##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552707_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	975118
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.807970932748653	31.0	31.0	34.0	30.0	34.0
2	31.92562951355631	33.0	31.0	34.0	30.0	34.0
3	31.96187743432077	33.0	31.0	34.0	30.0	34.0
4	35.627920928544036	37.0	35.0	37.0	33.0	37.0
5	35.51660106776821	37.0	35.0	37.0	33.0	37.0
6	35.450581365537296	37.0	35.0	37.0	33.0	37.0
7	35.791541126304715	37.0	35.0	37.0	35.0	37.0
8	35.585897296532316	37.0	35.0	37.0	35.0	37.0
9	37.416273722769965	39.0	37.0	39.0	35.0	39.0
10	36.901672412979764	39.0	37.0	39.0	33.0	39.0
11	36.81968336139831	39.0	37.0	39.0	33.0	39.0
12	36.73089410717472	39.0	35.0	39.0	33.0	39.0
13	36.59897879025923	39.0	35.0	39.0	32.0	39.0
14	37.70531874091136	40.0	36.0	41.0	33.0	41.0
15	37.87177551845007	40.0	37.0	41.0	33.0	41.0
16	37.93529193389928	40.0	37.0	41.0	33.0	41.0
17	37.87528278628843	40.0	36.0	41.0	33.0	41.0
18	37.86974499496471	40.0	36.0	41.0	33.0	41.0
19	37.82972317196483	40.0	36.0	41.0	33.0	41.0
20	37.740902126716975	40.0	36.0	41.0	33.0	41.0
21	37.60374641838219	39.0	36.0	41.0	33.0	41.0
22	37.67780822423543	39.0	36.0	41.0	33.0	41.0
23	33.655814988544975	39.0	35.0	41.0	0.0	41.0
24	35.293940835878324	39.0	35.0	41.0	18.0	41.0
25	36.336934606888605	39.0	34.0	41.0	29.0	41.0
26	36.93550626693385	39.0	35.0	41.0	31.0	41.0
27	30.826170781382356	38.0	32.0	40.0	0.0	41.0
28	33.381747644900415	38.0	31.0	40.0	16.0	41.0
29	35.31105876417008	38.0	32.0	40.0	25.0	41.0
30	36.300049840121915	38.0	35.0	40.0	30.0	41.0
31	36.72479740913408	39.0	35.0	40.0	31.0	41.0
32	35.223203755853135	39.0	35.0	40.0	27.0	41.0
33	35.903137876646724	39.0	35.0	40.0	27.0	41.0
34	36.3553252016679	39.0	35.0	41.0	30.0	41.0
35	36.543418335011765	39.0	35.0	41.0	30.0	41.0
36	36.59663753514959	39.0	35.0	41.0	31.0	41.0
37	36.651414495476445	39.0	35.0	41.0	31.0	41.0
38	36.57294296690247	39.0	35.0	40.0	31.0	41.0
39	36.53369951123864	39.0	35.0	41.0	31.0	41.0
40	36.45322104606827	39.0	35.0	40.0	30.0	41.0
41	36.37682926579142	39.0	35.0	40.0	30.0	41.0
42	36.327727516054466	38.0	35.0	40.0	30.0	41.0
43	36.289779288250244	38.0	35.0	40.0	30.0	41.0
44	36.20889369286589	38.0	35.0	40.0	30.0	41.0
45	36.09887521305114	38.0	35.0	40.0	30.0	41.0
46	36.00126753890299	38.0	35.0	40.0	30.0	41.0
47	35.92193970370765	38.0	35.0	40.0	30.0	41.0
48	35.865368088785154	38.0	35.0	40.0	30.0	41.0
49	35.828100804210365	38.0	35.0	40.0	30.0	41.0
50	35.73025623565558	37.0	34.0	40.0	29.0	41.0
51	34.98122586189569	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	23.0
9	66.0
10	89.0
11	96.0
12	57.0
13	61.0
14	61.0
15	87.0
16	149.0
17	213.0
18	374.0
19	655.0
20	1043.0
21	1659.0
22	2560.0
23	3585.0
24	4896.0
25	6592.0
26	8638.0
27	11229.0
28	14112.0
29	17981.0
30	23566.0
31	30039.0
32	39097.0
33	54002.0
34	79172.0
35	90077.0
36	90906.0
37	116203.0
38	163891.0
39	213824.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.90697536093068	18.463816686800982	22.967886963423915	13.661320988844427
2	31.35138516569277	24.53221046068271	25.804261638078675	18.31214273554585
3	33.17639506193097	24.431094493179287	25.581929571600565	16.810580873289183
4	29.4689463223938	25.63833300174953	24.81012554378034	20.08259513207632
5	25.3578541263724	29.093299477601686	24.440221593694304	21.108624802331615
6	25.378672119681926	35.847046203638946	23.68605645675703	15.088225219922101
7	81.577203989671	6.166945949105647	7.702247317760517	4.553602743462842
8	82.69450466507642	5.683004518427513	6.9996656814867535	4.622825135009301
9	77.29054329834953	7.368031356205096	9.257238611121936	6.084186734323436
10	39.355442110595845	26.76752967333184	17.06634479109195	16.81068342498036
11	30.792068241997377	26.360604562729844	23.045313490264768	19.802013705008008
12	26.287997965374448	24.05811399235785	29.533348784454805	20.1205392578129
13	24.497342885681526	24.78571824127952	30.193166365506535	20.523772507532424
14	20.90546990210416	28.41010011096093	28.43830182603541	22.2461281608995
15	20.688983282023305	26.422340680820167	31.199506111055275	21.68916992610125
16	23.957613335001508	24.53826101046232	29.939043274762646	21.565082379773525
17	23.715693895508032	24.235015659643246	28.032812439109932	24.016478005738794
18	25.375800672328886	23.574787871826793	28.618792802512104	22.430618653332214
19	25.113268342908242	25.7903146080782	26.822907586569013	22.273509462444547
20	25.925067530288644	25.159621707321577	26.825881585613224	22.089429176776555
21	25.474557950935168	26.838597995319542	26.96124981797075	20.72559423577454
22	23.943153546545137	24.24270703648174	26.967710574515085	24.84642884245804
23	19.627983485075653	35.030632190155444	24.469756480754125	20.871627844014775
24	22.626902590250616	25.427999483139473	28.554903098907005	23.390194827702903
25	23.251647492918806	26.40213799765772	27.276801371731423	23.069413137692056
26	21.81274471397308	26.244003289858252	27.59696775159519	24.346284244573475
27	18.28445377892727	38.80750842462143	23.51756402814839	19.39047376830291
28	21.308805703514857	27.150765343271278	29.197697099222864	22.342731853991005
29	22.242538851708204	25.651459618220564	28.66001858236644	23.445982947704792
30	22.66648754304607	25.985573028084808	28.61694687207087	22.730992556798256
31	24.32731218170519	26.421007508834826	26.271589694785657	22.980090614674324
32	22.920405530407603	29.794650493581287	25.79605750278428	21.488886473226827
33	23.368453869172757	25.45476547453744	26.545812917000816	24.63096773928899
34	22.476254155907284	25.64140955248493	28.615716251776707	23.266620039831075
35	22.69007443201746	25.141675161365086	27.964718116166452	24.203532290451
36	23.48351686667665	26.330146710449405	27.086670536283812	23.099665886590135
37	22.660334441575277	27.336896662762868	27.168711889227765	22.834057006434094
38	23.09289747497226	28.671606923469774	26.122069329045306	22.11342627251266
39	22.16131791229369	25.655459134176585	27.75325652895342	24.429966424576307
40	24.405354018693124	24.455194140606572	29.52617016607221	21.613281674628094
41	21.097446667993005	25.006512032389928	28.35841405860624	25.53762724101083
42	22.19577528053015	26.07417768926427	27.09405425804877	24.635992772156808
43	22.241615886487583	25.363802124460832	27.38396788901446	25.010614100037127
44	22.425593620464397	25.465533402111333	28.2615027104412	23.847370266983074
45	23.0231623249699	26.101969197574036	27.527745360048733	23.347123117407328
46	22.797035845918135	26.11099374639787	27.66834372865643	23.423626679027564
47	22.136705506410507	25.832258249770796	29.606980898722	22.424055345096697
48	21.832639742061986	26.45197811957117	28.28796104676562	23.42742109160122
49	21.900221306549568	25.84620527977127	28.916705465389832	23.336867948289335
50	21.552263418376032	25.815952530873187	28.910860018992572	23.72092403175821
51	21.603129057201283	25.10578206945211	27.927902059032856	25.363186814313753
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2496.0
1	2799.5
2	3103.0
3	3437.0
4	3771.0
5	2762.0
6	1753.0
7	1816.0
8	1879.0
9	1882.0
10	1885.0
11	1839.5
12	1794.0
13	1895.5
14	1997.0
15	2158.5
16	2320.0
17	2281.0
18	2242.0
19	2380.0
20	2518.0
21	2780.0
22	3042.0
23	3430.5
24	3819.0
25	5035.0
26	7880.0
27	9509.0
28	10869.0
29	12229.0
30	14088.0
31	15947.0
32	17937.5
33	19928.0
34	23138.0
35	26348.0
36	27936.0
37	29524.0
38	32357.0
39	35190.0
40	38731.0
41	42272.0
42	47071.5
43	51871.0
44	56510.5
45	61150.0
46	66774.0
47	72398.0
48	77156.5
49	81915.0
50	82660.0
51	83405.0
52	78492.5
53	73580.0
54	68217.5
55	62855.0
56	58960.0
57	55065.0
58	50889.0
59	46713.0
60	43961.0
61	41209.0
62	37245.0
63	33281.0
64	30176.0
65	27071.0
66	23114.5
67	19158.0
68	15779.5
69	12401.0
70	10855.5
71	9310.0
72	7561.0
73	5812.0
74	4776.0
75	2997.5
76	2255.0
77	1692.0
78	1129.0
79	800.0
80	471.0
81	410.0
82	349.0
83	220.5
84	92.0
85	66.5
86	41.0
87	29.0
88	17.0
89	13.0
90	9.0
91	6.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	975118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.97401887921776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70891505505317	20.33960562437498
2	10.005033608254234	5.597619980890242
3	4.132726080439357	3.4682687219053854
4	2.2848629897505655	2.55667201646833
5	1.4669016858185941	2.0517567726522854
6	1.0115245676124678	1.6977844410710252
7	0.6816561331336722	1.3348063076191146
8	0.5405013246692926	1.20959954084328
9	0.4225148501773075	1.0637494556048879
>10	5.7641192517916355	40.387510106716086
>50	0.9191641895998371	16.265529574648088
>100	0.05643660336346946	2.7068342586491245
>500	0.004138684246654406	0.8064298969503666
>1k	0.0015049760896925113	0.5138333016068507
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1320	0.1353682323575198	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1257	0.12890747581318363	No Hit
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	1237	0.12685644198958484	TruSeq Adapter, Index 27 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1068	0.10952520618017511	No Hit
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	980	0.10050065735634046	TruSeq Adapter, Index 27 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10091086412106022	0.0
2	0.0	0.0	0.0	0.4700969523688415	0.0
3	0.0	0.0	0.0	0.6209504900945322	0.0
4	0.0	0.0	0.0	0.8101583603215201	0.0
5	0.0	0.0	0.0	1.3947030000471738	0.0
6	0.0	0.0	0.0	1.8598774712393782	0.0
7	0.0	0.0	0.0	2.2609571354441207	0.0
8	0.0	0.0	0.0	2.906827686495378	0.0
9	0.0	0.0	0.0	3.171616153121981	0.0
10	0.0	0.0	0.0	3.841996558365244	0.0
11	0.0	0.0	0.0	4.64230995633349	0.0
12	0.0	0.0	0.0	5.349301315327991	0.0
13	0.0	0.0	0.0	5.593374340336246	0.0
14	0.0	0.0	0.0	5.703617408354681	0.0
15	0.0	0.0	0.0	5.891594658287509	0.0
16	0.0	0.0	0.0	6.2821114983007185	0.0
17	0.0	0.0	0.0	6.711290325888764	0.0
18	0.0	0.0	0.0	7.246815257230407	0.0
19	0.0	0.0	0.0	7.515398136430668	0.0
20	0.0	0.0	0.0	7.761624746953702	0.0
21	0.0	0.0	0.0	8.061280788581485	0.0
22	0.0	0.0	0.0	8.37211496454788	0.0
23	0.0	0.0	0.0	8.686025691249675	0.0
24	0.0	0.0	0.0	8.946096780082	0.0
25	1.0255169117993925E-4	0.0	0.0	9.186785599281318	0.0
26	1.0255169117993925E-4	0.0	0.0	9.406758976862287	0.0
27	1.0255169117993925E-4	0.0	0.0	9.641294694590808	0.0
28	1.0255169117993925E-4	0.0	0.0	9.90956991871753	0.0
29	2.051033823598785E-4	0.0	0.0	10.192920241447702	0.0
30	2.051033823598785E-4	0.0	0.0	10.512881517929111	0.0
31	3.0765507353981773E-4	0.0	0.0	10.793462944997426	0.0
32	3.0765507353981773E-4	0.0	0.0	11.083991886110194	0.0
33	3.0765507353981773E-4	0.0	0.0	11.414721090165498	0.0
34	3.0765507353981773E-4	0.0	0.0	11.735195125102807	0.0
35	3.0765507353981773E-4	0.0	0.0	12.096792388203275	0.0
36	3.0765507353981773E-4	0.0	0.0	12.388859604683741	0.0
37	3.0765507353981773E-4	0.0	0.0	12.703693296606154	0.0
38	3.0765507353981773E-4	0.0	0.0	13.019142298675648	0.0
39	3.0765507353981773E-4	0.0	0.0	13.392327902879446	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	35	1.2116652E-7	45.000004	38
ATACCGG	30	2.1651613E-6	45.000004	2
CGACACT	20	7.0327614E-4	45.0	43
CCGTACG	20	7.0327614E-4	45.0	1
ACGGAAC	20	7.0327614E-4	45.0	13
TGCGTAA	20	7.0327614E-4	45.0	1
GCTACGA	200	0.0	43.875	10
AGCTACG	225	0.0	39.0	9
CGAATAT	215	0.0	38.720932	14
TAAACGG	105	0.0	38.57143	2
ATCGGCC	35	6.2473136E-6	38.57143	13
CCCTCGT	135	0.0	38.333336	14
CTAACGC	130	0.0	38.07692	25
CGTTTAG	30	1.1397948E-4	37.500004	14
CCGTTTA	30	1.1397948E-4	37.500004	13
TACGGGT	90	0.0	37.5	4
CGAGGGA	300	0.0	37.5	4
ATAGGGT	290	0.0	37.241383	4
TACGAAT	230	0.0	37.173916	12
TTAACGG	55	2.748493E-9	36.81818	2
>>END_MODULE