##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552723_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 451454 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.445135052519195 31.0 31.0 34.0 30.0 34.0 2 31.607738551436025 31.0 31.0 34.0 30.0 34.0 3 31.576625747030704 31.0 31.0 34.0 30.0 34.0 4 35.285169696137366 37.0 35.0 37.0 33.0 37.0 5 35.32416813230141 37.0 35.0 37.0 33.0 37.0 6 35.36144324781705 37.0 35.0 37.0 33.0 37.0 7 35.837312328609336 37.0 35.0 37.0 35.0 37.0 8 35.87597185981296 37.0 35.0 37.0 35.0 37.0 9 37.60056395557466 39.0 37.0 39.0 35.0 39.0 10 36.93775002547325 39.0 37.0 39.0 33.0 39.0 11 36.767050463613124 39.0 37.0 39.0 32.0 39.0 12 36.70208924940304 39.0 35.0 39.0 32.0 39.0 13 36.66880789626407 39.0 35.0 39.0 32.0 39.0 14 37.67947121965915 39.0 37.0 41.0 33.0 41.0 15 37.65126901079623 39.0 36.0 41.0 33.0 41.0 16 37.67881999051952 39.0 36.0 41.0 33.0 41.0 17 37.659101480992526 39.0 36.0 41.0 33.0 41.0 18 37.6076565940273 39.0 36.0 41.0 33.0 41.0 19 37.56916983790153 39.0 36.0 40.0 32.0 41.0 20 37.43589823104901 39.0 36.0 40.0 32.0 41.0 21 37.40350290395035 39.0 36.0 40.0 32.0 41.0 22 37.41582752617099 39.0 36.0 40.0 32.0 41.0 23 37.386147868885864 39.0 36.0 40.0 32.0 41.0 24 37.372799886588666 39.0 36.0 41.0 32.0 41.0 25 37.337533835119416 39.0 36.0 40.0 32.0 41.0 26 37.29261896007124 39.0 36.0 40.0 32.0 41.0 27 37.24854802482645 39.0 35.0 40.0 32.0 41.0 28 37.180217696598106 39.0 35.0 40.0 32.0 41.0 29 37.03775799970761 39.0 35.0 40.0 31.0 41.0 30 36.94323009653254 39.0 35.0 40.0 31.0 41.0 31 36.75726652106305 39.0 35.0 40.0 31.0 41.0 32 36.35506386032686 38.0 35.0 40.0 30.0 41.0 33 36.26032995609741 38.0 35.0 40.0 30.0 41.0 34 36.104976365255375 38.0 35.0 40.0 30.0 41.0 35 35.84012989141751 38.0 35.0 40.0 29.0 41.0 36 35.86074771737541 38.0 35.0 40.0 29.0 41.0 37 35.70232404630372 38.0 35.0 40.0 28.0 41.0 38 35.51685000022151 38.0 34.0 40.0 27.0 41.0 39 35.55438870848414 38.0 34.0 40.0 27.0 41.0 40 35.50013290390604 38.0 34.0 40.0 27.0 41.0 41 35.39105866821426 38.0 34.0 40.0 27.0 41.0 42 35.38056811989704 38.0 34.0 40.0 27.0 41.0 43 35.34701874388088 38.0 34.0 40.0 27.0 41.0 44 35.27583984193296 38.0 34.0 40.0 26.0 41.0 45 35.21883735662991 38.0 34.0 40.0 26.0 41.0 46 35.00796758916744 38.0 34.0 40.0 25.0 41.0 47 34.800617560150094 38.0 33.0 40.0 24.0 41.0 48 34.688355845778304 38.0 33.0 40.0 24.0 41.0 49 34.67372312572267 38.0 33.0 40.0 24.0 41.0 50 34.500163914817456 37.0 33.0 40.0 24.0 41.0 51 32.94434205921312 36.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 4.0 9 15.0 10 24.0 11 24.0 12 16.0 13 8.0 14 9.0 15 20.0 16 30.0 17 59.0 18 147.0 19 278.0 20 436.0 21 698.0 22 1156.0 23 1870.0 24 2682.0 25 4032.0 26 5355.0 27 5972.0 28 6767.0 29 8194.0 30 10437.0 31 12747.0 32 16696.0 33 21796.0 34 31520.0 35 37296.0 36 44028.0 37 61724.0 38 94101.0 39 83301.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.95101605036172 21.216779561151302 25.718899378452733 12.113305010034244 2 34.38888568935041 22.914183947866228 24.359735432624365 18.337194930158997 3 30.379839363478894 24.359292419604213 28.647879961192057 16.612988255724837 4 27.47588901637819 26.413100781031957 28.022567083246575 18.088443119343278 5 23.746383906223002 29.20098171685266 27.344757162413003 19.707877214511335 6 25.465717437435487 32.55148918826724 27.080278389381863 14.902514984915408 7 76.89022580373637 5.201194363102331 11.966667700363713 5.941912132797583 8 74.88559188754557 3.950125594191213 12.870414261475144 8.293868256788068 9 68.98687352421288 6.83258980981451 15.963531168181033 8.217005497791579 10 34.14965865846797 25.632068826502813 23.90055243723613 16.317720077793084 11 23.41501016714881 24.943626593185574 29.348283546053416 22.293079693612196 12 20.402078617090556 22.28488395273937 36.58556574977739 20.727471680392686 13 21.954839252725638 24.11253416737918 37.127370673424096 16.805255906471093 14 19.23518232200844 27.365800280870257 32.78096993270632 20.61804746441498 15 17.35414903844024 26.23434502740036 36.45354786977189 19.957958064387512 16 18.566011155067848 27.62584892369987 31.770014220717947 22.03812570051434 17 18.35092833378373 26.771498314335457 30.55039937623767 24.32717397564314 18 19.418368205841567 24.769743982775655 35.410030700802295 20.40185711058048 19 18.351371346803884 27.050153504011483 32.265745790268774 22.33272935891586 20 19.650950041421716 28.174077536138785 32.322894469868466 19.852077952571026 21 19.023422098375473 28.243630580302753 33.732118886974085 19.000828434347685 22 17.74466501570481 25.90496484691685 30.109822927695845 26.24054720968249 23 16.875916483185442 27.06366540112614 31.354246501304672 24.706171614383745 24 20.091304983453465 24.446122971554136 31.215140412976737 24.247431632015665 25 16.60545703438224 27.75255064746353 29.582415927204103 26.059576390950127 26 16.782219229423152 26.432371847408596 33.55956531562463 23.225843607543624 27 20.261421983192086 25.278987449441136 30.821523344571094 23.638067222795677 28 17.091220810979635 24.286195271279023 32.19043357684283 26.43215034089852 29 18.6703407213138 22.34668426905067 34.108458447593776 24.87451656204176 30 23.879952331799032 22.542274517448067 29.118581295104264 24.459191855648637 31 23.767648531190332 25.47834330850984 27.416525271677735 23.337482888622098 32 20.166617196879415 24.39207538309551 32.37096138255504 23.070346037470042 33 23.070124530959966 24.147975208991394 27.94260323310902 24.839297026939622 34 23.06813097236928 21.38645354786977 32.2785931678532 23.26682231190775 35 19.221227411873635 21.648938762310223 34.412586885928576 24.717246939887563 36 23.779609882734455 24.919039370567102 29.15269329765602 22.148657449042428 37 23.28476433922393 23.56652062004102 31.879438436695658 21.26927660403939 38 21.85826241433236 24.82113349311336 31.34073460419002 21.979869488364262 39 25.770731901810596 22.63885135584135 30.82750402034316 20.7629127220049 40 26.272887160153637 20.032827264793312 32.933587918148916 20.760697656904135 41 21.039574353090238 23.534402176079954 30.021663336685467 25.404360134144344 42 21.44027962981832 23.14100661418439 32.243595139261146 23.175118616736146 43 22.761565962423635 21.913417535341363 31.742990426488632 23.582026075746366 44 22.456551498048526 20.805220465429482 29.78819547506501 26.950032561456982 45 24.723006109149548 17.572554457375503 30.468441967509424 27.23599746596552 46 26.59407159976432 19.151674367709667 31.698910630983445 22.555343401542572 47 20.349138561182315 21.14213186725558 35.1619434095168 23.3467861620453 48 19.76126028343973 20.562892343405974 33.88141427476554 25.794433098388765 49 23.945961271801778 18.10394857504862 35.27956336636734 22.670526786782265 50 22.36152520522578 16.853544325667734 33.94122103248615 26.843709436620344 51 21.34414580444519 16.437998112764536 29.707567105397224 32.510288977393046 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 182.0 1 233.0 2 284.0 3 1420.5 4 2557.0 5 1898.5 6 1240.0 7 1251.5 8 1263.0 9 1337.5 10 1412.0 11 1492.5 12 1573.0 13 1586.0 14 1599.0 15 1595.0 16 1591.0 17 1590.5 18 1590.0 19 1590.5 20 1591.0 21 1582.5 22 1574.0 23 1740.0 24 1906.0 25 2052.0 26 2378.5 27 2559.0 28 3577.0 29 4595.0 30 4857.0 31 5119.0 32 6035.0 33 6951.0 34 7861.5 35 8772.0 36 9695.0 37 10618.0 38 12073.0 39 13528.0 40 15078.5 41 16629.0 42 19257.0 43 21885.0 44 27003.5 45 32122.0 46 41746.0 47 51370.0 48 61783.5 49 72197.0 50 70414.5 51 68632.0 52 55742.0 53 42852.0 54 34757.5 55 26663.0 56 21522.5 57 16382.0 58 13317.0 59 10252.0 60 8568.0 61 6884.0 62 5709.5 63 4535.0 64 3915.0 65 3295.0 66 2629.5 67 1964.0 68 1612.5 69 1261.0 70 1033.0 71 805.0 72 627.0 73 449.0 74 379.5 75 219.0 76 128.0 77 102.0 78 76.0 79 54.0 80 32.0 81 21.5 82 11.0 83 10.0 84 9.0 85 6.5 86 4.0 87 3.5 88 3.0 89 1.5 90 0.0 91 1.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 451454.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.949554592374014 #Duplication Level Percentage of deduplicated Percentage of total 1 69.17368042856884 29.70978763922251 2 13.556030875366027 11.644509762748816 3 5.514140942343503 7.104896922996809 4 2.921706826526416 5.019440273952325 5 1.8772776573553516 4.031411961481384 6 1.2873684886484245 3.3175141910224517 7 0.9885511317655483 2.9720481560782273 8 0.7989697846110273 2.7452317105446893 9 0.6248600277645948 2.4153713887561024 >10 3.133931512389506 20.833303420831935 >50 0.07078275514031794 2.095441544012908 >100 0.045466295272586915 3.8007754702782504 >500 0.0036166371239557777 0.9921337650405218 >1k 0.0036166371239557777 3.3181337930330845 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC 3676 0.8142579310405932 TruSeq Adapter, Index 21 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC 3214 0.7119219233853282 TruSeq Adapter, Index 21 (95% over 22bp) CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT 2805 0.6213257607641088 TruSeq Adapter, Index 15 (95% over 22bp) TCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC 1777 0.3936170684056405 TruSeq Adapter, Index 21 (95% over 22bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1275 0.2824208003473222 No Hit ACTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC 1116 0.24720126524518554 TruSeq Adapter, Index 15 (95% over 21bp) CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCC 1090 0.24144209598320093 No Hit CGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG 879 0.19470422235709509 No Hit GCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG 675 0.1495168943015235 TruSeq Adapter, Index 21 (95% over 21bp) ACCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG 653 0.14464375107984426 No Hit GGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG 640 0.14176416644885195 TruSeq Adapter, Index 21 (95% over 21bp) AGCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTG 592 0.13113185396518803 No Hit CGTTCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTC 521 0.11540489174976852 No Hit GGCCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCT 511 0.11318982664900522 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.6891067528474661 0.0 2 0.0 0.0 0.0 3.1088438689213076 0.0 3 0.0 0.0 0.0 4.339534038905404 0.0 4 0.0 0.0 0.0 5.775339237220182 0.0 5 0.0 0.0 0.0 9.396970676968197 0.0 6 0.0 0.0 0.0 11.215096111674722 0.0 7 0.0 0.0 0.0 13.16523942638674 0.0 8 0.0 0.0 0.0 16.30243612859782 0.0 9 0.0 0.0 0.0 17.381172832669552 0.0 10 0.0 0.0 0.0 19.94245260868217 0.0 11 0.0 0.0 0.0 24.006432549052615 0.0 12 0.0 0.0 0.0 26.871397750379884 0.0 13 0.0 0.0 0.0 28.00595409499085 0.0 14 0.0 0.0 0.0 28.40688087822901 0.0 15 0.0 0.0 0.0 29.25945943551281 0.0 16 0.0 0.0 0.0 31.111918379281168 0.0 17 0.0 0.0 0.0 33.40451075857119 0.0 18 0.0 0.0 0.0 35.914844037266256 0.0 19 0.0 0.0 0.0 37.31011354423706 0.0 20 0.0 0.0 0.0 38.64313972187642 0.0 21 2.2150651007633114E-4 0.0 0.0 40.249947945970135 0.0 22 2.2150651007633114E-4 0.0 0.0 41.8589712351646 0.0 23 8.860260403053246E-4 0.0 0.0 43.45027400355296 0.0 24 0.0011075325503816557 0.0 0.0 44.666344743872024 0.0 25 0.001550545570534318 0.0 0.0 45.72913297921826 0.0 26 0.001550545570534318 0.0 0.0 46.695787389191366 0.0 27 0.001550545570534318 0.0 0.0 47.61725447110891 0.0 28 0.001550545570534318 0.0 0.0 48.57992176390064 0.0 29 0.001550545570534318 0.0 0.0 49.556543966827185 0.0 30 0.001550545570534318 0.0 0.0 50.61800316311297 0.0 31 0.001550545570534318 0.0 0.0 51.53326806274836 0.0 32 0.001550545570534318 0.0 0.0 52.44919748191399 0.0 33 0.001772052080610649 0.0 0.0 53.354716095106035 0.0 34 0.001772052080610649 0.0 0.0 54.194447274805405 0.0 35 0.001772052080610649 0.0 0.0 55.03484297403501 0.0 36 0.001772052080610649 0.0 0.0 55.847771866015144 0.0 37 0.001772052080610649 0.0 0.0 56.60178002631498 0.0 38 0.00199355859068698 0.0 0.0 57.37350870742091 0.0 39 0.0053161562418319475 0.0 0.0 58.18267198872975 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTC 20 7.02873E-4 45.0 6 AACCGAA 20 7.02873E-4 45.0 39 CTCGTTC 20 7.02873E-4 45.0 45 TTTACGG 40 6.7993824E-9 45.0 2 CGCGGGT 20 7.02873E-4 45.0 4 CGTGCGG 25 3.8870297E-5 45.0 2 TAGACGG 25 3.8870297E-5 45.0 2 TACGGGT 40 6.7993824E-9 45.0 4 ACGGGTC 20 7.02873E-4 45.0 5 GTAATCG 25 3.8870297E-5 45.0 1 TAACCGA 20 7.02873E-4 45.0 38 TCGTGCA 20 7.02873E-4 45.0 22 TACTGCG 25 3.8870297E-5 45.0 1 CGCTAAT 20 7.02873E-4 45.0 15 GTGTACG 25 3.8870297E-5 45.0 1 TTGAACG 20 7.02873E-4 45.0 1 GAACGAA 20 7.02873E-4 45.0 9 GCGATAT 25 3.8870297E-5 45.0 9 GCATGCG 20 7.02873E-4 45.0 1 CGATCCA 20 7.02873E-4 45.0 10 >>END_MODULE