FastQCFastQC Report
Sat 18 Jun 2016
SRR3552734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182404
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC82410.37761111141658465Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC77050.3530510391293271Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT61470.28166187378688823Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC31740.1454359504473049Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.11157420899155243No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA23660.10841255789487189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG850.045.0000041
GCGTAAG3050.040.5737721
CGAATAT1500.040.514
CGTAAGG4400.038.863642
GCCGATC1050.038.571439
ACGTAAG659.094947E-1238.0769271
GGACGTA301.14025825E-437.4999968
CAATGCG301.14025825E-437.4999961
TAGGGCG7300.036.3698655
GACGTAA250.002107522736.018
CGTAGTA250.002107522736.039
GCGATCG250.002107522736.09
ACGTAGG3900.035.7692342
TACGAAT1700.035.73529412
AACGGGA6800.035.4044154
TCGTTAC702.1827873E-1135.35714324
TCGGTTA702.1827873E-1135.35714313
TATGCGG3000.035.252
GGCCGAT3200.035.1562548
CACAACG4800.035.1562512
Produced by FastQC (version 0.11.5)