##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552695_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1171826
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68077769225124	31.0	31.0	34.0	30.0	34.0
2	31.79056190936197	31.0	31.0	34.0	30.0	34.0
3	31.830700121007727	33.0	31.0	34.0	30.0	34.0
4	35.52036991840085	37.0	35.0	37.0	33.0	37.0
5	35.41853654040788	37.0	35.0	37.0	33.0	37.0
6	35.358822043545715	37.0	35.0	37.0	33.0	37.0
7	35.7872405971535	37.0	35.0	37.0	35.0	37.0
8	35.662526689115964	37.0	35.0	37.0	35.0	37.0
9	37.45882835847643	39.0	37.0	39.0	35.0	39.0
10	36.813433052347364	39.0	37.0	39.0	32.0	39.0
11	36.73454079359905	39.0	37.0	39.0	32.0	39.0
12	36.71614045088605	39.0	35.0	39.0	32.0	39.0
13	36.60039801130885	39.0	35.0	39.0	32.0	39.0
14	37.702844961623995	40.0	36.0	41.0	33.0	41.0
15	37.825431420705804	40.0	37.0	41.0	33.0	41.0
16	37.87252885667326	40.0	37.0	41.0	33.0	41.0
17	37.835424371877735	40.0	36.0	41.0	33.0	41.0
18	37.821959062181584	39.0	36.0	41.0	33.0	41.0
19	37.766060831556906	39.0	36.0	41.0	33.0	41.0
20	37.70953878818187	39.0	36.0	41.0	33.0	41.0
21	37.5681918646625	39.0	36.0	41.0	32.0	41.0
22	37.65664868333695	39.0	36.0	41.0	33.0	41.0
23	33.68396075867919	39.0	35.0	41.0	0.0	41.0
24	35.3325604654616	39.0	35.0	41.0	18.0	41.0
25	36.34821978689669	39.0	34.0	41.0	29.0	41.0
26	36.9551742323519	39.0	35.0	41.0	31.0	41.0
27	30.853997948500886	38.0	32.0	40.0	0.0	41.0
28	33.44564295381738	38.0	31.0	40.0	16.0	41.0
29	35.34117522567344	38.0	32.0	40.0	25.0	41.0
30	36.32750766752061	38.0	35.0	40.0	30.0	41.0
31	36.74143516187557	39.0	35.0	40.0	31.0	41.0
32	35.24231925217566	39.0	35.0	40.0	27.0	41.0
33	35.94130954595648	39.0	35.0	40.0	27.0	41.0
34	36.397691295465364	39.0	35.0	40.0	30.0	41.0
35	36.593020636169534	39.0	35.0	40.0	30.0	41.0
36	36.6464679909816	39.0	35.0	40.0	31.0	41.0
37	36.663999604036775	39.0	35.0	40.0	31.0	41.0
38	36.635474891323454	39.0	35.0	40.0	31.0	41.0
39	36.612781249093295	39.0	35.0	40.0	31.0	41.0
40	36.55844809724311	39.0	35.0	40.0	30.0	41.0
41	36.51149402727026	39.0	35.0	40.0	30.0	41.0
42	36.470925717640675	39.0	35.0	40.0	30.0	41.0
43	36.41816191141005	39.0	35.0	40.0	30.0	41.0
44	36.33125566423684	39.0	35.0	40.0	30.0	41.0
45	36.236432712706495	38.0	35.0	40.0	30.0	41.0
46	36.140635213760405	38.0	35.0	40.0	30.0	41.0
47	36.07322076827106	38.0	35.0	40.0	30.0	41.0
48	36.031606228228426	38.0	35.0	40.0	30.0	41.0
49	36.02350434279492	38.0	35.0	40.0	30.0	41.0
50	35.89452700315576	38.0	35.0	40.0	29.0	41.0
51	35.11785026104558	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	54.0
10	59.0
11	69.0
12	35.0
13	45.0
14	41.0
15	69.0
16	112.0
17	224.0
18	417.0
19	677.0
20	1231.0
21	1927.0
22	2883.0
23	4067.0
24	5600.0
25	7654.0
26	10054.0
27	13081.0
28	16393.0
29	21557.0
30	28398.0
31	36787.0
32	48130.0
33	65339.0
34	93840.0
35	106676.0
36	110526.0
37	140830.0
38	203114.0
39	251808.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.785169470552795	18.62119461421747	22.38045580145858	15.213180113771157
2	30.122646194912896	24.254710170281253	26.696369597534105	18.926274037271746
3	31.570301392868906	24.64743059123112	26.663258879731288	17.119009136168682
4	28.55662871450198	26.801846007854408	25.151259658003834	19.490265619639775
5	24.15256189912154	30.77001193009884	25.286347973163252	19.79107819761637
6	24.406097833637418	36.28806665836054	24.183539194385514	15.122296313616527
7	82.57360734443509	5.893451758196182	7.1244365631074915	4.40850433426123
8	83.77412687549175	4.461327876322936	6.697837392240828	5.066707855944483
9	77.90499613423836	7.192791421251961	9.369906453688518	5.532305990821163
10	36.428189850711625	29.806472974656646	17.850517056286513	15.914820118345215
11	27.63328343969156	27.98982101438268	25.42314302635374	18.953752519572017
12	24.58581734830939	24.405329801523436	30.725295393684725	20.28355745648245
13	23.120924096239545	25.795723938536952	31.699842809427338	19.383509155796165
14	21.07224110064122	27.932815964144847	29.90179429369207	21.093148641521864
15	19.522096283919286	27.1190432709293	33.36160829338144	19.997252151769974
16	22.175732574631386	26.390351468562738	31.447586928434767	19.986329028371106
17	22.388306796401515	25.649712499978666	28.896525593390145	23.065455110229678
18	23.608112467209295	25.472809103057966	30.085353968933955	20.833724460798788
19	22.922771810831982	27.969084147305146	27.84176149018711	21.26638255167576
20	23.97028227740296	27.063488948017877	28.222705418722576	20.743523355856585
21	23.61383003961339	28.199067096992213	28.387490975622658	19.799611887771736
22	21.896680906550973	25.57726147055962	28.100246964993097	24.425810657896307
23	18.043549127600855	35.6418103028948	25.294198968106187	21.02044160139816
24	21.20988952284725	26.248350864377475	30.40238055820574	22.139379054569535
25	20.5776284192363	28.19710434825648	27.922831546663073	23.302435685844145
26	20.509785582501156	27.924026263284823	29.198447551086936	22.367740603127086
27	17.679416568671456	39.80599508800795	23.413202984060774	19.101385359259822
28	20.538288107620073	27.419173153693464	29.452751517716795	22.589787220969665
29	21.10996001112793	25.887034423199353	30.030567678136517	22.972437887536206
30	22.076571095026054	25.81304732955234	29.32926902116867	22.781112554252935
31	22.492673827001617	26.96867965039178	26.671536559181995	23.867109963424603
32	20.931861897585478	29.517436889094455	27.864631779803485	21.68606943351658
33	21.244962989385797	25.494399339151038	28.23618864916805	25.02444902229512
34	21.178826890681723	25.232073703775132	30.236912306093227	23.352187099449917
35	20.503470651786188	24.36897628146158	30.845364414170707	24.282188652581528
36	21.612253013672678	25.960680169240142	29.541843242938796	22.88522357414838
37	21.158260697407293	25.782582055697688	29.879094677878797	23.18006256901622
38	20.66842688248938	26.662576184518862	29.9319182199405	22.737078713051257
39	21.21577776905445	24.900283830534566	29.357942220090692	24.525996180320288
40	21.797007405536316	24.1609249154738	30.98045272933012	23.061614949659763
41	18.88582434593532	25.22968427053163	29.3380587220287	26.546432661504355
42	20.756153217286524	24.84558287663868	29.504721690762963	24.89354221531183
43	21.41887959475212	24.168690573515182	29.92645665824107	24.485973173491626
44	21.57086461641916	25.06071720545542	29.09646995373033	24.27194822439509
45	21.031535398600134	24.36701353272585	29.514791445146294	25.086659623527723
46	21.561818904854473	24.858980770182605	29.199812941511794	24.379387383451125
47	20.033434998028717	25.134192277693106	30.88717949593199	23.945193228346188
48	20.169206008400565	24.560557625449512	30.488058807365597	24.782177558784323
49	21.778403960997622	23.33085287406151	30.318921068486276	24.571822096454593
50	20.52770633182742	23.655730458276228	30.910476470056135	24.906086739840216
51	20.027546751821514	23.24944146997933	29.04091563081891	27.682096147380243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2395.0
1	2772.5
2	3150.0
3	3921.5
4	4693.0
5	3402.0
6	2111.0
7	2168.0
8	2225.0
9	2315.0
10	2405.0
11	2533.5
12	2662.0
13	2611.0
14	2560.0
15	2687.0
16	2814.0
17	2976.0
18	3138.0
19	3057.0
20	2976.0
21	3510.5
22	4045.0
23	4698.0
24	5351.0
25	6674.5
26	9274.5
27	10551.0
28	12482.0
29	14413.0
30	16522.5
31	18632.0
32	21920.5
33	25209.0
34	29597.5
35	33986.0
36	37347.5
37	40709.0
38	43724.0
39	46739.0
40	51457.0
41	56175.0
42	63657.0
43	71139.0
44	77963.0
45	84787.0
46	95194.0
47	105601.0
48	115225.5
49	124850.0
50	121400.5
51	117951.0
52	104573.0
53	91195.0
54	79219.0
55	67243.0
56	59703.0
57	52163.0
58	47070.5
59	41978.0
60	37444.5
61	32911.0
62	30239.5
63	27568.0
64	23136.0
65	18704.0
66	15695.5
67	12687.0
68	10630.0
69	8573.0
70	7488.5
71	6404.0
72	5389.0
73	4374.0
74	3865.5
75	2925.0
76	2493.0
77	1960.5
78	1428.0
79	1206.0
80	984.0
81	618.0
82	252.0
83	212.5
84	173.0
85	103.0
86	33.0
87	20.0
88	7.0
89	19.5
90	32.0
91	16.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1171826.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.85593642680785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.90561159310916	24.682877512538486
2	11.28802118539664	7.643330552744976
3	4.449166476599107	4.518920921520721
4	2.334065677538283	3.1608791677892114
5	1.4297614072511389	2.420295565469894
6	0.9091852333108328	1.8468790475498411
7	0.6451840363481668	1.529031680273637
8	0.49497655493674975	1.340631582135917
9	0.39463255653896234	1.2024589271518586
>10	4.575866009783012	36.56966500517029
>50	0.5298794899324664	11.284254442224869
>100	0.0406220489116495	2.381229392174154
>500	0.0015138651719542545	0.353306240020912
>1k	0.0015138651719542545	1.066239963235257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	3021	0.2578027795935574	Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2614	0.22307066066122447	Illumina PCR Primer Index 10 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2046	0.1745993005787549	No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	1901	0.16222544985347653	Illumina PCR Primer Index 10 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1804	0.1539477704027731	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19576285216405848	0.0
2	0.0	0.0	0.0	0.9480076393594271	0.0
3	0.0	0.0	0.0	1.2862831171180704	0.0
4	0.0	0.0	0.0	1.643418050120069	0.0
5	0.0	0.0	0.0	2.7365837590222437	0.0
6	0.0	0.0	0.0	3.7230783409823642	0.0
7	0.0	0.0	0.0	4.5701324258038305	0.0
8	0.0	0.0	0.0	6.0394631967544665	0.0
9	0.0	0.0	0.0	6.672919016987164	0.0
10	0.0	0.0	0.0	7.993422231628245	0.0
11	0.0	0.0	0.0	9.440224060568719	0.0
12	0.0	0.0	0.0	10.644327741490631	0.0
13	0.0	0.0	0.0	11.110267223973525	0.0
14	0.0	0.0	0.0	11.331033788292801	0.0
15	0.0	0.0	0.0	11.634406473316004	0.0
16	0.0	0.0	0.0	12.298412904304906	0.0
17	0.0	0.0	0.0	13.021813818775142	0.0
18	0.0	0.0	0.0	13.925360932425122	0.0
19	0.0	0.0	0.0	14.356141611467914	0.0
20	0.0	0.0	0.0	14.780351349091077	0.0
21	0.0	0.0	0.0	15.310037497034543	0.0
22	0.0	0.0	0.0	15.845441217382103	0.0
23	8.533690155364363E-5	0.0	0.0	16.408579430734598	0.0
24	8.533690155364363E-5	0.0	0.0	16.84072550020225	0.0
25	8.533690155364363E-5	0.0	0.0	17.233360584250562	0.0
26	8.533690155364363E-5	0.0	0.0	17.589471474433918	0.0
27	8.533690155364363E-5	0.0	0.0	17.960857669995374	0.0
28	8.533690155364363E-5	0.0	0.0	18.414593975556098	0.0
29	8.533690155364363E-5	0.0	0.0	18.867306238298177	0.0
30	8.533690155364363E-5	0.0	0.0	19.39997917779602	0.0
31	1.7067380310728725E-4	0.0	0.0	19.871806906486118	0.0
32	1.7067380310728725E-4	0.0	0.0	20.354643095476632	0.0
33	1.7067380310728725E-4	0.0	0.0	20.826812171772943	0.0
34	1.7067380310728725E-4	0.0	0.0	21.32023013655611	0.0
35	1.7067380310728725E-4	0.0	0.0	21.78284147987841	0.0
36	1.7067380310728725E-4	0.0	0.0	22.245708833905375	0.0
37	1.7067380310728725E-4	0.0	0.0	22.722742113590243	0.0
38	1.7067380310728725E-4	0.0	0.0	23.199348708767342	0.0
39	1.7067380310728725E-4	0.0	0.0	23.67774737887707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGCG	35	1.2119563E-7	45.0	1
ATATGCG	35	1.2119563E-7	45.0	1
CCGTATG	20	7.033343E-4	45.0	1
TACGTCC	20	7.033343E-4	45.0	28
TTCTACG	30	2.1655396E-6	44.999996	1
ACGTCGT	30	2.1655396E-6	44.999996	26
TAGACGT	70	0.0	41.785713	26
CGTAGTC	65	0.0	41.538464	30
TTCGTAG	50	1.0822987E-9	40.500004	1
TAGTACG	45	1.9283107E-8	40.0	1
TTGGACG	80	0.0	39.375	1
TAACGCG	40	3.458299E-7	39.375	1
TCGACGT	40	3.458299E-7	39.375	26
TCGTAGG	130	0.0	38.076927	2
CTAACGC	255	0.0	37.941177	25
TAACGTT	30	1.1399357E-4	37.499996	38
TCGCTAA	30	1.1399357E-4	37.499996	19
CATCGAT	30	1.1399357E-4	37.499996	16
TTACGAC	30	1.1399357E-4	37.499996	14
TGTCGAC	30	1.1399357E-4	37.499996	12
>>END_MODULE