##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552701_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	379569
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.692443271183894	31.0	31.0	34.0	30.0	34.0
2	31.81806469969887	33.0	31.0	34.0	30.0	34.0
3	31.8495583148255	33.0	31.0	34.0	30.0	34.0
4	35.551733676880886	37.0	35.0	37.0	33.0	37.0
5	35.43898737778902	37.0	35.0	37.0	33.0	37.0
6	35.37322067924409	37.0	35.0	37.0	33.0	37.0
7	35.70802673558694	37.0	35.0	37.0	35.0	37.0
8	35.418701211110495	37.0	35.0	37.0	33.0	37.0
9	37.325627224562595	39.0	37.0	39.0	34.0	39.0
10	36.851352455021356	39.0	37.0	39.0	32.0	39.0
11	36.76333156817338	39.0	37.0	39.0	32.0	39.0
12	36.554684392034126	39.0	35.0	39.0	32.0	39.0
13	36.313658386222265	39.0	35.0	39.0	32.0	39.0
14	37.4106236283785	39.0	36.0	41.0	32.0	41.0
15	37.5731843222181	39.0	36.0	41.0	32.0	41.0
16	37.69236686873796	40.0	36.0	41.0	33.0	41.0
17	37.59179753878741	39.0	36.0	41.0	33.0	41.0
18	37.58408089174827	39.0	36.0	41.0	33.0	41.0
19	37.48708140021972	39.0	36.0	41.0	33.0	41.0
20	37.315560543669264	39.0	35.0	41.0	32.0	41.0
21	37.19009455461326	39.0	35.0	41.0	32.0	41.0
22	37.26634683022059	39.0	35.0	41.0	32.0	41.0
23	33.21513611490928	38.0	34.0	40.0	0.0	41.0
24	34.85896635394355	38.0	34.0	40.0	18.0	41.0
25	35.961435206774	38.0	34.0	40.0	27.0	41.0
26	36.52120694788036	38.0	35.0	40.0	30.0	41.0
27	30.356501716420468	38.0	31.0	40.0	0.0	41.0
28	32.931906978704795	37.0	31.0	40.0	16.0	41.0
29	34.83331884321428	37.0	31.0	40.0	25.0	41.0
30	35.83853792064157	38.0	34.0	40.0	30.0	41.0
31	36.251761866748865	38.0	35.0	40.0	30.0	41.0
32	34.675202664074256	38.0	34.0	40.0	24.0	41.0
33	35.2527946170525	38.0	34.0	40.0	24.0	41.0
34	35.628541846146554	38.0	34.0	40.0	27.0	41.0
35	35.775537517552806	38.0	35.0	40.0	29.0	41.0
36	35.809850119477616	38.0	35.0	40.0	29.0	41.0
37	35.80811393975799	38.0	35.0	40.0	29.0	41.0
38	35.787332474464456	38.0	35.0	40.0	29.0	41.0
39	35.78021914329147	38.0	35.0	40.0	29.0	41.0
40	35.64472335728155	38.0	35.0	40.0	28.0	41.0
41	35.55679731484921	38.0	35.0	40.0	28.0	41.0
42	35.48427031712284	38.0	35.0	40.0	28.0	41.0
43	35.44154817701129	38.0	34.0	40.0	28.0	41.0
44	35.3972742768772	38.0	34.0	40.0	28.0	41.0
45	35.351021816850164	38.0	34.0	40.0	27.0	41.0
46	35.21776541287618	37.0	34.0	40.0	27.0	41.0
47	35.073404308571035	37.0	34.0	40.0	27.0	41.0
48	35.068193134845046	37.0	34.0	40.0	27.0	41.0
49	35.0433254559777	37.0	34.0	40.0	27.0	41.0
50	34.8921671685517	37.0	34.0	40.0	26.0	41.0
51	34.21485421622946	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	29.0
11	22.0
12	31.0
13	21.0
14	25.0
15	35.0
16	66.0
17	99.0
18	228.0
19	449.0
20	839.0
21	1382.0
22	1982.0
23	2409.0
24	2974.0
25	3804.0
26	4579.0
27	5510.0
28	6508.0
29	8134.0
30	10467.0
31	12722.0
32	16216.0
33	21806.0
34	32233.0
35	38425.0
36	33028.0
37	42075.0
38	58749.0
39	74651.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.173341342417324	18.37189022285803	21.857422497622302	14.597345937102347
2	31.402459104932174	27.173188537525455	23.770908583156157	17.65344377438621
3	30.17580466265686	27.08914584700015	26.40890062149438	16.326148868848612
4	26.28639325129291	25.66726998253282	28.378502986281813	19.667833779892458
5	26.910522197545113	28.95283861432309	24.805239627050682	19.331399561081124
6	22.708124214569683	37.34630594173918	25.688083062631563	14.25748678105957
7	76.80685198211657	7.119127220610745	11.804968266639266	4.269052530633429
8	77.98898224038318	8.739122531081305	8.627153429284268	4.644741799251256
9	73.15982074405444	8.261212058940535	10.38388277230227	8.195084424702754
10	40.695631097376236	25.823236354918343	17.73880374846207	15.742328799243351
11	32.85436903435212	26.30167374047933	22.800334063108423	18.043623162060126
12	27.079397948726054	23.944526555119094	29.351448616720543	19.624626879434306
13	24.65296164860671	27.653206663347113	29.702372954587968	17.991458733458213
14	19.610663673798438	31.564484981650242	28.420919516609626	20.403931827941694
15	17.43187668118313	26.388087541395635	35.456794416825396	20.723241360595836
16	20.967465730868433	24.821573943077542	32.11168456855012	22.099275757503907
17	20.369418998917194	25.629332216276886	29.78483490485261	24.216413879953315
18	22.65701361280821	24.761242356462198	29.489499932818543	23.092244097911053
19	22.484186010975606	27.21428778430272	27.97067199903048	22.330854205691193
20	25.455977701024057	26.231857712300005	27.722232321396113	20.58993226527983
21	23.299847985478266	29.238689144793174	26.460011223255854	21.00145164647271
22	21.331562904241387	25.497603861221545	28.201986990507656	24.968846244029415
23	19.434938048154617	35.79296517892662	24.734633228741018	20.03746354417774
24	21.567093203080336	26.718198799164316	29.811444032573785	21.90326396518156
25	20.20976423259012	29.206283969449558	28.00518482805498	22.578766969905338
26	19.421501755938973	28.413279272016418	29.740047264133796	22.425171707910817
27	17.85814963814221	40.92115004123097	23.509559526726363	17.71114079390045
28	19.55639159151564	29.197326441305798	30.31596363243574	20.930318334742825
29	21.55655493467591	26.694224238544244	29.624389768395204	22.124831058384643
30	21.383727332843304	28.959951945496076	28.501800726613606	21.154519995047014
31	24.363949637615296	28.09186208568139	25.095042008172424	22.449146268530885
32	23.58543505923824	31.089735990030796	25.187515313421276	20.13731363730969
33	23.761160684882064	26.741646446364165	26.543527000360932	22.953665868392836
34	21.874547183779498	27.651099009666225	27.12023373879321	23.354120067761066
35	22.451780835631993	26.834910121743345	27.826034265179718	22.887274777444944
36	25.095568921592648	29.231839270330294	25.051044737583943	20.62154707049311
37	21.453279904312524	29.542981644971007	26.963740452987466	22.039997997729003
38	21.131862717977494	29.040833155500057	26.8264795070198	23.000824619502648
39	22.464426757717305	27.80364044482031	27.373679093919684	22.3582537035427
40	22.648056084664447	26.331971262142062	29.183626692380045	21.83634596081345
41	21.156100735307678	27.2111263037814	27.59445581699243	24.038317143918498
42	23.06932336413142	26.45052678169187	27.529118552884984	22.951031301291728
43	23.55908938822717	26.043222707860757	26.6807879463286	23.716899957583472
44	22.23390213637046	26.756663478840476	27.42004747489916	23.5893869098899
45	22.142219201251947	26.722150649815973	26.755082738579812	24.380547410352268
46	22.921787606469444	28.276545239468977	26.808300994022165	21.99336616003941
47	20.933743271974265	27.94722435183063	29.83410130964331	21.2849310665518
48	21.924340501990418	27.059375238757642	28.06340876098944	22.952875498262504
49	22.437817629996125	25.284203926031896	29.495032523730863	22.782945920241115
50	21.399007822029724	26.458693939705295	29.05848475507747	23.08381348318751
51	20.642096693881744	26.481614673484927	26.571716868342776	26.30457176429055
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2199.0
1	2275.5
2	2352.0
3	3004.5
4	3657.0
5	2602.0
6	1547.0
7	1459.5
8	1372.0
9	1470.0
10	1568.0
11	1465.0
12	1362.0
13	1349.0
14	1336.0
15	1272.5
16	1209.0
17	1151.5
18	1094.0
19	1263.5
20	1433.0
21	1457.0
22	1481.0
23	1501.0
24	1521.0
25	1976.5
26	3074.0
27	3716.0
28	4119.0
29	4522.0
30	5678.0
31	6834.0
32	7807.5
33	8781.0
34	9628.0
35	10475.0
36	10718.5
37	10962.0
38	11977.0
39	12992.0
40	14893.0
41	16794.0
42	18897.5
43	21001.0
44	23943.0
45	26885.0
46	30813.5
47	34742.0
48	33111.5
49	31481.0
50	31462.0
51	31443.0
52	28695.0
53	25947.0
54	23916.0
55	21885.0
56	20311.5
57	18738.0
58	17395.5
59	16053.0
60	14928.0
61	13803.0
62	12896.5
63	11990.0
64	10233.0
65	8476.0
66	7152.5
67	5829.0
68	4832.5
69	3836.0
70	3376.5
71	2917.0
72	2481.0
73	2045.0
74	1660.0
75	1066.5
76	858.0
77	613.0
78	368.0
79	271.5
80	175.0
81	122.0
82	69.0
83	51.5
84	34.0
85	24.5
86	15.0
87	36.5
88	58.0
89	32.0
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	379569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.21396353417301
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4983069164639	24.3209970589877
2	9.2014410003679	5.92829769695392
3	3.802541296933854	3.6748478002984246
4	2.0331941304710996	2.6198896630756248
5	1.27970506495735	2.0612186148516285
6	0.8398508365573317	1.623295453380155
7	0.6025220063942964	1.3586735359765834
8	0.4801990426042622	1.2375291558078796
9	0.42602016020840433	1.2351418115198474
>10	5.274760465907388	40.27147389547549
>50	0.5105665895050823	10.325089918031901
>100	0.04432571677375313	2.8558140475515286
>500	0.0024625398207640628	0.6399138884328425
>1k	0.004104233034606771	1.8478174596564891
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1546	0.4073040738311084	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	1541	0.40598679028055507	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1406	0.3704201344156135	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	1270	0.3345900218405613	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	1225	0.32273446988558074	RNA PCR Primer, Index 38 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	913	0.24053597633104917	RNA PCR Primer, Index 27 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	872	0.22973425121651136	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	635	0.16729501092028065	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	449	0.1182920628396945	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	400	0.10538268404427127	RNA PCR Primer, Index 38 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	394	0.10380194378360719	No Hit
TATATATATATATATATATATATATATATATATATATATATATATATATAT	391	0.10301157365327515	No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	385	0.1014308333926111	RNA PCR Primer, Index 38 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12461502388235077	0.0
2	0.0	0.0	0.0	0.6433612860902761	0.0
3	0.0	0.0	0.0	0.8275175264576401	0.0
4	0.0	0.0	0.0	1.1217986716512676	0.0
5	0.0	0.0	0.0	2.0789368994833612	0.0
6	0.0	0.0	0.0	2.5644876162173413	0.0
7	0.0	0.0	0.0	3.0740128935713926	0.0
8	0.0	0.0	0.0	3.8443603139350158	0.0
9	0.0	0.0	0.0	4.158400712386944	0.0
10	0.0	0.0	0.0	5.2664996351124564	0.0
11	0.0	0.0	0.0	6.154348748185442	0.0
12	0.0	0.0	0.0	7.283787664429919	0.0
13	0.0	0.0	0.0	7.564895974118013	0.0
14	0.0	0.0	0.0	7.6929359352318025	0.0
15	0.0	0.0	0.0	7.954548448371706	0.0
16	0.0	0.0	0.0	8.374761900998237	0.0
17	0.0	0.0	0.0	8.831859293040264	0.0
18	0.0	0.0	0.0	9.38643566782324	0.0
19	0.0	0.0	0.0	9.755538518688303	0.0
20	0.0	0.0	0.0	10.03849102534717	0.0
21	0.0	0.0	0.0	10.358854384841754	0.0
22	0.0	0.0	0.0	10.682379224857668	0.0
23	0.0	0.0	0.0	11.004850238033137	0.0
24	0.0	0.0	0.0	11.249338065015847	0.0
25	0.0	0.0	0.0	11.473539725320034	0.0
26	0.0	0.0	0.0	11.683778179988355	0.0
27	0.0	0.0	0.0	11.91509317146553	0.0
28	0.0	0.0	0.0	12.185399756039086	0.0
29	0.0	0.0	0.0	12.449646836280097	0.0
30	0.0	0.0	0.0	12.778704267208333	0.0
31	0.0	0.0	0.0	13.077464176473843	0.0
32	0.0	0.0	0.0	13.39545642557743	0.0
33	0.0	0.0	0.0	13.719508179013566	0.0
34	0.0	0.0	0.0	14.017741174858854	0.0
35	0.0	0.0	0.0	14.359707984582514	0.0
36	0.0	0.0	0.0	14.663737028050235	0.0
37	0.0	0.0	0.0	14.9688198983584	0.0
38	0.0	0.0	0.0	15.304463747039405	0.0
39	0.0	0.0	0.0	15.64063450914063	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGAT	25	3.8858525E-5	45.0	27
AATCCGA	25	3.8858525E-5	45.0	34
GGTACGA	35	1.2090459E-7	45.0	9
CGAGTTA	40	6.7939254E-9	45.0	18
ACGCATT	50	2.1827873E-11	45.0	17
CGTGCGG	25	3.8858525E-5	45.0	2
TTAGCAG	20	7.0273096E-4	45.0	1
CGTTGCC	20	7.0273096E-4	45.0	33
ATTACGG	20	7.0273096E-4	45.0	2
CCGTAGA	35	1.2090459E-7	45.0	27
CGCTCGT	35	1.2090459E-7	45.0	13
TCGTGAC	20	7.0273096E-4	45.0	35
TGACCGC	20	7.0273096E-4	45.0	38
CGTAGAC	20	7.0273096E-4	45.0	28
GCTTAAG	20	7.0273096E-4	45.0	1
TCCACGT	20	7.0273096E-4	45.0	13
TTCGGTG	20	7.0273096E-4	45.0	1
CGTAAGC	20	7.0273096E-4	45.0	43
CGTCGAA	20	7.0273096E-4	45.0	42
GCGGACT	20	7.0273096E-4	45.0	13
>>END_MODULE