Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552695_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1171826 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC | 3021 | 0.2578027795935574 | Illumina PCR Primer Index 10 (95% over 23bp) |
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC | 2614 | 0.22307066066122447 | Illumina PCR Primer Index 10 (95% over 23bp) |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2046 | 0.1745993005787549 | No Hit |
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT | 1901 | 0.16222544985347653 | Illumina PCR Primer Index 10 (95% over 24bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1804 | 0.1539477704027731 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATTAGCG | 35 | 1.2119563E-7 | 45.0 | 1 |
ATATGCG | 35 | 1.2119563E-7 | 45.0 | 1 |
CCGTATG | 20 | 7.033343E-4 | 45.0 | 1 |
TACGTCC | 20 | 7.033343E-4 | 45.0 | 28 |
TTCTACG | 30 | 2.1655396E-6 | 44.999996 | 1 |
ACGTCGT | 30 | 2.1655396E-6 | 44.999996 | 26 |
TAGACGT | 70 | 0.0 | 41.785713 | 26 |
CGTAGTC | 65 | 0.0 | 41.538464 | 30 |
TTCGTAG | 50 | 1.0822987E-9 | 40.500004 | 1 |
TAGTACG | 45 | 1.9283107E-8 | 40.0 | 1 |
TTGGACG | 80 | 0.0 | 39.375 | 1 |
TAACGCG | 40 | 3.458299E-7 | 39.375 | 1 |
TCGACGT | 40 | 3.458299E-7 | 39.375 | 26 |
TCGTAGG | 130 | 0.0 | 38.076927 | 2 |
CTAACGC | 255 | 0.0 | 37.941177 | 25 |
TAACGTT | 30 | 1.1399357E-4 | 37.499996 | 38 |
TCGCTAA | 30 | 1.1399357E-4 | 37.499996 | 19 |
CATCGAT | 30 | 1.1399357E-4 | 37.499996 | 16 |
TTACGAC | 30 | 1.1399357E-4 | 37.499996 | 14 |
TGTCGAC | 30 | 1.1399357E-4 | 37.499996 | 12 |