FastQCFastQC Report
Sat 18 Jun 2016
SRR3552742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898896
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC70950.37363815606541906Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC68480.3606305979895687Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT56400.2970146864283247Illumina Single End Adapter 1 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA27110.14276716576368587No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC25870.1362370556365383Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG21310.11222310226573756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG302.1662527E-645.00000413
GTCGAAC207.034451E-445.010
CGTAATA207.034451E-445.034
TACGGGT1300.041.5384644
ACGTAAG800.039.3751
GATCGAC356.2504405E-638.5714310
ATTCGAC700.038.5714342
TCTAGCG700.038.571431
GCGTACG1350.038.3333321
CGTTAGG3050.036.8852422
CGTAAGG3850.036.8181842
CGTATGG2400.036.5625042
AACACGT6400.036.21093841
CGCATTA250.002107453536.00000417
TCGCTAA250.002107453536.00000426
CGAATAT2000.036.00000414
TTACGCG250.002107453536.0000041
CGGTCAT250.002107453536.00000434
GACCGAT7900.035.8860749
AGGGTAC3950.035.8860746
Produced by FastQC (version 0.11.5)