FastQCFastQC Report
Sat 18 Jun 2016
SRR3552695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171826
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC30210.2578027795935574Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC26140.22307066066122447Illumina PCR Primer Index 10 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20460.1745993005787549No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT19010.16222544985347653Illumina PCR Primer Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18040.1539477704027731No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGCG351.2119563E-745.01
ATATGCG351.2119563E-745.01
CCGTATG207.033343E-445.01
TACGTCC207.033343E-445.028
TTCTACG302.1655396E-644.9999961
ACGTCGT302.1655396E-644.99999626
TAGACGT700.041.78571326
CGTAGTC650.041.53846430
TTCGTAG501.0822987E-940.5000041
TAGTACG451.9283107E-840.01
TTGGACG800.039.3751
TAACGCG403.458299E-739.3751
TCGACGT403.458299E-739.37526
TCGTAGG1300.038.0769272
CTAACGC2550.037.94117725
TAACGTT301.1399357E-437.49999638
TCGCTAA301.1399357E-437.49999619
CATCGAT301.1399357E-437.49999616
TTACGAC301.1399357E-437.49999614
TGTCGAC301.1399357E-437.49999612