552750_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	137912
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6610	4.792911421776205	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	2085	1.5118336330413598	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1721	1.2478972098149546	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	1586	1.1500087012007658	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1068	0.7744068681478044	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	905	0.6562155577469692	No Hit
AAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	701	0.5082951447299727	No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	616	0.4466616393062243	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	519	0.3763269331167701	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	454	0.32919542896919773	No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	420	0.3045420267996984	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	413	0.2994663263530367	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	408	0.2958408260339927	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	376	0.2726376239921109	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC	368	0.26683682348164045	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	360	0.26103602297117	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	349	0.25305992226927315	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	340	0.24653402169499392	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	336	0.24363362143975867	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	305	0.22115551946168568	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	287	0.20810371831312724	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	256	0.18562561633505426	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGT	221	0.16024711410174602	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	205	0.14864551308080515	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	176	0.1276176112303498	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	164	0.11891641046464412	No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	161	0.11674111027321771	No Hit
GCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	155	0.11239050989036486	No Hit
AAAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC	141	0.1022391089970416	No Hit
GACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	141	0.1022391089970416	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATGG	20	7.011686E-4	45.000004	2
AGCGTTG	20	7.011686E-4	45.000004	1
GAAATCT	30	2.1515825E-6	45.000004	9
GGAATGT	20	7.011686E-4	45.000004	26
GGGTGAT	20	7.011686E-4	45.000004	7
CGTATGG	20	7.011686E-4	45.000004	2
GATAAAG	30	2.1515825E-6	45.000004	1
TACCAGG	20	7.011686E-4	45.000004	2
CCGATTG	20	7.011686E-4	45.000004	11
TGGGTAT	20	7.011686E-4	45.000004	6
TAGGGTC	20	7.011686E-4	45.000004	5
TAAGGGA	30	2.1515825E-6	45.000004	4
CGACTGG	20	7.011686E-4	45.000004	2
TGAGCGG	30	2.1515825E-6	45.000004	2
AGGGATT	60	0.0	45.000004	6
TAATGGG	40	6.7393557E-9	45.000004	3
CCTAGGG	25	3.8729137E-5	45.0	3
AGGGTGC	25	3.8729137E-5	45.0	6
TACGGGA	25	3.8729137E-5	45.0	4
TGCGGGA	25	3.8729137E-5	45.0	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content