FastQCFastQC Report
Sat 18 Jun 2016
SRR3552723_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552723_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451454
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC36760.8142579310405932TruSeq Adapter, Index 21 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC32140.7119219233853282TruSeq Adapter, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT28050.6213257607641088TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC17770.3936170684056405TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.2824208003473222No Hit
ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC11160.24720126524518554TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC10900.24144209598320093No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG8790.19470422235709509No Hit
GCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG6750.1495168943015235TruSeq Adapter, Index 21 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG6530.14464375107984426No Hit
GGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG6400.14176416644885195TruSeq Adapter, Index 21 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG5920.13113185396518803No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTC5210.11540489174976852No Hit
GGCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT5110.11318982664900522No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTC207.02873E-445.06
AACCGAA207.02873E-445.039
CTCGTTC207.02873E-445.045
TTTACGG406.7993824E-945.02
CGCGGGT207.02873E-445.04
CGTGCGG253.8870297E-545.02
TAGACGG253.8870297E-545.02
TACGGGT406.7993824E-945.04
ACGGGTC207.02873E-445.05
GTAATCG253.8870297E-545.01
TAACCGA207.02873E-445.038
TCGTGCA207.02873E-445.022
TACTGCG253.8870297E-545.01
CGCTAAT207.02873E-445.015
GTGTACG253.8870297E-545.01
TTGAACG207.02873E-445.01
GAACGAA207.02873E-445.09
GCGATAT253.8870297E-545.09
GCATGCG207.02873E-445.01
CGATCCA207.02873E-445.010
Produced by FastQC (version 0.11.5)