##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552732_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	653800
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.989547262159682	33.0	33.0	33.0	27.0	33.0
2	32.12273631079841	33.0	33.0	33.0	33.0	33.0
3	32.14383909452432	33.0	33.0	33.0	33.0	33.0
4	32.103374120526155	33.0	33.0	33.0	33.0	33.0
5	32.13394004282655	33.0	33.0	33.0	33.0	33.0
6	35.640388498011625	37.0	37.0	37.0	33.0	37.0
7	35.99890180483328	37.0	37.0	37.0	37.0	37.0
8	36.06165035178954	37.0	37.0	37.0	37.0	37.0
9	35.946726827776075	37.0	37.0	37.0	37.0	37.0
10	35.945888650963596	37.0	37.0	37.0	33.0	37.0
11	35.90952584888345	37.0	37.0	37.0	33.0	37.0
12	35.890032119914345	37.0	37.0	37.0	33.0	37.0
13	35.92249311716121	37.0	37.0	37.0	37.0	37.0
14	35.94061027837259	37.0	37.0	37.0	37.0	37.0
15	35.96092230039768	37.0	37.0	37.0	37.0	37.0
16	35.978291526460694	37.0	37.0	37.0	37.0	37.0
17	35.98305750994188	37.0	37.0	37.0	37.0	37.0
18	35.97336035484858	37.0	37.0	37.0	37.0	37.0
19	35.97005659223004	37.0	37.0	37.0	37.0	37.0
20	35.959687977974916	37.0	37.0	37.0	37.0	37.0
21	35.86607372285103	37.0	37.0	37.0	37.0	37.0
22	35.914014989293364	37.0	37.0	37.0	37.0	37.0
23	35.93119914346895	37.0	37.0	37.0	37.0	37.0
24	35.95256806362802	37.0	37.0	37.0	37.0	37.0
25	35.945299785867235	37.0	37.0	37.0	37.0	37.0
26	35.904420312022026	37.0	37.0	37.0	37.0	37.0
27	35.89413276231264	37.0	37.0	37.0	37.0	37.0
28	35.877727133680025	37.0	37.0	37.0	37.0	37.0
29	35.891032425818295	37.0	37.0	37.0	37.0	37.0
30	35.85997705720404	37.0	37.0	37.0	37.0	37.0
31	35.81680483328235	37.0	37.0	37.0	33.0	37.0
32	35.79830223309881	37.0	37.0	37.0	33.0	37.0
33	35.75299785867238	37.0	37.0	37.0	33.0	37.0
34	35.69670847353931	37.0	37.0	37.0	33.0	37.0
35	35.682955032119914	37.0	37.0	37.0	33.0	37.0
36	35.671852248394	37.0	37.0	37.0	33.0	37.0
37	35.665619455490976	37.0	37.0	37.0	33.0	37.0
38	35.65685377791373	37.0	37.0	37.0	33.0	37.0
39	35.64512695013766	37.0	37.0	37.0	33.0	37.0
40	35.62253747323341	37.0	37.0	37.0	33.0	37.0
41	35.60919853166106	37.0	37.0	37.0	33.0	37.0
42	35.614587029672684	37.0	37.0	37.0	33.0	37.0
43	35.61620220250841	37.0	37.0	37.0	33.0	37.0
44	35.57796115019884	37.0	37.0	37.0	33.0	37.0
45	35.53726674824105	37.0	37.0	37.0	33.0	37.0
46	35.54962526766595	37.0	37.0	37.0	33.0	37.0
47	35.53883450596513	37.0	37.0	37.0	33.0	37.0
48	35.5450994187825	37.0	37.0	37.0	33.0	37.0
49	35.53637656775772	37.0	37.0	37.0	33.0	37.0
50	35.518826858366474	37.0	37.0	37.0	33.0	37.0
51	35.1980483328235	37.0	37.0	37.0	33.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	392.0
15	1500.0
16	1121.0
17	661.0
18	567.0
19	518.0
20	534.0
21	610.0
22	697.0
23	924.0
24	1387.0
25	2035.0
26	2975.0
27	4496.0
28	6508.0
29	7956.0
30	9348.0
31	11675.0
32	14984.0
33	21120.0
34	32525.0
35	68822.0
36	462445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.266289385133064	19.626797185683696	25.581982257571124	12.524931171612113
2	30.28433771795656	23.880238605078006	26.1408687672071	19.694554909758335
3	30.7089323952279	24.495105536861423	27.432395227898436	17.363566840012236
4	28.244723156928725	26.525390027531355	26.778219639033345	18.45166717650658
5	24.143927806668707	30.700520036708472	26.301315386968493	18.854236769654328
6	25.148516365861116	35.46497399816458	24.512083205873356	14.874426430100948
7	80.16717650657694	6.453808504129703	8.32058733557663	5.058427653716733
8	81.27944325481799	4.7162740899357605	7.90868767207097	6.095594983175283
9	76.12740899357601	6.955490975833587	10.904099112878555	6.013000917711839
10	39.12236157846436	25.163352707249924	19.685377791373508	16.028907922912204
11	28.64698684613031	26.971856836953194	23.937595594983176	20.443560721933313
12	24.542061792597124	23.62878556133374	31.404557968797796	20.424594677271337
13	24.167023554603855	25.676506576934845	32.65478739675742	17.501682471703887
14	21.281431630468035	28.04343836035485	30.218109513612724	20.457020495564393
15	19.50107066381156	26.32119914346895	34.09605383909452	20.08167635362496
16	22.0414499847048	26.092994799632912	30.158458244111348	21.707096971550932
17	21.94249005812175	26.13520954420312	28.119914346895076	23.802386050780054
18	22.917100030590394	24.447996329152648	32.285102477821965	20.349801162434996
19	21.587029672682778	27.336647292750076	29.38895686754359	21.687366167023555
20	22.92673600489446	27.315845824411134	30.07464056286326	19.68277760783114
21	22.29060874885286	28.382991740593454	29.86417864790456	19.46222086264913
22	20.88635668400122	26.291373508718262	27.592382991740593	25.22988681553992
23	19.11777301927195	28.39568675435913	28.33435301315387	24.15218721321505
24	20.958397063322117	25.948914040991127	29.195472621596817	23.897216274089935
25	19.17834200061181	28.08504129703273	28.15769348424595	24.578923218109512
26	19.174059345365553	27.47017436524931	31.026001835423678	22.329764453961456
27	22.805445090241662	26.69302539002753	28.88467421229734	21.616855307433465
28	20.163505659223006	25.99984704802692	30.362496176200672	23.4741511165494
29	21.257877026613645	24.75099418782502	31.066228204343837	22.924900581217496
30	25.688436830835116	25.276690119302543	27.72468644845518	21.310186601407157
31	24.736463750382377	26.562710308962984	26.014377485469563	22.686448455185072
32	23.314622208626492	25.225298256347507	28.5672988681554	22.892780666870603
33	25.16243499541144	24.13475068828388	27.649434077699603	23.05338023860508
34	22.955185071887428	23.318598959926582	30.495258488834505	23.23095747935148
35	22.350871826246557	22.994952584888345	30.50061180789232	24.153563780972775
36	27.61410217191802	23.468491893545426	28.881921076781893	20.035484857754664
37	25.688589782808197	24.322881615172836	29.40838176812481	20.580146833894158
38	23.445396145610278	25.511471397981033	28.462526766595285	22.5806056898134
39	25.28143163046803	22.4585500152952	30.3380238605078	21.92199449372897
40	26.645763230345672	21.325940654634444	28.980575099418783	23.047721015601102
41	22.217344753747323	22.238146222086264	28.06531049250535	27.47919853166106
42	23.027072499235242	21.60828999694096	31.239063933924747	24.12557356989905
43	23.9788926277149	21.35071887427348	32.103395533802384	22.566992964209238
44	23.610125420617926	22.548791679412663	29.677271336800242	24.163811563169165
45	24.8372591006424	21.27745487916794	29.064086876720708	24.82119914346895
46	25.859437136739068	22.52278984398899	29.24013459773631	22.377638421535636
47	21.708473539308656	22.717344753747323	31.983175282961152	23.59100642398287
48	22.50642398286938	21.703273172223923	31.069287243805444	24.721015601101254
49	24.71918017742429	19.56087488528602	32.799938819210766	22.920006118078923
50	23.387427347812785	20.001835423676965	30.418170694401958	26.19256653410829
51	22.457173447537475	19.54359131232793	27.971397981033956	30.027837259100643
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	589.0
1	505.5
2	422.0
3	983.0
4	1544.0
5	1269.0
6	994.0
7	1013.0
8	1032.0
9	1163.0
10	1294.0
11	1366.0
12	1438.0
13	1433.0
14	1428.0
15	1468.5
16	1509.0
17	1431.0
18	1353.0
19	1406.5
20	1460.0
21	1682.0
22	1904.0
23	2035.0
24	2166.0
25	2461.5
26	3226.0
27	3695.0
28	4791.5
29	5888.0
30	7394.0
31	8900.0
32	9932.0
33	10964.0
34	12811.5
35	14659.0
36	16406.0
37	18153.0
38	19823.0
39	21493.0
40	24277.5
41	27062.0
42	29934.0
43	32806.0
44	36344.0
45	39882.0
46	44496.0
47	49110.0
48	56777.5
49	64445.0
50	75279.5
51	86114.0
52	85812.5
53	85511.0
54	70812.0
55	56113.0
56	46376.5
57	36640.0
58	29778.0
59	22916.0
60	19543.5
61	16171.0
62	14024.0
63	11877.0
64	9973.0
65	8069.0
66	6467.5
67	4866.0
68	3990.5
69	3115.0
70	2555.5
71	1996.0
72	1645.5
73	1295.0
74	1103.0
75	781.0
76	651.0
77	514.0
78	377.0
79	247.5
80	118.0
81	97.5
82	77.0
83	54.5
84	32.0
85	17.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	653800.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.398956977767995
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.60804974697493	26.032684577934496
2	12.431161015704575	9.298249120200841
3	4.77372489127548	5.355969954975356
4	2.410792876441773	3.6064455627342165
5	1.59178961532391	2.9765635670578385
6	1.1282771755812617	2.531783372913672
7	0.8721007915052761	2.2830961987248264
8	0.7405550155877763	2.215678813411004
9	0.6428744365101018	2.1638550053833328
>10	5.690303700881066	36.528663616005325
>50	0.07124678002573231	1.8286858141034046
>100	0.03459381212425011	2.282276282923201
>500	0.0028828176770208424	0.8099939918945279
>1k	0.001647324386869053	2.086054121737875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	4952	0.757418170694402	No Hit
GCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	3787	0.5792291220556746	No Hit
CTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGCT	3204	0.4900581217497706	No Hit
TCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	1601	0.24487610890180483	No Hit
ACTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTGC	968	0.14805750994187825	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCC	885	0.13536249617620066	No Hit
ACCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	754	0.11532578770266136	No Hit
CGCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	731	0.11180789232181096	No Hit
GCCTGTCTCTTATACACATCTGACGCTGGTGGCTTCGTATGCCGTCTTCTG	717	0.10966656469868462	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	696	0.1064545732639951	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5044356072193331	0.0
2	0.0	0.0	0.0	2.2794432548179873	0.0
3	0.0	0.0	0.0	2.982869379014989	0.0
4	0.0	0.0	0.0	3.680789232181095	0.0
5	0.0	0.0	0.0	5.410981951667177	0.0
6	0.0	0.0	0.0	6.3716732945855	0.0
7	0.0	0.0	0.0	7.546344447843377	0.0
8	0.0	0.0	0.0	9.595900887121443	0.0
9	0.0	0.0	0.0	10.357142857142858	0.0
10	0.0	0.0	0.0	12.410981951667177	0.0
11	0.0	0.0	0.0	15.258794738452126	0.0
12	0.0	0.0	0.0	17.373814622208627	0.0
13	0.0	0.0	0.0	18.201437748546955	0.0
14	0.0	0.0	0.0	18.497399816457634	0.0
15	0.0	0.0	0.0	19.06072193331294	0.0
16	0.0	0.0	0.0	20.365096359743042	0.0
17	0.0	0.0	0.0	21.931171612113797	0.0
18	0.0	0.0	0.0	23.751300091771185	0.0
19	0.0	0.0	0.0	24.765218721321506	0.0
20	0.0	0.0	0.0	25.74701743652493	0.0
21	0.0	0.0	0.0	26.90914652799021	0.0
22	0.0	0.0	0.0	28.1009483022331	0.0
23	1.5295197308045274E-4	0.0	0.0	29.353166105842764	0.0
24	1.5295197308045274E-4	0.0	0.0	30.25971245029061	0.0
25	9.177118384827164E-4	0.0	0.0	31.133680024472316	0.0
26	9.177118384827164E-4	0.0	0.0	31.946466809421842	0.0
27	9.177118384827164E-4	0.0	0.0	32.72162740899358	0.0
28	9.177118384827164E-4	0.0	0.0	33.483328234934234	0.0
29	9.177118384827164E-4	0.0	0.0	34.297797491587644	0.0
30	9.177118384827164E-4	0.0	0.0	35.238146222086264	0.0
31	9.177118384827164E-4	0.0	0.0	36.027225451208324	0.0
32	9.177118384827164E-4	0.0	0.0	36.802539002753136	0.0
33	9.177118384827164E-4	0.0	0.0	37.587029672682775	0.0
34	9.177118384827164E-4	0.0	0.0	38.28785561333741	0.0
35	9.177118384827164E-4	0.0	0.0	39.04282655246253	0.0
36	9.177118384827164E-4	0.0	0.0	39.72239216885898	0.0
37	9.177118384827164E-4	0.0	0.0	40.44279596206791	0.0
38	9.177118384827164E-4	0.0	0.0	41.173600489446315	0.0
39	9.177118384827164E-4	0.0	0.0	41.95870296726828	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCA	35	1.2108467E-7	45.000004	31
TCGCTAG	35	1.2108467E-7	45.000004	1
TCGTTCG	20	7.031052E-4	45.0	45
TAGCGTG	20	7.031052E-4	45.0	1
ACTCGCG	20	7.031052E-4	45.0	10
ACGTCAA	20	7.031052E-4	45.0	32
CACGTAG	20	7.031052E-4	45.0	1
CGTCAAG	20	7.031052E-4	45.0	33
GCGTACG	25	3.8889535E-5	45.0	1
TGCGTAG	85	0.0	42.35294	1
TATACGG	60	3.6379788E-12	41.250004	2
TGTTGCG	50	1.0804797E-9	40.5	1
TGTAGCG	50	1.0804797E-9	40.5	1
TCGAGAG	50	1.0804797E-9	40.5	1
CGGACGG	90	0.0	40.0	2
CGTTTTT	820	0.0	39.786587	1
ACACGTA	40	3.4551704E-7	39.375	1
GCGCGAG	80	0.0	39.375	1
CGCATGG	80	0.0	39.375	2
TAAACGG	40	3.4551704E-7	39.375	2
>>END_MODULE