##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552698_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661975
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7799131387137	31.0	31.0	34.0	30.0	34.0
2	31.904707881717588	33.0	31.0	34.0	30.0	34.0
3	31.932988405906567	33.0	31.0	34.0	30.0	34.0
4	35.61053211979304	37.0	35.0	37.0	33.0	37.0
5	35.49579969032063	37.0	35.0	37.0	33.0	37.0
6	35.431270063068844	37.0	35.0	37.0	33.0	37.0
7	35.73623173080554	37.0	35.0	37.0	35.0	37.0
8	35.52974054911439	37.0	35.0	37.0	35.0	37.0
9	37.34895124438234	39.0	37.0	39.0	34.0	39.0
10	36.90262774273953	39.0	37.0	39.0	33.0	39.0
11	36.77934363080177	39.0	37.0	39.0	32.0	39.0
12	36.60670115940934	39.0	35.0	39.0	32.0	39.0
13	36.47072925714717	39.0	35.0	39.0	32.0	39.0
14	37.560291551795764	40.0	36.0	41.0	32.0	41.0
15	37.712644737339026	40.0	36.0	41.0	33.0	41.0
16	37.77162581668492	40.0	36.0	41.0	33.0	41.0
17	37.69392046527437	40.0	36.0	41.0	33.0	41.0
18	37.669164243362665	39.0	36.0	41.0	33.0	41.0
19	37.612217984062845	39.0	36.0	41.0	33.0	41.0
20	37.48737036897164	39.0	35.0	41.0	32.0	41.0
21	37.34819592884928	39.0	35.0	41.0	32.0	41.0
22	37.39967068242758	39.0	35.0	41.0	32.0	41.0
23	33.52745345368027	39.0	34.0	41.0	5.0	41.0
24	35.06913856263454	39.0	34.0	41.0	18.0	41.0
25	36.084500169946	38.0	34.0	41.0	28.0	41.0
26	36.63879300577816	38.0	35.0	40.0	30.0	41.0
27	30.73048680086106	38.0	31.0	40.0	0.0	41.0
28	33.13830431662827	38.0	31.0	40.0	16.0	41.0
29	34.98036028550927	38.0	32.0	40.0	25.0	41.0
30	35.9370988330375	38.0	34.0	40.0	30.0	41.0
31	36.310125004720724	38.0	35.0	40.0	30.0	41.0
32	34.817429661240986	38.0	34.0	40.0	25.0	41.0
33	35.38771403753918	38.0	34.0	40.0	25.0	41.0
34	35.77241738736357	38.0	34.0	40.0	27.0	41.0
35	35.909528305449605	38.0	35.0	40.0	29.0	41.0
36	35.9299233354734	38.0	35.0	40.0	29.0	41.0
37	35.96217077684202	38.0	35.0	40.0	30.0	41.0
38	35.89811397711394	38.0	35.0	40.0	29.0	41.0
39	35.82246459458439	38.0	35.0	40.0	29.0	41.0
40	35.71170965670909	38.0	35.0	40.0	29.0	41.0
41	35.630096302730465	38.0	34.0	40.0	28.0	41.0
42	35.55475811020054	38.0	34.0	40.0	28.0	41.0
43	35.50361569545678	38.0	34.0	40.0	28.0	41.0
44	35.43603610408248	38.0	34.0	40.0	28.0	41.0
45	35.336079157067864	37.0	34.0	40.0	28.0	41.0
46	35.21463046187545	37.0	34.0	40.0	28.0	41.0
47	35.10216246837116	37.0	34.0	40.0	27.0	41.0
48	35.04499263567355	37.0	34.0	40.0	27.0	41.0
49	34.98666414894822	37.0	34.0	40.0	27.0	41.0
50	34.86346916424336	36.0	34.0	40.0	27.0	41.0
51	34.119386683787155	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	36.0
10	84.0
11	58.0
12	43.0
13	50.0
14	59.0
15	81.0
16	121.0
17	196.0
18	367.0
19	667.0
20	1145.0
21	1825.0
22	2825.0
23	3646.0
24	4914.0
25	6176.0
26	7662.0
27	9278.0
28	11191.0
29	14149.0
30	17606.0
31	22261.0
32	27965.0
33	37850.0
34	56363.0
35	62273.0
36	61764.0
37	76780.0
38	103884.0
39	130583.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.337814872162845	17.577854148570566	22.485139166886967	14.599191812379622
2	30.840892782960083	25.223157974243744	25.619849692208916	18.316099550587257
3	31.714037539181994	25.38736357113184	26.391782167000265	16.5068167226859
4	28.494580611050267	26.254918992409078	26.51580497752936	18.73469541901129
5	26.260961516673593	30.171532157558822	24.482948751841082	19.08455757392651
6	25.598549794176517	35.9019600438083	23.771743645908078	14.727746516107104
7	80.98931228520715	6.467011594093433	8.418746931530647	4.124929189168776
8	83.0147664186714	6.073643264473734	6.870199025642962	4.041391291211903
9	77.1861475131236	7.471430189961857	9.207900600475849	6.134521696438687
10	42.216397900222816	26.807809962611884	15.69001850523056	15.28577363193474
11	33.53797348842479	25.433286755542127	22.604630084217682	18.4241096718154
12	28.2492541259111	23.176555005853697	28.312247441368633	20.261943426866573
13	26.752822991804827	23.62400392764077	29.28600022659466	20.337172853959743
14	22.805997205332527	26.641791608444425	28.7997280864081	21.75248309981495
15	21.94221836172061	24.48612107707995	31.911174893311685	21.66048566788776
16	25.610634842705537	23.29393103969183	29.639034706748745	21.456399410853884
17	25.846746478341327	23.78926696627516	26.965217719702405	23.398768835681103
18	26.634087390007178	23.488651384115713	27.637750670342538	22.239510555534576
19	26.669738283167792	25.534196910759473	25.287812983874012	22.508251822198723
20	27.444238830771557	24.943842290116695	26.283469919558893	21.328448959552855
21	26.571547263869483	26.59994712791269	26.566260055138034	20.2622455530798
22	25.55066278938026	23.81464556818611	27.53019373843423	23.104497903999395
23	22.57894935609351	33.31107670229238	24.52947618867782	19.58049775293629
24	24.542920805166357	24.98311869783602	28.083537897956873	22.39042259904075
25	25.405037954605536	26.024849881037802	25.915631254956757	22.6544809093999
26	23.444389893878167	25.7885871822954	26.720646550096305	24.046376373730123
27	20.043506174704483	37.69704293968805	22.52713471052532	19.73231617508214
28	22.810982287850752	26.956909248838702	27.5605574228634	22.671551040447145
29	24.058461422259146	26.03617961403376	27.05766834094943	22.847690622757657
30	24.885078741644325	25.26741946448129	27.603912534461273	22.243589259413117
31	26.20582348275992	25.478605687525963	25.461686619585333	22.85388421012878
32	25.73964273575286	28.854412931001928	24.833717285395974	20.57222704784924
33	26.018203104346842	25.586011556327655	25.431171872049546	22.964613467275953
34	25.15487745005476	26.126817478001435	26.78001435099513	21.938290720948675
35	24.88266173193852	25.658068658181953	27.048151365232826	22.4111182446467
36	25.192643226707958	28.53808678575475	24.944899731862986	21.324370255674307
37	24.71754975641074	27.71705880131425	25.333131915857848	22.23225952641716
38	24.430076664526606	27.876279315684126	24.96468899882926	22.728955020960008
39	25.072396993844176	26.160957740095924	25.008950489066805	23.757694776993088
40	25.21515162959326	25.038860984176143	27.380037010461123	22.36595037576948
41	22.841648098493145	26.37048226896786	26.18935760413913	24.598512028399863
42	23.71826730616715	26.050530609161974	26.43332452131878	23.797877563352092
43	23.99244684466936	25.28025982854337	26.824729030552515	23.90256429623475
44	23.996827674761132	25.122096755919788	26.952679481853544	23.92839608746554
45	24.342460062691192	25.26379394992258	26.520487933834357	23.873258053551872
46	24.829789644624043	25.898712187016127	26.39148004078704	22.880018127572793
47	23.215378224253183	26.664451074436347	27.41085388421013	22.709316817100344
48	23.62491030628045	26.225612749726196	27.152535971902264	22.99694097209109
49	24.025378601910948	25.448997318629857	27.402092224026585	23.123531855432606
50	22.18135125948865	26.095698478039203	27.826730616715135	23.896219645757014
51	22.67940632199101	25.62740284753956	26.652063899694095	25.04112693077533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1762.0
1	2182.0
2	2602.0
3	3716.5
4	4831.0
5	3438.0
6	2045.0
7	1981.5
8	1918.0
9	2025.5
10	2133.0
11	2124.0
12	2115.0
13	2118.5
14	2122.0
15	2071.0
16	2020.0
17	2069.5
18	2119.0
19	2087.5
20	2056.0
21	2281.0
22	2506.0
23	3005.5
24	3505.0
25	3769.5
26	5014.5
27	5995.0
28	6858.5
29	7722.0
30	8980.5
31	10239.0
32	11390.5
33	12542.0
34	14114.0
35	15686.0
36	16867.0
37	18048.0
38	19519.0
39	20990.0
40	22286.5
41	23583.0
42	26644.0
43	29705.0
44	31754.0
45	33803.0
46	37923.5
47	42044.0
48	44427.0
49	46810.0
50	47207.0
51	47604.0
52	47060.0
53	46516.0
54	45384.0
55	44252.0
56	42835.0
57	41418.0
58	38805.5
59	36193.0
60	34604.0
61	33015.0
62	30855.5
63	28696.0
64	25420.0
65	22144.0
66	19447.5
67	16751.0
68	14533.5
69	12316.0
70	10762.0
71	9208.0
72	8350.0
73	7492.0
74	6489.0
75	4585.5
76	3685.0
77	2892.5
78	2100.0
79	1642.0
80	1184.0
81	881.0
82	578.0
83	413.5
84	249.0
85	179.0
86	109.0
87	67.0
88	25.0
89	20.0
90	15.0
91	9.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	661975.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.26488817254349
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.82525105341495	24.225238324292537
2	9.748430478129716	6.485608994255994
3	4.065036571467711	4.05668960901519
4	2.161443785386136	2.8760074324843563
5	1.320409716961043	2.196164078832445
6	0.9478904569727218	1.8918882030611244
7	0.7432355255363896	1.7306552649980715
8	0.6427579931901882	1.7105018212384069
9	0.5118950884705648	1.5325319586642832
>10	6.851405591132645	47.57215460308262
>50	0.14821296738260287	3.0232643861475337
>100	0.030896358748963473	1.7443685872703925
>500	0.002238866576011846	0.45176927036043885
>1k	8.955466304047384E-4	0.5031574662965916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1906	0.28792628120397296	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1472	0.2223648929340232	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	720	0.108765436761207	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	665	0.10045696589750368	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06087843196495336	0.0
2	0.0	0.0	0.0	0.30348578118509006	0.0
3	0.0	0.0	0.0	0.4105895237735564	0.0
4	0.0	0.0	0.0	0.5459420672986139	0.0
5	0.0	0.0	0.0	0.9529060765134635	0.0
6	0.0	0.0	0.0	1.2745194304920882	0.0
7	0.0	0.0	0.0	1.601571056308773	0.0
8	0.0	0.0	0.0	2.1648853808678576	0.0
9	0.0	0.0	0.0	2.4245628611352394	0.0
10	0.0	0.0	0.0	3.0158238604176897	0.0
11	0.0	0.0	0.0	3.539861777257449	0.0
12	0.0	0.0	0.0	4.082178329997356	0.0
13	0.0	0.0	0.0	4.255296650175611	0.0
14	0.0	0.0	0.0	4.33868348502587	0.0
15	0.0	0.0	0.0	4.4685977567128665	0.0
16	0.0	0.0	0.0	4.6920200913931795	0.0
17	0.0	0.0	0.0	4.919219003738812	0.0
18	0.0	0.0	0.0	5.214396314060199	0.0
19	0.0	0.0	0.0	5.395369915782318	0.0
20	0.0	0.0	0.0	5.562294648589448	0.0
21	0.0	0.0	0.0	5.742663997885116	0.0
22	1.510631066127875E-4	0.0	0.0	5.930284376298198	0.0
23	1.510631066127875E-4	0.0	0.0	6.117602628498055	0.0
24	1.510631066127875E-4	0.0	0.0	6.274859322481967	0.0
25	1.510631066127875E-4	0.0	0.0	6.4067374145549305	0.0
26	1.510631066127875E-4	0.0	0.0	6.5387665697345065	0.0
27	1.510631066127875E-4	0.0	0.0	6.701310472449866	0.0
28	1.510631066127875E-4	0.0	0.0	6.885607462517466	0.0
29	1.510631066127875E-4	0.0	0.0	7.076249103062804	0.0
30	1.510631066127875E-4	0.0	0.0	7.297254428037313	0.0
31	1.510631066127875E-4	0.0	0.0	7.492579024887647	0.0
32	1.510631066127875E-4	0.0	0.0	7.702254616866196	0.0
33	1.510631066127875E-4	0.0	0.0	7.91328977680426	0.0
34	1.510631066127875E-4	0.0	0.0	8.128403640620869	0.0
35	1.510631066127875E-4	0.0	0.0	8.353034480154085	0.0
36	1.510631066127875E-4	0.0	0.0	8.561803693492957	0.0
37	1.510631066127875E-4	0.0	0.0	8.780694134974885	0.0
38	1.510631066127875E-4	0.0	0.0	9.026322746327278	0.0
39	1.510631066127875E-4	0.0	0.0	9.33857018769591	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.031117E-4	45.0	21
TCTAGCG	20	7.031117E-4	45.0	1
AACGCGT	45	3.8380676E-10	45.0	27
TATAGCG	20	7.031117E-4	45.0	1
CGATCGA	20	7.031117E-4	45.0	41
ATCGCTA	20	7.031117E-4	45.0	29
GTTATTC	20	7.031117E-4	45.0	27
GTACGAC	20	7.031117E-4	45.0	7
GTTCACG	30	2.1641008E-6	44.999996	1
TACGCGG	55	6.002665E-11	40.90909	2
ACGGGTA	40	3.4552613E-7	39.375	5
TCGAAAG	40	3.4552613E-7	39.375	1
TACGAAT	95	0.0	37.894737	12
CATACGA	95	0.0	37.894737	18
TTACGGG	190	0.0	37.894737	3
AACGACC	30	1.13939896E-4	37.499996	27
TCCCGAT	30	1.13939896E-4	37.499996	39
TCGACGG	30	1.13939896E-4	37.499996	2
TAATGCG	30	1.13939896E-4	37.499996	1
ATAATAC	110	0.0	36.81818	25
>>END_MODULE