##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552734_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2182404
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.353683369348662	33.0	33.0	33.0	27.0	33.0
2	31.56797641499924	33.0	33.0	33.0	27.0	33.0
3	31.633032655731935	33.0	33.0	33.0	27.0	33.0
4	31.54583156922366	33.0	33.0	33.0	27.0	33.0
5	31.64170978425626	33.0	33.0	33.0	27.0	33.0
6	34.98621932511121	37.0	37.0	37.0	27.0	37.0
7	35.28320879177274	37.0	37.0	37.0	33.0	37.0
8	35.373720447726456	37.0	37.0	37.0	33.0	37.0
9	35.248419632661964	37.0	37.0	37.0	33.0	37.0
10	35.301350254123435	37.0	37.0	37.0	33.0	37.0
11	35.27572026077665	37.0	37.0	37.0	33.0	37.0
12	35.280922322356446	37.0	37.0	37.0	33.0	37.0
13	35.33646382612935	37.0	37.0	37.0	33.0	37.0
14	35.396367491995065	37.0	37.0	37.0	33.0	37.0
15	35.43439299048205	37.0	37.0	37.0	33.0	37.0
16	35.46801829542101	37.0	37.0	37.0	33.0	37.0
17	35.47502845485987	37.0	37.0	37.0	33.0	37.0
18	35.459581727306215	37.0	37.0	37.0	33.0	37.0
19	35.45417255466907	37.0	37.0	37.0	33.0	37.0
20	35.42399482405641	37.0	37.0	37.0	33.0	37.0
21	35.360214240809675	37.0	37.0	37.0	33.0	37.0
22	35.38930326374035	37.0	37.0	37.0	33.0	37.0
23	35.4202613265005	37.0	37.0	37.0	33.0	37.0
24	35.41412497411112	37.0	37.0	37.0	33.0	37.0
25	35.40889083781005	37.0	37.0	37.0	33.0	37.0
26	35.36989072600674	37.0	37.0	37.0	33.0	37.0
27	35.3644824697902	37.0	37.0	37.0	33.0	37.0
28	35.33719787903615	37.0	37.0	37.0	33.0	37.0
29	35.33261485957687	37.0	37.0	37.0	33.0	37.0
30	35.31043427339759	37.0	37.0	37.0	33.0	37.0
31	35.28848050131873	37.0	37.0	37.0	33.0	37.0
32	35.27194140040066	37.0	37.0	37.0	33.0	37.0
33	35.251300400842375	37.0	37.0	37.0	33.0	37.0
34	35.214296711333006	37.0	37.0	37.0	33.0	37.0
35	35.19476916281312	37.0	37.0	37.0	33.0	37.0
36	35.18413364345007	37.0	37.0	37.0	33.0	37.0
37	35.17417994101917	37.0	37.0	37.0	33.0	37.0
38	35.15295426511315	37.0	37.0	37.0	33.0	37.0
39	35.143728658855096	37.0	37.0	37.0	33.0	37.0
40	35.121432603679246	37.0	37.0	37.0	33.0	37.0
41	35.120598202715904	37.0	37.0	37.0	33.0	37.0
42	35.108906050392136	37.0	37.0	37.0	33.0	37.0
43	35.109515011885975	37.0	37.0	37.0	33.0	37.0
44	35.068350314607194	37.0	37.0	37.0	33.0	37.0
45	35.01941528699544	37.0	37.0	37.0	33.0	37.0
46	35.04038711439312	37.0	37.0	37.0	33.0	37.0
47	35.01452205916045	37.0	37.0	37.0	27.0	37.0
48	35.00911517757482	37.0	37.0	37.0	27.0	37.0
49	35.00248487447787	37.0	37.0	37.0	27.0	37.0
50	34.98823178476579	37.0	37.0	37.0	27.0	37.0
51	34.58597949783817	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1279.0
15	5542.0
16	6667.0
17	6253.0
18	5832.0
19	5974.0
20	6117.0
21	6629.0
22	7695.0
23	8885.0
24	10764.0
25	13474.0
26	17094.0
27	22240.0
28	28068.0
29	32875.0
30	39002.0
31	47352.0
32	60571.0
33	81595.0
34	122883.0
35	254165.0
36	1391448.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.3070577216684	19.20602234966578	23.564198012833554	12.922721915832266
2	30.027758380208248	23.5385840568474	26.528910320912168	19.90474724203218
3	32.54466175831789	24.566624694602833	25.42989290708778	17.458820639991497
4	29.000038489665524	26.78285047131512	24.756048834221346	19.46106220479801
5	25.760674925449184	29.965487599912755	24.732405182541818	19.54143229209624
6	26.268509405224698	36.09588325534594	22.591555000815614	15.044052338613747
7	80.70948367030118	6.9064206260619025	7.657381493069111	4.7267142105677955
8	82.31060793510275	5.5873706243207035	7.016849309293788	5.08517213128275
9	76.7691958042599	7.80222177012139	9.363390096425777	6.065192329192945
10	42.91551884985548	25.425814835383363	16.156082925067953	15.5025833896932
11	32.751177142270635	25.843427706327514	21.68883488116774	19.71656027023411
12	29.252329082974555	23.132197338347986	27.7398685119712	19.875605066706257
13	26.771532676809613	23.826523411797265	29.004758055795353	20.397185855597773
14	21.592427433234178	26.561259968365157	28.619357369213034	23.22695522918763
15	20.731679377420495	25.503985513223032	32.80950731395287	20.954827795403602
16	24.17105173927467	24.654234504702156	29.731387955667238	21.44332580035594
17	24.44803986796212	24.470721278003523	27.160965614065958	23.920273239968402
18	24.444328364500798	24.950009255848137	29.475935711261524	21.129726668389537
19	25.467695257156787	26.597275298249084	26.574456425116523	21.360573019477602
20	26.70550457202241	26.775473285422862	26.171231357713786	20.347790784840935
21	25.671736305468652	27.038623462933536	27.02061579799157	20.269024433606244
22	23.44854573213759	26.17311001995964	26.230065560730274	24.148278687172496
23	22.850581285591485	26.759298461696368	27.363952778678925	23.026167474033223
24	24.04275285419198	24.509714974862582	27.79407479091864	23.653457380026797
25	23.279832698253852	25.99413307526929	25.810436564449112	24.915597662027743
26	22.492306648998078	26.154048471318784	27.30910500530608	24.04453987437706
27	22.646677700370784	25.925630634841212	27.686257906418792	23.74143375836921
28	20.146361535261114	27.254119768842067	27.7642911211673	24.83522757472952
29	23.266040568107464	26.022404651017865	27.112853532159946	23.598701248714722
30	25.514845097424676	25.204499258615726	25.980478408214065	23.300177235745537
31	24.116341428992982	27.951561672357638	24.883568761787462	23.048528136861922
32	25.349797745971873	26.466502077525515	26.070791659106195	22.112908517396413
33	24.741706851710315	25.54806534445501	26.013377908031693	23.696849895802977
34	22.67197090914423	26.518371483923232	27.970531578937724	22.839126027994816
35	22.989327365602335	25.150292979668293	27.95815990073332	23.90221975399605
36	23.241663779941753	28.861200767593903	25.863314033515334	22.033821418949014
37	21.708583745264395	29.883101387277517	27.122430127510768	21.285884739947324
38	22.390217393296567	28.188685504608678	27.600297653413392	21.820799448681363
39	24.42132620724669	25.15185089470144	27.931354597957114	22.49546830009476
40	25.0694646820662	24.591642977193956	28.671272596641135	21.667619744098708
41	20.919912170248956	25.7334572333995	28.155694362730273	25.190936233621276
42	22.010315230360646	25.151667610579892	29.027118718624052	23.81089844043541
43	22.625370921240982	23.352275747295185	29.7256603268689	24.296693004594932
44	22.548849800495233	24.70349211236783	27.911055881495816	24.836602205641118
45	23.55324678657114	23.60131304744676	27.17984387858527	25.665596287396834
46	22.871796422660516	25.705139836620532	27.705411097120425	23.717652643598527
47	20.668858744760364	25.73680216861773	30.205956367382026	23.388382719239882
48	21.25440569207168	24.13883955491284	30.421956704624808	24.184798048390675
49	22.785836169655113	23.455831275969068	30.420627894743596	23.337704659632223
50	20.90094226366887	23.571575198725807	29.403767588402513	26.123714949202803
51	20.485208054970574	23.258480098093663	27.904870042393615	28.351441804542148
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	854.0
1	967.5
2	1081.0
3	3020.5
4	4960.0
5	4028.5
6	3097.0
7	3038.5
8	2980.0
9	3206.5
10	3433.0
11	3760.5
12	4088.0
13	4019.0
14	3950.0
15	4259.5
16	4569.0
17	4787.0
18	5005.0
19	5132.0
20	5259.0
21	5738.0
22	6217.0
23	7190.5
24	8164.0
25	10454.5
26	15112.0
27	17479.0
28	21188.5
29	24898.0
30	27988.0
31	31078.0
32	34213.0
33	37348.0
34	42657.0
35	47966.0
36	52290.0
37	56614.0
38	63899.5
39	71185.0
40	81571.5
41	91958.0
42	102681.5
43	113405.0
44	134919.5
45	156434.0
46	184669.5
47	212905.0
48	216022.5
49	219140.0
50	195827.0
51	172514.0
52	160173.0
53	147832.0
54	137756.0
55	127680.0
56	122756.0
57	117832.0
58	113607.5
59	109383.0
60	103001.5
61	96620.0
62	90542.0
63	84464.0
64	72816.0
65	61168.0
66	51536.0
67	41904.0
68	34020.5
69	26137.0
70	21681.5
71	17226.0
72	14829.0
73	12432.0
74	10884.5
75	7327.5
76	5318.0
77	4188.0
78	3058.0
79	2341.5
80	1625.0
81	1207.0
82	789.0
83	479.0
84	169.0
85	116.5
86	64.0
87	44.0
88	24.0
89	13.0
90	2.0
91	8.0
92	14.0
93	7.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2182404.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.144922238899895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.85913296150747	19.23465654155989
2	11.343949459302971	6.158612519095793
3	4.848179863320329	3.9481039617009195
4	2.4975105792023644	2.7117892185311203
5	1.471336205371284	1.9969653441040773
6	0.922753291620247	1.5028839808032313
7	0.6705613929308541	1.2741635807261504
8	0.5081789554306496	1.1035582582888288
9	0.3928130458164151	0.9596591624800808
>10	5.4268185527073225	37.48252063340662
>50	0.9640526819938698	16.756660653790657
>100	0.08754572373104297	4.089070696159524
>500	0.0046075416847744955	0.77896296381466
>1k	0.0020477963043442206	0.9789844955553654
>5k	5.119490760860551E-4	1.0234079899831876
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	8241	0.37761111141658465	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	7705	0.3530510391293271	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT	6147	0.28166187378688823	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC	3174	0.1454359504473049	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2435	0.11157420899155243	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2366	0.10841255789487189	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5821030386674514E-5	0.0	0.0	0.29041369059074307	0.0
2	4.5821030386674514E-5	0.0	0.0	1.2933444036942747	0.0
3	4.5821030386674514E-5	0.0	0.0	1.7082538338456124	0.0
4	4.5821030386674514E-5	0.0	0.0	2.1398879400880864	0.0
5	4.5821030386674514E-5	0.0	0.0	3.2365226603323674	0.0
6	4.5821030386674514E-5	0.0	0.0	3.8871812918231456	0.0
7	4.5821030386674514E-5	0.0	0.0	4.621921514073471	0.0
8	4.5821030386674514E-5	0.0	0.0	5.93808479090031	0.0
9	4.5821030386674514E-5	0.0	0.0	6.433822518653741	0.0
10	4.5821030386674514E-5	0.0	0.0	7.7782573712291585	0.0
11	4.5821030386674514E-5	0.0	0.0	9.710209475422516	0.0
12	4.5821030386674514E-5	0.0	0.0	11.136847256511627	0.0
13	4.5821030386674514E-5	0.0	0.0	11.685920663635148	0.0
14	4.5821030386674514E-5	0.0	0.0	11.894085604681809	0.0
15	4.5821030386674514E-5	0.0	0.0	12.286588550974063	0.0
16	4.5821030386674514E-5	0.0	0.0	13.158516938202093	0.0
17	4.5821030386674514E-5	0.0	0.0	14.220190212261342	0.0
18	4.5821030386674514E-5	0.0	0.0	15.398844576897769	0.0
19	4.5821030386674514E-5	0.0	0.0	16.098440068841516	0.0
20	9.164206077334903E-5	0.0	0.0	16.79679839296482	0.0
21	3.665682430933961E-4	0.0	0.0	17.653651661195635	0.0
22	3.665682430933961E-4	0.0	0.0	18.580702747978833	0.0
23	4.582103038667451E-4	0.0	0.0	19.460833099646077	0.0
24	5.498523646400941E-4	0.0	0.0	20.171883849186493	0.0
25	5.956733950267687E-4	0.0	0.0	20.814340516238058	0.0
26	5.956733950267687E-4	0.0	0.0	21.413496309574214	0.0
27	5.956733950267687E-4	0.0	0.0	21.988870988139684	0.0
28	5.956733950267687E-4	0.0	0.0	22.557830722451023	0.0
29	5.956733950267687E-4	0.0	0.0	23.1778809056435	0.0
30	5.956733950267687E-4	0.0	0.0	23.900432733810973	0.0
31	6.873154558001177E-4	0.0	0.0	24.519612317426105	0.0
32	6.873154558001177E-4	0.0	0.0	25.145573413538465	0.0
33	6.873154558001177E-4	0.0	0.0	25.736573063465794	0.0
34	6.873154558001177E-4	0.0	0.0	26.35511115265551	0.0
35	7.331364861867922E-4	0.0	0.0	27.002379027897675	0.0
36	7.331364861867922E-4	0.0	0.0	27.627561166493464	0.0
37	7.331364861867922E-4	0.0	0.0	28.261220195710784	0.0
38	7.331364861867922E-4	0.0	0.0	28.895749824505454	0.0
39	7.331364861867922E-4	0.0	0.0	29.671087479678373	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	85	0.0	45.000004	1
GCGTAAG	305	0.0	40.573772	1
CGAATAT	150	0.0	40.5	14
CGTAAGG	440	0.0	38.86364	2
GCCGATC	105	0.0	38.57143	9
ACGTAAG	65	9.094947E-12	38.076927	1
GGACGTA	30	1.14025825E-4	37.499996	8
CAATGCG	30	1.14025825E-4	37.499996	1
TAGGGCG	730	0.0	36.369865	5
GACGTAA	25	0.0021075227	36.0	18
CGTAGTA	25	0.0021075227	36.0	39
GCGATCG	25	0.0021075227	36.0	9
ACGTAGG	390	0.0	35.769234	2
TACGAAT	170	0.0	35.735294	12
AACGGGA	680	0.0	35.404415	4
TCGTTAC	70	2.1827873E-11	35.357143	24
TCGGTTA	70	2.1827873E-11	35.357143	13
TATGCGG	300	0.0	35.25	2
GGCCGAT	320	0.0	35.156254	8
CACAACG	480	0.0	35.15625	12
>>END_MODULE