##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552696_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	358877
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.72631848794991	31.0	31.0	34.0	30.0	34.0
2	31.84984827670762	33.0	31.0	34.0	30.0	34.0
3	31.878646444324936	33.0	31.0	34.0	30.0	34.0
4	35.56016964029459	37.0	35.0	37.0	33.0	37.0
5	35.449376805980876	37.0	35.0	37.0	33.0	37.0
6	35.38140644287597	37.0	35.0	37.0	33.0	37.0
7	35.68842249572973	37.0	35.0	37.0	35.0	37.0
8	35.393856390908304	37.0	35.0	37.0	33.0	37.0
9	37.29557759343731	39.0	37.0	39.0	34.0	39.0
10	36.855914421932866	39.0	37.0	39.0	32.0	39.0
11	36.768923614497446	39.0	37.0	39.0	32.0	39.0
12	36.59845295184701	39.0	35.0	39.0	32.0	39.0
13	36.35893913513544	39.0	35.0	39.0	32.0	39.0
14	37.44747086048981	39.0	36.0	41.0	32.0	41.0
15	37.643064336806205	40.0	36.0	41.0	33.0	41.0
16	37.72937524555767	40.0	36.0	41.0	33.0	41.0
17	37.63181257088083	39.0	36.0	41.0	33.0	41.0
18	37.609526383691346	39.0	36.0	41.0	33.0	41.0
19	37.50661368658343	39.0	36.0	41.0	33.0	41.0
20	37.30067404709692	39.0	35.0	41.0	32.0	41.0
21	37.168637165379785	39.0	35.0	41.0	32.0	41.0
22	37.234035616659746	39.0	35.0	41.0	32.0	41.0
23	33.219164226183345	38.0	34.0	40.0	0.0	41.0
24	34.845206017660644	38.0	34.0	40.0	17.0	41.0
25	35.90003538816921	38.0	34.0	40.0	27.0	41.0
26	36.45347848984471	38.0	35.0	40.0	30.0	41.0
27	30.378360831148278	37.0	31.0	40.0	0.0	41.0
28	32.810678867690044	37.0	31.0	40.0	15.0	41.0
29	34.72995761779105	37.0	31.0	40.0	25.0	41.0
30	35.72484444531135	37.0	34.0	40.0	30.0	41.0
31	36.0588808979121	38.0	35.0	40.0	30.0	41.0
32	34.50338416783466	38.0	34.0	40.0	24.0	41.0
33	35.09837911039158	38.0	34.0	40.0	24.0	41.0
34	35.473839226253006	38.0	34.0	40.0	27.0	41.0
35	35.63369343814176	38.0	35.0	40.0	29.0	41.0
36	35.672464381947016	38.0	35.0	40.0	29.0	41.0
37	35.616364381111076	38.0	35.0	40.0	29.0	41.0
38	35.58328898201891	38.0	35.0	40.0	28.0	41.0
39	35.51290553587998	38.0	35.0	40.0	28.0	41.0
40	35.417789381877355	38.0	34.0	40.0	27.0	41.0
41	35.29838078227359	38.0	34.0	40.0	27.0	41.0
42	35.2272979321606	38.0	34.0	40.0	27.0	41.0
43	35.212838939246595	38.0	34.0	40.0	27.0	41.0
44	35.15124959247876	38.0	34.0	40.0	27.0	41.0
45	35.0398270159414	37.0	34.0	40.0	26.0	41.0
46	34.92756013898912	37.0	34.0	40.0	26.0	41.0
47	34.77903292771618	37.0	34.0	40.0	26.0	41.0
48	34.759112453570445	37.0	34.0	40.0	26.0	41.0
49	34.7344577668672	37.0	34.0	40.0	26.0	41.0
50	34.570518589934714	36.0	34.0	40.0	26.0	41.0
51	33.83455612925877	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	35.0
10	29.0
11	24.0
12	21.0
13	32.0
14	33.0
15	41.0
16	61.0
17	143.0
18	247.0
19	439.0
20	844.0
21	1358.0
22	2024.0
23	2573.0
24	3230.0
25	3898.0
26	4512.0
27	5359.0
28	6218.0
29	7638.0
30	9797.0
31	12129.0
32	15490.0
33	20542.0
34	30853.0
35	37398.0
36	32516.0
37	39714.0
38	53815.0
39	67825.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.86615469924236	19.302713743148768	23.424738838097735	15.406392719511143
2	30.486768447128128	27.765501829317568	24.855312544409365	16.892417179144946
3	32.435346929449366	26.757078330458622	25.598742744728696	15.208831995363315
4	27.100650083454774	26.604937067574685	27.33889326983897	18.95551957913157
5	25.775404943755102	30.79801714793648	24.03497577164321	19.39160213666521
6	23.05441697294616	38.065966891163264	24.527623670505495	14.351992465385077
7	77.20500338556107	7.879022617777122	10.298793179835988	4.617180816825821
8	78.85765875216299	8.539416011614007	8.00831482652831	4.5946104096946865
9	74.77965988346983	8.971597511124981	9.556756214524754	6.69198639088044
10	41.30050128595592	28.742159569991948	16.064835584336695	13.892503559715447
11	32.671918233823845	26.770732033537953	22.367273466953858	18.190076265684343
12	27.81677287761545	23.500809469539703	28.585838602083726	20.096579050761125
13	25.169905009237148	26.98501157778292	28.995171047461945	18.84991236551799
14	19.35231290943694	32.6462827096749	28.056130652006118	19.94527372888204
15	18.82344090036419	26.715281280215784	34.07685641598653	20.38442140343349
16	21.206430058209357	25.495643354129687	31.980873669808872	21.317052917852077
17	21.311758624821316	24.99352145721236	28.28322795832555	25.41149195964077
18	23.662424730478687	24.677257110374864	28.409733697060553	23.250584462085897
19	25.162938834196673	25.85844175023755	26.36697252819211	22.611646887373666
20	26.849031840992875	26.09389846660555	26.24381055347654	20.813259138925037
21	24.216096322695517	28.699805225745866	26.100307347642786	20.983791103915827
22	22.71892598299696	26.016713247157103	26.464220331757122	24.800140438088818
23	19.181780944446146	36.501921271076164	24.14949968930859	20.166798095169096
24	21.38197766922929	27.353104266921534	28.646026354433413	22.618891709415763
25	21.854284336973393	28.55685931391535	27.228270410196252	22.360585938915005
26	20.824405018989793	29.871794514555127	25.91249926855162	23.39130119790346
27	19.106546254009032	41.05278410151668	22.228785907149245	17.611883737325044
28	20.002675011215544	30.11003770093932	29.259607051998316	20.62768023584682
29	21.764002708448857	27.996500193659667	27.99287778263862	22.246619315252858
30	21.25825840051048	29.00380910451213	26.606051655581158	23.131880839396228
31	24.68087952139591	28.65187794146741	24.061725883798626	22.60551665333805
32	24.553816488657674	31.72507572232269	23.889243389796505	19.831864399223132
33	24.517035084443975	27.570170281182687	23.753542300008082	24.159252334365256
34	23.051630502929974	29.0548015058084	24.453782215076476	23.439785776185154
35	22.922338294178786	28.109909523318578	25.48449747406493	23.48325470843771
36	23.37068131978366	30.583737603691514	24.120241754138604	21.925339322386222
37	24.427032102921057	29.2005338876551	25.757292888649875	20.61514112077397
38	23.232193759979044	29.622684095107793	24.0745436458731	23.07057849904006
39	23.844938516539095	27.47208653661282	24.53041014052168	24.152564806326403
40	23.559882633882918	26.220961499343787	26.943493174541693	23.2756626922316
41	19.987628073128118	28.111302758326666	26.344123474059355	25.556945694485854
42	22.56678472011302	27.384870025106096	26.026744539215386	24.0216007155655
43	22.81115814053283	26.802497791722512	25.876832452344395	24.50951161540026
44	22.46396397651563	26.881633540182293	26.529981024139186	24.12442145916289
45	22.43275551233431	28.712901634821957	25.669797730141525	23.184545122702207
46	24.272104370020926	28.26762372623489	25.12114178395383	22.339130119790347
47	21.498730762907627	27.56515463515355	27.557909813111458	23.378204788827368
48	21.898867857232425	26.27780548767405	27.613081919432002	24.210244735661522
49	22.41965910325822	25.766209592701678	28.174276980692547	23.639854323347553
50	21.516285524009618	25.892436684435054	28.03188836286527	24.559389428690054
51	21.124786486735008	26.617476182647536	26.000551721063204	26.257185609554252
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2654.0
1	2772.0
2	2890.0
3	3155.5
4	3421.0
5	2466.0
6	1511.0
7	1447.5
8	1384.0
9	1411.0
10	1438.0
11	1491.0
12	1544.0
13	1537.0
14	1530.0
15	1473.5
16	1417.0
17	1438.5
18	1460.0
19	1440.0
20	1420.0
21	1358.5
22	1297.0
23	1387.5
24	1478.0
25	1534.0
26	2237.5
27	2885.0
28	3033.5
29	3182.0
30	4531.0
31	5880.0
32	5806.0
33	5732.0
34	6811.5
35	7891.0
36	8495.5
37	9100.0
38	9276.0
39	9452.0
40	11514.0
41	13576.0
42	17048.5
43	20521.0
44	22504.0
45	24487.0
46	27507.0
47	30527.0
48	30709.0
49	30891.0
50	30380.5
51	29870.0
52	27947.0
53	26024.0
54	24117.0
55	22210.0
56	20526.0
57	18842.0
58	17510.5
59	16179.0
60	15236.0
61	14293.0
62	13067.0
63	11841.0
64	10080.5
65	8320.0
66	7117.5
67	5915.0
68	5483.5
69	5052.0
70	4281.5
71	3511.0
72	3043.0
73	2575.0
74	2374.5
75	1747.5
76	1321.0
77	1096.0
78	871.0
79	604.5
80	338.0
81	301.5
82	265.0
83	171.5
84	78.0
85	50.5
86	23.0
87	15.5
88	8.0
89	5.0
90	2.0
91	4.0
92	6.0
93	3.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	358877.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.56883949458432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0235892461333	21.296539502427848
2	10.569887978006621	6.250786421948283
3	4.276716211585357	3.7937260567276234
4	2.340164973591735	2.767838499799287
5	1.4092740775280534	2.0835299501152695
6	0.890186083937044	1.579306166174821
7	0.7059451602745688	1.4611785395296129
8	0.5496170699879341	1.3001231140765555
9	0.4520394012217397	1.2029652449958261
>10	6.223007010237422	44.050120878268764
>50	0.5008276016202865	9.14058922157539
>100	0.049784055827066256	2.3903257606200468
>500	0.005974086699247951	1.2709738032512308
>1k	0.0029870433496239756	1.4119968404894376
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2235	0.6227760486183289	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1362	0.379517216204995	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1199	0.334097754941108	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	912	0.25412606547647243	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	845	0.23545671636800353	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	771	0.21483683824820204	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	727	0.2025763701769687	Illumina PCR Primer Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	558	0.15548502690336802	Illumina PCR Primer Index 7 (96% over 27bp)
CTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGCT	504	0.1404380888159453	Illumina PCR Primer Index 7 (96% over 28bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	372	0.10365668460224534	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	371	0.10337803760062642	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16579496596326876	0.0
2	0.0	0.0	0.0	0.617481755587569	0.0
3	0.0	0.0	0.0	0.7827194275475998	0.0
4	0.0	0.0	0.0	1.0254209659576958	0.0
5	0.0	0.0	0.0	1.8020101594696791	0.0
6	0.0	0.0	0.0	2.2885278242963465	0.0
7	0.0	0.0	0.0	2.8068112473075733	0.0
8	0.0	0.0	0.0	3.6296558430883006	0.0
9	0.0	0.0	0.0	3.983816182145972	0.0
10	0.0	0.0	0.0	5.022612204181377	0.0
11	0.0	0.0	0.0	5.952735895585396	0.0
12	0.0	0.0	0.0	7.055063433989918	0.0
13	0.0	0.0	0.0	7.368819957812844	0.0
14	0.0	0.0	0.0	7.508143458622314	0.0
15	0.0	0.0	0.0	7.769514346140879	0.0
16	0.0	0.0	0.0	8.188042142572526	0.0
17	0.0	0.0	0.0	8.616601231062454	0.0
18	0.0	0.0	0.0	9.278387859907433	0.0
19	0.0	0.0	0.0	9.634220080974819	0.0
20	0.0	0.0	0.0	9.937666665737844	0.0
21	0.0	0.0	0.0	10.209626139317928	0.0
22	0.0	0.0	0.0	10.505549255037241	0.0
23	0.0	0.0	0.0	10.800915076753316	0.0
24	0.0	0.0	0.0	11.027733736071132	0.0
25	0.0	0.0	0.0	11.235325752277243	0.0
26	0.0	0.0	0.0	11.426477595387835	0.0
27	0.0	0.0	0.0	11.665835369778504	0.0
28	0.0	0.0	0.0	11.909094202191838	0.0
29	0.0	0.0	0.0	12.145386859564697	0.0
30	0.0	0.0	0.0	12.444653739303439	0.0
31	0.0	0.0	0.0	12.71995697690295	0.0
32	0.0	0.0	0.0	12.97742680639885	0.0
33	0.0	0.0	0.0	13.270563452101973	0.0
34	0.0	0.0	0.0	13.528311928599493	0.0
35	0.0	0.0	0.0	13.845412216441845	0.0
36	0.0	0.0	0.0	14.134647804122304	0.0
37	0.0	0.0	0.0	14.410508335725053	0.0
38	0.0	0.0	0.0	14.706988745447605	0.0
39	0.0	0.0	0.0	14.991208687098922	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATT	135	0.0	45.000004	17
ACAACGC	25	3.8854254E-5	45.0	39
CGGGTCG	20	7.0267956E-4	45.0	17
TCGCAAT	20	7.0267956E-4	45.0	22
CGAACCA	25	3.8854254E-5	45.0	15
CTCGTGC	20	7.0267956E-4	45.0	45
CCGATTT	20	7.0267956E-4	45.0	11
TACCAAC	20	7.0267956E-4	45.0	44
GGCCGTA	25	3.8854254E-5	45.0	42
GGCAACC	20	7.0267956E-4	45.0	29
TTCACGG	35	1.2088094E-7	45.0	2
ATTCGCT	20	7.0267956E-4	45.0	32
CGATTGT	20	7.0267956E-4	45.0	13
TACGGGT	25	3.8854254E-5	45.0	4
TAACGCC	20	7.0267956E-4	45.0	12
TCTACGA	25	3.8854254E-5	45.0	22
GTGCTCG	20	7.0267956E-4	45.0	33
CGTAGTG	25	3.8854254E-5	45.0	28
AGATTCG	20	7.0267956E-4	45.0	30
ATGACCG	20	7.0267956E-4	45.0	15
>>END_MODULE