##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552750_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	137912
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.043984569870645	33.0	33.0	33.0	33.0	33.0
2	32.13428128081675	33.0	33.0	33.0	33.0	33.0
3	32.21364348280063	33.0	33.0	33.0	33.0	33.0
4	32.194877893149254	33.0	33.0	33.0	33.0	33.0
5	32.218385637217935	33.0	33.0	33.0	33.0	33.0
6	35.7684102906201	37.0	37.0	37.0	33.0	37.0
7	36.00524247346134	37.0	37.0	37.0	37.0	37.0
8	36.086308660595165	37.0	37.0	37.0	37.0	37.0
9	35.92869365972504	37.0	37.0	37.0	37.0	37.0
10	36.10234062300597	37.0	37.0	37.0	37.0	37.0
11	36.06983438714543	37.0	37.0	37.0	37.0	37.0
12	36.030874760716976	37.0	37.0	37.0	37.0	37.0
13	36.042505365740475	37.0	37.0	37.0	37.0	37.0
14	36.06422936365218	37.0	37.0	37.0	37.0	37.0
15	36.076048494692266	37.0	37.0	37.0	37.0	37.0
16	36.0889625268287	37.0	37.0	37.0	37.0	37.0
17	36.07004466616393	37.0	37.0	37.0	37.0	37.0
18	36.069870642148615	37.0	37.0	37.0	37.0	37.0
19	36.082349614246766	37.0	37.0	37.0	37.0	37.0
20	36.04356401183363	37.0	37.0	37.0	37.0	37.0
21	35.955761645107025	37.0	37.0	37.0	37.0	37.0
22	35.98823162596438	37.0	37.0	37.0	37.0	37.0
23	36.02768432043622	37.0	37.0	37.0	37.0	37.0
24	36.005401995475374	37.0	37.0	37.0	37.0	37.0
25	36.00767880967574	37.0	37.0	37.0	37.0	37.0
26	35.97119177446488	37.0	37.0	37.0	37.0	37.0
27	35.94402227507396	37.0	37.0	37.0	37.0	37.0
28	35.91972417193573	37.0	37.0	37.0	37.0	37.0
29	35.89805818202912	37.0	37.0	37.0	37.0	37.0
30	35.851804048958755	37.0	37.0	37.0	33.0	37.0
31	35.775189976216716	37.0	37.0	37.0	33.0	37.0
32	35.648326469052726	37.0	37.0	37.0	33.0	37.0
33	35.54692122512907	37.0	37.0	37.0	33.0	37.0
34	35.409115958002204	37.0	37.0	37.0	33.0	37.0
35	35.292867915772376	37.0	37.0	37.0	33.0	37.0
36	35.25633737455769	37.0	37.0	37.0	33.0	37.0
37	35.20308602587157	37.0	37.0	37.0	33.0	37.0
38	35.11521114913858	37.0	37.0	37.0	33.0	37.0
39	35.09007918092697	37.0	37.0	37.0	33.0	37.0
40	35.05110505249724	37.0	37.0	37.0	33.0	37.0
41	35.0084909217472	37.0	37.0	37.0	33.0	37.0
42	34.960699576541565	37.0	37.0	37.0	27.0	37.0
43	34.926532861534895	37.0	37.0	37.0	27.0	37.0
44	34.83306746330994	37.0	37.0	37.0	27.0	37.0
45	34.76374789720982	37.0	37.0	37.0	27.0	37.0
46	34.75073235106445	37.0	37.0	37.0	27.0	37.0
47	34.69921399153083	37.0	37.0	37.0	27.0	37.0
48	34.65705667382099	37.0	37.0	37.0	27.0	37.0
49	34.61037473171297	37.0	37.0	37.0	27.0	37.0
50	34.55979175126168	37.0	37.0	37.0	27.0	37.0
51	34.26173211903242	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	73.0
15	266.0
16	199.0
17	94.0
18	71.0
19	67.0
20	78.0
21	88.0
22	129.0
23	194.0
24	319.0
25	516.0
26	828.0
27	1386.0
28	2207.0
29	2624.0
30	2758.0
31	3075.0
32	3627.0
33	4862.0
34	7404.0
35	15018.0
36	92029.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.43265270607344	25.91072568014386	19.949678055571667	17.706943558211034
2	29.623238006844943	32.62297697082197	20.92493764139451	16.82884738093857
3	26.334184117408206	31.49762167179071	28.09690237252741	14.07129183827368
4	25.0246534021695	32.1473113289634	27.499419919948952	15.32861534891815
5	20.89665873890597	33.82446777655316	26.61552294216602	18.66335054237485
6	19.765502639364232	39.56000928128082	26.260949010963515	14.413539068391437
7	61.44787980741342	15.862288995881432	16.016010209408897	6.6738209872962475
8	58.95426068797494	16.029787110621267	15.95365160392134	9.062300597482452
9	54.61671210627067	16.28212193282673	19.360896803758916	9.740269157143686
10	29.68704681246012	29.28534137711004	25.464064040837638	15.563547769592203
11	21.742125413307036	30.794274609896167	26.90483786762573	20.558762109171067
12	18.8736295608794	27.502320320204188	34.58292244329718	19.041127675619236
13	19.028800974534484	29.488369394976505	34.46328093276872	17.019548697720285
14	15.11688613028598	32.942020998897846	30.768171007599047	21.172921863217123
15	13.45278148384477	32.40762225187076	34.91139277220257	19.228203492081906
16	13.924821625384304	32.71288937873427	30.641278496432506	22.721010499448923
17	14.3693079644991	32.92534369743024	29.695748013225824	23.00960032484483
18	14.610766285747435	31.995765415627357	32.9877023029178	20.40576599570741
19	14.950113115609954	33.42711294158594	30.00391554034457	21.61885840245954
20	16.126950519171647	35.0129067811358	30.272927664017637	18.587215035674923
21	17.18632171239631	35.479146122164856	30.115580950171122	17.218951215267708
22	13.61085329775509	34.50026103602297	26.945443471199027	24.943442195022914
23	14.314200359649632	33.47279424560589	28.323133592435756	23.88987180230872
24	18.058617089158304	31.599860780787747	27.76335634317536	22.57816578687859
25	14.701403793723532	35.42113811706015	25.361824931840594	24.515633157375717
26	13.576773594756075	35.49799872382388	29.08086315911596	21.844364522304076
27	18.120250594582053	31.747056093740937	30.246824061720517	19.885869249956496
28	15.836910493648123	31.158999941991993	28.357213295434768	24.646876268925112
29	16.21541272695632	31.646267184871512	26.83885376181913	25.299466326353038
30	18.14490399675155	31.30039445443471	28.195515981205403	22.359185567608332
31	19.65818782992053	32.876036893091246	25.920877081037187	21.54489819595104
32	19.12741458321248	32.45475375601833	24.50910725680144	23.90872440396775
33	17.84326237020709	28.33255989326527	28.130982075526422	25.693195661001216
34	16.922385289169906	28.37461569696618	30.01624224142932	24.686756772434595
35	18.553860432739718	28.779221532571498	27.176750391554034	25.490167643134754
36	19.886594350020303	28.945994547247523	31.062561633505425	20.104849469226753
37	19.41745460873601	28.03599396716747	32.746969081733276	19.799582342363244
38	19.211526190614308	28.822002436336213	26.56694123789083	25.399530135158656
39	18.993271071407854	25.18997621671791	30.77397180810952	25.042780903764722
40	20.737136724868034	25.471315041475723	31.869598004524626	21.92195022913162
41	19.206450490167644	27.248535297871108	29.800162422414296	23.74485178954696
42	20.289749985498	26.424096525320493	32.75204478217994	20.534108707001568
43	21.402053483380705	24.559139161204246	32.48158245837926	21.55722489703579
44	18.227565404025757	25.35747433145774	29.751580718139103	26.663379546377403
45	19.60235512500725	23.501943268171008	28.500783108068912	28.39491849875283
46	21.687742908521376	25.053657404721854	30.933493822147458	22.325105864609316
47	16.308950635187657	25.712048262660247	35.4494170195487	22.5295840826034
48	17.390799930390394	24.250246534021695	32.45185335576309	25.907100179824816
49	20.115725970183885	21.581153199141482	35.22898660015082	23.074134230523814
50	18.9968965717269	20.898834039097395	32.62225187075816	27.48201751841754
51	17.202273913800102	20.578339810893905	29.84439352630663	32.37499274899936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7170.0
1	4419.0
2	1668.0
3	2301.0
4	2934.0
5	2026.0
6	1118.0
7	1051.5
8	985.0
9	956.5
10	928.0
11	953.0
12	978.0
13	957.5
14	937.0
15	923.0
16	909.0
17	811.0
18	713.0
19	686.5
20	660.0
21	634.5
22	609.0
23	664.0
24	719.0
25	977.0
26	1173.0
27	1111.0
28	1248.0
29	1385.0
30	1486.0
31	1587.0
32	1842.0
33	2097.0
34	2145.5
35	2194.0
36	2379.0
37	2564.0
38	2785.5
39	3007.0
40	3550.0
41	4093.0
42	5281.0
43	6469.0
44	7453.0
45	8437.0
46	11116.0
47	13795.0
48	16307.0
49	18819.0
50	18375.5
51	17932.0
52	14233.0
53	10534.0
54	8504.0
55	6474.0
56	5352.0
57	4230.0
58	3657.0
59	3084.0
60	2803.5
61	2523.0
62	2220.5
63	1918.0
64	1672.0
65	1426.0
66	1184.5
67	943.0
68	773.0
69	603.0
70	517.5
71	432.0
72	362.5
73	293.0
74	225.0
75	137.5
76	118.0
77	99.0
78	80.0
79	56.0
80	32.0
81	17.5
82	3.0
83	5.5
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	137912.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.904025755554265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.62092076588863	41.06459191368409
2	11.614424144405916	12.288995881431637
3	4.605200038376667	7.30900864319276
4	2.323158947931087	4.916178432623703
5	1.259577034305999	3.3318347932014616
6	0.8100217924644673	2.5712048262660248
7	0.4824495278299365	1.7866465572248973
8	0.2713778594043393	1.148558501073148
9	0.18777154918381053	0.8940483786762573
>10	0.7346390537410397	6.913829108416962
>50	0.041117857485505956	1.4668774290852138
>100	0.03700607173695536	4.843668426242822
>500	0.0054823809980674605	1.9874992748999363
>1k	0.0054823809980674605	4.684146412204885
>5k	0.0013705952495168651	4.792911421776205
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6610	4.792911421776205	No Hit
CCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	2085	1.5118336330413598	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1721	1.2478972098149546	No Hit
CTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGCT	1586	1.1500087012007658	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	1068	0.7744068681478044	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCC	905	0.6562155577469692	No Hit
AAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	701	0.5082951447299727	No Hit
ACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	616	0.4466616393062243	No Hit
GAATCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	519	0.3763269331167701	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	454	0.32919542896919773	No Hit
CGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	420	0.3045420267996984	No Hit
TCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTGC	413	0.2994663263530367	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	408	0.2958408260339927	No Hit
AAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	376	0.2726376239921109	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC	368	0.26683682348164045	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	360	0.26103602297117	No Hit
CGTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTC	349	0.25305992226927315	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCG	340	0.24653402169499392	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	336	0.24363362143975867	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	305	0.22115551946168568	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGC	287	0.20810371831312724	No Hit
CGTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	256	0.18562561633505426	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGT	221	0.16024711410174602	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	205	0.14864551308080515	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTT	176	0.1276176112303498	No Hit
AAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCT	164	0.11891641046464412	No Hit
ACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	161	0.11674111027321771	No Hit
GCCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCTG	155	0.11239050989036486	No Hit
AAAAAAACTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTC	141	0.1022391089970416	No Hit
GACCTGTCTCTTATACACATCTGACGCATGCCTGTTCGTATGCCGTCTTCT	141	0.1022391089970416	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1681362027959858	0.0
2	0.0	0.0	0.0	4.24981147398341	0.0
3	0.0	0.0	0.0	5.2286965601252975	0.0
4	0.0	0.0	0.0	6.384506061836533	0.0
5	0.0	0.0	0.0	9.975201577817739	0.0
6	0.0	0.0	0.0	11.94674865131388	0.0
7	0.0	0.0	0.0	14.025610534253728	0.0
8	0.0	0.0	0.0	16.99271999535936	0.0
9	0.0	0.0	0.0	18.260920006960962	0.0
10	0.0	0.0	0.0	21.222228667556124	0.0
11	0.0	0.0	0.0	25.332095829224432	0.0
12	0.0	0.0	0.0	28.368089796391903	0.0
13	0.0	0.0	0.0	29.472417193572714	0.0
14	0.0	0.0	0.0	29.95388363594176	0.0
15	0.0	0.0	0.0	30.72393990370671	0.0
16	0.0	0.0	0.0	32.25607633853472	0.0
17	0.0	0.0	0.0	33.90495388363594	0.0
18	0.0	0.0	0.0	35.70827774232844	0.0
19	0.0	0.0	0.0	36.85031034282731	0.0
20	0.0	0.0	0.0	37.846597830500606	0.0
21	0.0	0.0	0.0	38.97920413016996	0.0
22	0.0	0.0	0.0	40.1843204362202	0.0
23	0.0	0.0	0.0	41.292998433783865	0.0
24	0.0	0.0	0.0	42.25375601833053	0.0
25	7.251000638088056E-4	0.0	0.0	43.012935785138346	0.0
26	7.251000638088056E-4	0.0	0.0	43.71773304716051	0.0
27	7.251000638088056E-4	0.0	0.0	44.41745460873601	0.0
28	7.251000638088056E-4	0.0	0.0	45.13022797146006	0.0
29	7.251000638088056E-4	0.0	0.0	45.84010093392888	0.0
30	7.251000638088056E-4	0.0	0.0	46.52314519403678	0.0
31	7.251000638088056E-4	0.0	0.0	47.18226115203898	0.0
32	7.251000638088056E-4	0.0	0.0	47.892134114507805	0.0
33	7.251000638088056E-4	0.0	0.0	48.51572016938338	0.0
34	7.251000638088056E-4	0.0	0.0	49.18426242821509	0.0
35	7.251000638088056E-4	0.0	0.0	49.862956087940134	0.0
36	7.251000638088056E-4	0.0	0.0	50.433609838157665	0.0
37	7.251000638088056E-4	0.0	0.0	50.97525958582284	0.0
38	7.251000638088056E-4	0.0	0.0	51.50893323278613	0.0
39	7.251000638088056E-4	0.0	0.0	52.0832124833227	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATGG	20	7.011686E-4	45.000004	2
AGCGTTG	20	7.011686E-4	45.000004	1
GAAATCT	30	2.1515825E-6	45.000004	9
GGAATGT	20	7.011686E-4	45.000004	26
GGGTGAT	20	7.011686E-4	45.000004	7
CGTATGG	20	7.011686E-4	45.000004	2
GATAAAG	30	2.1515825E-6	45.000004	1
TACCAGG	20	7.011686E-4	45.000004	2
CCGATTG	20	7.011686E-4	45.000004	11
TGGGTAT	20	7.011686E-4	45.000004	6
TAGGGTC	20	7.011686E-4	45.000004	5
TAAGGGA	30	2.1515825E-6	45.000004	4
CGACTGG	20	7.011686E-4	45.000004	2
TGAGCGG	30	2.1515825E-6	45.000004	2
AGGGATT	60	0.0	45.000004	6
TAATGGG	40	6.7393557E-9	45.000004	3
CCTAGGG	25	3.8729137E-5	45.0	3
AGGGTGC	25	3.8729137E-5	45.0	6
TACGGGA	25	3.8729137E-5	45.0	4
TGCGGGA	25	3.8729137E-5	45.0	4
>>END_MODULE