##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552694_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	533509
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81719896009252	31.0	31.0	34.0	30.0	34.0
2	31.928396709333864	33.0	31.0	34.0	30.0	34.0
3	31.9659274726387	33.0	31.0	34.0	30.0	34.0
4	35.63164445210859	37.0	35.0	37.0	33.0	37.0
5	35.523357619084216	37.0	35.0	37.0	33.0	37.0
6	35.46077573199327	37.0	35.0	37.0	33.0	37.0
7	35.768770536204634	37.0	35.0	37.0	35.0	37.0
8	35.56005053335558	37.0	35.0	37.0	35.0	37.0
9	37.375663765747156	39.0	37.0	39.0	34.0	39.0
10	36.93301143935716	39.0	37.0	39.0	33.0	39.0
11	36.82007051427436	39.0	37.0	39.0	32.0	39.0
12	36.69560213604644	39.0	35.0	39.0	32.0	39.0
13	36.550931661883865	39.0	35.0	39.0	32.0	39.0
14	37.67221546403154	40.0	36.0	41.0	33.0	41.0
15	37.8427168051523	40.0	36.0	41.0	33.0	41.0
16	37.888406756024736	40.0	36.0	41.0	33.0	41.0
17	37.843104802355725	40.0	36.0	41.0	33.0	41.0
18	37.83709365727663	40.0	36.0	41.0	33.0	41.0
19	37.77872913109245	40.0	36.0	41.0	33.0	41.0
20	37.679518058739404	40.0	36.0	41.0	33.0	41.0
21	37.511292218125654	39.0	35.0	41.0	32.0	41.0
22	37.559203312408975	39.0	35.0	41.0	33.0	41.0
23	33.512090705124	39.0	34.0	41.0	0.0	41.0
24	35.149264585976994	39.0	34.0	41.0	17.0	41.0
25	36.1972937663657	39.0	34.0	41.0	28.0	41.0
26	36.79470824297247	39.0	35.0	41.0	31.0	41.0
27	30.635689369813818	38.0	31.0	40.0	0.0	41.0
28	33.192195445625096	38.0	31.0	40.0	16.0	41.0
29	35.14703781941823	38.0	32.0	40.0	25.0	41.0
30	36.135894614711276	38.0	35.0	40.0	30.0	41.0
31	36.53388602629009	38.0	35.0	40.0	30.0	41.0
32	35.02848874152076	38.0	35.0	40.0	26.0	41.0
33	35.684541404175	38.0	35.0	40.0	26.0	41.0
34	36.132683797274275	38.0	35.0	40.0	29.0	41.0
35	36.31072765407894	38.0	35.0	40.0	30.0	41.0
36	36.34482642279699	38.0	35.0	40.0	30.0	41.0
37	36.36594884060063	39.0	35.0	40.0	30.0	41.0
38	36.29144962877852	38.0	35.0	40.0	30.0	41.0
39	36.24133238614532	38.0	35.0	40.0	30.0	41.0
40	36.11549570860098	38.0	35.0	40.0	30.0	41.0
41	36.04344256610479	38.0	35.0	40.0	30.0	41.0
42	35.990566232247254	38.0	35.0	40.0	30.0	41.0
43	35.97912687508552	38.0	35.0	40.0	30.0	41.0
44	35.88673293234041	38.0	35.0	40.0	30.0	41.0
45	35.809635826199745	38.0	35.0	40.0	29.0	41.0
46	35.66975252526199	38.0	34.0	40.0	29.0	41.0
47	35.5804663089095	38.0	34.0	40.0	29.0	41.0
48	35.52706702229953	37.0	34.0	40.0	29.0	41.0
49	35.496177196635855	37.0	34.0	40.0	29.0	41.0
50	35.380329104101335	37.0	34.0	40.0	28.0	41.0
51	34.676001716934486	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	31.0
10	28.0
11	42.0
12	37.0
13	28.0
14	39.0
15	41.0
16	77.0
17	133.0
18	263.0
19	410.0
20	750.0
21	1146.0
22	1704.0
23	2300.0
24	3195.0
25	4125.0
26	5369.0
27	6785.0
28	8217.0
29	10369.0
30	13354.0
31	17202.0
32	22110.0
33	29685.0
34	44229.0
35	51117.0
36	49534.0
37	62841.0
38	85644.0
39	112636.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.85624235017591	18.342520932167965	22.13402210646868	15.667214611187442
2	29.846356856210488	25.94482942180919	26.56243849682011	17.646375225160213
3	32.634875887754475	24.381031997585794	26.78736441184685	16.196727702812886
4	29.367077218941013	26.58999192141088	25.02938094765037	19.013549911997735
5	24.635760596353578	30.7709898052329	24.142048212869884	20.451201385543634
6	24.160792039122114	36.180458061625956	24.349917246007095	15.308832653244838
7	81.28747593761305	6.964830958802944	7.460042848386812	4.287650255197194
8	82.91875113634447	5.90636709033962	7.029872035898175	4.145009737417738
9	77.2828574588245	8.148878463156198	9.05270576503864	5.515558312980662
10	42.02215895139538	25.989252290026972	15.661216586786727	16.327372171790916
11	32.13272878245728	27.1974793302456	21.901036346153486	18.768755541143637
12	28.29005696248798	23.295014704531695	28.77064866759511	19.644279665385213
13	24.906046570910707	25.812872884993503	29.530710822122963	19.750369721972824
14	21.29448612863138	28.544410684730714	28.63756750120429	21.523535685433608
15	21.682483332052506	25.802751218817306	31.294504872457633	21.22026057667256
16	23.118447861235705	24.750097936492168	30.405110316789408	21.72634388548272
17	23.150874680652063	24.488246683748542	27.91911664095639	24.441761994643013
18	24.631636954578088	25.057309248766185	27.469452249165432	22.841601547490296
19	25.47304731504061	26.502833129338025	25.819245785919264	22.204873769702104
20	26.273783572535798	25.511847035382722	26.544819300143015	21.669550091938465
21	26.204056538877506	26.61004781550077	26.313895360715566	20.872000284906157
22	24.224146171854645	25.946328927909367	25.69684860049221	24.13267629974377
23	21.119606229698093	34.22885087224395	23.526688397009234	21.124854501048716
24	23.149562612814403	26.161133176759904	27.745736248123276	22.943567962302417
25	23.324817388272738	27.077143965706295	26.229735580843062	23.368303065177905
26	21.513226580994885	28.122674594055585	26.869274932569088	23.49482389238045
27	19.29227060836837	39.872616956789855	21.807692091417387	19.027420343424385
28	21.593825033879465	28.02876802453192	27.890438586790477	22.48696835479814
29	23.159309402465563	26.217739532041634	27.381918580567525	23.241032484925277
30	23.751801750298497	27.230843340974566	26.9425632932153	22.07479161551164
31	25.082988290731738	27.408159937320647	23.688635055828485	23.820216716119127
32	23.41291337165821	31.977155024563785	23.27477137217929	21.335160231598717
33	24.018526397867703	27.88706469806507	24.014590194354735	24.079818709712487
34	22.50664937236298	28.102243823440652	25.590945982167124	23.800160822029245
35	23.7703581382882	26.43666742266766	25.666483601963602	24.126490837080535
36	24.83650697551494	28.452378497832274	23.921245939618636	22.789868587034146
37	24.24270255984435	28.11836351401757	25.932833373007764	21.706100553130312
38	23.37092720085322	29.331276510799253	24.45300829039435	22.844787997953176
39	24.44213687116806	27.052589553315876	25.38626339949279	23.119010176023274
40	23.217602702109993	27.670760943114363	27.270767690891812	21.840868663883832
41	21.6000104965427	27.188857170169577	26.817541972112934	24.393590361174788
42	22.209747164527684	27.044904584552466	26.575184298671623	24.170163952248227
43	23.342624023212355	25.17408328631757	27.240777568888248	24.242515121581828
44	23.154436007639983	26.75962354899355	25.9633858097989	24.12255463356757
45	24.161729230434727	26.37331328993513	25.63836786258526	23.826589617044885
46	22.61105248458789	28.321921467116766	25.718029124157233	23.348996924138113
47	22.49952671838713	27.36017574211494	27.69025452241668	22.45004301708125
48	22.49202918788624	27.179672695305985	26.670590374295465	23.65770774251231
49	22.765126736381205	26.536197140066992	26.80910725029943	23.889568873252372
50	21.12597913062385	27.083141990107006	27.670760943114363	24.120117936154777
51	21.931588782944615	26.16263268286008	26.603300038049966	25.30247849614533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1346.0
1	1721.5
2	2097.0
3	2467.0
4	2837.0
5	2098.0
6	1359.0
7	1299.5
8	1240.0
9	1301.0
10	1362.0
11	1466.0
12	1570.0
13	1516.5
14	1463.0
15	1553.5
16	1644.0
17	1614.0
18	1584.0
19	1533.0
20	1482.0
21	1710.5
22	1939.0
23	2437.5
24	2936.0
25	3386.5
26	4731.0
27	5625.0
28	6673.0
29	7721.0
30	8644.5
31	9568.0
32	10188.0
33	10808.0
34	12608.0
35	14408.0
36	15474.0
37	16540.0
38	17250.5
39	17961.0
40	19402.5
41	20844.0
42	23080.5
43	25317.0
44	29216.5
45	33116.0
46	35641.0
47	38166.0
48	39882.0
49	41598.0
50	41254.5
51	40911.0
52	37653.5
53	34396.0
54	32633.0
55	30870.0
56	29436.0
57	28002.0
58	27776.0
59	27550.0
60	26258.0
61	24966.0
62	23053.0
63	21140.0
64	18898.0
65	16656.0
66	14571.5
67	12487.0
68	10869.5
69	9252.0
70	7998.5
71	6745.0
72	5660.5
73	4576.0
74	3800.0
75	2381.5
76	1739.0
77	1536.5
78	1334.0
79	1077.0
80	820.0
81	598.5
82	377.0
83	290.5
84	204.0
85	126.5
86	49.0
87	39.5
88	30.0
89	17.5
90	5.0
91	5.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	533509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.653156906364448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12798998662163	23.46384898199947
2	9.225046976994353	5.840037188627705
3	3.8047012701298026	3.6129241885578818
4	2.1178122610365295	2.681417751872469
5	1.2388931921711726	1.9607440301010421
6	0.8890946277343744	1.6885591053769102
7	0.6891567942241674	1.5269791698465283
8	0.5410228745448714	1.3700065550320912
9	0.4547342931195257	1.2954398337735362
>10	6.535087413157084	46.81005310563163
>50	0.3328639676065439	6.472394155291977
>100	0.040650643831595626	2.471437125160901
>500	0.0017674192970258968	0.39478098498193825
>1k	0.0011782795313505978	0.4113778237459403
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1114	0.20880622444982183	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1092	0.20468258267433165	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	832	0.1559486344185384	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	691	0.12951983940289669	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	594	0.11133832793823535	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7487652504456344E-4	0.0	0.0	0.07235116933360075	0.0
2	3.7487652504456344E-4	0.0	0.0	0.34938492134153315	0.0
3	3.7487652504456344E-4	0.0	0.0	0.4695328476183157	0.0
4	3.7487652504456344E-4	0.0	0.0	0.6114236123476829	0.0
5	3.7487652504456344E-4	0.0	0.0	1.0661488372267385	0.0
6	3.7487652504456344E-4	0.0	0.0	1.4481480162471485	0.0
7	3.7487652504456344E-4	0.0	0.0	1.7765398521861862	0.0
8	3.7487652504456344E-4	0.0	0.0	2.3435405963160885	0.0
9	3.7487652504456344E-4	0.0	0.0	2.6082034229975504	0.0
10	3.7487652504456344E-4	0.0	0.0	3.2211265414454116	0.0
11	3.7487652504456344E-4	0.0	0.0	3.953447833119966	0.0
12	3.7487652504456344E-4	0.0	0.0	4.5322571877887725	0.0
13	3.7487652504456344E-4	0.0	0.0	4.728505048649601	0.0
14	3.7487652504456344E-4	0.0	0.0	4.828409642573977	0.0
15	3.7487652504456344E-4	0.0	0.0	4.968238586415599	0.0
16	3.7487652504456344E-4	0.0	0.0	5.253707060237034	0.0
17	3.7487652504456344E-4	0.0	0.0	5.566728958649245	0.0
18	3.7487652504456344E-4	0.0	0.0	6.010395326039486	0.0
19	3.7487652504456344E-4	0.0	0.0	6.211891458250939	0.0
20	3.7487652504456344E-4	0.0	0.0	6.401953856448532	0.0
21	3.7487652504456344E-4	0.0	0.0	6.637188875913996	0.0
22	3.7487652504456344E-4	0.0	0.0	6.877297290205039	0.0
23	3.7487652504456344E-4	0.0	0.0	7.132775642022909	0.0
24	3.7487652504456344E-4	0.0	0.0	7.333709459446795	0.0
25	3.7487652504456344E-4	0.0	0.0	7.527145746369789	0.0
26	3.7487652504456344E-4	0.0	0.0	7.711584996691714	0.0
27	3.7487652504456344E-4	0.0	0.0	7.895836808751118	0.0
28	3.7487652504456344E-4	0.0	0.0	8.112515440226876	0.0
29	3.7487652504456344E-4	0.0	0.0	8.344938885754505	0.0
30	3.7487652504456344E-4	0.0	0.0	8.590857886183738	0.0
31	3.7487652504456344E-4	0.0	0.0	8.836214571825405	0.0
32	3.7487652504456344E-4	0.0	0.0	9.08888134970544	0.0
33	3.7487652504456344E-4	0.0	0.0	9.356730626849782	0.0
34	3.7487652504456344E-4	0.0	0.0	9.6243924657316	0.0
35	3.7487652504456344E-4	0.0	0.0	9.923356494454639	0.0
36	3.7487652504456344E-4	0.0	0.0	10.200390246462572	0.0
37	3.7487652504456344E-4	0.0	0.0	10.465240511406556	0.0
38	3.7487652504456344E-4	0.0	0.0	10.748647164340245	0.0
39	3.7487652504456344E-4	0.0	0.0	11.009186349246217	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAT	20	7.0298824E-4	45.000004	32
CGAAATT	20	7.0298824E-4	45.000004	44
GATCAGC	20	7.0298824E-4	45.000004	9
TAACGCA	35	1.2102828E-7	45.000004	26
GTTAACG	20	7.0298824E-4	45.000004	1
AATTGCG	35	1.2102828E-7	45.000004	1
GTATGCG	20	7.0298824E-4	45.000004	1
CCGAAAT	20	7.0298824E-4	45.000004	43
TTCGCAT	20	7.0298824E-4	45.000004	25
ATTCTCG	20	7.0298824E-4	45.000004	30
CTCGCGG	25	3.8879858E-5	45.0	2
ATAGTAG	30	2.1633077E-6	44.999996	1
CCCGAAT	30	2.1633077E-6	44.999996	40
GCTACGA	135	0.0	43.333332	10
ACGGGCT	70	0.0	41.785717	5
GGCCGAT	55	6.002665E-11	40.909092	8
CGAGCTA	50	1.0786607E-9	40.5	17
CGTATGG	45	1.9250365E-8	40.000004	2
AATGCGG	90	0.0	40.000004	2
ACGGGAT	175	0.0	39.857143	5
>>END_MODULE