##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552718_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	316641
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.906888242520708	33.0	31.0	34.0	30.0	34.0
2	32.036659813479616	33.0	31.0	34.0	30.0	34.0
3	32.06593902874233	33.0	31.0	34.0	30.0	34.0
4	35.71722234328467	37.0	35.0	37.0	33.0	37.0
5	35.59799583755736	37.0	35.0	37.0	33.0	37.0
6	35.53706879399699	37.0	35.0	37.0	33.0	37.0
7	35.78707432076074	37.0	35.0	37.0	35.0	37.0
8	35.58912459220379	37.0	35.0	37.0	35.0	37.0
9	37.42371644859636	39.0	37.0	39.0	35.0	39.0
10	37.01856992619402	39.0	37.0	39.0	33.0	39.0
11	36.899908729444384	39.0	37.0	39.0	33.0	39.0
12	36.75417270662991	39.0	35.0	39.0	33.0	39.0
13	36.60138137512198	39.0	35.0	39.0	32.0	39.0
14	37.78024639891865	40.0	36.0	41.0	33.0	41.0
15	37.95506898980233	40.0	37.0	41.0	33.0	41.0
16	37.999447323625176	40.0	37.0	41.0	33.0	41.0
17	37.926152961871644	40.0	36.0	41.0	33.0	41.0
18	37.93750651368585	40.0	36.0	41.0	33.0	41.0
19	37.89770434024653	40.0	36.0	41.0	33.0	41.0
20	37.779494759048895	40.0	36.0	41.0	33.0	41.0
21	37.624982235402236	40.0	35.0	41.0	33.0	41.0
22	37.65134331940589	40.0	35.0	41.0	33.0	41.0
23	33.60774504880922	39.0	35.0	41.0	0.0	41.0
24	35.241911186485645	39.0	35.0	41.0	17.0	41.0
25	36.329186681446814	39.0	35.0	41.0	29.0	41.0
26	36.894053518021984	39.0	35.0	41.0	31.0	41.0
27	30.789199756190765	38.0	32.0	41.0	0.0	41.0
28	33.298568410281675	38.0	31.0	40.0	16.0	41.0
29	35.2643940614134	38.0	32.0	40.0	25.0	41.0
30	36.24666420330911	38.0	35.0	40.0	30.0	41.0
31	36.641000375819935	38.0	35.0	40.0	31.0	41.0
32	35.14831623194722	38.0	35.0	40.0	26.0	41.0
33	35.78907343016223	39.0	35.0	40.0	27.0	41.0
34	36.231372437555464	39.0	35.0	41.0	30.0	41.0
35	36.37825802722958	39.0	35.0	41.0	30.0	41.0
36	36.40747407947802	39.0	35.0	40.0	30.0	41.0
37	36.431674988393794	39.0	35.0	41.0	30.0	41.0
38	36.35248751740931	39.0	35.0	41.0	30.0	41.0
39	36.27360954519472	39.0	35.0	41.0	30.0	41.0
40	36.20521347519746	38.0	35.0	40.0	30.0	41.0
41	36.12328157124314	38.0	35.0	40.0	30.0	41.0
42	36.060443846501244	38.0	35.0	40.0	30.0	41.0
43	36.02936764348268	38.0	35.0	40.0	30.0	41.0
44	35.92967114176623	38.0	35.0	40.0	30.0	41.0
45	35.8519743179184	38.0	35.0	40.0	30.0	41.0
46	35.723510221354786	38.0	35.0	40.0	29.0	41.0
47	35.64454066276951	38.0	34.0	40.0	29.0	41.0
48	35.588202412195514	37.0	34.0	40.0	29.0	41.0
49	35.55175103666297	37.0	34.0	40.0	29.0	41.0
50	35.40820992859421	37.0	34.0	40.0	29.0	41.0
51	34.71090288370742	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	24.0
10	38.0
11	29.0
12	20.0
13	21.0
14	25.0
15	40.0
16	61.0
17	73.0
18	142.0
19	263.0
20	446.0
21	703.0
22	1069.0
23	1433.0
24	1922.0
25	2384.0
26	3138.0
27	3752.0
28	4605.0
29	5789.0
30	7667.0
31	9586.0
32	12446.0
33	17207.0
34	26034.0
35	30373.0
36	28810.0
37	36733.0
38	50752.0
39	71013.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.679937215963825	18.693409886906622	22.77531968380595	13.851333213323604
2	30.314772881591455	26.25970736575491	25.033397443792815	18.392122308860824
3	32.37230807128578	24.180380936139034	26.568890320583876	16.87842067199131
4	29.50818118942272	25.44648355708831	26.271708338465327	18.773626915023637
5	25.086770190847048	30.67259135740476	24.458298198906647	19.782340252841546
6	25.639762380740333	36.67023537697266	23.831405282322883	13.858596959964123
7	81.78599739136752	6.667487785852116	7.578930081701358	3.9675847410790137
8	83.835005574136	5.8242615454094695	6.514949106401256	3.8257837740532654
9	78.48667734121608	7.723257569297721	7.858742234896934	5.931322854589267
10	43.14033874324551	25.958419787709108	15.281975486434163	15.619265982611221
11	32.754760122662574	26.975344317381516	21.735656468998013	18.5342390909579
12	28.543681961590572	24.25270258747288	27.2741053748567	19.92951007607985
13	24.36450112272258	26.28749909203167	28.596107263430824	20.751892521814927
14	21.326360136558435	29.404278030956192	27.63981922745317	21.6295426050322
15	21.275829725146146	26.127380850868963	30.85955387963024	21.737235544354647
16	23.81845686439848	24.009524982551216	29.998641995193292	22.173376157857007
17	23.42052987452667	25.34100132326515	27.185361339813856	24.053107462394323
18	24.427032506845293	25.55354486626811	27.782883454764228	22.23653917212237
19	26.166226104642163	24.95886508695968	25.948629520497978	22.92627928790018
20	27.01197886565543	23.74392450756535	26.490568182894826	22.7535284438844
21	25.678607634513533	26.195912721346886	26.215177440697822	21.910302203441752
22	25.268995487002634	24.589677268578612	26.021582801974475	24.119744442444283
23	21.79566133255011	34.58301357057362	23.20640725616708	20.414917840709194
24	22.824271019861612	26.49625285417871	28.86423425898731	21.815241866972375
25	23.454953717301297	27.254840655505763	26.849649918993435	22.440555708199504
26	22.78826810173035	27.459488821725554	26.021898617045803	23.730344459498294
27	19.68317432044492	40.11546199007709	21.91346035415502	18.28790333532297
28	22.08115815702957	27.774988077981057	28.81307221743236	21.330781547557013
29	23.184931831316856	26.48235699104033	27.330636272624204	23.002074905018617
30	22.866590239419406	28.151755458073968	26.757747733237323	22.2239065692693
31	25.945155554713384	27.422222643308984	23.818141049327156	22.81448075265048
32	24.39924078056853	30.97482638066454	23.747714288421275	20.878218550345657
33	24.15069431943431	28.320400706162502	23.796349809405605	23.732555164997585
34	23.420214059455347	27.178413408244666	25.16414488332212	24.237227648977864
35	23.575595074548147	27.04956085914332	26.200965762488117	23.173878303820413
36	24.6954753174731	29.63798118373805	23.903095303514075	21.763448195274776
37	23.841195549534014	28.619477578709013	24.339867547159084	23.19945932459789
38	23.734450055425548	29.200577309950386	24.827801832359047	22.237170802265023
39	24.018683619619697	27.324004156126342	25.593653380326614	23.06365884392735
40	24.29565343717333	26.78648690472807	26.58404944400757	22.333810214091038
41	22.03315426618789	26.98387132430734	26.333608092445388	24.649366317059382
42	22.326862282521848	27.320846005413067	26.116011508301202	24.236280203763886
43	23.19409046838533	25.871254828022906	26.626368663565366	24.308286040026402
44	23.33810214091037	26.71227036296626	26.475409059471133	23.474218436652233
45	24.435559513771114	27.178729223315994	24.90201837412085	23.48369288879204
46	24.25365003268686	28.480203132253877	24.97181350488408	22.294333330175185
47	23.571805293692226	27.551706822552983	26.750799801668133	22.125688082086654
48	22.91680483576037	28.125226992082514	26.02316187733111	22.934806294826
49	22.954386829248268	27.65182020016359	27.019242612295947	22.374550358292197
50	22.278858391680167	27.263683477502916	27.580761809115057	22.876696321701864
51	21.793766442122152	27.251682504792495	26.30139495517005	24.653156097915303
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	662.0
1	872.0
2	1082.0
3	1314.0
4	1546.0
5	1150.0
6	754.0
7	768.5
8	783.0
9	823.5
10	864.0
11	784.0
12	704.0
13	728.5
14	753.0
15	748.0
16	743.0
17	808.0
18	873.0
19	863.0
20	853.0
21	973.0
22	1093.0
23	1250.0
24	1407.0
25	1838.5
26	2700.0
27	3130.0
28	3475.0
29	3820.0
30	4552.0
31	5284.0
32	6220.0
33	7156.0
34	8153.5
35	9151.0
36	9449.0
37	9747.0
38	10294.0
39	10841.0
40	12200.0
41	13559.0
42	15061.0
43	16563.0
44	17975.5
45	19388.0
46	21621.0
47	23854.0
48	23514.0
49	23174.0
50	22306.0
51	21438.0
52	21623.0
53	21808.0
54	20413.5
55	19019.0
56	17990.5
57	16962.0
58	16559.5
59	16157.0
60	15289.0
61	14421.0
62	13414.5
63	12408.0
64	11046.5
65	9685.0
66	8416.5
67	7148.0
68	6365.5
69	5583.0
70	4845.0
71	4107.0
72	3649.0
73	3191.0
74	2725.5
75	1677.5
76	1095.0
77	884.0
78	673.0
79	544.5
80	416.0
81	281.5
82	147.0
83	92.0
84	37.0
85	31.0
86	25.0
87	15.0
88	5.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	316641.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.666088725086137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.42705062010965	21.189612210673918
2	9.427795816255921	5.593716543340881
3	3.9463458774684623	3.512179408225719
4	2.1546814286474687	2.556838817462047
5	1.41587267791558	2.100170224323445
6	0.9964337041571298	1.773617440571499
7	0.7899079150476394	1.640343480471575
8	0.6940969819556075	1.6472914120407653
9	0.5514451482408048	1.4723298625256995
>10	8.361100761164634	52.88670765946293
>50	0.19481556395379784	3.46322807217006
>100	0.03832437323681269	1.7976193859923384
>500	0.0021291318464895935	0.36634548273912726
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	626	0.197700234650598	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	534	0.16864524808852926	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	342	0.10800875439377718	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	335	0.10579804889448935	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.049582966198312914	0.0
2	0.0	0.0	0.0	0.19706860450794433	0.0
3	0.0	0.0	0.0	0.2498097214195256	0.0
4	0.0	0.0	0.0	0.3170783316121412	0.0
5	0.0	0.0	0.0	0.5463600733954226	0.0
6	0.0	0.0	0.0	0.7405863422614254	0.0
7	0.0	0.0	0.0	0.9174427822044524	0.0
8	0.0	0.0	0.0	1.2383108946725156	0.0
9	0.0	0.0	0.0	1.3674792588451905	0.0
10	0.0	0.0	0.0	1.7568792417911767	0.0
11	0.0	0.0	0.0	2.1737551359425975	0.0
12	0.0	0.0	0.0	2.558102077747354	0.0
13	0.0	0.0	0.0	2.6717955034250145	0.0
14	0.0	0.0	0.0	2.72011520933802	0.0
15	0.0	0.0	0.0	2.8069643539528992	0.0
16	0.0	0.0	0.0	2.9828733486819456	0.0
17	0.0	0.0	0.0	3.176467987405295	0.0
18	0.0	0.0	0.0	3.432594010251357	0.0
19	0.0	0.0	0.0	3.5611307442813787	0.0
20	0.0	0.0	0.0	3.6773506905296536	0.0
21	0.0	0.0	0.0	3.807150684844982	0.0
22	0.0	0.0	0.0	3.9378981243742914	0.0
23	0.0	0.0	0.0	4.076225125615444	0.0
24	0.0	0.0	0.0	4.206340935002101	0.0
25	0.0	0.0	0.0	4.303611976970765	0.0
26	0.0	0.0	0.0	4.416673772505772	0.0
27	0.0	0.0	0.0	4.521208561114953	0.0
28	0.0	0.0	0.0	4.653535076000897	0.0
29	0.0	0.0	0.0	4.792177892313377	0.0
30	0.0	0.0	0.0	4.9642971061865016	0.0
31	0.0	0.0	0.0	5.113361819852767	0.0
32	0.0	0.0	0.0	5.26463723901832	0.0
33	0.0	0.0	0.0	5.4203340691824495	0.0
34	0.0	0.0	0.0	5.582347200773115	0.0
35	0.0	0.0	0.0	5.774678579211157	0.0
36	0.0	0.0	0.0	5.949640128726223	0.0
37	0.0	0.0	0.0	6.118285376814752	0.0
38	0.0	0.0	0.0	6.30208974832697	0.0
39	0.0	0.0	0.0	6.463787064846309	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	25	3.8843827E-5	45.0	6
CCGATTG	20	7.0255366E-4	45.0	11
CCGCTAA	25	3.8843827E-5	45.0	19
ATCAATT	25	3.8843827E-5	45.0	11
CCCACCG	20	7.0255366E-4	45.0	24
TAGTTAG	20	7.0255366E-4	45.0	1
TCGGAGA	20	7.0255366E-4	45.0	45
AATTGTC	20	7.0255366E-4	45.0	19
TGCGACG	25	3.8843827E-5	45.0	1
CCATAGT	20	7.0255366E-4	45.0	44
CGAATAT	70	0.0	45.0	14
AGCGACG	20	7.0255366E-4	45.0	23
CTCGAAA	20	7.0255366E-4	45.0	19
TAGGACG	25	3.8843827E-5	45.0	1
TAGTGCG	25	3.8843827E-5	45.0	1
TCGTTAG	30	2.1605028E-6	44.999996	1
ACGCATT	30	2.1605028E-6	44.999996	17
CGCATGG	30	2.1605028E-6	44.999996	2
TCGTGTA	60	0.0	44.999996	17
GCGATAT	30	2.1605028E-6	44.999996	9
>>END_MODULE