FastQCFastQC Report
Sat 18 Jun 2016
SRR3552735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3126337
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC114730.3669789917081876Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT75920.2428401032902083Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC72170.23084523517458289Illumina PCR Primer Index 5 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC53680.17170253878580588Illumina PCR Primer Index 5 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC35270.11281573291682886Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG207.035151E-445.022
TCGAACG207.035151E-445.01
TATCGCG900.042.51
GCGTAAG3400.042.352941
CAATGCG403.460773E-739.3751
TATTACG750.039.01
CGAATAT3000.038.2514
CGTAAGG4650.038.2258032
ACGTACG301.1403714E-437.4999961
TAAGGGA22200.036.3851364
CACAACG7200.036.2512
TACGGGA8550.036.052634
ACTATCG250.002107661736.044
GTCGACG504.8836228E-836.013
ACGTATC250.002107661736.010
AACGGGA11750.035.808514
ACAACGA7400.035.2702713
GGCCGAT4150.035.2409638
TTACACG17250.035.2173934
CGTTTAG458.706302E-735.018
Produced by FastQC (version 0.11.5)