##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552730_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1570119
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22772668823191	33.0	33.0	33.0	27.0	33.0
2	31.443642806691724	33.0	33.0	33.0	27.0	33.0
3	31.53984952732882	33.0	33.0	33.0	27.0	33.0
4	31.45725196625224	33.0	33.0	33.0	27.0	33.0
5	31.56402476500189	33.0	33.0	33.0	27.0	33.0
6	34.93439223396443	37.0	37.0	37.0	27.0	37.0
7	35.28814694937135	37.0	37.0	37.0	33.0	37.0
8	35.39518151171981	37.0	37.0	37.0	33.0	37.0
9	35.254819539155946	37.0	37.0	37.0	33.0	37.0
10	35.31879812931376	37.0	37.0	37.0	33.0	37.0
11	35.31595248513011	37.0	37.0	37.0	33.0	37.0
12	35.325742188967844	37.0	37.0	37.0	33.0	37.0
13	35.38986726483789	37.0	37.0	37.0	33.0	37.0
14	35.446288466033465	37.0	37.0	37.0	33.0	37.0
15	35.47794148086865	37.0	37.0	37.0	33.0	37.0
16	35.490069860946846	37.0	37.0	37.0	33.0	37.0
17	35.490665357211775	37.0	37.0	37.0	33.0	37.0
18	35.49071121360865	37.0	37.0	37.0	33.0	37.0
19	35.47447295396081	37.0	37.0	37.0	33.0	37.0
20	35.45009008871302	37.0	37.0	37.0	33.0	37.0
21	35.37325068991586	37.0	37.0	37.0	33.0	37.0
22	35.39829274086868	37.0	37.0	37.0	33.0	37.0
23	35.42107063222596	37.0	37.0	37.0	33.0	37.0
24	35.421972474697775	37.0	37.0	37.0	33.0	37.0
25	35.40831363737398	37.0	37.0	37.0	33.0	37.0
26	35.35843652614866	37.0	37.0	37.0	33.0	37.0
27	35.346965421092285	37.0	37.0	37.0	33.0	37.0
28	35.32861458271634	37.0	37.0	37.0	33.0	37.0
29	35.31275018008189	37.0	37.0	37.0	33.0	37.0
30	35.28852526464554	37.0	37.0	37.0	33.0	37.0
31	35.26323164040433	37.0	37.0	37.0	33.0	37.0
32	35.233077238094694	37.0	37.0	37.0	33.0	37.0
33	35.213796533893294	37.0	37.0	37.0	33.0	37.0
34	35.182822448489574	37.0	37.0	37.0	33.0	37.0
35	35.144381413128556	37.0	37.0	37.0	33.0	37.0
36	35.13024554189842	37.0	37.0	37.0	33.0	37.0
37	35.12811258254948	37.0	37.0	37.0	33.0	37.0
38	35.07576623173148	37.0	37.0	37.0	33.0	37.0
39	35.09247643013045	37.0	37.0	37.0	33.0	37.0
40	35.076450256318154	37.0	37.0	37.0	33.0	37.0
41	35.059094883890964	37.0	37.0	37.0	33.0	37.0
42	35.03257205345582	37.0	37.0	37.0	33.0	37.0
43	35.031577861295865	37.0	37.0	37.0	27.0	37.0
44	34.98690099285468	37.0	37.0	37.0	27.0	37.0
45	34.94140762579142	37.0	37.0	37.0	27.0	37.0
46	34.959461034482096	37.0	37.0	37.0	27.0	37.0
47	34.91762853643577	37.0	37.0	37.0	27.0	37.0
48	34.907117231241706	37.0	37.0	37.0	27.0	37.0
49	34.87904738430654	37.0	37.0	37.0	27.0	37.0
50	34.86189263361567	37.0	37.0	37.0	27.0	37.0
51	34.412975067494884	37.0	37.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	773.0
15	3623.0
16	4316.0
17	4149.0
18	3946.0
19	3982.0
20	4223.0
21	4588.0
22	5319.0
23	6317.0
24	7783.0
25	9918.0
26	13069.0
27	16825.0
28	21732.0
29	25602.0
30	29841.0
31	36671.0
32	46126.0
33	61641.0
34	92082.0
35	187477.0
36	980116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56727101576377	19.253317742158398	24.583996499628373	12.595414742449456
2	29.37038530200577	24.225042815226107	26.15534236576973	20.249229516998394
3	31.694604039566425	24.31599133568857	26.082354267415397	17.907050357329606
4	28.81883475074182	26.792873661168358	25.63461750351406	18.753674084575756
5	25.35043522178892	29.9547359149211	25.099753585556257	19.595075277733727
6	26.36220566721376	35.40489606201823	23.380902976143847	14.851995294624166
7	79.80089407236012	6.97240145492157	8.067477687996897	5.159226784721413
8	81.25275854887433	5.479457289543022	7.500705360549105	5.767078801033552
9	75.24894609898995	8.042065601397091	10.298837221892098	6.41015107772086
10	40.63825735501577	27.29786723171938	16.971643550584385	15.092231862680471
11	29.95734718196519	26.11763821723067	23.905194447045098	20.019820153759046
12	27.096990737644727	23.049654198184978	30.070778074782865	19.78257698938743
13	24.822322384481684	24.477953581862266	30.86008130593923	19.839642727716818
14	21.021846114848618	27.278951468009748	29.654503894290812	22.044698522850815
15	20.561371462927333	25.632006236469973	33.30148861328345	20.50513368731924
16	23.08188105487546	25.798681501211057	29.776914998162557	21.342522445750927
17	22.797125568189415	25.48138071063403	28.08869900943814	23.63279471173841
18	23.431981907103857	24.933460457455773	30.65060673745111	20.983950897989263
19	23.65680563065602	27.101130551251213	28.154108064420598	21.087955753672176
20	24.88263628425616	26.974643323213083	28.234229380066093	19.908491012464662
21	24.172053201063104	27.570012209265666	28.354666111294748	19.90326847837648
22	21.802423892711317	26.79153618292626	27.290479256667805	24.115560667694613
23	21.537794269096803	27.083615955223777	27.66083335084793	23.717756424831492
24	22.69948965651648	25.44278490993358	27.950938750502353	23.90678668304759
25	21.54887623167416	27.091449756356045	26.32673064907819	25.0329433628916
26	21.223423192764372	27.396585863874012	27.890497471847674	23.489493471513942
27	22.464730380308755	26.067896764512753	27.634593301526827	23.832779553651665
28	20.5697147795804	26.63288578763775	28.33313908054103	24.464260352240817
29	22.980423776796535	24.871490632238704	27.851137397866022	24.29694819309874
30	23.46586468923693	24.67545453561163	27.162081345426685	24.696599429724753
31	23.822079727714907	27.059732415186367	25.80823491722602	23.309952939872712
32	24.41216239023921	27.150298799008226	25.660793863395064	22.776744947357493
33	23.005007900675047	26.845226380930363	25.105039809084534	25.044725909310056
34	22.743944885706117	24.691440585076673	28.872779706506325	23.691834822710888
35	21.947826884459076	25.872752320047077	27.14488519659975	25.0345355988941
36	23.05201070746867	27.80553575875459	25.57423991429949	23.56821361947725
37	22.781075829284276	26.957065037745547	28.439882582148236	21.821976550821944
38	20.980511668223873	28.791129844298425	27.10730842694089	23.121050060536813
39	21.654982838880365	25.37826750711252	28.754890552881662	24.21185910112546
40	22.555933658531615	23.99525131534616	29.820287506870496	23.628527519251723
41	20.10287118364914	27.51746842118336	26.726127127943805	25.653533267223693
42	22.087688894918156	27.04489277564312	26.731540730352286	24.135877599086438
43	22.474857001284615	24.594887393885433	28.60445609536602	24.325799509463934
44	22.246211911326466	23.395933684007392	27.996731457934082	26.361122946732063
45	22.477659336649005	22.634844874815222	27.87801434158812	27.009481446947653
46	22.335440816906235	24.530115233303974	28.487267525582453	24.647176424207338
47	19.87352550984989	25.49329063593269	30.66410889875226	23.969074955465157
48	20.488701811773502	24.974794904080518	29.405669251821042	25.130834032324938
49	22.790756624179444	23.314793337320292	29.97779149223721	23.916658546263054
50	21.088847405833572	22.909218982765	29.011176859843108	26.99075675155832
51	19.7103531643143	22.77992941936248	27.65981431980633	29.84990309651689
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	912.0
1	897.0
2	882.0
3	2376.5
4	3871.0
5	3084.0
6	2297.0
7	2305.5
8	2314.0
9	2616.5
10	2919.0
11	2972.5
12	3026.0
13	3135.0
14	3244.0
15	3269.5
16	3295.0
17	3293.0
18	3291.0
19	3560.5
20	3830.0
21	4127.5
22	4425.0
23	5200.0
24	5975.0
25	7771.0
26	10447.0
27	11327.0
28	14284.0
29	17241.0
30	19731.0
31	22221.0
32	25572.0
33	28923.0
34	33991.5
35	39060.0
36	40537.0
37	42014.0
38	46161.0
39	50308.0
40	57607.0
41	64906.0
42	71579.5
43	78253.0
44	87680.5
45	97108.0
46	114323.5
47	131539.0
48	154345.5
49	177152.0
50	179818.0
51	182484.0
52	157620.0
53	132756.0
54	115807.5
55	98859.0
56	89776.0
57	80693.0
58	74050.5
59	67408.0
60	62008.5
61	56609.0
62	51792.5
63	46976.0
64	40146.5
65	33317.0
66	27518.5
67	21720.0
68	17759.0
69	13798.0
70	11575.0
71	9352.0
72	8043.0
73	6734.0
74	5417.0
75	3298.5
76	2497.0
77	2030.0
78	1563.0
79	1198.0
80	833.0
81	568.0
82	303.0
83	225.0
84	147.0
85	95.5
86	44.0
87	33.0
88	22.0
89	12.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1570119.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.153158292289405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.65913387066664	21.004428901059896
2	12.175047875601473	7.342322916184261
3	5.023202914389213	4.543962978356022
4	2.5686578724218676	3.098125897034876
5	1.4930201769848643	2.2509636865103277
6	1.00460933532018	1.817528658589262
7	0.7134582459434291	1.5059113597409954
8	0.5297994641896511	1.278010168550453
9	0.4230065131407046	1.1479484114460954
>10	5.947552778686131	41.949757725732375
>50	0.3916832979380951	7.3387881682315985
>100	0.06465948362620032	3.581973028101191
>500	0.003828519987851158	0.7737177314758035
>1k	0.0017015644390449588	0.9513700952181754
>5k	6.380866646418596E-4	1.4151902737686568
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	8778	0.5590659051957209	No Hit
GCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	6979	0.44448860245624694	No Hit
CTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGCT	6309	0.40181667758940565	No Hit
TCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	3286	0.20928350016782168	No Hit
ACTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTGC	2187	0.13928880549818198	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1923	0.12247479331184451	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCC	1664	0.10597922832600586	No Hit
GCCTGTCTCTTATACACATCTGACGCGCGTCTAATCGTATGCCGTCTTCTG	1630	0.10381378736261393	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.273788801995263E-4	0.0	0.0	0.41168854080486894	0.0
2	1.273788801995263E-4	0.0	0.0	1.8072515522708787	0.0
3	1.273788801995263E-4	0.0	0.0	2.392302749027303	0.0
4	1.273788801995263E-4	0.0	0.0	2.986525225158093	0.0
5	1.273788801995263E-4	0.0	0.0	4.41800908084037	0.0
6	1.273788801995263E-4	0.0	0.0	5.198968995343665	0.0
7	1.273788801995263E-4	0.0	0.0	6.122911702870929	0.0
8	1.273788801995263E-4	0.0	0.0	7.77367830081669	0.0
9	1.273788801995263E-4	0.0	0.0	8.416495819743599	0.0
10	1.9106832029928942E-4	0.0	0.0	10.076370007623625	0.0
11	1.9106832029928942E-4	0.0	0.0	12.4493748563007	0.0
12	1.9106832029928942E-4	0.0	0.0	14.199624359682291	0.0
13	1.9106832029928942E-4	0.0	0.0	14.854288114467757	0.0
14	1.9106832029928942E-4	0.0	0.0	15.10579771342172	0.0
15	1.9106832029928942E-4	0.0	0.0	15.580793557685755	0.0
16	1.9106832029928942E-4	0.0	0.0	16.60874112089593	0.0
17	1.9106832029928942E-4	0.0	0.0	17.86501532686376	0.0
18	2.547577603990526E-4	0.0	0.0	19.29567121982474	0.0
19	2.547577603990526E-4	0.0	0.0	20.154268561809648	0.0
20	2.547577603990526E-4	0.0	0.0	20.950705010257185	0.0
21	4.4582608069834197E-4	0.0	0.0	21.918338673692887	0.0
22	4.4582608069834197E-4	0.0	0.0	22.979086298554442	0.0
23	4.4582608069834197E-4	0.0	0.0	23.988627613575787	0.0
24	5.732049608978682E-4	0.0	0.0	24.82913715457236	0.0
25	6.368944009976314E-4	0.0	0.0	25.57385777765889	0.0
26	6.368944009976314E-4	0.0	0.0	26.263168587858626	0.0
27	0.001337478242095026	0.0	0.0	26.93184401946604	0.0
28	0.001337478242095026	0.0	0.0	27.589246420175794	0.0
29	0.001337478242095026	0.0	0.0	28.274353727328947	0.0
30	0.001337478242095026	0.0	0.0	29.087413119642523	0.0
31	0.001337478242095026	0.0	0.0	29.79869678667668	0.0
32	0.001337478242095026	0.0	0.0	30.464060367398904	0.0
33	0.001337478242095026	0.0	0.0	31.123373451311654	0.0
34	0.001337478242095026	0.0	0.0	31.768101653441555	0.0
35	0.0014648571222945523	0.0	0.0	32.44989710970952	0.0
36	0.0015285465623943153	0.0	0.0	33.13710616838596	0.0
37	0.0015922360024940784	0.0	0.0	33.81367909056575	0.0
38	0.0015922360024940784	0.0	0.0	34.493946000271315	0.0
39	0.0015922360024940784	0.0	0.0	35.21229919515655	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	25	3.8914604E-5	45.0	8
CGTAAGG	230	0.0	39.130436	2
TGCGTAG	135	0.0	38.333336	1
CCATCGT	30	1.1401125E-4	37.499996	23
CCGCATA	30	1.1401125E-4	37.499996	1
TTACACG	635	0.0	36.14173	34
CGTTTTT	1900	0.0	36.118423	1
TAATACG	25	0.002107343	36.0	1
CGAATGG	125	0.0	36.0	2
CGAATAC	25	0.002107343	36.0	14
ACTAACG	25	0.002107343	36.0	1
CGACAAT	45	8.7026274E-7	35.000004	20
TCATCGA	45	8.7026274E-7	35.000004	16
CACAACG	315	0.0	35.0	12
CGCACGG	150	0.0	34.5	2
AACGAGC	340	0.0	34.411762	15
TCGAAAG	85	0.0	34.411762	1
AATACGG	125	0.0	34.199997	2
CTATCGG	40	1.5609297E-5	33.75	2
CGACAGG	200	0.0	33.75	2
>>END_MODULE