##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552742_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1898896
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.077113227896632	33.0	33.0	33.0	27.0	33.0
2	31.31540589900658	33.0	33.0	33.0	27.0	33.0
3	31.44596281207607	33.0	33.0	33.0	27.0	33.0
4	31.394983716854426	33.0	33.0	33.0	27.0	33.0
5	31.524775975092897	33.0	33.0	33.0	27.0	33.0
6	34.80957935558345	37.0	37.0	37.0	27.0	37.0
7	35.109338268130536	37.0	37.0	37.0	33.0	37.0
8	35.20564528020492	37.0	37.0	37.0	33.0	37.0
9	35.086958422156876	37.0	37.0	37.0	33.0	37.0
10	35.150372110952894	37.0	37.0	37.0	33.0	37.0
11	35.12979278486026	37.0	37.0	37.0	33.0	37.0
12	35.14848469847743	37.0	37.0	37.0	33.0	37.0
13	35.20720302744331	37.0	37.0	37.0	33.0	37.0
14	35.28857715219791	37.0	37.0	37.0	33.0	37.0
15	35.31502409821286	37.0	37.0	37.0	33.0	37.0
16	35.334998862496946	37.0	37.0	37.0	33.0	37.0
17	35.34679571709035	37.0	37.0	37.0	33.0	37.0
18	35.331032347216485	37.0	37.0	37.0	33.0	37.0
19	35.32408989223212	37.0	37.0	37.0	33.0	37.0
20	35.30543958173591	37.0	37.0	37.0	33.0	37.0
21	35.2435799538258	37.0	37.0	37.0	33.0	37.0
22	35.2721449726578	37.0	37.0	37.0	33.0	37.0
23	35.28473070668431	37.0	37.0	37.0	33.0	37.0
24	35.28108911704485	37.0	37.0	37.0	33.0	37.0
25	35.2807657712692	37.0	37.0	37.0	33.0	37.0
26	35.2276791356662	37.0	37.0	37.0	33.0	37.0
27	35.21758063632763	37.0	37.0	37.0	33.0	37.0
28	35.18782966523706	37.0	37.0	37.0	33.0	37.0
29	35.18531293972919	37.0	37.0	37.0	33.0	37.0
30	35.17096670907728	37.0	37.0	37.0	33.0	37.0
31	35.1566394368096	37.0	37.0	37.0	33.0	37.0
32	35.145031112815026	37.0	37.0	37.0	33.0	37.0
33	35.11666147066506	37.0	37.0	37.0	33.0	37.0
34	35.08618586799909	37.0	37.0	37.0	33.0	37.0
35	35.0827249096317	37.0	37.0	37.0	33.0	37.0
36	35.063586420741316	37.0	37.0	37.0	33.0	37.0
37	35.0617111205669	37.0	37.0	37.0	33.0	37.0
38	35.03472596708824	37.0	37.0	37.0	33.0	37.0
39	35.020270725726945	37.0	37.0	37.0	27.0	37.0
40	35.00213387147058	37.0	37.0	37.0	27.0	37.0
41	34.99205117078555	37.0	37.0	37.0	27.0	37.0
42	34.98496758116295	37.0	37.0	37.0	27.0	37.0
43	34.96671065713973	37.0	37.0	37.0	27.0	37.0
44	34.940738197352566	37.0	37.0	37.0	27.0	37.0
45	34.724739532865414	37.0	37.0	37.0	27.0	37.0
46	34.85758356434476	37.0	37.0	37.0	27.0	37.0
47	34.83493303477389	37.0	37.0	37.0	27.0	37.0
48	34.85467766533817	37.0	37.0	37.0	27.0	37.0
49	34.84697424187528	37.0	37.0	37.0	27.0	37.0
50	34.84049363419587	37.0	37.0	37.0	27.0	37.0
51	34.41659838137528	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1315.0
15	5629.0
16	6260.0
17	5859.0
18	5637.0
19	5731.0
20	5884.0
21	6622.0
22	7369.0
23	8702.0
24	10606.0
25	13150.0
26	16793.0
27	21023.0
28	25893.0
29	30698.0
30	36522.0
31	44782.0
32	56236.0
33	75539.0
34	112556.0
35	230152.0
36	1165937.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.25845543937109	18.306163160067747	22.6929752866929	13.742406113868268
2	29.942082136146475	23.587969009361228	25.474854863036207	20.995093991456088
3	33.168061863314264	24.415871116690962	24.709620748055713	17.706446271939065
4	29.941502852183582	26.043395741525604	24.384221147445672	19.63088025884514
5	25.75091000244353	30.868146544097204	23.75690927781195	19.624034175647324
6	26.83201186373556	36.33221619298793	21.671223700508087	15.164548242768431
7	79.80563443179616	7.630907643177931	7.601943445033324	4.961514479992585
8	81.10897068612499	6.400350519459728	7.098282370387847	5.392396424027435
9	75.56064155172268	8.233836924191742	9.515423698822895	6.690097825262679
10	42.66015621708614	26.44399693295473	15.389573731262796	15.506273118696337
11	31.579033290922727	26.40644879972363	21.676542580531002	20.33797532882264
12	27.481125875245404	23.86660459551234	27.994213479832492	20.658056049409762
13	24.58065107304455	25.322608505152466	29.53084318467151	20.56589723713147
14	21.91710341166657	27.000004212974275	28.11870686967585	22.9641855056833
15	21.72904677244041	25.338038523436772	31.586879955510994	21.346034748611824
16	23.93074712885803	25.59581988692377	28.725111854467016	21.748321129751183
17	23.757488561774842	24.67654889999242	27.09653398606348	24.469428552169262
18	24.92832677513671	24.15234957575349	28.226453686773787	22.692869962336008
19	25.34604317456038	25.756650179894002	26.185478298969507	22.711828346576116
20	26.408397300326087	26.049451892046747	26.667916515701755	20.874234291925415
21	25.649061349331404	26.05735121881346	27.02159570613662	21.27199172571852
22	23.640525863449078	25.12280820013313	25.944654156941716	25.292011779476077
23	23.62688635923189	26.46090149223549	26.25341250916322	23.658799639369402
24	23.56363908291976	24.519352297334873	27.753652648696924	24.163355971048443
25	22.607241260184864	26.275477961931564	25.92559044834472	25.191690329538847
26	22.579435629966042	26.511773156613106	26.31897692132692	24.58981429209393
27	23.35504419410015	26.6875068460832	26.578232825810368	23.379216134006285
28	21.323232025345256	27.06283019185885	27.374958923500813	24.238978859295084
29	22.940118890134055	25.85597104844078	25.76860449440096	25.43530556702421
30	23.950390121417918	26.94197049232817	25.19147968082507	23.91615970542884
31	24.69745578483498	27.8840442025261	23.437355179009277	23.981144833629646
32	24.328346576115806	27.523255618001198	23.40886494047067	24.739532865412322
33	23.22833899276211	28.83796690287409	23.443095356459754	24.490598747904045
34	22.229495454200755	27.05261372924057	25.816158441536558	24.901732375022117
35	22.59686681103125	26.8726670654949	24.726209334265807	25.804256789208047
36	23.159140890285723	30.59504048668279	23.585915184401884	22.659903438629602
37	23.806306401193115	28.862349491494005	24.374689293147174	22.956654814165706
38	23.287425956977106	29.17537348016953	23.992467201995265	23.5447333608581
39	23.500233820072296	27.420459045677063	24.6461628230298	24.433144311220836
40	23.80025025067197	26.629209814544875	26.45747845063658	23.113061484146577
41	21.676542580531002	27.844389582157213	24.423928429993005	26.055139407318777
42	23.289901079364007	26.913216942897343	25.115172184258643	24.68170979348
43	23.74458632805588	24.969297949966716	26.519935794272044	24.766179927705362
44	22.746164086922086	25.50002738433279	25.343620714351918	26.410187814393204
45	23.265202517673426	24.99289060590996	25.13750094791921	26.6044059284974
46	23.426980729855664	26.75233398774867	25.431197917105518	24.389487365290147
47	21.551996528509196	27.231612473774234	27.304970888347757	23.911420109368812
48	22.019373362206252	26.384646657847505	26.712047421238445	24.883932558707798
49	22.958550652589715	25.868609971267514	27.415772111795484	23.757067264347285
50	21.33887269234334	25.61483093334232	26.97030274433144	26.075993629982896
51	21.702452372325816	25.027068359720595	24.9304332622745	28.34004600567909
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	816.0
1	857.5
2	899.0
3	2195.5
4	3492.0
5	2784.0
6	2076.0
7	2051.5
8	2027.0
9	2175.5
10	2324.0
11	2448.5
12	2573.0
13	2929.0
14	3285.0
15	3452.0
16	3619.0
17	3724.0
18	3829.0
19	3945.5
20	4062.0
21	4583.5
22	5105.0
23	5610.5
24	6116.0
25	8992.0
26	13722.0
27	15576.0
28	18556.0
29	21536.0
30	24629.5
31	27723.0
32	30356.5
33	32990.0
34	36622.0
35	40254.0
36	42569.0
37	44884.0
38	50920.0
39	56956.0
40	61486.0
41	66016.0
42	76722.5
43	87429.0
44	96434.5
45	105440.0
46	123970.0
47	142500.0
48	167009.5
49	191519.0
50	193319.5
51	195120.0
52	176887.5
53	158655.0
54	142992.5
55	127330.0
56	118162.0
57	108994.0
58	100086.5
59	91179.0
60	88012.0
61	84845.0
62	78893.5
63	72942.0
64	64170.5
65	55399.0
66	47197.0
67	38995.0
68	33291.5
69	27588.0
70	22989.5
71	18391.0
72	16252.0
73	14113.0
74	11450.0
75	7411.5
76	6036.0
77	4545.5
78	3055.0
79	2273.0
80	1491.0
81	1165.0
82	839.0
83	484.5
84	130.0
85	96.5
86	63.0
87	42.0
88	21.0
89	14.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1898896.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.045085221524754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9734214527719	18.485088102006372
2	11.196505809773479	5.832278959976945
3	4.798589790054208	3.7493904007530126
4	2.6078603125705953	2.7168777634693333
5	1.5249033189969399	1.9858118448930622
6	0.9889159187306962	1.5453839628158055
7	0.6897909775660276	1.2575965357022238
8	0.4884533952480438	1.0177448244782732
9	0.39088457850985014	0.9162559943161946
>10	4.979422820952057	33.68327875341815
>50	1.2526809691443732	21.105948262067276
>100	0.09982808803722415	4.494344123858101
>500	0.005489519218725512	1.0003483878187378
>1k	0.0026431018460530238	1.1726597347014953
>5k	6.099465798583901E-4	1.0369923497249665
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	7095	0.37363815606541906	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	6848	0.3606305979895687	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGCT	5640	0.2970146864283247	Illumina Single End Adapter 1 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2711	0.14276716576368587	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC	2587	0.1362370556365383	Illumina Single End Adapter 1 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2131	0.11222310226573756	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5798653533421526E-4	0.0	0.0	0.3150778136348699	0.0
2	1.5798653533421526E-4	0.0	0.0	1.3379353055670242	0.0
3	1.5798653533421526E-4	0.0	0.0	1.773030223877453	0.0
4	1.5798653533421526E-4	0.0	0.0	2.1923264886544604	0.0
5	1.5798653533421526E-4	0.0	0.0	3.293861275183054	0.0
6	1.5798653533421526E-4	0.0	0.0	3.8429171476478965	0.0
7	1.5798653533421526E-4	0.0	0.0	4.532265063489523	0.0
8	1.5798653533421526E-4	0.0	0.0	5.765560620486851	0.0
9	2.1064871377895366E-4	0.0	0.0	6.19965495740683	0.0
10	2.1064871377895366E-4	0.0	0.0	7.409989804602253	0.0
11	2.1064871377895366E-4	0.0	0.0	9.140363663939468	0.0
12	2.1064871377895366E-4	0.0	0.0	10.394039483994911	0.0
13	2.1064871377895366E-4	0.0	0.0	10.91650095634516	0.0
14	2.1064871377895366E-4	0.0	0.0	11.10776998845645	0.0
15	2.1064871377895366E-4	0.0	0.0	11.510846302272478	0.0
16	2.1064871377895366E-4	0.0	0.0	12.341276194167559	0.0
17	2.1064871377895366E-4	0.0	0.0	13.343279463435596	0.0
18	2.1064871377895366E-4	0.0	0.0	14.462192768850953	0.0
19	2.1064871377895366E-4	0.0	0.0	15.152593928261474	0.0
20	2.1064871377895366E-4	0.0	0.0	15.79849554688619	0.0
21	4.212974275579073E-4	0.0	0.0	16.593747103580185	0.0
22	4.739596060026457E-4	0.0	0.0	17.400057717747575	0.0
23	4.739596060026457E-4	0.0	0.0	18.221271728414827	0.0
24	6.846083197815994E-4	0.0	0.0	18.893767747154136	0.0
25	7.899326766710763E-4	0.0	0.0	19.51075782981269	0.0
26	7.899326766710763E-4	0.0	0.0	20.076191639773846	0.0
27	7.899326766710763E-4	0.0	0.0	20.64736562718548	0.0
28	7.899326766710763E-4	0.0	0.0	21.194104363798754	0.0
29	8.425948551158146E-4	0.0	0.0	21.779813112461134	0.0
30	8.425948551158146E-4	0.0	0.0	22.459681836182707	0.0
31	8.425948551158146E-4	0.0	0.0	23.046654477127763	0.0
32	8.425948551158146E-4	0.0	0.0	23.635417632139937	0.0
33	8.425948551158146E-4	0.0	0.0	24.25003791676848	0.0
34	0.0016851897102316293	0.0	0.0	24.809362914030046	0.0
35	0.0017905140671211062	0.0	0.0	25.41655783149788	0.0
36	0.0017905140671211062	0.0	0.0	25.980253789570362	0.0
37	0.0017905140671211062	0.0	0.0	26.54495032903329	0.0
38	0.0017905140671211062	0.0	0.0	27.136451917324592	0.0
39	0.0018431762455658445	0.0	0.0	27.745226700145768	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCTCG	30	2.1662527E-6	45.000004	13
GTCGAAC	20	7.034451E-4	45.0	10
CGTAATA	20	7.034451E-4	45.0	34
TACGGGT	130	0.0	41.538464	4
ACGTAAG	80	0.0	39.375	1
GATCGAC	35	6.2504405E-6	38.57143	10
ATTCGAC	70	0.0	38.57143	42
TCTAGCG	70	0.0	38.57143	1
GCGTACG	135	0.0	38.333332	1
CGTTAGG	305	0.0	36.885242	2
CGTAAGG	385	0.0	36.818184	2
CGTATGG	240	0.0	36.562504	2
AACACGT	640	0.0	36.210938	41
CGCATTA	25	0.0021074535	36.000004	17
TCGCTAA	25	0.0021074535	36.000004	26
CGAATAT	200	0.0	36.000004	14
TTACGCG	25	0.0021074535	36.000004	1
CGGTCAT	25	0.0021074535	36.000004	34
GACCGAT	790	0.0	35.886074	9
AGGGTAC	395	0.0	35.886074	6
>>END_MODULE