FastQCFastQC Report
Sat 18 Jun 2016
SRR3552719_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552719_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences915600
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC39630.43283093053735255Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT36490.3985364788117082Illumina PCR Primer Index 6 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25230.27555701179554387No Hit
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC21380.23350808213193536Illumina PCR Primer Index 6 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC17830.19473569244211447Illumina PCR Primer Index 6 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC14450.15782000873743993No Hit
ACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC12140.1325906509392748Illumina PCR Primer Index 6 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG9740.10637833114897335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCAT351.2115743E-745.010
CAGACGA207.0325343E-445.024
CGTTGAT600.044.99999625
GCCGATA302.1650158E-644.9999969
CCCTCGT950.040.26315714
CGTTAGG950.040.2631572
CGACCAA1800.040.029
GCGAGAC2050.039.51219621
CCGATAT403.4571895E-739.37511
GTGTACG403.4571895E-739.3751
CGTTTGG800.039.3752
GGCCTAT800.039.3758
GTACGAG403.4571895E-739.3751
CACGACC1900.039.07894527
CGTTTTT18000.038.751
CCTTCGA356.246897E-638.57142620
ATTTACG356.246897E-638.5714261
TATCGAG356.246897E-638.5714261
CTTACGA356.246897E-638.57142616
CCTCGTG1000.038.2515