##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552726_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1322724
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70936642867295	31.0	31.0	34.0	30.0	34.0
2	31.868813902219966	31.0	31.0	34.0	30.0	34.0
3	31.925611843438237	33.0	31.0	34.0	30.0	34.0
4	35.544030349490896	37.0	35.0	37.0	33.0	37.0
5	35.53333272852084	37.0	35.0	37.0	33.0	37.0
6	35.55814667307768	37.0	35.0	37.0	33.0	37.0
7	35.95187658196268	37.0	35.0	37.0	35.0	37.0
8	35.975442344737075	37.0	35.0	37.0	35.0	37.0
9	37.71640569007594	39.0	37.0	39.0	35.0	39.0
10	37.08428440097859	39.0	37.0	39.0	33.0	39.0
11	36.88646459881275	39.0	37.0	39.0	33.0	39.0
12	36.77091063593009	39.0	35.0	39.0	33.0	39.0
13	36.75527623298587	39.0	35.0	39.0	33.0	39.0
14	37.84233596729174	40.0	37.0	41.0	33.0	41.0
15	37.850653651101815	40.0	37.0	41.0	33.0	41.0
16	37.850221210169316	40.0	37.0	41.0	33.0	41.0
17	37.830180748213536	40.0	37.0	41.0	33.0	41.0
18	37.8116629017089	40.0	36.0	41.0	33.0	41.0
19	37.82457413640336	40.0	37.0	41.0	33.0	41.0
20	37.74074107674768	39.0	36.0	41.0	33.0	41.0
21	37.70351713584996	39.0	36.0	41.0	33.0	41.0
22	37.69339106268579	39.0	36.0	41.0	33.0	41.0
23	37.64023409267542	39.0	36.0	41.0	33.0	41.0
24	37.657819015909595	39.0	36.0	41.0	33.0	41.0
25	37.6041214947336	39.0	36.0	41.0	32.0	41.0
26	37.54306945364264	39.0	36.0	41.0	32.0	41.0
27	37.477998433535646	39.0	36.0	41.0	32.0	41.0
28	37.45291610343503	39.0	36.0	41.0	32.0	41.0
29	37.42743610912027	39.0	36.0	41.0	32.0	41.0
30	37.33487938526858	39.0	36.0	41.0	32.0	41.0
31	37.256181939694144	39.0	36.0	41.0	32.0	41.0
32	37.18322718874081	39.0	36.0	41.0	31.0	41.0
33	37.10159640257529	39.0	35.0	41.0	31.0	41.0
34	37.01102346370067	39.0	35.0	41.0	31.0	41.0
35	36.950927782364275	39.0	35.0	41.0	31.0	41.0
36	36.88478624414466	39.0	35.0	41.0	31.0	41.0
37	36.759681535981805	39.0	35.0	40.0	31.0	41.0
38	36.71602239015849	39.0	35.0	40.0	31.0	41.0
39	36.684479150601334	39.0	35.0	40.0	30.0	41.0
40	36.55660818129859	39.0	35.0	40.0	30.0	41.0
41	36.496731744490916	39.0	35.0	40.0	30.0	41.0
42	36.42578723906121	39.0	35.0	40.0	30.0	41.0
43	36.313243730362494	38.0	35.0	40.0	30.0	41.0
44	36.22736186838675	38.0	35.0	40.0	30.0	41.0
45	36.18939400812263	38.0	35.0	40.0	30.0	41.0
46	36.06818731647721	38.0	35.0	40.0	30.0	41.0
47	35.97762798588368	38.0	35.0	40.0	29.0	41.0
48	35.88186499980343	38.0	35.0	40.0	29.0	41.0
49	35.79491715580877	38.0	34.0	40.0	29.0	41.0
50	35.66985100444235	38.0	34.0	40.0	28.0	41.0
51	34.44740626162374	37.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	44.0
10	80.0
11	64.0
12	38.0
13	23.0
14	25.0
15	32.0
16	66.0
17	129.0
18	289.0
19	481.0
20	866.0
21	1471.0
22	2172.0
23	3344.0
24	4929.0
25	6961.0
26	9000.0
27	11903.0
28	15151.0
29	19260.0
30	24983.0
31	32623.0
32	41937.0
33	56474.0
34	87843.0
35	105007.0
36	117470.0
37	167807.0
38	270191.0
39	342003.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.04645262352539	20.652985808074853	27.12289185045406	8.177669717945694
2	31.540517900937765	23.577934625817633	26.77640989352276	18.10513757972185
3	32.5850290763606	24.127935986645742	26.79394945581996	16.4930854811737
4	28.394510117000976	26.641083098212476	26.165020064654453	18.79938672013209
5	26.315013562920154	30.600866091489987	24.198547845204292	18.885572500385567
6	26.966396617888538	36.242330221573056	22.358405835230933	14.432867325307472
7	84.61583822475437	5.108624323744031	6.102180046631043	4.173357404870555
8	85.38531091898234	4.088305648041466	6.042606016069868	4.483777416906324
9	79.28479410670707	7.1661208233917275	8.30709959144916	5.241985478452043
10	38.447400969514426	34.20214647953768	14.31243403763748	13.038018513310412
11	27.648851914685153	26.818519963348365	26.73891151895634	18.793716603010154
12	26.061521526788656	24.175338165785153	29.84182641276638	19.92131389465981
13	24.96348444573471	24.736982167103644	30.914461369114036	19.385072018047605
14	20.994326858815597	28.868153900586968	28.966965141631967	21.170554098965468
15	20.24458617217197	28.12166408109326	31.4801122531987	20.153637493536067
16	23.542099485606975	27.49318829929751	28.79194752646811	20.17276468862741
17	22.890867633761843	26.014497355457372	28.390503234234803	22.704131776545978
18	23.468917173953145	26.296566781883445	29.453083182886225	20.78143286127718
19	23.1319610137867	27.84806202956928	27.935230630123897	21.084746326520122
20	24.866260837483857	27.89614462276333	27.77934021005138	19.458254329701436
21	23.986485464843764	27.755298913454357	28.622297622179683	19.6359179995222
22	22.66368494107614	25.296584926258237	27.669339937885756	24.370390194779866
23	21.009447171140767	27.820391858014222	28.57413942742401	22.596021543421003
24	22.31977343724012	26.15035336169904	28.819012885530164	22.710860315530677
25	21.282368808610112	28.25797369670468	27.126671928535355	23.332985566149855
26	20.754972314708134	28.351341625312614	28.631747817382912	22.261938242596337
27	22.019862042270343	28.26175377478597	27.969100129732276	21.749284053211404
28	19.924186754001592	27.945436840943387	28.440022257099745	23.69035414795528
29	21.128141622893363	26.46825792833577	28.440249061784623	23.96335138698625
30	23.012359343294595	27.7481167650999	27.217393802486384	22.02213008911912
31	23.547164790235907	29.004312313075136	24.78211629939428	22.666406597294674
32	22.355457374327525	29.24994178679755	25.96747318412609	22.427127654748837
33	22.971685703139887	29.24517888841512	25.048460601002176	22.734674807442822
34	22.553760270472146	26.62407274684666	27.88246074010905	22.939706242572147
35	20.672037401604566	28.250564743665347	27.306754848328147	23.77064300640194
36	22.86123182160451	30.637230442632024	25.956510957690345	20.54502677807313
37	24.05475367499191	29.055343367172593	25.556200688881432	21.333702268954067
38	22.3664952023249	29.512203604077648	24.409627405263684	23.711673788333773
39	24.087489151175905	26.374058382549947	25.870325177436865	23.66812728883728
40	23.701921186884036	26.623997145285035	28.11228948745165	21.56179218037928
41	21.98372449581319	28.27105276686595	26.672079738479077	23.073142998841785
42	23.72732331159032	27.00578503149561	27.13098121754803	22.135910439366036
43	23.71575627266157	25.71307392925508	27.591621532534376	22.979548265548974
44	22.31576655447395	26.040806698903175	26.491165201508405	25.152261545114474
45	23.14934937296065	24.983065250195807	26.41034713213036	25.45723824471318
46	23.281198496436144	26.801887619790676	26.79810754170938	23.118806342063802
47	21.532987985399828	26.951956719617996	28.835494025964604	22.679561269017572
48	22.10045330696351	25.970724051276	28.082729276855943	23.846093364904544
49	23.80783897472186	24.874350204577826	28.3434034613419	22.974407359358416
50	21.818383880537436	24.542232544355436	27.9799867546064	25.659396820500724
51	21.444912166105702	24.161654283130872	26.242284860636083	28.151148690127343
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	233.0
1	531.5
2	830.0
3	1299.0
4	1768.0
5	1380.0
6	992.0
7	1011.5
8	1031.0
9	1158.0
10	1285.0
11	1382.5
12	1480.0
13	1638.5
14	1797.0
15	1786.0
16	1775.0
17	1996.0
18	2217.0
19	2531.5
20	2846.0
21	3140.5
22	3435.0
23	4315.5
24	5196.0
25	6429.0
26	9077.0
27	10492.0
28	13101.5
29	15711.0
30	18594.5
31	21478.0
32	24935.0
33	28392.0
34	32765.5
35	37139.0
36	39967.5
37	42796.0
38	47323.0
39	51850.0
40	56371.0
41	60892.0
42	68852.0
43	76812.0
44	85119.0
45	93426.0
46	106193.5
47	118961.0
48	136469.5
49	153978.0
50	149747.5
51	145517.0
52	128066.0
53	110615.0
54	94488.5
55	78362.0
56	68603.5
57	58845.0
58	53422.5
59	48000.0
60	44967.0
61	41934.0
62	38165.0
63	34396.0
64	28301.5
65	22207.0
66	18300.0
67	14393.0
68	11697.0
69	9001.0
70	7529.5
71	6058.0
72	4860.5
73	3663.0
74	3089.5
75	1977.5
76	1439.0
77	1014.0
78	589.0
79	481.0
80	373.0
81	261.0
82	149.0
83	133.5
84	118.0
85	70.5
86	23.0
87	22.5
88	22.0
89	14.5
90	7.0
91	8.0
92	9.0
93	8.5
94	8.0
95	7.0
96	6.0
97	3.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1322724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.51093042776003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.4417802062736	19.902696835916238
2	13.124991912638158	7.746614463975557
3	5.324265315491504	4.713720699132168
4	2.708555444432362	3.1972796512149633
5	1.6328328076485539	2.4093207693340277
6	1.0894800547204198	1.92909420583719
7	0.7967040525617178	1.6458034506664325
8	0.6298931575766843	1.4870986520134093
9	0.4816586222840361	1.2792774682939443
>10	6.2339202053037805	42.0184803009106
>50	0.4698046316909621	8.559408205652536
>100	0.06199756829108999	3.4151378480384
>500	0.002572513179492877	0.49930780234408256
>1k	0.0015435079076957263	1.1967596466705364
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	4996	0.3777054018827813	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	3850	0.2910660122595492	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	2281	0.17244716206857968	No Hit
TCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	2195	0.16594542776875598	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5120312325171388E-4	0.0	0.0	0.33869499608383913	0.0
2	1.5120312325171388E-4	0.0	0.0	1.2840925242151802	0.0
3	1.5120312325171388E-4	0.0	0.0	1.7488909250909488	0.0
4	1.5120312325171388E-4	0.0	0.0	2.297229051563289	0.0
5	1.5120312325171388E-4	0.0	0.0	3.7737275501162753	0.0
6	1.5120312325171388E-4	0.0	0.0	4.5083479244347275	0.0
7	1.5120312325171388E-4	0.0	0.0	5.356597445876842	0.0
8	1.5120312325171388E-4	0.0	0.0	6.8201680773918065	0.0
9	1.5120312325171388E-4	0.0	0.0	7.318306766944578	0.0
10	1.5120312325171388E-4	0.0	0.0	8.695011204151433	0.0
11	1.5120312325171388E-4	0.0	0.0	10.76891324267194	0.0
12	1.5120312325171388E-4	0.0	0.0	12.264992545686024	0.0
13	1.5120312325171388E-4	0.0	0.0	12.858691609133878	0.0
14	1.5120312325171388E-4	0.0	0.0	13.087235129928844	0.0
15	1.5120312325171388E-4	0.0	0.0	13.558535265104435	0.0
16	1.5120312325171388E-4	0.0	0.0	14.596469104665825	0.0
17	1.5120312325171388E-4	0.0	0.0	15.955104768644102	0.0
18	1.5120312325171388E-4	0.0	0.0	17.352297229051562	0.0
19	1.5120312325171388E-4	0.0	0.0	18.23721350788222	0.0
20	1.5120312325171388E-4	0.0	0.0	19.058246467139025	0.0
21	4.5360936975514165E-4	0.0	0.0	20.085520486511168	0.0
22	4.5360936975514165E-4	0.0	0.0	21.21009371569579	0.0
23	6.048124930068555E-4	0.0	0.0	22.28454310952247	0.0
24	7.560156162585694E-4	0.0	0.0	23.145569294879355	0.0
25	0.0011340234243878541	0.0	0.0	23.8795848567048	0.0
26	0.0011340234243878541	0.0	0.0	24.577840879881215	0.0
27	0.0011340234243878541	0.0	0.0	25.272090020291458	0.0
28	0.0011340234243878541	0.0	0.0	25.985163949546543	0.0
29	0.0011340234243878541	0.0	0.0	26.722657183206778	0.0
30	0.0011340234243878541	0.0	0.0	27.558432446980625	0.0
31	0.0011340234243878541	0.0	0.0	28.294338047846715	0.0
32	0.0011340234243878541	0.0	0.0	29.022834695673474	0.0
33	0.0015876327941429958	0.0	0.0	29.707104429949105	0.0
34	0.0016632343557688527	0.0	0.0	30.4301577653388	0.0
35	0.0020412421638981377	0.0	0.0	31.166138967766518	0.0
36	0.0021168437255239944	0.0	0.0	31.88246376417151	0.0
37	0.0021168437255239944	0.0	0.0	32.59591570123472	0.0
38	0.0021168437255239944	0.0	0.0	33.33068727867643	0.0
39	0.0021168437255239944	0.0	0.0	34.068482918583165	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTACG	20	7.033674E-4	45.0	1
CATCGCA	20	7.033674E-4	45.0	14
CGTTAGG	175	0.0	42.42857	2
TACGGGT	65	0.0	41.53846	4
TAATGCG	60	3.6379788E-12	41.250004	1
TCGGCGA	45	1.9286745E-8	40.0	24
AATTGCG	45	1.9286745E-8	40.0	1
TTAACGG	90	0.0	40.0	2
TGTTGCG	130	0.0	39.80769	1
TCGTACA	40	3.4587538E-7	39.375	34
GTACGTA	40	3.4587538E-7	39.375	8
CCCGATT	40	3.4587538E-7	39.375	42
CGACCAA	320	0.0	37.96875	29
GCGTTAG	120	0.0	37.500004	1
ATACTCG	30	1.1400149E-4	37.500004	44
CGTTAGC	30	1.1400149E-4	37.500004	45
CTCGATC	30	1.1400149E-4	37.500004	16
AAGATCG	30	1.1400149E-4	37.500004	33
GCGATAC	90	0.0	37.5	9
CACGACC	325	0.0	37.384617	27
>>END_MODULE