##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552743_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2235865
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.769464614366253	31.0	31.0	34.0	30.0	34.0
2	31.934524669423244	33.0	31.0	34.0	30.0	34.0
3	31.977498641465385	33.0	31.0	34.0	30.0	34.0
4	35.59659863184942	37.0	35.0	37.0	33.0	37.0
5	35.466002196018096	37.0	35.0	37.0	33.0	37.0
6	35.445906617796695	37.0	35.0	37.0	33.0	37.0
7	35.81456438559573	37.0	35.0	37.0	35.0	37.0
8	35.79452918669061	37.0	35.0	37.0	35.0	37.0
9	37.38673980763597	39.0	37.0	39.0	35.0	39.0
10	36.8606999975401	39.0	37.0	39.0	32.0	39.0
11	36.66021651575565	39.0	35.0	39.0	32.0	39.0
12	36.504359610262696	39.0	35.0	39.0	32.0	39.0
13	36.446757295274985	39.0	35.0	39.0	32.0	39.0
14	37.512320734928096	39.0	36.0	41.0	32.0	41.0
15	37.58151766765882	39.0	36.0	41.0	32.0	41.0
16	37.62486822773289	39.0	36.0	41.0	33.0	41.0
17	37.60562377424397	39.0	36.0	41.0	33.0	41.0
18	37.567372359243514	39.0	36.0	41.0	32.0	41.0
19	37.548507624565886	39.0	36.0	41.0	32.0	41.0
20	37.48767702880093	39.0	36.0	41.0	32.0	41.0
21	37.418726085877275	39.0	36.0	41.0	32.0	41.0
22	37.357315848676016	39.0	35.0	41.0	32.0	41.0
23	37.316524029849745	39.0	35.0	41.0	32.0	41.0
24	37.28701151455924	39.0	35.0	41.0	32.0	41.0
25	37.238766651832734	39.0	35.0	41.0	32.0	41.0
26	37.15498878510107	39.0	35.0	41.0	32.0	41.0
27	37.06198003904529	39.0	35.0	41.0	31.0	41.0
28	36.96538923414428	39.0	35.0	41.0	31.0	41.0
29	36.94611034208237	39.0	35.0	41.0	31.0	41.0
30	36.84724838038075	39.0	35.0	40.0	31.0	41.0
31	36.736508241776676	39.0	35.0	40.0	31.0	41.0
32	36.62531727094436	39.0	35.0	40.0	30.0	41.0
33	36.51045702669884	39.0	35.0	40.0	30.0	41.0
34	36.37136007764333	38.0	35.0	40.0	30.0	41.0
35	36.34446578840851	38.0	35.0	40.0	30.0	41.0
36	36.28069762709287	38.0	35.0	40.0	30.0	41.0
37	36.20827599161846	38.0	35.0	40.0	30.0	41.0
38	36.12590473932907	38.0	35.0	40.0	30.0	41.0
39	36.05966773485877	38.0	35.0	40.0	30.0	41.0
40	35.94251128757774	38.0	35.0	40.0	29.0	41.0
41	35.86112175824569	38.0	35.0	40.0	29.0	41.0
42	35.765597654599006	38.0	34.0	40.0	29.0	41.0
43	35.69377936503322	38.0	34.0	40.0	29.0	41.0
44	35.55196758301597	38.0	34.0	40.0	28.0	41.0
45	35.466148448139755	38.0	34.0	40.0	28.0	41.0
46	35.334512593559985	38.0	34.0	40.0	27.0	41.0
47	35.21912593112733	37.0	34.0	40.0	27.0	41.0
48	35.14361824170958	37.0	34.0	40.0	27.0	41.0
49	35.08793062192932	37.0	34.0	40.0	27.0	41.0
50	34.97893969448066	37.0	34.0	40.0	27.0	41.0
51	33.43926220948045	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	12.0
11	11.0
12	12.0
13	34.0
14	39.0
15	105.0
16	205.0
17	386.0
18	837.0
19	1556.0
20	2490.0
21	3734.0
22	5545.0
23	7944.0
24	11263.0
25	14950.0
26	20017.0
27	25006.0
28	31902.0
29	39161.0
30	50011.0
31	63785.0
32	81439.0
33	109051.0
34	167670.0
35	190526.0
36	207638.0
37	287910.0
38	432809.0
39	479725.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.180066775051266	20.385980370013396	26.329630814024995	10.10432204091034
2	31.42774720298408	24.020681033962248	25.970440970273252	18.581130792780424
3	33.333363150279645	24.255847289527765	25.804107135269795	16.60668242492279
4	29.56788535980482	26.157303772812757	25.223660641407243	19.05115022597518
5	25.6416644117601	31.12795271628654	24.07743759126781	19.15294528068555
6	27.114069946083507	36.421250835806276	21.99122040015833	14.47345881795189
7	82.08639609278735	6.578930302142572	7.014466436927096	4.320207168142979
8	83.29067273739693	5.4855726978149395	6.587562308099996	4.636192256688127
9	77.3011787384301	7.57456286493147	8.865964626665743	6.258293769972695
10	43.28776558513148	26.387952761011956	15.034941733959787	15.289339919896774
11	31.778215589939464	26.31165119539865	21.655243049110748	20.25489016555114
12	27.507430010309207	23.70223604734633	28.197677408967	20.59265653337746
13	24.45545683661581	25.410970698141433	29.840263164368153	20.293309300874608
14	21.82618360231946	26.98454513130265	28.46477761403305	22.724493652344844
15	21.835799567505195	25.447958620041906	31.692476960818293	21.023764851634603
16	23.910835403747544	25.991238290326113	28.771728167845556	21.326198138080787
17	23.775541009855246	24.923731978451293	26.97510806779479	24.325618943898668
18	25.076916540131002	24.336666122507395	27.880753086613012	22.70566425074859
19	25.461644598399275	26.170050517361286	25.826156767067783	22.542148117171653
20	26.47069478702873	26.32842322769935	26.554510223112754	20.646371762159163
21	25.741894076789073	26.126130155443196	27.01008334581918	21.12189242194855
22	23.923716324554476	25.048918427543704	25.921153558018933	25.106211689882883
23	23.88516301297261	26.51188689835925	26.254089580542654	23.34886050812549
24	23.74776652436529	24.704666873894443	27.737452842635847	23.810113759104418
25	22.9645797040519	26.265852365862873	25.992266974973894	24.777300955111333
26	22.972004123683675	26.61636547823773	25.993385110460604	24.41824528761799
27	23.562737464023993	26.76941586365903	26.417695164958527	23.25015150735845
28	21.297081890006776	27.367528898211653	27.125251300950637	24.21013791083093
29	23.040747093406804	25.97433208176701	25.488435124660924	25.496485700165263
30	24.193589505627575	26.8399478501609	24.984603274347958	23.981859369863564
31	24.94184577333605	28.023561350976024	23.17961952085658	23.85497335483135
32	24.44646702730263	27.633958221985676	23.40812168892129	24.5114530617904
33	23.544623669139238	29.10354605488256	23.04360952025279	24.30822075572541
34	22.267444590795957	27.543747050917656	25.663132613104995	24.525675745181395
35	22.852676704541643	27.053019748508966	24.57402392362687	25.520279623322516
36	23.18995109275381	31.02150621795144	23.324932408709827	22.46361028058492
37	24.015805963240176	28.971471891192003	24.227580824423654	22.785141321144163
38	23.48227643440011	29.054616445983996	24.007576486057967	23.45553063355793
39	23.665024498348515	27.389623255429107	24.576796899633923	24.368555346588458
40	23.82196599526358	26.598206957933506	26.653934830591293	22.925892216211626
41	21.715354012876446	27.889742895926183	24.64580822187386	25.749094869323507
42	23.341972793527336	26.920051076429036	25.1890431667386	24.548932963305027
43	23.802286810697424	24.96581859817118	26.37900767711825	24.852886914013144
44	22.73540665469516	25.60923848264542	25.24065630080528	26.414698561854138
45	23.60527133793856	24.839424562753116	24.866483441531578	26.688820657776745
46	23.70254912528261	26.646555136378986	24.952669324847427	24.698226413490975
47	21.745633121856642	27.196588345002944	27.20267100205066	23.855107531089757
48	22.079508378189203	26.323413980718875	26.82259438740711	24.774483253684814
49	22.935508181397356	25.950359256931883	27.38617939813003	23.727953163540732
50	21.426561979368163	25.48982161266445	27.215059943243443	25.86855646472394
51	21.75511491078397	24.97096202141006	24.975658190454254	28.29826487735172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	800.0
1	1117.5
2	1435.0
3	2376.5
4	3318.0
5	2584.5
6	1851.0
7	1892.0
8	1933.0
9	2210.0
10	2487.0
11	2543.5
12	2600.0
13	2886.5
14	3173.0
15	3553.0
16	3933.0
17	4021.5
18	4110.0
19	4357.5
20	4605.0
21	5287.5
22	5970.0
23	6631.0
24	7292.0
25	10202.5
26	16036.5
27	18960.0
28	23560.5
29	28161.0
30	31709.0
31	35257.0
32	38268.0
33	41279.0
34	45827.0
35	50375.0
36	52436.0
37	54497.0
38	61494.0
39	68491.0
40	73673.5
41	78856.0
42	92732.5
43	106609.0
44	116888.5
45	127168.0
46	150037.5
47	172907.0
48	200741.0
49	228575.0
50	224222.0
51	219869.0
52	200257.5
53	180646.0
54	163132.0
55	145618.0
56	136602.5
57	127587.0
58	117011.5
59	106436.0
60	102918.0
61	99400.0
62	92287.0
63	85174.0
64	74269.5
65	63365.0
66	53859.5
67	44354.0
68	38283.0
69	32212.0
70	26918.5
71	21625.0
72	18908.0
73	16191.0
74	13417.5
75	9041.0
76	7438.0
77	5600.5
78	3763.0
79	2885.0
80	2007.0
81	1534.0
82	1061.0
83	725.0
84	389.0
85	273.5
86	158.0
87	112.5
88	67.0
89	53.0
90	39.0
91	37.0
92	35.0
93	30.0
94	25.0
95	15.5
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2235865.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.880244126774077
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.64399123177313	14.58186797733787
2	12.849741690274103	5.623109702983678
3	5.712470719099528	3.74970761702839
4	2.89943874789652	2.5376171053841605
5	1.8307291767583995	2.0028400658740955
6	1.1637195084633427	1.5277480164160482
7	0.8276076346673273	1.2675779961392222
8	0.5994585618452395	1.049303974164699
9	0.471306622660748	0.9281073566144302
>10	4.806810841539898	26.55177284324158
>50	1.9508409645003937	29.241562510261897
>100	0.23097285357502184	7.460109390772265
>500	0.008197744092969432	1.1188119047431582
>1k	0.004098872046484716	1.5675474049742117
>5k	6.148308069727075E-4	0.7923161340644034
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	7445	0.33298074794318977	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	5144	0.23006755774610724	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	5080	0.2272051309001214	Illumina Single End Adapter 1 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	3787	0.16937516352731494	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	3502	0.1566284189787845	Illumina Single End Adapter 1 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2794	0.1249628219950668	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2339	0.10461275613688661	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.341762584055835E-4	0.0	0.0	0.2454978274627493	0.0
2	1.341762584055835E-4	0.0	0.0	1.118895818844161	0.0
3	1.341762584055835E-4	0.0	0.0	1.5313536371829248	0.0
4	1.341762584055835E-4	0.0	0.0	1.9772660692841473	0.0
5	1.341762584055835E-4	0.0	0.0	3.2032792677554323	0.0
6	1.341762584055835E-4	0.0	0.0	3.878543650891266	0.0
7	1.341762584055835E-4	0.0	0.0	4.63458214158726	0.0
8	1.341762584055835E-4	0.0	0.0	5.843376053563162	0.0
9	1.341762584055835E-4	0.0	0.0	6.2391065650206965	0.0
10	1.341762584055835E-4	0.0	0.0	7.315021255755602	0.0
11	1.341762584055835E-4	0.0	0.0	8.976570589011411	0.0
12	1.341762584055835E-4	0.0	0.0	10.156203527493833	0.0
13	1.341762584055835E-4	0.0	0.0	10.63664398342476	0.0
14	1.341762584055835E-4	0.0	0.0	10.812996312389165	0.0
15	1.341762584055835E-4	0.0	0.0	11.190568303542477	0.0
16	1.7890167787411136E-4	0.0	0.0	12.009043479816537	0.0
17	1.7890167787411136E-4	0.0	0.0	12.990006105019757	0.0
18	1.7890167787411136E-4	0.0	0.0	14.067978165050215	0.0
19	1.7890167787411136E-4	0.0	0.0	14.72195324851903	0.0
20	1.7890167787411136E-4	0.0	0.0	15.354594306901356	0.0
21	3.578033557482227E-4	0.0	0.0	16.121679976206078	0.0
22	4.025287752167506E-4	0.0	0.0	16.91940255784674	0.0
23	4.472541946852784E-4	0.0	0.0	17.740427083030504	0.0
24	8.050575504335012E-4	0.0	0.0	18.41139782589736	0.0
25	9.839592283076126E-4	0.0	0.0	19.016488025887075	0.0
26	9.839592283076126E-4	0.0	0.0	19.55829175732882	0.0
27	9.839592283076126E-4	0.0	0.0	20.098217021152887	0.0
28	9.839592283076126E-4	0.0	0.0	20.63313303799648	0.0
29	0.0010286846477761404	0.0	0.0	21.21304282682541	0.0
30	0.0010286846477761404	0.0	0.0	21.87878069561445	0.0
31	0.001073410067244668	0.0	0.0	22.47658065223079	0.0
32	0.001073410067244668	0.0	0.0	23.067492894248982	0.0
33	0.0011628609061817238	0.0	0.0	23.678352673350137	0.0
34	0.002146820134489336	0.0	0.0	24.246857480214594	0.0
35	0.002146820134489336	0.0	0.0	24.853826147821984	0.0
36	0.002191545553957864	0.0	0.0	25.434764621298694	0.0
37	0.002191545553957864	0.0	0.0	26.023082788987708	0.0
38	0.002459898070769031	0.0	0.0	26.615649871526234	0.0
39	0.002459898070769031	0.0	0.0	27.2492301637174	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGA	20	7.03472E-4	45.0	41
TACGCAC	20	7.03472E-4	45.0	1
CAATCGA	35	1.2126111E-7	45.0	31
CGAATAT	220	0.0	40.909092	14
CGTTAGG	275	0.0	40.090908	2
GTCGACG	45	1.9297659E-8	40.0	1
TACGAAT	225	0.0	40.0	12
AAGCACG	80	0.0	39.375	1
GCGTTAG	155	0.0	39.19355	1
TACGGGA	670	0.0	38.955223	4
GCGAGAC	875	0.0	38.057144	21
AACACGT	770	0.0	37.987015	41
CACGACC	880	0.0	37.840908	27
CGTATGG	250	0.0	37.8	2
TACGGGT	150	0.0	37.5	4
CGTATAG	30	1.1402673E-4	37.499996	2
TGTTACG	80	0.0	36.5625	1
CACAACG	770	0.0	36.23377	12
GCGTAAG	230	0.0	36.195652	1
CAATTCG	25	0.002107534	36.0	31
>>END_MODULE