FastQCFastQC Report
Sat 18 Jun 2016
SRR3552746_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552746_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693872
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC50080.7217469504461919No Hit
GCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC42340.6101989992390527No Hit
CTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGCT40330.5812311204371988No Hit
TCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC17800.256531464016418No Hit
ACTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTGC13120.18908386561210136No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCC12330.1776984804113727No Hit
GCCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG8930.12869808840823668No Hit
CGCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG7650.11025088200705606No Hit
ACCTGTCTCTTATACACATCTGACGCGAACGGAATCGTATGCCGTCTTCTG7230.10419789240666867No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTTG207.031351E-445.0000041
ACGCATT406.8084773E-945.00000417
CCGATCA207.031351E-445.00000411
CCGTGTT207.031351E-445.00000421
TTCACCG351.2109922E-745.00000423
GTTAGCG406.8084773E-945.0000041
CACGACG207.031351E-445.0000041
CGACAAT351.2109922E-745.00000420
TCGATAG351.2109922E-745.0000041
TCATCGA351.2109922E-745.00000416
TTAGTCG207.031351E-445.0000041
CATCGAC351.2109922E-745.00000417
ACGTAGC207.031351E-445.00000444
CACCGCG207.031351E-445.00000427
CGCCGCT207.031351E-445.00000413
TGTACGA207.031351E-445.00000419
CGATCAT207.031351E-445.00000410
ATCGACA351.2109922E-745.00000418
CTGTGCG207.031351E-445.0000041
GGCTACG406.8084773E-945.0000041