FastQCFastQC Report
Sat 18 Jun 2016
SRR3552721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499922
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC82880.5525620665607944TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT66940.44628987374010115TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC55800.3720193450059403TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC40970.2731475370052576TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC24940.16627531298294176TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC22850.15234125507859742No Hit
GCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG16910.11273919577151345No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG453.8562575E-1045.0000041
AGCGTTA207.033976E-445.01
TATTGCG800.042.18751
GTTAGCG700.041.7857131
TGCGACG650.041.538461
TACGTAG650.041.538461
TAGCACG603.6379788E-1241.2500041
GCCGATA451.9290383E-840.0000049
TTACGCG403.4591721E-739.3751
TAATGCG403.4591721E-739.3751
TAGCGAG1450.038.7931021
ATTAACG356.2495637E-638.5714261
GCGTACG950.037.8947371
CGCGAGG2450.037.653062
ACGTGCG601.5643309E-1037.5000041
ACGTCCC301.1400877E-437.50000439
GGCTACG601.5643309E-1037.5000041
ACACGAC2050.037.31707426
CGAGGGA8600.036.8895344
CGTTTTT13800.036.8478281
Produced by FastQC (version 0.11.5)