FastQCFastQC Report
Sat 18 Jun 2016
SRR3552749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289247
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC84280.6537149204147847Illumina PCR Primer Index 7 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC65340.5068074620301618Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT64980.5040151344156706TruSeq Adapter, Index 23 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC38820.3011059944293064Illumina PCR Primer Index 7 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC25570.1983328252848368TruSeq Adapter, Index 23 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC16010.12418101418890251No Hit
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG15790.12247459175782453Illumina PCR Primer Index 7 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA14250.11052963474027862No Hit
CGCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG13620.10564306141491893TruSeq Adapter, Index 23 (95% over 24bp)
GAGGATGGGGGGTAGGGGAGAATTACAGGAAATTTCAGGTATGCAATCAAG13540.10502254416725422No Hit
ACCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG13360.1036263803600086TruSeq Adapter, Index 23 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTGCG650.045.0000041
ACGTTAG700.045.01
GACGTAA207.0336065E-445.012
TAGAACG207.0336065E-445.012
TACGCAT502.1827873E-1145.016
TACCTCG207.0336065E-445.010
TCGTAAG351.2120836E-745.01
TATCGCG253.8910704E-545.01
TCGAACA207.0336065E-445.013
TATCCGT207.0336065E-445.028
CATGCGG1500.043.52
TTACGAG1450.041.896551
CGAATAT700.041.78571314
TCGATAG556.184564E-1140.9090921
TATGGGA4950.040.4545444
AGTAACG950.040.2631571
AACACGT950.040.26315741
CCCTCGT900.040.014
CGATAAC451.9286745E-840.010
TCAACGG451.9286745E-840.02
Produced by FastQC (version 0.11.5)