SRR3552728_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329129
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	3984	1.2104676281944162	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	2948	0.8956974317061093	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2602	0.7905714780526785	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1732	0.5262374327391388	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1178	0.3579143739992526	No Hit
ACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1146	0.3481917424474902	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	866	0.2631187163695694	No Hit
GCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	747	0.22696268028645303	TruSeq Adapter, Index 19 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	640	0.19445263103524757	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	614	0.18655299289944063	No Hit
CGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	596	0.1810840126515743	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	555	0.16862689097587874	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG	508	0.15434677588422777	No Hit
GGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	492	0.14948546010834657	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC	465	0.14128198973654707	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	382	0.1160639141491634	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	367	0.11150643060927477	No Hit
TGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	364	0.11059493390129707	TruSeq Adapter, Index 13 (95% over 21bp)
AGGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	346	0.10512595365343072	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG	337	0.10239146352949756	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGG	35	1.208391E-7	45.000004	2
AGTGCAG	30	2.160763E-6	45.000004	1
AACGACG	30	2.160763E-6	45.000004	1
CAAGCCG	35	1.208391E-7	45.000004	1
TAATCAG	25	3.8847196E-5	45.0	1
TCGCCCG	20	7.0259423E-4	45.0	1
CGGGTGA	20	7.0259423E-4	45.0	6
CAGCGGT	20	7.0259423E-4	45.0	27
CAGCGCG	20	7.0259423E-4	45.0	27
TTGGCGC	20	7.0259423E-4	45.0	27
ACACGTG	45	3.8380676E-10	45.0	42
AGTCCAA	20	7.0259423E-4	45.0	27
ATCTACT	20	7.0259423E-4	45.0	6
AGTCAAC	20	7.0259423E-4	45.0	17
TTTACGG	25	3.8847196E-5	45.0	2
ACATTTA	20	7.0259423E-4	45.0	20
CCTCGGT	20	7.0259423E-4	45.0	13
ATATCGA	20	7.0259423E-4	45.0	42
ACTAGAG	25	3.8847196E-5	45.0	1
GAGGTAT	25	3.8847196E-5	45.0	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content