##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552693_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	729673
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.709698728060378	31.0	31.0	34.0	30.0	34.0
2	31.83356791329815	31.0	31.0	34.0	30.0	34.0
3	31.857301832464678	33.0	31.0	34.0	30.0	34.0
4	35.551244187464796	37.0	35.0	37.0	33.0	37.0
5	35.435903480052026	37.0	35.0	37.0	33.0	37.0
6	35.37915203111531	37.0	35.0	37.0	33.0	37.0
7	35.73838280983399	37.0	35.0	37.0	35.0	37.0
8	35.43912958270349	37.0	35.0	37.0	33.0	37.0
9	37.32483317869786	39.0	37.0	39.0	34.0	39.0
10	36.77618193355106	39.0	37.0	39.0	32.0	39.0
11	36.69911179391316	39.0	35.0	39.0	32.0	39.0
12	36.687250316237545	39.0	35.0	39.0	32.0	39.0
13	36.55061102713133	39.0	35.0	39.0	32.0	39.0
14	37.69340923948125	40.0	36.0	41.0	32.0	41.0
15	37.833293817915695	40.0	37.0	41.0	33.0	41.0
16	37.91045714998362	40.0	37.0	41.0	33.0	41.0
17	37.77338204921931	40.0	36.0	41.0	33.0	41.0
18	37.76615826541478	39.0	36.0	41.0	33.0	41.0
19	37.67192701388156	39.0	36.0	41.0	33.0	41.0
20	37.426867925769486	39.0	35.0	41.0	32.0	41.0
21	37.3515081413181	39.0	35.0	41.0	32.0	41.0
22	37.422592037803234	39.0	35.0	41.0	32.0	41.0
23	33.54773713704632	39.0	34.0	41.0	8.0	41.0
24	35.15817633378239	39.0	34.0	41.0	18.0	41.0
25	36.1721716440104	38.0	34.0	41.0	29.0	41.0
26	36.67559303962186	38.0	35.0	40.0	30.0	41.0
27	30.803187181107152	38.0	31.0	40.0	0.0	41.0
28	33.26469665178786	38.0	31.0	40.0	16.0	41.0
29	35.03028342833022	38.0	32.0	40.0	25.0	41.0
30	35.967186671289745	38.0	34.0	40.0	30.0	41.0
31	36.33666587635832	38.0	35.0	40.0	30.0	41.0
32	34.74890259061251	38.0	35.0	40.0	23.0	41.0
33	35.30374427997199	38.0	35.0	40.0	23.0	41.0
34	35.6354915146922	38.0	35.0	40.0	26.0	41.0
35	35.70561196590802	38.0	35.0	40.0	27.0	41.0
36	35.771773109324315	39.0	35.0	40.0	27.0	41.0
37	35.72954049279609	39.0	35.0	40.0	27.0	41.0
38	35.70016569065869	39.0	35.0	40.0	27.0	41.0
39	35.63258336268438	39.0	35.0	40.0	27.0	41.0
40	35.582959764168336	38.0	35.0	40.0	26.0	41.0
41	35.56040856657708	38.0	35.0	40.0	26.0	41.0
42	35.36986430908092	38.0	35.0	40.0	26.0	41.0
43	35.32662302154527	38.0	35.0	40.0	26.0	41.0
44	35.35216459975907	38.0	35.0	40.0	26.0	41.0
45	35.29266397413636	38.0	35.0	40.0	26.0	41.0
46	35.15356330849573	38.0	34.0	40.0	25.0	41.0
47	35.03050955702075	38.0	34.0	40.0	25.0	41.0
48	35.02480700258883	38.0	34.0	40.0	25.0	41.0
49	35.00803373565967	38.0	34.0	40.0	25.0	41.0
50	34.854148365089564	38.0	34.0	40.0	24.0	41.0
51	34.22374817212642	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	41.0
10	49.0
11	43.0
12	42.0
13	29.0
14	38.0
15	53.0
16	93.0
17	204.0
18	446.0
19	912.0
20	1883.0
21	3353.0
22	4947.0
23	6238.0
24	7393.0
25	8545.0
26	9876.0
27	10884.0
28	11872.0
29	13992.0
30	17531.0
31	22539.0
32	29094.0
33	37915.0
34	54498.0
35	63694.0
36	65031.0
37	81244.0
38	117238.0
39	159858.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.063510641067985	17.799205945677034	18.157311562850754	17.979971850404223
2	29.35438203140311	29.020807950958854	24.43724791790295	17.18756209973509
3	28.984627360475173	28.696690161209197	27.21780852518868	15.100873953126948
4	25.447974640695215	26.623432688341214	30.838882622763897	17.08971004819967
5	26.879437775551512	29.462923802854156	25.876248675776687	17.78138974581765
6	23.126249703634368	37.857368985833375	25.46291283903886	13.553468471493396
7	77.82842451344644	5.618818292577634	13.09367346742993	3.459083726546001
8	78.56395947225676	8.67114447156466	8.791061201387471	3.973834854791119
9	73.29543507845295	7.498016234669502	10.578163094975421	8.628385591902125
10	37.97399657106676	28.394088858982037	19.483796166227883	14.148118403723311
11	29.289558473453177	26.40854190849874	25.949569190582633	18.352330427465454
12	22.625340392203082	23.39500022612869	31.959247498537014	22.02041188313121
13	24.538800257101467	25.4268692962464	32.75152020151493	17.282810245137206
14	19.92303401660744	30.5129832130283	29.721669843888975	19.842312926475284
15	19.42993642357604	25.800872445602348	33.68522612183814	21.083965008983476
16	20.98583886206561	25.02929394399957	31.357471086363343	22.627396107571474
17	20.795205523570147	27.16381173484561	30.5287436975193	21.512239044064945
18	22.073723435018152	24.00335492748121	31.57839196461977	22.344529672880867
19	21.63544491847718	27.01155174989345	29.08398693661407	22.2690163950153
20	24.904717592675073	25.63257788077673	30.91398475755578	18.54871976899241
21	21.642982541494614	29.694123257952537	29.95903644509253	18.703857755460323
22	20.86414051225686	24.48781851596537	31.670076870050007	22.97796410172776
23	18.79800951933263	35.21138921133165	27.05814796491031	18.932453304425405
24	20.062685613966806	28.074356595351617	30.91850733136624	20.944450459315338
25	19.070734424872512	30.9550990649236	28.920763136363824	21.053403373840062
26	19.37689896707155	27.861521530877532	31.31923478051127	21.442344721539648
27	18.617791805370352	39.88115224216875	24.52262862953679	16.978427322924105
28	19.06511546953224	29.063018639856487	31.469576097786266	20.402289792825005
29	20.467798589231066	26.74554218122364	31.213296915193517	21.573362314351773
30	20.363642343899254	29.79499035869492	30.156111025075617	19.68525627233021
31	21.308586174903006	27.214245285216805	28.09957337053721	23.377595169342978
32	21.874867235049127	31.011425666017516	27.022104422117856	20.091602676815505
33	23.021819362920102	27.30181875991026	26.91274036451945	22.763621512650186
34	20.09914029983294	27.744482802570463	28.46453137227224	23.691845525324357
35	21.79099404801877	26.868199864870977	28.630633174038234	22.710172913072018
36	21.72781506236355	29.94382415136643	27.699257064465865	20.62910372180415
37	20.03034235883745	28.07833097839717	28.94365010079858	22.947676561966798
38	21.170442102147128	27.129138668965414	28.243610494015815	23.456808734871647
39	22.288066024095727	26.46706127265227	27.602912537533935	23.641960165718068
40	22.50473842392414	25.348203921482636	29.197462424949254	22.94959522964396
41	20.428054758775506	26.27875774490765	28.468094612244116	24.825092884072728
42	21.861573609000196	26.360164073495934	29.744008617558826	22.034253699945044
43	22.762662178811606	26.56367989496665	27.959510630104166	22.714147296117577
44	21.000228869644346	26.947139335017194	29.008336611057285	23.04429518428118
45	21.279943207436755	26.230105814522396	28.493996625885842	23.995954352155007
46	22.89738005928683	27.629088646558113	27.19930708687316	22.274224207281893
47	20.166293668533715	28.138631962536643	29.97287826190636	21.722196107023283
48	20.675425841438564	27.31716810132758	29.402485771023457	22.604920286210398
49	21.538004010015445	25.233906147000095	30.767617823326336	22.46047201965812
50	21.428639952417043	26.14239529213771	29.44346303070005	22.985501724745195
51	19.62975195738365	25.765925284339698	28.74972761771369	25.854595140562964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2007.0
1	2291.0
2	2575.0
3	5074.5
4	7574.0
5	5258.5
6	2943.0
7	2892.0
8	2841.0
9	2920.5
10	3000.0
11	3138.5
12	3277.0
13	3223.5
14	3170.0
15	3000.5
16	2831.0
17	2792.0
18	2753.0
19	2930.0
20	3107.0
21	3232.0
22	3357.0
23	3702.5
24	4048.0
25	4838.0
26	6681.0
27	7734.0
28	9167.0
29	10600.0
30	12971.0
31	15342.0
32	16110.5
33	16879.0
34	19922.5
35	22966.0
36	24563.5
37	26161.0
38	29037.5
39	31914.0
40	33680.0
41	35446.0
42	40118.0
43	44790.0
44	48131.0
45	51472.0
46	60316.0
47	69160.0
48	66408.5
49	63657.0
50	63362.5
51	63068.0
52	57126.0
53	51184.0
54	46289.5
55	41395.0
56	37361.0
57	33327.0
58	29585.0
59	25843.0
60	23060.5
61	20278.0
62	18077.5
63	15877.0
64	13676.5
65	11476.0
66	9181.0
67	6886.0
68	5735.0
69	4584.0
70	4170.5
71	3757.0
72	3198.5
73	2640.0
74	2099.5
75	1376.5
76	1194.0
77	940.0
78	686.0
79	489.5
80	293.0
81	247.5
82	202.0
83	136.5
84	71.0
85	90.5
86	110.0
87	58.0
88	6.0
89	4.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	729673.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.71857118977137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16357841879253	25.344084697920184
2	9.518590773748796	6.419065612618994
3	3.8678672018439184	3.9125686679386784
4	1.9776157978969644	2.667295162696212
5	1.2438003253569108	2.096958490820389
6	0.885766741644513	1.79200733614034
7	0.6592058317395944	1.555923513635677
8	0.5037283944315727	1.358800138236017
9	0.427680952801965	1.2978711588206076
>10	5.374129866404509	40.791144118929545
>50	0.33530122549755037	7.014078185915185
>100	0.03615993601946722	2.2167003146435054
>500	0.003287266910860657	0.8308989178646854
>1k	0.003287266910860657	2.7026036838200023
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3607	0.4943310222524336	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGC	3505	0.4803521577473745	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTC	3411	0.467469674772124	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCG	3064	0.41991412591667776	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	1872	0.2565532779752026	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1778	0.24367079499995203	No Hit
GAACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCT	1229	0.16843161251683972	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATCGCTGT	1040	0.14252959887511255	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	916	0.12553568516307992	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	847	0.11607939446848109	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCT	801	0.10977520067208188	No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	791	0.10840472375982119	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7409538245213954E-4	0.0	0.0	0.11950558674913284	0.0
2	2.7409538245213954E-4	0.0	0.0	0.61685165820854	0.0
3	2.7409538245213954E-4	0.0	0.0	0.8006326121426995	0.0
4	2.7409538245213954E-4	0.0	0.0	1.2032787289648925	0.0
5	2.7409538245213954E-4	0.0	0.0	2.2542974729776213	0.0
6	2.7409538245213954E-4	0.0	0.0	2.7221782908234236	0.0
7	2.7409538245213954E-4	0.0	0.0	3.2467968528368187	0.0
8	2.7409538245213954E-4	0.0	0.0	4.06305290177929	0.0
9	2.7409538245213954E-4	0.0	0.0	4.37689211468699	0.0
10	2.7409538245213954E-4	0.0	0.0	5.59839818658495	0.0
11	2.7409538245213954E-4	0.0	0.0	6.468651025870493	0.0
12	2.7409538245213954E-4	0.0	0.0	7.746620746553593	0.0
13	2.7409538245213954E-4	0.0	0.0	8.088280640780185	0.0
14	2.7409538245213954E-4	0.0	0.0	8.248352344132234	0.0
15	2.7409538245213954E-4	0.0	0.0	8.556709649390891	0.0
16	2.7409538245213954E-4	0.0	0.0	8.99019149673895	0.0
17	2.7409538245213954E-4	0.0	0.0	9.451631073097127	0.0
18	2.7409538245213954E-4	0.0	0.0	10.016130513257309	0.0
19	2.7409538245213954E-4	0.0	0.0	10.487026380310084	0.0
20	2.7409538245213954E-4	0.0	0.0	10.79223158867054	0.0
21	2.7409538245213954E-4	0.0	0.0	11.112923186139545	0.0
22	2.7409538245213954E-4	0.0	0.0	11.476921854035986	0.0
23	2.7409538245213954E-4	0.0	0.0	11.840235283476297	0.0
24	2.7409538245213954E-4	0.0	0.0	12.121320098180966	0.0
25	2.7409538245213954E-4	0.0	0.0	12.378695662303524	0.0
26	2.7409538245213954E-4	0.0	0.0	12.614006548138686	0.0
27	2.7409538245213954E-4	0.0	0.0	12.878508592205002	0.0
28	2.7409538245213954E-4	0.0	0.0	13.181658085197068	0.0
29	2.7409538245213954E-4	0.0	0.0	13.486178055101394	0.0
30	2.7409538245213954E-4	0.0	0.0	13.834553286198064	0.0
31	2.7409538245213954E-4	0.0	0.0	14.15099640523906	0.0
32	2.7409538245213954E-4	0.0	0.0	14.466480190441471	0.0
33	2.7409538245213954E-4	0.0	0.0	14.793201886324422	0.0
34	2.7409538245213954E-4	0.0	0.0	15.119512439133693	0.0
35	2.7409538245213954E-4	0.0	0.0	15.469121099451398	0.0
36	2.7409538245213954E-4	0.0	0.0	15.802146989130748	0.0
37	2.7409538245213954E-4	0.0	0.0	16.15216679252213	0.0
38	2.7409538245213954E-4	0.0	0.0	16.49917154670654	0.0
39	2.7409538245213954E-4	0.0	0.0	16.83672001019635	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGAA	25	3.8893995E-5	45.000004	28
TCCGACC	25	3.8893995E-5	45.000004	28
AATAGCG	25	3.8893995E-5	45.000004	1
TCGAACA	25	3.8893995E-5	45.000004	30
CCCGACA	25	3.8893995E-5	45.000004	23
GTCGATG	20	7.0315925E-4	45.0	1
CTATCGA	20	7.0315925E-4	45.0	41
CGACCGT	20	7.0315925E-4	45.0	15
TCGATAG	20	7.0315925E-4	45.0	1
GTTAACG	35	1.2111195E-7	45.0	1
GTTCGCC	20	7.0315925E-4	45.0	29
CGCTACA	85	0.0	45.0	28
CTTGACG	20	7.0315925E-4	45.0	1
ACGTCGA	20	7.0315925E-4	45.0	30
ACGTCCA	20	7.0315925E-4	45.0	45
CTCGACG	35	1.2111195E-7	45.0	1
GTATACG	20	7.0315925E-4	45.0	1
CGGTCGA	40	6.8084773E-9	45.0	41
TGTACCG	20	7.0315925E-4	45.0	19
TACGTCG	20	7.0315925E-4	45.0	29
>>END_MODULE