##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552728_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	329129
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32203482525089	31.0	31.0	34.0	30.0	34.0
2	31.487103840743295	31.0	31.0	34.0	28.0	34.0
3	31.48003366461175	31.0	31.0	34.0	28.0	34.0
4	35.19300942791428	37.0	35.0	37.0	32.0	37.0
5	35.234895739968216	37.0	35.0	37.0	33.0	37.0
6	35.26204618857651	37.0	35.0	37.0	32.0	37.0
7	35.72222441656615	37.0	35.0	37.0	35.0	37.0
8	35.74121089299332	37.0	35.0	37.0	35.0	37.0
9	37.45143089791541	39.0	37.0	39.0	35.0	39.0
10	36.87930872089667	39.0	37.0	39.0	33.0	39.0
11	36.697492472556355	39.0	35.0	39.0	32.0	39.0
12	36.584861862673904	39.0	35.0	39.0	32.0	39.0
13	36.495276927891496	39.0	35.0	39.0	32.0	39.0
14	37.42217489191168	39.0	36.0	40.0	32.0	41.0
15	37.43780402213114	39.0	36.0	40.0	32.0	41.0
16	37.4851471611435	39.0	36.0	40.0	32.0	41.0
17	37.468308778624795	39.0	36.0	40.0	32.0	41.0
18	37.41386508025729	39.0	36.0	40.0	32.0	41.0
19	37.35147920724093	39.0	36.0	40.0	32.0	41.0
20	37.204825463572035	39.0	35.0	40.0	32.0	41.0
21	37.15035745862564	39.0	35.0	40.0	32.0	41.0
22	37.14360934466425	39.0	35.0	40.0	32.0	41.0
23	37.0817156798702	39.0	35.0	40.0	32.0	41.0
24	37.062100270714524	39.0	35.0	40.0	32.0	41.0
25	37.0328138814872	39.0	35.0	40.0	32.0	41.0
26	36.98186729215596	39.0	35.0	40.0	31.0	41.0
27	36.88590795706243	39.0	35.0	40.0	31.0	41.0
28	36.826609019563755	39.0	35.0	40.0	31.0	41.0
29	36.75084845151901	39.0	35.0	40.0	31.0	41.0
30	36.59860723303021	39.0	35.0	40.0	31.0	41.0
31	36.45587292520562	38.0	35.0	40.0	30.0	41.0
32	36.310720112782526	38.0	35.0	40.0	30.0	41.0
33	36.12168784883739	38.0	35.0	40.0	30.0	41.0
34	35.95193677858833	38.0	35.0	40.0	29.0	41.0
35	35.88130186036478	38.0	35.0	40.0	29.0	41.0
36	35.74831449067083	38.0	35.0	40.0	28.0	41.0
37	35.580270957588056	38.0	35.0	40.0	27.0	41.0
38	35.54011041263456	38.0	35.0	40.0	27.0	41.0
39	35.51145295613574	38.0	35.0	40.0	27.0	41.0
40	35.37126780077113	38.0	34.0	40.0	26.0	41.0
41	35.3339754321254	38.0	34.0	40.0	26.0	41.0
42	35.25314694238429	38.0	34.0	40.0	26.0	41.0
43	35.09111928757417	38.0	34.0	40.0	25.0	41.0
44	34.955904827590395	38.0	34.0	40.0	25.0	41.0
45	34.9761248628957	38.0	34.0	40.0	25.0	41.0
46	34.83239702365942	38.0	34.0	40.0	24.0	41.0
47	34.703022219251416	38.0	33.0	40.0	24.0	41.0
48	34.648645363975824	38.0	33.0	40.0	24.0	41.0
49	34.60019931394681	37.0	33.0	40.0	24.0	41.0
50	34.416751486499216	37.0	33.0	40.0	23.0	41.0
51	33.057946884048505	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	18.0
10	20.0
11	18.0
12	5.0
13	5.0
14	7.0
15	9.0
16	35.0
17	98.0
18	193.0
19	324.0
20	513.0
21	868.0
22	1255.0
23	1813.0
24	2642.0
25	3368.0
26	4021.0
27	4651.0
28	5255.0
29	6157.0
30	7791.0
31	9491.0
32	12209.0
33	16187.0
34	23705.0
35	27774.0
36	31067.0
37	41920.0
38	63466.0
39	64232.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.429652203239456	21.622828738883538	24.453026017154368	12.494493040722634
2	33.0156260918971	24.33635443853322	24.062297761667917	18.585721707901765
3	29.707804538645945	24.91363568691911	29.47081539457173	15.907744379863214
4	28.62555411404039	25.592396902126524	27.561533623594396	18.22051536023869
5	22.919584722099845	28.46695368685227	27.240382950150245	21.373078640897642
6	23.561582236752155	32.706324875656655	27.765101221709422	15.966991665881766
7	72.17230933767611	6.986622266649246	13.974459862242464	6.86660853343218
8	68.77151512021122	6.186328157044806	14.88504507351221	10.15711164923176
9	63.041542981627266	8.184632773167968	18.619143253860948	10.15468099134382
10	33.37171747248039	24.39651322125975	24.892063598163638	17.33970570809622
11	23.90308967000781	26.29668002515731	28.130915233844476	21.669315070990404
12	23.00283475476181	20.872059283745887	36.50027800649593	19.62482795499637
13	22.803824640186672	22.74761567652805	36.67558920666365	17.772970476621627
14	16.684643407296225	29.20830434267415	31.639266062850734	22.467786187178888
15	14.722798659492176	25.984644318792938	39.23142597583318	20.061131045881705
16	15.522788936860623	27.247978756050063	33.10282594362697	24.12640636346235
17	15.881614807567853	26.159044022252676	30.378666115717547	27.580675054461928
18	16.746017518966728	25.51795800430834	34.86201459002397	22.87400988670096
19	17.208450182147423	26.93320855956175	32.15608469627411	23.702256562016714
20	20.280194088032353	27.48192957776434	31.68028341470973	20.557592919493572
21	19.386319649742198	28.280096861716835	33.073050384499695	19.260533104041272
22	15.099854464358959	27.61986941290497	28.077744592545777	29.20253153019029
23	16.40876373701497	27.4755491008085	30.76787521002403	25.347811952152497
24	19.95053611198041	23.99059335397367	29.876431429621817	26.1824391044241
25	15.728179528391603	27.73228734022222	28.63831506795208	27.901218063434097
26	16.609293012770067	26.448292310917605	33.26294553199505	23.679469144317274
27	22.01811447790988	27.408098344418146	29.116547007404392	21.457240170267585
28	15.414016996375283	27.688535498239293	30.698601460217727	26.198846045167702
29	18.23540313980233	25.177969732232654	30.26746351734426	26.31916361062076
30	23.265953471131382	27.345812736039672	25.450811080153983	23.93742271267497
31	17.29352320822535	31.832503364942017	23.85478034448499	27.019193082347652
32	19.449820587064647	26.92591658589793	26.536099827119457	27.088162999917962
33	23.013468883021552	27.6800281956315	23.75694636449538	25.54955655685157
34	16.975714689377114	24.267384520962906	30.454928006951683	28.3019727827083
35	19.547350734818263	20.755083872888747	30.38109677360549	29.31646861868751
36	24.808813565501673	25.48423262611317	28.219026582282325	21.487927226102833
37	21.346037571894303	25.21382193607977	29.32193759893537	24.11820289309055
38	18.957308532520685	28.290730989976577	26.279665419941722	26.47229505756102
39	23.446429819310968	23.050536415812644	26.660367211640423	26.842666553235965
40	22.079184757344382	21.01759492478633	32.08377262410787	24.81944769376141
41	20.83256109306685	21.726435531357005	28.790231185948368	28.650772189627776
42	24.915458680335067	20.084526128053135	31.810931276186537	23.18908391542526
43	26.56800221189868	20.442136669816392	29.861543650058188	23.128317468226744
44	20.2455572131292	23.217947977844553	27.5123128013636	29.024182007662645
45	20.722573823637543	19.0751954400858	28.174059411355422	32.02817132492123
46	22.949056448991126	21.701217455769623	30.47589243123517	24.87383366400408
47	17.858347334935544	20.874793773869822	35.21051016470745	26.056348726487183
48	19.241999337645726	18.912645193829775	33.436433738746814	28.408921729777685
49	23.255927007343622	17.172901810536295	34.625936942657745	24.945234239462337
50	20.414487936341068	16.46649186185356	33.37414813036833	29.744872071437033
51	18.791112299432744	16.521485496568214	28.341774805623327	36.34562739837571
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	395.0
1	442.0
2	489.0
3	1396.5
4	2304.0
5	1678.5
6	1053.0
7	1115.5
8	1178.0
9	1194.0
10	1210.0
11	1262.0
12	1314.0
13	1339.5
14	1365.0
15	1336.0
16	1307.0
17	1384.5
18	1462.0
19	1512.5
20	1563.0
21	1638.5
22	1714.0
23	1689.0
24	1664.0
25	1848.0
26	1975.5
27	1919.0
28	2470.5
29	3022.0
30	3198.0
31	3374.0
32	3733.5
33	4093.0
34	4270.0
35	4447.0
36	5116.0
37	5785.0
38	6506.5
39	7228.0
40	8404.5
41	9581.0
42	12111.5
43	14642.0
44	18640.5
45	22639.0
46	30767.0
47	38895.0
48	46148.5
49	53402.0
50	52196.5
51	50991.0
52	40580.0
53	30169.0
54	24147.0
55	18125.0
56	14775.0
57	11425.0
58	9758.0
59	8091.0
60	7230.5
61	6370.0
62	5899.5
63	5429.0
64	4538.5
65	3648.0
66	2954.5
67	2261.0
68	1873.0
69	1485.0
70	1272.0
71	1059.0
72	917.0
73	775.0
74	692.5
75	467.0
76	324.0
77	232.0
78	140.0
79	111.0
80	82.0
81	61.0
82	40.0
83	28.0
84	16.0
85	10.5
86	5.0
87	4.0
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	329129.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.206734820474374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.33716841785846	26.79277240541073
2	13.301644682292398	10.430281114696225
3	5.812455580653648	6.836622138194219
4	3.073207295145448	4.819616938764596
5	1.9332862787784257	3.7898921232063723
6	1.3304991894762084	3.129871734038988
7	0.9778889904575807	2.6837884032912234
8	0.7385311416331505	2.3164315701338505
9	0.6258932068274863	2.2085306087419423
>10	3.7086846990224	24.33073468425941
>50	0.0900768119379478	2.3597903992152407
>100	0.06056889078586145	4.786255304613959
>500	0.005435669685910644	1.3779397944585445
>1k	0.004659145445066266	4.137472780974728
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	3984	1.2104676281944162	TruSeq Adapter, Index 19 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGCT	2948	0.8956974317061093	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	2602	0.7905714780526785	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1732	0.5262374327391388	TruSeq Adapter, Index 19 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1178	0.3579143739992526	No Hit
ACTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTGC	1146	0.3481917424474902	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCC	866	0.2631187163695694	No Hit
GCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	747	0.22696268028645303	TruSeq Adapter, Index 19 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	640	0.19445263103524757	TruSeq Adapter, Index 13 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	614	0.18655299289944063	No Hit
CGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	596	0.1810840126515743	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	555	0.16862689097587874	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG	508	0.15434677588422777	No Hit
GGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	492	0.14948546010834657	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGC	465	0.14128198973654707	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCT	382	0.1160639141491634	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	367	0.11150643060927477	No Hit
TGCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTCTG	364	0.11059493390129707	TruSeq Adapter, Index 13 (95% over 21bp)
AGGCCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCGTCTTC	346	0.10512595365343072	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTGACACTGTCGTATGCCG	337	0.10239146352949756	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9756053097721562	0.0
2	0.0	0.0	0.0	4.09657003788788	0.0
3	0.0	0.0	0.0	5.580486678475613	0.0
4	0.0	0.0	0.0	7.1907975292362565	0.0
5	0.0	0.0	0.0	11.405862139161242	0.0
6	0.0	0.0	0.0	13.490758942542286	0.0
7	0.0	0.0	0.0	15.78195783416229	0.0
8	0.0	0.0	0.0	19.393003958934035	0.0
9	0.0	0.0	0.0	20.67153000799079	0.0
10	0.0	0.0	0.0	23.784291265734712	0.0
11	0.0	0.0	0.0	28.281312190660806	0.0
12	0.0	0.0	0.0	31.652027016762425	0.0
13	0.0	0.0	0.0	32.96306311507038	0.0
14	0.0	0.0	0.0	33.43066092626295	0.0
15	0.0	0.0	0.0	34.33365033163289	0.0
16	0.0	0.0	0.0	36.38178343445883	0.0
17	0.0	0.0	0.0	38.71673416806176	0.0
18	0.0	0.0	0.0	41.79789687326246	0.0
19	0.0	0.0	0.0	43.244442148823104	0.0
20	0.0	0.0	0.0	44.60196457923793	0.0
21	0.002734490123933169	0.0	0.0	46.036660397594865	0.0
22	0.002734490123933169	0.0	0.0	47.415147252293174	0.0
23	0.0030383223599257433	0.0	0.0	48.681823844146216	0.0
24	0.003645986831910892	0.0	0.0	49.67170926901002	0.0
25	0.003949819067903466	0.0	0.0	50.54583461196066	0.0
26	0.003949819067903466	0.0	0.0	51.345521057093116	0.0
27	0.003949819067903466	0.0	0.0	52.04889268341592	0.0
28	0.003949819067903466	0.0	0.0	52.813334589173245	0.0
29	0.003949819067903466	0.0	0.0	53.56015422524299	0.0
30	0.003949819067903466	0.0	0.0	54.41635346627007	0.0
31	0.003949819067903466	0.0	0.0	55.27194504282515	0.0
32	0.003949819067903466	0.0	0.0	56.02605665255872	0.0
33	0.003949819067903466	0.0	0.0	56.77439544980843	0.0
34	0.003949819067903466	0.0	0.0	57.42945775060842	0.0
35	0.003949819067903466	0.0	0.0	58.11824542960359	0.0
36	0.003949819067903466	0.0	0.0	58.82951669406221	0.0
37	0.003949819067903466	0.0	0.0	59.51951970200134	0.0
38	0.003949819067903466	0.0	0.0	60.188254453420996	0.0
39	0.004253651303896041	0.0	0.0	60.831163464781284	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTACGG	35	1.208391E-7	45.000004	2
AGTGCAG	30	2.160763E-6	45.000004	1
AACGACG	30	2.160763E-6	45.000004	1
CAAGCCG	35	1.208391E-7	45.000004	1
TAATCAG	25	3.8847196E-5	45.0	1
TCGCCCG	20	7.0259423E-4	45.0	1
CGGGTGA	20	7.0259423E-4	45.0	6
CAGCGGT	20	7.0259423E-4	45.0	27
CAGCGCG	20	7.0259423E-4	45.0	27
TTGGCGC	20	7.0259423E-4	45.0	27
ACACGTG	45	3.8380676E-10	45.0	42
AGTCCAA	20	7.0259423E-4	45.0	27
ATCTACT	20	7.0259423E-4	45.0	6
AGTCAAC	20	7.0259423E-4	45.0	17
TTTACGG	25	3.8847196E-5	45.0	2
ACATTTA	20	7.0259423E-4	45.0	20
CCTCGGT	20	7.0259423E-4	45.0	13
ATATCGA	20	7.0259423E-4	45.0	42
ACTAGAG	25	3.8847196E-5	45.0	1
GAGGTAT	25	3.8847196E-5	45.0	9
>>END_MODULE