##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552735_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3126337
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.684185677999526	31.0	31.0	34.0	30.0	34.0
2	31.851657386903586	31.0	31.0	34.0	30.0	34.0
3	31.896157068159958	33.0	31.0	34.0	30.0	34.0
4	35.51568368989012	37.0	35.0	37.0	33.0	37.0
5	35.398832243612894	37.0	35.0	37.0	33.0	37.0
6	35.36902707545604	37.0	35.0	37.0	33.0	37.0
7	35.79647843466651	37.0	35.0	37.0	35.0	37.0
8	35.78867537312836	37.0	35.0	37.0	35.0	37.0
9	37.36602260089043	39.0	37.0	39.0	35.0	39.0
10	36.77514516189394	39.0	37.0	39.0	32.0	39.0
11	36.58771399244547	39.0	35.0	39.0	32.0	39.0
12	36.37596010922687	38.0	35.0	39.0	32.0	39.0
13	36.29577137717399	38.0	35.0	39.0	32.0	39.0
14	37.27529885613739	39.0	36.0	41.0	32.0	41.0
15	37.364268151514054	39.0	36.0	41.0	32.0	41.0
16	37.429110809231375	39.0	36.0	41.0	32.0	41.0
17	37.42213907201943	39.0	36.0	41.0	32.0	41.0
18	37.366813622459766	39.0	36.0	41.0	32.0	41.0
19	37.34459880684648	39.0	36.0	41.0	32.0	41.0
20	37.29843327830621	39.0	35.0	41.0	32.0	41.0
21	37.232932981953	39.0	35.0	41.0	32.0	41.0
22	37.181085084557424	39.0	35.0	41.0	32.0	41.0
23	37.129914017586714	39.0	35.0	40.0	32.0	41.0
24	37.126349462645905	39.0	35.0	41.0	32.0	41.0
25	37.07846211077053	39.0	35.0	41.0	31.0	41.0
26	36.99193273150016	39.0	35.0	40.0	31.0	41.0
27	36.93464364206418	39.0	35.0	40.0	31.0	41.0
28	36.83714135744163	39.0	35.0	40.0	31.0	41.0
29	36.8246404658231	39.0	35.0	40.0	31.0	41.0
30	36.72076042985769	39.0	35.0	40.0	31.0	41.0
31	36.57042570906464	39.0	35.0	40.0	30.0	41.0
32	36.48057391125781	38.0	35.0	40.0	30.0	41.0
33	36.388975340790196	38.0	35.0	40.0	30.0	41.0
34	36.222323761002094	38.0	35.0	40.0	30.0	41.0
35	36.18876723782497	38.0	35.0	40.0	30.0	41.0
36	36.121172157704045	38.0	35.0	40.0	30.0	41.0
37	36.0767111798888	38.0	35.0	40.0	30.0	41.0
38	36.03247922408877	38.0	35.0	40.0	29.0	41.0
39	35.95070205163423	38.0	35.0	40.0	29.0	41.0
40	35.82714659360139	38.0	34.0	40.0	29.0	41.0
41	35.70991163140762	38.0	34.0	40.0	29.0	41.0
42	35.66027686714516	38.0	34.0	40.0	28.0	41.0
43	35.61880916868527	38.0	34.0	40.0	28.0	41.0
44	35.46971743609214	38.0	34.0	40.0	28.0	41.0
45	35.39918217389872	38.0	34.0	40.0	28.0	41.0
46	35.27839225265862	38.0	34.0	40.0	27.0	41.0
47	35.15281110129842	37.0	34.0	40.0	27.0	41.0
48	35.08430377147441	37.0	34.0	40.0	27.0	41.0
49	35.03522556909252	37.0	34.0	40.0	27.0	41.0
50	34.918512303695984	37.0	34.0	40.0	26.0	41.0
51	33.34607817391407	35.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	5.0
10	7.0
11	15.0
12	16.0
13	28.0
14	65.0
15	154.0
16	294.0
17	625.0
18	1329.0
19	2221.0
20	3721.0
21	6003.0
22	8747.0
23	12566.0
24	17328.0
25	22876.0
26	29496.0
27	37327.0
28	46751.0
29	58342.0
30	73006.0
31	92066.0
32	118000.0
33	158054.0
34	248707.0
35	271738.0
36	283854.0
37	392029.0
38	591086.0
39	649777.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.856384324530595	20.779333769839912	26.405726573942605	9.958555331686892
2	30.913078148644885	23.998340549979098	26.98669401283355	18.10188728854247
3	32.36100266861826	24.534719065794892	26.2923670736712	16.81191119191565
4	28.350526510737645	26.98515866971475	25.638726727157053	19.025588092390553
5	25.68590654174518	30.299932476889087	24.704598384627122	19.309562596738612
6	26.427893090220277	35.93604912074418	22.918258652218235	14.717799136817304
7	82.26032574223444	6.088339164971659	7.222669852930122	4.4286652398637765
8	83.50209846219393	4.874138648520617	6.816379680117659	4.8073832091677895
9	77.67588714844241	7.317477290516025	9.056125427297184	5.950510133744379
10	43.23513428014958	25.34032639475527	15.944090480328896	15.480448844766256
11	32.79716805961737	25.603893630149276	21.781528990636644	19.817409319596706
12	29.284462935377725	22.975322238133636	27.777747568480297	19.962467258008335
13	26.44267076773873	23.92416428555207	29.24387230167445	20.389292645034747
14	21.281678846522304	26.76957090678324	28.731963316814536	23.21678692987992
15	20.71977525135646	25.62634802326173	32.9368522971132	20.71702442826861
16	24.087006615089802	25.022574341793607	29.659470492144642	21.230948550971952
17	24.204460363678002	24.8598279712008	26.996257921011075	23.93945374411012
18	24.367750501625384	25.367194899334265	29.119605467996575	21.145449131043776
19	25.394831075472666	27.001727580871798	26.11263596982667	21.490805373828863
20	26.56751335508616	27.29587373338191	25.683315650232203	20.453297261299724
21	25.611858222578054	27.217603220638082	26.878580268218045	20.29195828856582
22	23.49058978606593	26.236998762449478	25.944675829892937	24.32773562159166
23	23.03654404499579	26.87074362104917	27.318807921218983	22.773904412736055
24	24.381696534954486	24.560915857759415	27.555442679404045	23.501944927882054
25	23.582230578469307	26.141711530138945	25.419940332728046	24.8561175586637
26	22.6746828636836	26.45175488119163	26.94373639182212	23.929825863302646
27	22.991731217715813	26.119545013861266	27.17544525750103	23.71327851092189
28	20.137592332496464	27.573738851569747	27.223776579428254	25.064892236505532
29	23.47785923270588	26.20424477591507	26.538213890569057	23.77968210080999
30	25.69601421727728	25.359326265850417	25.406442107808598	23.538217409063705
31	24.34833480843556	28.089454207911686	24.447332453283188	23.114878530369566
32	25.57606553612103	26.65064578770619	25.739291701438454	22.03399697473433
33	25.023405985982954	25.62756350323078	25.721379365052456	23.627651145733807
34	22.703982328200702	26.85980430132772	27.803848401499902	22.632364968971675
35	23.008364101502814	25.196100100532988	28.09968982870369	23.695845969260514
36	23.069585908364964	29.110169505078947	25.864230247730813	21.956014338825277
37	21.75322110188377	29.834179744538098	27.188079851916157	21.224519301661974
38	22.506626764804945	28.129021279535767	27.54124075555514	21.823111200104147
39	24.734409630183823	25.121444041381334	27.784912503034704	22.35923382540014
40	25.24862162972194	24.541660096144465	28.570336467245856	21.63938180688774
41	20.910029852827765	25.898487591069035	28.00350697957386	25.187975576529336
42	22.017619981467128	25.216283465282213	28.93673970528449	23.829356847966167
43	22.839284440544958	23.357334797880075	29.493685421629213	24.30969533994576
44	22.59836351615325	24.775639990186598	27.444162289606016	25.181834204054137
45	23.746736196385736	23.46896703714283	26.850912105764667	25.933384660706764
46	23.075407417690418	25.555914157686775	27.35610396447984	24.01257446014297
47	20.867647985485892	25.503552560072702	30.10986979330763	23.518929661133782
48	21.297895908214628	23.84295103183054	30.405199439471815	24.453953620483013
49	22.81871724001603	23.322277796667475	30.450204184641645	23.408800778674852
50	20.782372469762535	23.349402191766274	29.665323987785065	26.20290135068612
51	20.512312012428602	22.977081485457262	27.924500781585603	28.586105720528533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1132.0
1	1611.0
2	2090.0
3	3489.5
4	4889.0
5	3823.5
6	2758.0
7	2833.0
8	2908.0
9	3374.0
10	3840.0
11	4308.5
12	4777.0
13	4811.0
14	4845.0
15	5266.5
16	5688.0
17	6137.0
18	6586.0
19	6831.0
20	7076.0
21	7736.0
22	8396.0
23	9649.5
24	10903.0
25	14444.5
26	21328.0
27	24670.0
28	30827.5
29	36985.0
30	41472.0
31	45959.0
32	50070.5
33	54182.0
34	61520.0
35	68858.0
36	75837.5
37	82817.0
38	93081.5
39	103346.0
40	118642.0
41	133938.0
42	152612.5
43	171287.0
44	205599.5
45	239912.0
46	277806.0
47	315700.0
48	313347.0
49	310994.0
50	276001.0
51	241008.0
52	223746.0
53	206484.0
54	192819.0
55	179154.0
56	172458.0
57	165762.0
58	160076.5
59	154391.0
60	145216.5
61	136042.0
62	127553.5
63	119065.0
64	101822.5
65	84580.0
66	71041.5
67	57503.0
68	47267.5
69	37032.0
70	30834.5
71	24637.0
72	21105.5
73	17574.0
74	15611.5
75	10689.5
76	7730.0
77	5994.0
78	4258.0
79	3532.0
80	2806.0
81	2013.5
82	1221.0
83	857.0
84	493.0
85	370.5
86	248.0
87	158.5
88	69.0
89	49.5
90	30.0
91	36.0
92	42.0
93	30.0
94	18.0
95	18.5
96	19.0
97	9.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3126337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.983426639441923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.64726994910151	14.431519430057133
2	12.945521012745074	5.691738229860704
3	5.847005157510911	3.8561162682173897
4	3.2101677495897882	2.8228194889363785
5	1.9386816397678093	2.13094328025343
6	1.3194738610447132	1.7403934096162583
7	0.9405038843532187	1.4472848702052405
8	0.6931459279240277	1.2190178125556608
9	0.5315888823524936	1.051753067778507
>10	4.815155483403239	25.605410909009187
>50	1.8030457186734425	27.375503554580515
>100	0.29556068074256703	9.148965886172409
>500	0.009806403829603986	1.459626371053903
>1k	0.0024881920164666815	1.0005409936137057
>5k	4.3909270878823793E-4	0.6492126198914246
>10k+	1.4636423626274598E-4	0.369153808198162
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	11473	0.3669789917081876	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	7592	0.2428401032902083	Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	7217	0.23084523517458289	Illumina PCR Primer Index 5 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	5368	0.17170253878580588	Illumina PCR Primer Index 5 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	3527	0.11281573291682886	Illumina PCR Primer Index 5 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2794525990000437E-4	0.0	0.0	0.2551868208705587	0.0
2	1.5993157487500547E-4	0.0	0.0	1.1880676971164656	0.0
3	1.5993157487500547E-4	0.0	0.0	1.6290950079917808	0.0
4	1.5993157487500547E-4	0.0	0.0	2.1230596701507225	0.0
5	1.5993157487500547E-4	0.0	0.0	3.4596718140110934	0.0
6	1.5993157487500547E-4	0.0	0.0	4.264607430356996	0.0
7	1.5993157487500547E-4	0.0	0.0	5.116530943401175	0.0
8	1.5993157487500547E-4	0.0	0.0	6.540273809253449	0.0
9	1.5993157487500547E-4	0.0	0.0	7.025282303219391	0.0
10	1.5993157487500547E-4	0.0	0.0	8.31071634311976	0.0
11	1.5993157487500547E-4	0.0	0.0	10.2126546178483	0.0
12	1.5993157487500547E-4	0.0	0.0	11.585955064984997	0.0
13	1.5993157487500547E-4	0.0	0.0	12.124796527053865	0.0
14	1.5993157487500547E-4	0.0	0.0	12.329061134484222	0.0
15	1.5993157487500547E-4	0.0	0.0	12.72098945187291	0.0
16	1.5993157487500547E-4	0.0	0.0	13.61564668172369	0.0
17	1.5993157487500547E-4	0.0	0.0	14.685812821842303	0.0
18	1.5993157487500547E-4	0.0	0.0	15.87452024525827	0.0
19	1.5993157487500547E-4	0.0	0.0	16.57431684428134	0.0
20	1.5993157487500547E-4	0.0	0.0	17.25066747442774	0.0
21	4.797947246250164E-4	0.0	0.0	18.10508592003997	0.0
22	5.117810396000175E-4	0.0	0.0	19.00857137282385	0.0
23	5.757536695500197E-4	0.0	0.0	19.89753503860908	0.0
24	6.077399845250208E-4	0.0	0.0	20.600082460720007	0.0
25	6.397262995000219E-4	0.0	0.0	21.236482183462627	0.0
26	6.71712614475023E-4	0.0	0.0	21.814378936115972	0.0
27	7.036989294500241E-4	0.0	0.0	22.381336369047865	0.0
28	7.036989294500241E-4	0.0	0.0	22.947654075680262	0.0
29	7.036989294500241E-4	0.0	0.0	23.571483176637706	0.0
30	7.036989294500241E-4	0.0	0.0	24.287496837353107	0.0
31	7.676715594000263E-4	0.0	0.0	24.918426900235005	0.0
32	7.996578743750274E-4	0.0	0.0	25.5429597001219	0.0
33	7.996578743750274E-4	0.0	0.0	26.150923588851747	0.0
34	7.996578743750274E-4	0.0	0.0	26.782845227497866	0.0
35	8.316441893500285E-4	0.0	0.0	27.444482152755764	0.0
36	8.316441893500285E-4	0.0	0.0	28.08692728902866	0.0
37	8.316441893500285E-4	0.0	0.0	28.72732530114316	0.0
38	8.316441893500285E-4	0.0	0.0	29.37581585094633	0.0
39	8.316441893500285E-4	0.0	0.0	30.152091729074634	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATATCG	20	7.035151E-4	45.0	22
TCGAACG	20	7.035151E-4	45.0	1
TATCGCG	90	0.0	42.5	1
GCGTAAG	340	0.0	42.35294	1
CAATGCG	40	3.460773E-7	39.375	1
TATTACG	75	0.0	39.0	1
CGAATAT	300	0.0	38.25	14
CGTAAGG	465	0.0	38.225803	2
ACGTACG	30	1.1403714E-4	37.499996	1
TAAGGGA	2220	0.0	36.385136	4
CACAACG	720	0.0	36.25	12
TACGGGA	855	0.0	36.05263	4
ACTATCG	25	0.0021076617	36.0	44
GTCGACG	50	4.8836228E-8	36.0	13
ACGTATC	25	0.0021076617	36.0	10
AACGGGA	1175	0.0	35.80851	4
ACAACGA	740	0.0	35.27027	13
GGCCGAT	415	0.0	35.240963	8
TTACACG	1725	0.0	35.21739	34
CGTTTAG	45	8.706302E-7	35.0	18
>>END_MODULE