##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552738_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1062517
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.604930556405215	33.0	33.0	33.0	27.0	33.0
2	31.80847271149544	33.0	33.0	33.0	27.0	33.0
3	31.849608994491383	33.0	33.0	33.0	27.0	33.0
4	31.755438265928923	33.0	33.0	33.0	27.0	33.0
5	31.822867775292067	33.0	33.0	33.0	27.0	33.0
6	35.23790113475832	37.0	37.0	37.0	33.0	37.0
7	35.487099971106346	37.0	37.0	37.0	33.0	37.0
8	35.554752535724134	37.0	37.0	37.0	33.0	37.0
9	35.43805228528108	37.0	37.0	37.0	33.0	37.0
10	35.50195526283344	37.0	37.0	37.0	33.0	37.0
11	35.469119082329975	37.0	37.0	37.0	33.0	37.0
12	35.477348597716556	37.0	37.0	37.0	33.0	37.0
13	35.52138365786148	37.0	37.0	37.0	33.0	37.0
14	35.578816150706295	37.0	37.0	37.0	33.0	37.0
15	35.62188746156532	37.0	37.0	37.0	33.0	37.0
16	35.65358295443743	37.0	37.0	37.0	33.0	37.0
17	35.66121295000456	37.0	37.0	37.0	33.0	37.0
18	35.636106528177905	37.0	37.0	37.0	33.0	37.0
19	35.6246845932818	37.0	37.0	37.0	33.0	37.0
20	35.60765333637015	37.0	37.0	37.0	33.0	37.0
21	35.52424949436103	37.0	37.0	37.0	33.0	37.0
22	35.570004056405686	37.0	37.0	37.0	33.0	37.0
23	35.59638575194562	37.0	37.0	37.0	33.0	37.0
24	35.597801258709275	37.0	37.0	37.0	33.0	37.0
25	35.59725350276749	37.0	37.0	37.0	33.0	37.0
26	35.553245736303516	37.0	37.0	37.0	33.0	37.0
27	35.54201485717405	37.0	37.0	37.0	33.0	37.0
28	35.51880769907682	37.0	37.0	37.0	33.0	37.0
29	35.50767564189561	37.0	37.0	37.0	33.0	37.0
30	35.50068469492724	37.0	37.0	37.0	33.0	37.0
31	35.490045806325924	37.0	37.0	37.0	33.0	37.0
32	35.46608854258332	37.0	37.0	37.0	33.0	37.0
33	35.44976315673067	37.0	37.0	37.0	33.0	37.0
34	35.42245818184556	37.0	37.0	37.0	33.0	37.0
35	35.40935627382903	37.0	37.0	37.0	33.0	37.0
36	35.40664572896245	37.0	37.0	37.0	33.0	37.0
37	35.40091029131769	37.0	37.0	37.0	33.0	37.0
38	35.36902750732459	37.0	37.0	37.0	33.0	37.0
39	35.37135970530354	37.0	37.0	37.0	33.0	37.0
40	35.368852451301954	37.0	37.0	37.0	33.0	37.0
41	35.36421346670218	37.0	37.0	37.0	33.0	37.0
42	35.335975800857774	37.0	37.0	37.0	33.0	37.0
43	35.314811904186	37.0	37.0	37.0	33.0	37.0
44	35.29118310577619	37.0	37.0	37.0	33.0	37.0
45	35.257694700414206	37.0	37.0	37.0	33.0	37.0
46	35.26226309790808	37.0	37.0	37.0	33.0	37.0
47	35.24303705258363	37.0	37.0	37.0	33.0	37.0
48	35.23877170906442	37.0	37.0	37.0	33.0	37.0
49	35.233341207717146	37.0	37.0	37.0	33.0	37.0
50	35.22263926130123	37.0	37.0	37.0	33.0	37.0
51	34.846560572677895	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	759.0
15	2972.0
16	2834.0
17	2275.0
18	2144.0
19	2166.0
20	2263.0
21	2575.0
22	2933.0
23	3370.0
24	4373.0
25	5382.0
26	7108.0
27	9068.0
28	11618.0
29	13952.0
30	17047.0
31	21073.0
32	27210.0
33	37106.0
34	56747.0
35	119365.0
36	708177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.380952963576114	19.285244377266434	24.135237365613914	13.198565293543538
2	29.6363258187869	24.357257342706045	25.85718628501944	20.149230553487616
3	32.43006935418445	24.371092415462527	25.54368541868036	17.65515281167266
4	30.360173060760438	26.6177388220612	24.265305872753096	18.756782244425267
5	25.916385337834598	30.258809976687434	23.74390245050197	20.080902234976005
6	27.026673455577654	35.885261129939565	21.90788476796136	15.180180646521421
7	80.69969704014147	7.023511153233312	7.181908618873862	5.094883187751349
8	81.99567630447324	5.546452433231656	7.126944792412733	5.330926469882364
9	76.7335487338085	7.605713602700003	9.460648629621925	6.200089033869576
10	42.7455748943311	25.45211041329221	16.27221023287157	15.530104459505118
11	32.68248884488437	26.249085896978592	21.73245228076351	19.33597297737354
12	29.812228886690757	23.136382759052328	27.90825935020334	19.143129004053584
13	26.337460953565923	24.12582575149386	28.63869472206092	20.898018572879305
14	21.41236328454039	27.592405580334244	28.04341012896735	22.951821006158017
15	21.47118587279074	25.585378869232212	32.09134536200362	20.85208989597343
16	23.984745655834214	24.890237050324842	29.679148663033157	21.44586863080779
17	23.657033252173846	24.36431605329609	27.61151115699796	24.367139537532108
18	24.590853605165847	24.965906427850097	28.80951551833994	21.633724448644116
19	25.261148762796264	26.9178751963498	26.102170600564506	21.718805440289426
20	27.655651627221022	26.005607439692728	26.029512939557677	20.309227993528577
21	25.80890470458355	26.792136031705844	26.387718972967022	21.011240290743583
22	22.693095733997666	26.466023602445894	26.193086792964255	24.64779387059219
23	23.367908466405716	26.30254386518051	26.883711037093995	23.44583663131978
24	24.2827173588752	24.81343827910518	27.20803525967114	23.69580910234848
25	23.10683029071535	25.385193836898612	26.256897536698236	25.251078335687804
26	22.82994060330329	26.437224063238517	27.215282202543584	23.51755313091461
27	23.507765052229754	26.483435088567997	26.912134111736563	23.09666574746569
28	20.629599338175293	27.991458019024634	27.216129247814386	24.16281339498568
29	23.64451580539417	25.722882551526233	26.345649057850366	24.286952585229223
30	24.9164954537198	24.493725747446867	26.151487458553603	24.438291340279733
31	24.220694821824026	27.17481226182734	25.25663118801864	23.347861728329995
32	25.398087748243086	26.763242376357272	25.264160479314686	22.574509396084956
33	24.735604230332314	27.00728553049034	24.702569464770914	23.554540774406433
34	22.816481995111605	25.986595979170218	27.691039296312436	23.50588272940574
35	23.43746029475293	25.416628628059595	26.463482466633476	24.682428610554
36	23.40734312956875	29.03944125129292	25.195079231673468	22.358136387464857
37	22.919256821302625	28.662882570349463	26.482023346449985	21.935837261897927
38	21.471562337355543	28.69911728471168	25.309900923938155	24.51941945399462
39	23.026549222271267	25.34058278596954	26.392424780027053	25.240443211732146
40	23.549740851205204	23.326309131995064	29.575244443147735	23.548705573651997
41	20.830913764203302	25.930032178308675	27.80689626613033	25.43215779135769
42	22.739683223891944	26.645785432138968	26.47731753938996	24.137213804579126
43	23.14551202474878	25.0666106989347	26.615009453966383	25.17286782235014
44	22.06336463322469	25.071881202841933	26.276756042491556	26.58799812144182
45	23.220899053850435	22.99709087007549	26.078547449123167	27.70346262695091
46	22.28801986227044	25.028587777889673	27.617628706175996	25.065763653663893
47	20.93208861599391	25.226137558269656	29.91330962234016	23.928464203396274
48	21.42102196953084	24.13627264316712	29.190591774061026	25.25211361324101
49	22.260632065181074	23.78926643056064	29.262120041373457	24.687981462884828
50	20.652563676628233	23.836418617302122	29.12480459136183	26.386213114707814
51	20.27214623389555	22.700248560728912	27.750708929833596	29.27689627554194
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	470.0
1	502.0
2	534.0
3	1386.0
4	2238.0
5	1743.0
6	1248.0
7	1221.0
8	1194.0
9	1286.5
10	1379.0
11	1411.0
12	1443.0
13	1703.0
14	1963.0
15	2008.0
16	2053.0
17	2205.5
18	2358.0
19	2322.0
20	2286.0
21	2496.5
22	2707.0
23	3189.0
24	3671.0
25	5292.0
26	8325.0
27	9737.0
28	10845.0
29	11953.0
30	13587.0
31	15221.0
32	17156.0
33	19091.0
34	21533.5
35	23976.0
36	25894.5
37	27813.0
38	30146.5
39	32480.0
40	35955.5
41	39431.0
42	44054.5
43	48678.0
44	54009.5
45	59341.0
46	70111.0
47	80881.0
48	92121.5
49	103362.0
50	105731.5
51	108101.0
52	96331.5
53	84562.0
54	77578.5
55	70595.0
56	64756.5
57	58918.0
58	56428.0
59	53938.0
60	52750.5
61	51563.0
62	47503.5
63	43444.0
64	37605.5
65	31767.0
66	25398.0
67	19029.0
68	16088.0
69	13147.0
70	11318.5
71	9490.0
72	7698.0
73	5906.0
74	4993.0
75	3505.5
76	2931.0
77	2196.5
78	1462.0
79	1106.0
80	750.0
81	507.5
82	265.0
83	182.0
84	99.0
85	70.5
86	42.0
87	24.0
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1062517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.558547197239243
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.22099958157179	19.76851183590396
2	12.043437186849403	6.878861386352497
3	4.668656862203336	3.999901720408465
4	2.3282749881835327	2.6596860455276423
5	1.388750571028101	1.9830349363949487
6	0.8881529286439762	1.521861439862715
7	0.6896308250589388	1.378639812628172
8	0.5401573450785726	1.2340887226689488
9	0.47312746457413973	1.2160649724615638
>10	7.413075071981573	49.05746290127871
>50	0.27837305469799883	4.939516977534836
>100	0.06241087600152391	3.102822148889171
>500	0.0026417302011061557	0.558474037008479
>1k	0.001981297650829617	1.2096498603018844
>5k	3.3021627513826946E-4	0.4914232027779777
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	5211	0.4904392117961407	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	3714	0.3495473484188959	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGCT	3211	0.30220692939501204	TruSeq Adapter, Index 20 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC	1612	0.1517152196153097	TruSeq Adapter, Index 23 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1568	0.14757410940248486	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1515	0.14258595391885495	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.11359818242908112	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.30983033683225775	0.0
2	0.0	0.0	0.0	1.4197419900105128	0.0
3	0.0	0.0	0.0	1.8654760347363855	0.0
4	0.0	0.0	0.0	2.3128100538626675	0.0
5	0.0	0.0	0.0	3.4685562678055977	0.0
6	0.0	0.0	0.0	4.093675677659745	0.0
7	0.0	0.0	0.0	4.825428675494133	0.0
8	0.0	0.0	0.0	6.068891133035989	0.0
9	0.0	0.0	0.0	6.49768427234576	0.0
10	0.0	0.0	0.0	7.689476968368506	0.0
11	0.0	0.0	0.0	9.469495546894779	0.0
12	0.0	0.0	0.0	10.76208663014333	0.0
13	0.0	0.0	0.0	11.270125560343976	0.0
14	0.0	0.0	0.0	11.466263598605952	0.0
15	0.0	0.0	0.0	11.875480580546006	0.0
16	0.0	0.0	0.0	12.738902059919983	0.0
17	0.0	0.0	0.0	13.767026786394947	0.0
18	0.0	0.0	0.0	14.940749183307185	0.0
19	0.0	0.0	0.0	15.61189138620841	0.0
20	0.0	0.0	0.0	16.295739268171708	0.0
21	1.8823228240112864E-4	0.0	0.0	17.112008560804203	0.0
22	1.8823228240112864E-4	0.0	0.0	18.026440988708888	0.0
23	1.8823228240112864E-4	0.0	0.0	18.92609718244508	0.0
24	1.8823228240112864E-4	0.0	0.0	19.652579676372238	0.0
25	0.001129393694406772	0.0	0.0	20.281275499592006	0.0
26	0.001129393694406772	0.0	0.0	20.873171911602356	0.0
27	0.001129393694406772	0.0	0.0	21.433539416310516	0.0
28	0.001129393694406772	0.0	0.0	21.990706972217858	0.0
29	0.001129393694406772	0.0	0.0	22.585709216887825	0.0
30	0.001129393694406772	0.0	0.0	23.268239472874317	0.0
31	0.001129393694406772	0.0	0.0	23.908699813744157	0.0
32	0.001129393694406772	0.0	0.0	24.497678625377286	0.0
33	0.001129393694406772	0.0	0.0	25.07724582289036	0.0
34	0.001129393694406772	0.0	0.0	25.66537758925269	0.0
35	0.001129393694406772	0.0	0.0	26.28757939872962	0.0
36	0.001129393694406772	0.0	0.0	26.90996944048895	0.0
37	0.0012235098356073362	0.0	0.0	27.569723590304907	0.0
38	0.004611690918827652	0.0	0.0	28.198231181242278	0.0
39	0.004611690918827652	0.0	0.0	28.84951487835018	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCAA	20	7.033047E-4	45.0	18
ACTCGAT	20	7.033047E-4	45.0	32
GCCGTAT	20	7.033047E-4	45.0	10
TATTCGG	30	2.1653468E-6	44.999996	2
CATACGA	70	0.0	41.785713	18
GCGTTAG	85	0.0	39.705883	1
CGTTAGG	165	0.0	39.545452	2
GCCGATT	40	3.4578989E-7	39.375	9
CGATACG	40	3.4578989E-7	39.375	10
GCGTAAG	75	0.0	39.0	1
GTATTCG	35	6.247843E-6	38.571426	1
CTACGCG	35	6.247843E-6	38.571426	1
TATCGCG	35	6.247843E-6	38.571426	1
CGCGAGG	100	0.0	38.25	2
ACGGGTA	95	0.0	37.894737	5
TGTAGCG	190	0.0	37.894737	1
CACTAGG	125	0.0	37.8	2
ATCGAGG	90	0.0	37.5	2
ACCGGAT	30	1.1398638E-4	37.499996	42
CACGTAA	30	1.1398638E-4	37.499996	23
>>END_MODULE