SRR3552736_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552736_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907523
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	8126	0.8954043038027687	RNA PCR Primer, Index 38 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	6460	0.7118276892155901	RNA PCR Primer, Index 27 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGCT	5704	0.6285240153693075	RNA PCR Primer, Index 38 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	2639	0.2907915281485979	RNA PCR Primer, Index 27 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC	1810	0.19944398103408947	RNA PCR Primer, Index 27 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1338	0.14743427990254793	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1320	0.14545085909668407	No Hit
GCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	1261	0.13894964645524135	RNA PCR Primer, Index 27 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCC	1216	0.13399109444058166	No Hit
ACCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	1108	0.12209056960539844	RNA PCR Primer, Index 27 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1089	0.11999695875476435	No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG	1028	0.11327536602378122	RNA PCR Primer, Index 38 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	20	7.0325006E-4	45.000004	1
GCGATAT	40	6.8121153E-9	45.000004	9
CCGCGAT	20	7.0325006E-4	45.000004	44
ATCGAGT	20	7.0325006E-4	45.000004	18
ACGTTAG	25	3.8901548E-5	45.0	1
CCGTACA	35	1.2115561E-7	45.0	17
GTTGCAC	45	3.8380676E-10	45.0	8
GATAACG	30	2.1649957E-6	44.999996	1
GTATGCG	65	0.0	41.538464	1
TCTAGCG	60	3.6379788E-12	41.249996	1
AGTTACG	60	3.6379788E-12	41.249996	1
TCATCGA	55	6.002665E-11	40.909092	16
TCGTGAG	100	0.0	40.5	1
GCTACGA	50	1.0804797E-9	40.5	10
CGACAGG	90	0.0	40.0	2
TACGTAG	85	0.0	39.705883	1
GCGATCA	75	0.0	39.0	9
TGCGTAG	75	0.0	39.0	1
TAGGGTA	180	0.0	38.75	5
TGACCGG	35	6.246837E-6	38.571426	2