FastQCFastQC Report
Sat 18 Jun 2016
SRR3552730_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552730_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570119
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC87780.5590659051957209No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC69790.44448860245624694No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT63090.40181667758940565No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC32860.20928350016782168No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC21870.13928880549818198No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19230.12247479331184451No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC16640.10597922832600586No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG16300.10381378736261393No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA253.8914604E-545.08
CGTAAGG2300.039.1304362
TGCGTAG1350.038.3333361
CCATCGT301.1401125E-437.49999623
CCGCATA301.1401125E-437.4999961
TTACACG6350.036.1417334
CGTTTTT19000.036.1184231
TAATACG250.00210734336.01
CGAATGG1250.036.02
CGAATAC250.00210734336.014
ACTAACG250.00210734336.01
CGACAAT458.7026274E-735.00000420
TCATCGA458.7026274E-735.00000416
CACAACG3150.035.012
CGCACGG1500.034.52
AACGAGC3400.034.41176215
TCGAAAG850.034.4117621
AATACGG1250.034.1999972
CTATCGG401.5609297E-533.752
CGACAGG2000.033.752