##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552699_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773542
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90003774843512	31.0	31.0	34.0	30.0	34.0
2	32.035805424915516	33.0	31.0	34.0	30.0	34.0
3	32.059722678277325	33.0	31.0	34.0	30.0	34.0
4	35.72334533871464	37.0	35.0	37.0	35.0	37.0
5	35.60027768369397	37.0	35.0	37.0	33.0	37.0
6	35.55098494975063	37.0	35.0	37.0	33.0	37.0
7	35.77320042092091	37.0	35.0	37.0	35.0	37.0
8	35.51875528413454	37.0	35.0	37.0	33.0	37.0
9	37.36386259569616	39.0	37.0	39.0	34.0	39.0
10	36.99034570844246	39.0	37.0	39.0	33.0	39.0
11	36.88558733720988	39.0	37.0	39.0	33.0	39.0
12	36.80840859319856	39.0	35.0	39.0	33.0	39.0
13	36.63927750529383	39.0	35.0	39.0	32.0	39.0
14	37.838820645808504	40.0	37.0	41.0	33.0	41.0
15	37.97620814383705	40.0	37.0	41.0	33.0	41.0
16	38.047309648344886	40.0	37.0	41.0	33.0	41.0
17	37.92211670471674	40.0	37.0	41.0	33.0	41.0
18	37.9680056157261	40.0	37.0	41.0	33.0	41.0
19	37.90137057845599	40.0	37.0	41.0	33.0	41.0
20	37.647840711945825	40.0	36.0	41.0	33.0	41.0
21	37.57472509572848	40.0	36.0	41.0	33.0	41.0
22	37.61829868320014	40.0	36.0	41.0	33.0	41.0
23	33.43829940714273	39.0	34.0	41.0	0.0	41.0
24	35.16124787018675	39.0	35.0	41.0	17.0	41.0
25	36.291774719407606	39.0	34.0	41.0	29.0	41.0
26	36.829558058903075	39.0	35.0	41.0	31.0	41.0
27	30.61105537902273	38.0	31.0	40.0	0.0	41.0
28	33.19568814621572	38.0	31.0	40.0	16.0	41.0
29	35.14436190924346	38.0	32.0	40.0	25.0	41.0
30	36.1590101119267	38.0	35.0	40.0	30.0	41.0
31	36.570302582148095	39.0	35.0	40.0	30.0	41.0
32	34.92494525184153	39.0	35.0	40.0	24.0	41.0
33	35.52038156945583	39.0	35.0	41.0	24.0	41.0
34	35.93385362397905	39.0	35.0	41.0	28.0	41.0
35	36.07414852716465	39.0	35.0	41.0	30.0	41.0
36	36.08318746751954	39.0	35.0	41.0	29.0	41.0
37	35.99787471139253	39.0	35.0	41.0	29.0	41.0
38	35.98539704372872	39.0	35.0	41.0	29.0	41.0
39	35.866798441455025	39.0	35.0	41.0	29.0	41.0
40	35.83181391572791	38.0	35.0	40.0	28.0	41.0
41	35.84346809869406	38.0	35.0	40.0	28.0	41.0
42	35.69343228939088	38.0	35.0	40.0	28.0	41.0
43	35.56954632069105	38.0	35.0	40.0	28.0	41.0
44	35.586972653068614	38.0	35.0	40.0	28.0	41.0
45	35.494394615935526	38.0	35.0	40.0	28.0	41.0
46	35.36279736588317	38.0	34.0	40.0	27.0	41.0
47	35.2966406478252	38.0	34.0	40.0	27.0	41.0
48	35.248344627699595	38.0	34.0	40.0	27.0	41.0
49	35.22743302884653	37.0	34.0	40.0	27.0	41.0
50	35.070872686938785	37.0	34.0	40.0	27.0	41.0
51	34.396760615454625	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	55.0
10	70.0
11	65.0
12	51.0
13	59.0
14	54.0
15	61.0
16	106.0
17	143.0
18	341.0
19	835.0
20	1958.0
21	3204.0
22	4791.0
23	6111.0
24	6893.0
25	7751.0
26	8721.0
27	9950.0
28	11511.0
29	13846.0
30	17495.0
31	22228.0
32	29085.0
33	39797.0
34	59913.0
35	69558.0
36	69742.0
37	88441.0
38	123332.0
39	177255.0
40	101.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.978682476193924	17.55961537964325	21.94696603416492	15.514736109997907
2	29.898829022858486	27.59914781614961	25.04750873255751	17.454514428434397
3	30.632984375767574	26.987287050993995	26.267481274449224	16.112247298789207
4	27.998221169632675	25.10490703801474	28.408412212911514	18.488459579441063
5	27.41544738359391	29.574347611377277	23.725408575099994	19.28479642992882
6	24.72173456644888	37.97919182151713	22.977162196752083	14.32191141528191
7	78.9224114527718	6.371599732141241	10.808721439818395	3.89726737526857
8	81.20890656228103	8.230451610901541	6.909127106220477	3.6515147205969427
9	75.75981653226329	7.451825498809372	8.188178534585065	8.600179434342285
10	43.66976841593605	25.02113653815824	16.280822502204146	15.02827254370157
11	32.90047599225382	24.22803157423902	23.006119900406183	19.865372533100984
12	25.86905429827986	22.632513813083193	29.147609308867523	22.350822579769424
13	25.80855338171683	23.669303024270175	29.78506661564595	20.737076978367046
14	20.807919931949396	29.41740719960907	28.037779461231583	21.73689340720995
15	20.374976407228047	24.554193566735872	31.69821418875769	23.372615837278392
16	22.762694204063905	23.56743395962986	29.617008514081977	24.052863322224262
17	22.8663731251826	26.173627288498878	27.75660532976878	23.203394256549743
18	23.31508825635841	24.332098321745942	28.193556393835113	24.159257028060534
19	24.51541092791342	25.392932769002847	26.440581119060113	23.65107518402362
20	27.75220996403557	23.843178521657517	26.833190699406106	21.571420814900808
21	24.637188413815927	27.566699675001487	26.473675637521943	21.322436273660642
22	23.509389276858915	22.71654286386518	29.086074188602556	24.687993670673343
23	22.175654327754664	33.85065064340398	23.704078123747642	20.269616905093713
24	22.378875355184334	27.45448857334185	28.07578644727759	22.09084962419623
25	22.81362873638406	28.79429946919495	26.427653572785964	21.964418221635025
26	21.59119996070026	25.09042818618769	29.78519589110869	23.533175962003355
27	19.49486905688379	38.643538424545795	23.565753378614218	18.2958391399562
28	20.521057680125963	28.69992838139364	29.028288056757102	21.750725881723294
29	22.052325536299257	24.891731799953977	30.05357175176008	23.00237091198668
30	21.27033826217581	28.245783680782687	28.007141176561838	22.476736880479663
31	23.797415007847018	26.460101713934087	25.079569047317406	24.66291423090149
32	25.01195798030359	29.52354235451986	25.677726613422415	19.78677305175414
33	25.51677866230922	26.191725853282694	25.244912364163806	23.04658312024428
34	22.579640148821913	26.245504445783162	26.256234309190713	24.918621096204213
35	25.467783261930187	24.945381116991708	26.76532108146681	22.821514539611297
36	23.768328028730178	29.38405413022176	25.83221079139853	21.015407049649536
37	22.99965612726911	27.234591011218523	27.18145879603176	22.584294065480606
38	22.964622476866158	27.69041629284512	25.881981844554012	23.462979385734712
39	23.603889640122965	27.6564168461441	26.1481600223388	22.591533491394134
40	22.941352893572684	26.163285251479557	27.32922064994532	23.566141205002445
41	21.637997678212688	25.40508466250055	26.58485253547965	26.372065123807108
42	23.08989040026269	26.336255820627706	26.630228222901923	23.943625556207678
43	23.156725814500053	25.849662978868633	26.863441157687625	24.130170048943693
44	22.70529589860667	25.49635313919606	28.084577178744013	23.713773783453256
45	22.83379570857174	26.67030361635179	26.61536154468665	23.880539130389817
46	23.500857096317976	27.40756158036668	27.24195971259479	21.849621610720554
47	22.51461459106293	27.707480653927004	27.917424005419228	21.860480749590845
48	21.70276468504619	27.018313162051967	27.61401449436488	23.66490765853696
49	22.68215559077594	26.54477714202978	28.282627187664016	22.490440079530263
50	22.397491021819114	26.400893551998468	28.476411106313552	22.725204319868865
51	22.089427594106073	26.14712581863687	27.2821643815074	24.481282205749654
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1180.0
1	1726.0
2	2272.0
3	3631.5
4	4991.0
5	3603.0
6	2215.0
7	2151.5
8	2088.0
9	2122.0
10	2156.0
11	2153.5
12	2151.0
13	2003.5
14	1856.0
15	1854.0
16	1852.0
17	1820.0
18	1788.0
19	1874.0
20	1960.0
21	2159.0
22	2358.0
23	2997.5
24	3637.0
25	4654.5
26	6626.5
27	7581.0
28	8622.0
29	9663.0
30	12072.0
31	14481.0
32	16140.0
33	17799.0
34	20544.0
35	23289.0
36	26058.5
37	28828.0
38	28800.0
39	28772.0
40	32144.0
41	35516.0
42	37498.0
43	39480.0
44	42010.0
45	44540.0
46	49721.5
47	54903.0
48	54971.0
49	55039.0
50	54736.5
51	54434.0
52	52942.0
53	51450.0
54	49966.0
55	48482.0
56	45531.5
57	42581.0
58	40416.5
59	38252.0
60	36475.5
61	34699.0
62	31946.5
63	29194.0
64	26624.5
65	24055.0
66	20526.5
67	16998.0
68	14672.5
69	12347.0
70	10592.5
71	8838.0
72	7562.0
73	6286.0
74	5361.5
75	3624.5
76	2812.0
77	2127.0
78	1442.0
79	1011.5
80	581.0
81	456.0
82	331.0
83	212.5
84	94.0
85	106.5
86	119.0
87	73.0
88	27.0
89	18.5
90	10.0
91	7.0
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	773542.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.65532535399486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18199837465903	19.240346513780647
2	9.366904031675766	4.993557490479275
3	4.1436498971254085	3.313510084827753
4	2.363222260258564	2.5196983292398056
5	1.5248333787636192	2.032246491078778
6	1.0292808172652574	1.6461489038898702
7	0.7559646168797666	1.4105337973326777
8	0.5718795964545853	1.2194909365446607
9	0.4509777834038021	1.0818863589646595
>10	6.745563237970656	44.66070877567923
>50	0.7971249493018785	13.375573016748485
>100	0.06473620800268064	3.248568682553575
>500	0.0014493180896122532	0.23179227132026936
>1k	0.002415530149353755	1.0259383475603254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2083	0.2692807888905838	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGC	1950	0.2520871523459618	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCGTCTTC	1366	0.1765902821049148	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGACGGTCGTATGCCG	1331	0.17206564090896165	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1237	0.15991374741125886	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05183946055934907	0.0
2	0.0	0.0	0.0	0.2162778491665611	0.0
3	0.0	0.0	0.0	0.26591962685930437	0.0
4	0.0	0.0	0.0	0.3710205780681592	0.0
5	0.0	0.0	0.0	0.7804359685705495	0.0
6	0.0	0.0	0.0	0.9619387182596421	0.0
7	0.0	0.0	0.0	1.1645133683756022	0.0
8	0.0	0.0	0.0	1.4626225854575448	0.0
9	0.0	0.0	0.0	1.5787119509994285	0.0
10	0.0	0.0	0.0	2.1018897487143557	0.0
11	0.0	0.0	0.0	2.43761812545408	0.0
12	0.0	0.0	0.0	3.0145745156694788	0.0
13	0.0	0.0	0.0	3.1360934506465066	0.0
14	0.0	0.0	0.0	3.199696978315334	0.0
15	0.0	0.0	0.0	3.31850112857479	0.0
16	0.0	0.0	0.0	3.4843615472721585	0.0
17	0.0	0.0	0.0	3.6547466071654804	0.0
18	0.0	0.0	0.0	3.889381572041337	0.0
19	0.0	0.0	0.0	4.090792742992623	0.0
20	1.292754627415189E-4	0.0	0.0	4.2170948700910875	0.0
21	1.292754627415189E-4	0.0	0.0	4.33473554118587	0.0
22	1.292754627415189E-4	0.0	0.0	4.47512869372316	0.0
23	3.8782638822455664E-4	0.0	0.0	4.6089287976606315	0.0
24	3.8782638822455664E-4	0.0	0.0	4.724501061351549	0.0
25	3.8782638822455664E-4	0.0	0.0	4.822879688497845	0.0
26	3.8782638822455664E-4	0.0	0.0	4.917509327224637	0.0
27	3.8782638822455664E-4	0.0	0.0	5.042130873307461	0.0
28	3.8782638822455664E-4	0.0	0.0	5.165588940225612	0.0
29	3.8782638822455664E-4	0.0	0.0	5.300164696939532	0.0
30	3.8782638822455664E-4	0.0	0.0	5.4710668586838205	0.0
31	3.8782638822455664E-4	0.0	0.0	5.608874501966279	0.0
32	3.8782638822455664E-4	0.0	0.0	5.753663020236781	0.0
33	3.8782638822455664E-4	0.0	0.0	5.912413288483366	0.0
34	3.8782638822455664E-4	0.0	0.0	6.069224424788828	0.0
35	3.8782638822455664E-4	0.0	0.0	6.254605438360167	0.0
36	3.8782638822455664E-4	0.0	0.0	6.402884394124689	0.0
37	3.8782638822455664E-4	0.0	0.0	6.577794095213964	0.0
38	3.8782638822455664E-4	0.0	0.0	6.749989011585667	0.0
39	5.171018509660756E-4	0.0	0.0	6.920891173329955	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAGC	55	1.8189894E-12	45.000004	43
TTAGGCG	50	2.1827873E-11	45.0	1
TCGTATA	20	7.0318556E-4	45.0	40
TCGTACT	20	7.0318556E-4	45.0	25
ATGCGAT	20	7.0318556E-4	45.0	13
CCGCGTA	40	6.8102963E-9	45.0	40
CGGTATC	20	7.0318556E-4	45.0	42
CGATCGC	25	3.8896185E-5	45.0	34
GTATGCG	65	0.0	44.999996	1
TAATACG	185	0.0	42.567566	26
GTCGAAT	275	0.0	42.545456	43
TATTGCG	90	0.0	42.5	1
CGGGCTA	85	0.0	42.352943	6
CGAATAT	175	0.0	41.142857	14
GGTCGAA	290	0.0	41.120693	42
CGTGTAC	115	0.0	41.086956	18
TTAACGG	55	6.002665E-11	40.909092	2
GATGAAT	1730	0.0	40.70809	20
TCGTTTA	50	1.0804797E-9	40.5	38
CCGATGA	1600	0.0	40.5	18
>>END_MODULE