FastQCFastQC Report
Sat 18 Jun 2016
SRR3552693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences729673
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36070.4943310222524336No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC35050.4803521577473745No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC34110.467469674772124No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG30640.41991412591667776No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC18720.2565532779752026TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17780.24367079499995203No Hit
GAACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT12290.16843161251683972No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCGCTGT10400.14252959887511255No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9160.12553568516307992No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470.11607939446848109No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT8010.10977520067208188No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC7910.10840472375982119TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGAA253.8893995E-545.00000428
TCCGACC253.8893995E-545.00000428
AATAGCG253.8893995E-545.0000041
TCGAACA253.8893995E-545.00000430
CCCGACA253.8893995E-545.00000423
GTCGATG207.0315925E-445.01
CTATCGA207.0315925E-445.041
CGACCGT207.0315925E-445.015
TCGATAG207.0315925E-445.01
GTTAACG351.2111195E-745.01
GTTCGCC207.0315925E-445.029
CGCTACA850.045.028
CTTGACG207.0315925E-445.01
ACGTCGA207.0315925E-445.030
ACGTCCA207.0315925E-445.045
CTCGACG351.2111195E-745.01
GTATACG207.0315925E-445.01
CGGTCGA406.8084773E-945.041
TGTACCG207.0315925E-445.019
TACGTCG207.0315925E-445.029