FastQCFastQC Report
Sat 18 Jun 2016
SRR3552738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062517
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC52110.4904392117961407TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC37140.3495473484188959TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT32110.30220692939501204TruSeq Adapter, Index 20 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC16120.1517152196153097TruSeq Adapter, Index 23 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA15680.14757410940248486No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15150.14258595391885495No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.11359818242908112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCAA207.033047E-445.018
ACTCGAT207.033047E-445.032
GCCGTAT207.033047E-445.010
TATTCGG302.1653468E-644.9999962
CATACGA700.041.78571318
GCGTTAG850.039.7058831
CGTTAGG1650.039.5454522
GCCGATT403.4578989E-739.3759
CGATACG403.4578989E-739.37510
GCGTAAG750.039.01
GTATTCG356.247843E-638.5714261
CTACGCG356.247843E-638.5714261
TATCGCG356.247843E-638.5714261
CGCGAGG1000.038.252
ACGGGTA950.037.8947375
TGTAGCG1900.037.8947371
CACTAGG1250.037.82
ATCGAGG900.037.52
ACCGGAT301.1398638E-437.49999642
CACGTAA301.1398638E-437.49999623
Produced by FastQC (version 0.11.5)