FastQCFastQC Report
Sat 18 Jun 2016
SRR3552718_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552718_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316641
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.197700234650598No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC5340.16864524808852926No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3420.10800875439377718No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3350.10579804889448935No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC253.8843827E-545.06
CCGATTG207.0255366E-445.011
CCGCTAA253.8843827E-545.019
ATCAATT253.8843827E-545.011
CCCACCG207.0255366E-445.024
TAGTTAG207.0255366E-445.01
TCGGAGA207.0255366E-445.045
AATTGTC207.0255366E-445.019
TGCGACG253.8843827E-545.01
CCATAGT207.0255366E-445.044
CGAATAT700.045.014
AGCGACG207.0255366E-445.023
CTCGAAA207.0255366E-445.019
TAGGACG253.8843827E-545.01
TAGTGCG253.8843827E-545.01
TCGTTAG302.1605028E-644.9999961
ACGCATT302.1605028E-644.99999617
CGCATGG302.1605028E-644.9999962
TCGTGTA600.044.99999617
GCGATAT302.1605028E-644.9999969
Produced by FastQC (version 0.11.5)