##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552721_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1499922
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53458713186419	31.0	31.0	34.0	30.0	34.0
2	31.692982035065825	31.0	31.0	34.0	30.0	34.0
3	31.745610105058795	31.0	31.0	34.0	30.0	34.0
4	35.37001257398718	37.0	35.0	37.0	33.0	37.0
5	35.37621889671596	37.0	35.0	37.0	33.0	37.0
6	35.40032748369582	37.0	35.0	37.0	33.0	37.0
7	35.861721476183426	37.0	35.0	37.0	35.0	37.0
8	35.89969678423278	37.0	35.0	37.0	35.0	37.0
9	37.634356986563304	39.0	37.0	39.0	35.0	39.0
10	36.89373580759533	39.0	37.0	39.0	32.0	39.0
11	36.71385245366093	39.0	37.0	39.0	32.0	39.0
12	36.650627832647295	39.0	35.0	39.0	32.0	39.0
13	36.65975364052264	39.0	35.0	39.0	32.0	39.0
14	37.71510585217098	39.0	37.0	41.0	33.0	41.0
15	37.689216505924975	39.0	36.0	41.0	33.0	41.0
16	37.70551335336104	39.0	36.0	41.0	33.0	41.0
17	37.69006921693261	39.0	36.0	41.0	33.0	41.0
18	37.67446707228776	39.0	36.0	41.0	33.0	41.0
19	37.674776421707264	39.0	36.0	41.0	32.0	41.0
20	37.566071435714655	39.0	36.0	41.0	32.0	41.0
21	37.53484981219023	39.0	36.0	41.0	32.0	41.0
22	37.52821146699628	39.0	36.0	41.0	32.0	41.0
23	37.45828783096721	39.0	36.0	41.0	32.0	41.0
24	37.458485841263744	39.0	36.0	41.0	32.0	41.0
25	37.418607767603916	39.0	36.0	41.0	32.0	41.0
26	37.339301643685474	39.0	36.0	41.0	32.0	41.0
27	37.281237957707134	39.0	36.0	41.0	32.0	41.0
28	37.24562877269618	39.0	36.0	41.0	32.0	41.0
29	37.19191397952694	39.0	36.0	41.0	31.0	41.0
30	37.114027262750994	39.0	36.0	41.0	31.0	41.0
31	37.061173181005415	39.0	36.0	40.0	31.0	41.0
32	36.97631276826395	39.0	35.0	40.0	31.0	41.0
33	36.92566213443099	39.0	35.0	40.0	31.0	41.0
34	36.86510431875791	39.0	35.0	40.0	31.0	41.0
35	36.830971877204284	39.0	35.0	40.0	31.0	41.0
36	36.73648229707945	39.0	35.0	40.0	30.0	41.0
37	36.68392623083067	39.0	35.0	40.0	30.0	41.0
38	36.65745085411108	39.0	35.0	40.0	30.0	41.0
39	36.62151831895259	39.0	35.0	40.0	30.0	41.0
40	36.545852384323986	39.0	35.0	40.0	30.0	41.0
41	36.46231070682342	39.0	35.0	40.0	30.0	41.0
42	36.38527203414578	39.0	35.0	40.0	30.0	41.0
43	36.28540950796108	38.0	35.0	40.0	30.0	41.0
44	36.231894725192376	38.0	35.0	40.0	30.0	41.0
45	36.18054605506153	38.0	35.0	40.0	30.0	41.0
46	36.04558570378993	38.0	35.0	40.0	29.0	41.0
47	35.92913964859506	38.0	35.0	40.0	29.0	41.0
48	35.872726048421185	38.0	35.0	40.0	29.0	41.0
49	35.81510838563605	38.0	34.0	40.0	28.0	41.0
50	35.69331471903206	38.0	34.0	40.0	28.0	41.0
51	34.40506039647395	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	53.0
10	79.0
11	53.0
12	40.0
13	23.0
14	31.0
15	38.0
16	87.0
17	162.0
18	326.0
19	610.0
20	1075.0
21	1693.0
22	2625.0
23	4054.0
24	5996.0
25	8530.0
26	11331.0
27	14400.0
28	18403.0
29	23849.0
30	30829.0
31	39626.0
32	51358.0
33	67459.0
34	97518.0
35	116928.0
36	138482.0
37	194457.0
38	307099.0
39	362643.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86522899190758	20.412661458395835	26.974736019606354	9.74737353009023
2	31.018146276939735	23.553158097554405	26.956268392623084	18.472427232882776
3	31.69224799689584	24.309930783067387	27.02087175199777	16.976949468039003
4	28.08879395061877	26.761391592362806	26.421973942645018	18.727840514373415
5	24.766154506701017	30.10963236754978	25.935348638129184	19.188864487620023
6	25.356118518162944	35.357971947874624	24.265995165081918	15.019914368880515
7	81.96859570030975	5.045062343241848	8.02321720729478	4.963124749153622
8	81.9849965531541	3.7423279343859215	8.135489712131697	6.137185800328284
9	76.30436782712701	6.520405727764511	10.856897892023719	6.31832855308476
10	36.59190277894451	29.337992242263265	18.88071513052012	15.18938984827211
11	24.905628426011486	26.34023635895733	27.629236720309457	21.124898494721727
12	21.98407650531161	23.95757912744796	33.06298594193564	20.995358425304783
13	22.580040828789762	24.985499245960792	34.304650508493104	18.129809416756338
14	20.506999697317593	27.59716838608941	30.917407705200674	20.978424211392326
15	19.28726960468611	27.05614025262647	33.66961748677598	19.98697265591144
16	21.4725832409952	27.347955427015535	29.80481651712556	21.374644814863704
17	21.290973797304126	26.78665957296446	28.579552803412444	23.34281382631897
18	21.87213735114226	25.596464349479508	31.896991976916134	20.6344063224621
19	21.06896225270381	27.500496692494675	29.429797016111504	22.000744038690012
20	21.281706648745736	28.271270106045517	29.91708902196247	20.529934223246276
21	21.779732546092397	28.20099978532217	30.421781932660497	19.597485735924934
22	20.29152182580161	25.780540588110583	28.418211080309508	25.509726505778303
23	19.03832332614629	27.3825572263091	29.50640099951864	24.072718448025967
24	20.56440268227281	25.789341045734375	29.891154340025683	23.75510193196713
25	19.153595986991323	28.399143422124617	27.579567470841816	24.86769312004224
26	18.984653868667838	29.034776475043365	29.136248418251082	22.84432123803771
27	20.792214528488813	26.859330018494294	28.162664458551845	24.185790994465044
28	17.333101321268707	26.77492562946607	29.090246026126692	26.80172702313854
29	19.328671757598062	25.754405895773246	28.032657698200307	26.884264648428385
30	20.57006964362147	26.073955845703978	27.31015346131332	26.045821049361233
31	18.613167884730007	27.620502932819175	25.18437625423189	28.581952928218936
32	18.168144743526664	27.776911066042096	26.887531484970552	27.167412705460688
33	18.166944681123418	25.97255057262978	27.625969883767286	28.234534862479517
34	17.233562811932888	25.467390970997155	28.171664926576184	29.12738129049377
35	16.919546483083785	26.30176769191998	28.426878197666277	28.351807627329954
36	17.883929964358146	27.939519521681795	27.83731420700543	26.33923630695463
37	17.716587929238987	25.876812260904238	27.832913978193535	28.573685831663248
38	17.155958776523043	26.471976542780222	28.677557899677446	27.694506781019278
39	19.515614811970224	25.178175931815122	29.130514786768913	26.175694469445745
40	19.85503246168801	23.466286913586174	31.030946942574346	25.647733682151475
41	17.520977757510057	26.460842630483448	28.238735080890876	27.779444531115615
42	19.360673421684595	26.235097558406373	28.06412600121873	26.340103018690304
43	19.72102549332565	24.59687903770996	28.566418787110265	27.115676681854122
44	18.625968550364618	24.192658018216946	27.16621264305744	30.015160788360994
45	19.753027157412184	21.895738578406075	28.272870189249844	30.078364074931898
46	20.56713615774687	23.36428160931035	29.202318520563068	26.866263712379713
47	16.436854716445254	24.479539602726007	31.499304630507453	27.584301050321287
48	17.340301695688176	24.027316087103195	30.164635227698504	28.467746989510122
49	19.96790499772655	22.125483858493975	31.3706979429597	26.535913200819778
50	18.67997135851064	21.105764166403322	30.788134316317784	29.426130158768256
51	18.208746854836452	20.64434017235563	28.279670542868228	32.867242429939694
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	227.0
1	432.5
2	638.0
3	1319.5
4	2001.0
5	1616.5
6	1232.0
7	1353.5
8	1475.0
9	1553.0
10	1631.0
11	1723.5
12	1816.0
13	1985.5
14	2155.0
15	2329.5
16	2504.0
17	2773.5
18	3043.0
19	3088.5
20	3134.0
21	3637.0
22	4140.0
23	4616.0
24	5092.0
25	6356.0
26	9426.0
27	11232.0
28	14253.0
29	17274.0
30	19757.5
31	22241.0
32	25503.0
33	28765.0
34	31935.0
35	35105.0
36	40557.0
37	46009.0
38	50940.5
39	55872.0
40	60874.0
41	65876.0
42	75224.5
43	84573.0
44	97664.5
45	110756.0
46	133695.0
47	156634.0
48	182098.0
49	207562.0
50	200169.0
51	192776.0
52	161523.0
53	130270.0
54	108046.0
55	85822.0
56	72609.5
57	59397.0
58	52017.5
59	44638.0
60	38456.5
61	32275.0
62	28351.0
63	24427.0
64	21216.0
65	18005.0
66	15108.5
67	12212.0
68	9619.5
69	7027.0
70	6182.0
71	5337.0
72	4462.0
73	3587.0
74	2989.5
75	2004.0
76	1616.0
77	1138.5
78	661.0
79	547.5
80	434.0
81	322.5
82	211.0
83	168.0
84	125.0
85	86.5
86	48.0
87	42.0
88	36.0
89	21.5
90	7.0
91	6.0
92	5.0
93	3.5
94	2.0
95	1.0
96	0.0
97	2.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1499922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.71842260340321
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.46424726184854	21.746053299302453
2	14.065079849691628	9.203744529456431
3	5.719965088737392	5.614447051500682
4	2.994781449354054	3.9193810025919325
5	1.7402806155057335	2.8469618313313623
6	1.130973683903879	2.2202204965976926
7	0.8620106002338873	1.974253897494591
8	0.6489991301992842	1.6987382248881029
9	0.5415424016652597	1.5946571839811303
>10	5.585557003181813	38.18535361875494
>50	0.17802051937654975	3.705471026522921
>100	0.05974442658894301	3.4085487121072733
>500	0.006547326298039951	1.4505249105168747
>1k	0.0016368315745099878	1.055158465920573
>5k	6.138118404412454E-4	1.376485749033043
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	8288	0.5525620665607944	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT	6694	0.44628987374010115	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	5580	0.3720193450059403	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	4097	0.2731475370052576	TruSeq Adapter, Index 16 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC	2494	0.16627531298294176	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCC	2285	0.15234125507859742	No Hit
GCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG	1691	0.11273919577151345	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3334026702721874E-4	0.0	0.0	0.49155889439584194	0.0
2	1.3334026702721874E-4	0.0	0.0	1.9920369192531344	0.0
3	1.3334026702721874E-4	0.0	0.0	2.7622769717358637	0.0
4	1.3334026702721874E-4	0.0	0.0	3.7175266447188586	0.0
5	1.3334026702721874E-4	0.0	0.0	6.019779695210818	0.0
6	1.3334026702721874E-4	0.0	0.0	7.266911212716394	0.0
7	1.3334026702721874E-4	0.0	0.0	8.60878098994481	0.0
8	1.3334026702721874E-4	0.0	0.0	10.8910996705162	0.0
9	1.3334026702721874E-4	0.0	0.0	11.612003824198858	0.0
10	1.3334026702721874E-4	0.0	0.0	13.500768706639413	0.0
11	1.3334026702721874E-4	0.0	0.0	16.285913534170444	0.0
12	1.3334026702721874E-4	0.0	0.0	18.29221786199549	0.0
13	1.3334026702721874E-4	0.0	0.0	19.09552630070097	0.0
14	1.3334026702721874E-4	0.0	0.0	19.393341787106262	0.0
15	1.3334026702721874E-4	0.0	0.0	19.997373196739563	0.0
16	1.3334026702721874E-4	0.0	0.0	21.306107917611715	0.0
17	1.3334026702721874E-4	0.0	0.0	23.074133188259122	0.0
18	1.3334026702721874E-4	0.0	0.0	24.889027562766596	0.0
19	1.3334026702721874E-4	0.0	0.0	26.02395324556877	0.0
20	1.3334026702721874E-4	0.0	0.0	27.053673457686465	0.0
21	4.0002080108165626E-4	0.0	0.0	28.332073267809925	0.0
22	4.666909345952656E-4	0.0	0.0	29.69881100483892	0.0
23	5.33361068108875E-4	0.0	0.0	31.06348196772899	0.0
24	7.333714686497032E-4	0.0	0.0	32.09793575932615	0.0
25	0.00106672213621775	0.0	0.0	33.02345055276208	0.0
26	0.0011333922697313594	0.0	0.0	33.865561009172474	0.0
27	0.0011333922697313594	0.0	0.0	34.68593700205744	0.0
28	0.0011333922697313594	0.0	0.0	35.53978140196624	0.0
29	0.0012667325367585781	0.0	0.0	36.40842657151505	0.0
30	0.0012667325367585781	0.0	0.0	37.36287620289588	0.0
31	0.0012667325367585781	0.0	0.0	38.224921029226856	0.0
32	0.0012667325367585781	0.0	0.0	39.034896481283695	0.0
33	0.0012667325367585781	0.0	0.0	39.85507246376812	0.0
34	0.0014667429372994063	0.0	0.0	40.62244570050976	0.0
35	0.0015334130708130156	0.0	0.0	41.408553244768726	0.0
36	0.0015334130708130156	0.0	0.0	42.13972459901248	0.0
37	0.0015334130708130156	0.0	0.0	42.884896681294094	0.0
38	0.001600083204326625	0.0	0.0	43.64726965802222	0.0
39	0.001600083204326625	0.0	0.0	44.40804255154601	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGCG	45	3.8562575E-10	45.000004	1
AGCGTTA	20	7.033976E-4	45.0	1
TATTGCG	80	0.0	42.1875	1
GTTAGCG	70	0.0	41.785713	1
TGCGACG	65	0.0	41.53846	1
TACGTAG	65	0.0	41.53846	1
TAGCACG	60	3.6379788E-12	41.250004	1
GCCGATA	45	1.9290383E-8	40.000004	9
TTACGCG	40	3.4591721E-7	39.375	1
TAATGCG	40	3.4591721E-7	39.375	1
TAGCGAG	145	0.0	38.793102	1
ATTAACG	35	6.2495637E-6	38.571426	1
GCGTACG	95	0.0	37.894737	1
CGCGAGG	245	0.0	37.65306	2
ACGTGCG	60	1.5643309E-10	37.500004	1
ACGTCCC	30	1.1400877E-4	37.500004	39
GGCTACG	60	1.5643309E-10	37.500004	1
ACACGAC	205	0.0	37.317074	26
CGAGGGA	860	0.0	36.889534	4
CGTTTTT	1380	0.0	36.847828	1
>>END_MODULE