##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552719_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	915600
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49364132809087	31.0	31.0	34.0	30.0	34.0
2	31.64521734381826	31.0	31.0	34.0	30.0	34.0
3	31.666475535168196	31.0	31.0	34.0	30.0	34.0
4	35.33491917868065	37.0	35.0	37.0	33.0	37.0
5	35.345065530799474	37.0	35.0	37.0	33.0	37.0
6	35.365618173875056	37.0	35.0	37.0	33.0	37.0
7	35.812460681520314	37.0	35.0	37.0	35.0	37.0
8	35.85831039755352	37.0	35.0	37.0	35.0	37.0
9	37.591890563564874	39.0	37.0	39.0	35.0	39.0
10	36.952710790738315	39.0	37.0	39.0	33.0	39.0
11	36.73839012669288	39.0	35.0	39.0	32.0	39.0
12	36.47470948012232	38.0	35.0	39.0	32.0	39.0
13	36.36767911751857	38.0	35.0	39.0	32.0	39.0
14	37.11042267365662	39.0	36.0	41.0	31.0	41.0
15	37.172757754477935	39.0	36.0	41.0	31.0	41.0
16	37.27159021406728	39.0	36.0	41.0	32.0	41.0
17	37.29913171690695	39.0	36.0	41.0	32.0	41.0
18	37.29949213630406	39.0	36.0	41.0	32.0	41.0
19	37.30263652249891	39.0	35.0	41.0	32.0	41.0
20	37.16576998689384	39.0	35.0	40.0	32.0	41.0
21	37.09400502402796	39.0	35.0	40.0	31.0	41.0
22	37.14475972040192	39.0	35.0	40.0	32.0	41.0
23	37.129029051987764	39.0	35.0	40.0	32.0	41.0
24	37.10999890782001	39.0	35.0	40.0	32.0	41.0
25	37.01635102664919	39.0	35.0	40.0	31.0	41.0
26	36.969699650502406	39.0	35.0	40.0	31.0	41.0
27	36.89160878112713	39.0	35.0	40.0	31.0	41.0
28	36.799059633027525	39.0	35.0	40.0	31.0	41.0
29	36.6959130624727	39.0	35.0	40.0	31.0	41.0
30	36.54421363040629	38.0	35.0	40.0	30.0	41.0
31	36.346535605067714	38.0	35.0	40.0	30.0	41.0
32	36.245363695937094	38.0	35.0	40.0	30.0	41.0
33	36.140123416339016	38.0	35.0	40.0	30.0	41.0
34	35.918099606815204	38.0	34.0	40.0	29.0	41.0
35	35.843256880733946	38.0	34.0	40.0	29.0	41.0
36	35.75707951070336	38.0	34.0	40.0	29.0	41.0
37	35.57156618610747	38.0	34.0	40.0	28.0	41.0
38	35.49052752293578	38.0	34.0	40.0	27.0	41.0
39	35.42032219309742	38.0	34.0	40.0	27.0	41.0
40	35.24754914809961	38.0	34.0	40.0	26.0	41.0
41	35.15788444735693	38.0	34.0	40.0	26.0	41.0
42	35.12327107907383	38.0	34.0	40.0	26.0	41.0
43	35.02329510703364	38.0	34.0	40.0	26.0	41.0
44	34.93267693315858	38.0	34.0	40.0	26.0	41.0
45	34.82945500218436	37.0	33.0	40.0	26.0	41.0
46	34.66955220620358	37.0	33.0	40.0	25.0	41.0
47	34.48899847094801	37.0	33.0	40.0	24.0	41.0
48	34.42633136740935	37.0	33.0	40.0	24.0	41.0
49	34.359985801660116	36.0	33.0	40.0	24.0	41.0
50	34.19751092179991	36.0	33.0	40.0	24.0	41.0
51	32.854787024901704	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	30.0
10	48.0
11	44.0
12	27.0
13	19.0
14	19.0
15	51.0
16	78.0
17	171.0
18	354.0
19	683.0
20	1112.0
21	1828.0
22	2824.0
23	4036.0
24	5955.0
25	8424.0
26	10845.0
27	13435.0
28	16007.0
29	19137.0
30	23557.0
31	29483.0
32	37608.0
33	49404.0
34	72319.0
35	75073.0
36	84121.0
37	115103.0
38	169509.0
39	174246.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.082459589340324	19.682284840541723	25.580602883355176	10.654652686762779
2	33.4346876365225	23.083224115334207	24.805482743556137	18.676605504587158
3	32.745849716033206	23.845456531236348	27.166994320664045	16.241699432066405
4	29.019659239842728	25.882918304936652	25.981869812145042	19.115552643075578
5	25.396898208824815	29.52599388379205	25.35091743119266	19.726190476190474
6	26.505679335954564	33.385211882918306	25.21526867627785	14.893840104849279
7	78.88215377894277	6.1047400611620795	9.657273918741808	5.355832241153342
8	78.48612931411097	4.973787680209699	10.210135430318916	6.32994757536042
9	73.3385757972914	6.546526867627785	12.979139362166887	7.135757972913936
10	40.9829619921363	23.7371122761031	19.45740498034076	15.822520751419834
11	31.120030581039753	24.388706858890345	24.322083879423328	20.16917868064657
12	27.1214504150284	22.13204456094364	30.847640891218873	19.898864132809088
13	26.00546089995631	22.354303189165574	32.61784622105723	19.02238968982088
14	20.178025338575797	25.073831367409348	33.641000436872	21.107142857142858
15	19.60615989515072	23.93730886850153	36.10430318916558	20.352228047182173
16	23.576343381389254	25.59764089121887	30.053298383573612	20.77271734381826
17	23.681738750546092	24.21876365224989	28.596002621231982	23.50349497597204
18	24.450851900393182	23.101135867190912	31.504150283966798	20.943861948449104
19	23.771515945827872	25.57645259938838	28.678352992573174	21.97367846221057
20	23.03058103975535	28.344692005242468	29.050786369593705	19.573940585408476
21	21.698885976408913	27.07055482743556	32.01463521188292	19.21592398427261
22	19.932284840541723	24.676277850589777	30.44375273044998	24.947684578418524
23	20.05515508955876	25.982525120139798	30.988750546089992	22.973569244211443
24	21.151813018785496	25.76605504587156	29.266601135867194	23.815530799475752
25	22.07525120139799	26.28112712975098	26.863368283093052	24.780253385757973
26	21.247815640017475	24.988750546089996	28.81957186544343	24.943861948449104
27	22.59753167321975	23.955439056356486	29.745740498034074	23.701288772389688
28	18.21526867627785	25.923765836609874	28.253713411970296	27.607252075141986
29	23.26518130187855	26.031891655744865	26.913280908693753	23.789646133682833
30	26.139908256880734	24.323831367409348	27.407273918741808	22.128986456968107
31	23.147335080821318	27.75032765399738	25.713084316295326	23.38925294888598
32	24.84534731323722	27.87712975098296	24.314329401485367	22.96319353429445
33	23.722804718217564	24.92540410659677	25.821647007426822	25.530144167758845
34	19.81061598951507	26.19812145041503	29.233508082131937	24.757754477937965
35	22.004259501965922	28.833114897335083	26.091524683267803	23.071100917431192
36	24.025010921799915	27.541612057667102	24.956858890345128	23.476518130187856
37	21.245849716033202	29.71898208824814	25.110528615115772	23.924639580602882
38	20.062691131498468	29.937745740498034	25.535168195718654	24.46439493228484
39	20.710463084316295	25.070664045434686	27.483944954128443	26.734927916120576
40	22.733180428134556	25.37013979903888	29.731979030144167	22.164700742682395
41	20.714176496286587	26.754914809960685	24.924858016601135	27.606050677151593
42	22.332131935342943	24.373853211009173	27.555264307557888	25.738750546089996
43	21.98099606815203	22.667649628658804	29.111730013106158	26.239624290083007
44	21.84010484927916	23.101463521188293	27.30777632153779	27.75065530799476
45	22.41360856269113	22.165465268676275	27.560834425513324	27.860091743119263
46	22.145150720838792	24.209698558322412	28.156727828746174	25.488422892092615
47	18.554390563564876	25.952380952380956	30.625382262996943	24.86784622105723
48	19.208278724333773	25.990170380078638	28.84949759720402	25.952053298383575
49	22.56127129750983	22.800567933595456	30.119156837046745	24.51900393184797
50	19.591743119266056	23.25644386194845	29.24235474006116	27.909458278724337
51	19.293359545653125	22.841961555264305	26.981979030144167	30.882699868938403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	226.0
1	543.0
2	860.0
3	2648.5
4	4437.0
5	3161.0
6	1885.0
7	1834.5
8	1784.0
9	1876.5
10	1969.0
11	2020.0
12	2071.0
13	2234.5
14	2398.0
15	2461.0
16	2524.0
17	2527.0
18	2530.0
19	2478.5
20	2427.0
21	2751.0
22	3075.0
23	3323.5
24	3572.0
25	4455.5
26	6190.0
27	7041.0
28	8235.0
29	9429.0
30	10802.5
31	12176.0
32	12869.5
33	13563.0
34	15804.5
35	18046.0
36	19417.5
37	20789.0
38	23194.5
39	25600.0
40	28631.5
41	31663.0
42	37160.0
43	42657.0
44	49001.5
45	55346.0
46	68099.0
47	80852.0
48	95802.0
49	110752.0
50	111610.0
51	112468.0
52	97931.0
53	83394.0
54	72982.0
55	62570.0
56	55504.0
57	48438.0
58	44213.0
59	39988.0
60	35282.5
61	30577.0
62	27342.0
63	24107.0
64	20365.5
65	16624.0
66	14093.0
67	11562.0
68	9472.0
69	7382.0
70	6325.5
71	5269.0
72	4487.0
73	3705.0
74	3217.5
75	2210.5
76	1691.0
77	1341.0
78	991.0
79	777.5
80	564.0
81	407.0
82	250.0
83	205.5
84	161.0
85	111.0
86	61.0
87	41.5
88	22.0
89	19.0
90	16.0
91	14.0
92	12.0
93	8.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	915600.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.88524122572498
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1098856345763	26.18244688348189
2	12.613668415461355	9.557437413221233
3	5.089762903487878	5.784806861411539
4	2.750347408430792	4.167903000917924
5	1.6995976328017308	3.219483315268236
6	1.2032627937017883	2.7351540718399203
7	0.9457010304447311	2.507967816607073
8	0.7741055889158858	2.3461741576208186
9	0.659305262467979	2.248014501899038
>10	5.022714568106559	33.114816002608094
>50	0.08025981445035602	2.083944041866839
>100	0.04619231204984736	3.3784976662802793
>500	0.0031757214529654675	0.8451439497967873
>1k	0.002020913651887116	1.828210317180445
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	3963	0.43283093053735255	Illumina PCR Primer Index 6 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGCT	3649	0.3985364788117082	Illumina PCR Primer Index 6 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2523	0.27555701179554387	No Hit
CCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	2138	0.23350808213193536	Illumina PCR Primer Index 6 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1783	0.19473569244211447	Illumina PCR Primer Index 6 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCC	1445	0.15782000873743993	No Hit
ACTGTCTCTTATACACATCTGACGCGGCATAACTCGTATGCCGTCTTCTGC	1214	0.1325906509392748	Illumina PCR Primer Index 6 (95% over 23bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	974	0.10637833114897335	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0921799912625601E-4	0.0	0.0	0.49148099606815204	0.0
2	1.0921799912625601E-4	0.0	0.0	1.751310615989515	0.0
3	1.0921799912625601E-4	0.0	0.0	2.4259501965923986	0.0
4	1.0921799912625601E-4	0.0	0.0	3.2202927042376586	0.0
5	1.0921799912625601E-4	0.0	0.0	5.351135867190913	0.0
6	1.0921799912625601E-4	0.0	0.0	6.525666229794671	0.0
7	1.0921799912625601E-4	0.0	0.0	7.946810834425514	0.0
8	1.0921799912625601E-4	0.0	0.0	10.171144604630843	0.0
9	1.0921799912625601E-4	0.0	0.0	10.908912188728703	0.0
10	1.0921799912625601E-4	0.0	0.0	12.76834862385321	0.0
11	1.0921799912625601E-4	0.0	0.0	15.72051114023591	0.0
12	1.0921799912625601E-4	0.0	0.0	17.77861511577108	0.0
13	1.0921799912625601E-4	0.0	0.0	18.642857142857142	0.0
14	1.0921799912625601E-4	0.0	0.0	18.98339886413281	0.0
15	1.0921799912625601E-4	0.0	0.0	19.66655744866754	0.0
16	1.0921799912625601E-4	0.0	0.0	21.007208387942335	0.0
17	1.0921799912625601E-4	0.0	0.0	22.60594145915247	0.0
18	1.0921799912625601E-4	0.0	0.0	24.329838357361293	0.0
19	1.0921799912625601E-4	0.0	0.0	25.395150720838796	0.0
20	1.0921799912625601E-4	0.0	0.0	26.460135430318918	0.0
21	2.1843599825251202E-4	0.0	0.0	27.72728265618174	0.0
22	2.1843599825251202E-4	0.0	0.0	28.984927916120576	0.0
23	6.55307994757536E-4	0.0	0.0	30.187964176496287	0.0
24	9.82961992136304E-4	0.0	0.0	31.176823940585408	0.0
25	0.0017474879860200961	0.0	0.0	32.10474006116208	0.0
26	0.001856705985146352	0.0	0.0	32.903123634775014	0.0
27	0.001856705985146352	0.0	0.0	33.79969418960245	0.0
28	0.001856705985146352	0.0	0.0	34.642201834862384	0.0
29	0.001856705985146352	0.0	0.0	35.524574049803405	0.0
30	0.001856705985146352	0.0	0.0	36.54106596767147	0.0
31	0.001856705985146352	0.0	0.0	37.36664482306684	0.0
32	0.001856705985146352	0.0	0.0	38.19702927042377	0.0
33	0.001856705985146352	0.0	0.0	39.04193971166448	0.0
34	0.001856705985146352	0.0	0.0	39.88608562691132	0.0
35	0.001856705985146352	0.0	0.0	40.7412625600699	0.0
36	0.001856705985146352	0.0	0.0	41.4825251201398	0.0
37	0.002075141983398864	0.0	0.0	42.281127129750985	0.0
38	0.002075141983398864	0.0	0.0	43.15410659676715	0.0
39	0.002075141983398864	0.0	0.0	44.58420707732634	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCAT	35	1.2115743E-7	45.0	10
CAGACGA	20	7.0325343E-4	45.0	24
CGTTGAT	60	0.0	44.999996	25
GCCGATA	30	2.1650158E-6	44.999996	9
CCCTCGT	95	0.0	40.263157	14
CGTTAGG	95	0.0	40.263157	2
CGACCAA	180	0.0	40.0	29
GCGAGAC	205	0.0	39.512196	21
CCGATAT	40	3.4571895E-7	39.375	11
GTGTACG	40	3.4571895E-7	39.375	1
CGTTTGG	80	0.0	39.375	2
GGCCTAT	80	0.0	39.375	8
GTACGAG	40	3.4571895E-7	39.375	1
CACGACC	190	0.0	39.078945	27
CGTTTTT	1800	0.0	38.75	1
CCTTCGA	35	6.246897E-6	38.571426	20
ATTTACG	35	6.246897E-6	38.571426	1
TATCGAG	35	6.246897E-6	38.571426	1
CTTACGA	35	6.246897E-6	38.571426	16
CCTCGTG	100	0.0	38.25	15
>>END_MODULE