FastQCFastQC Report
Sat 18 Jun 2016
SRR3552698_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552698_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661975
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.28792628120397296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14720.2223648929340232No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7200.108765436761207No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC6650.10045696589750368No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTTA207.031117E-445.021
TCTAGCG207.031117E-445.01
AACGCGT453.8380676E-1045.027
TATAGCG207.031117E-445.01
CGATCGA207.031117E-445.041
ATCGCTA207.031117E-445.029
GTTATTC207.031117E-445.027
GTACGAC207.031117E-445.07
GTTCACG302.1641008E-644.9999961
TACGCGG556.002665E-1140.909092
ACGGGTA403.4552613E-739.3755
TCGAAAG403.4552613E-739.3751
TACGAAT950.037.89473712
CATACGA950.037.89473718
TTACGGG1900.037.8947373
AACGACC301.13939896E-437.49999627
TCCCGAT301.13939896E-437.49999639
TCGACGG301.13939896E-437.4999962
TAATGCG301.13939896E-437.4999961
ATAATAC1100.036.8181825