##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552689_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593497
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.875354045597536	33.0	31.0	34.0	30.0	34.0
2	32.00540019578869	33.0	31.0	34.0	30.0	34.0
3	32.03192433997139	33.0	31.0	34.0	30.0	34.0
4	35.681198051548705	37.0	35.0	37.0	33.0	37.0
5	35.580501670606594	37.0	35.0	37.0	33.0	37.0
6	35.542942929787344	37.0	35.0	37.0	33.0	37.0
7	35.88894636367159	37.0	35.0	37.0	35.0	37.0
8	35.710814039498096	37.0	35.0	37.0	35.0	37.0
9	37.545032241106526	39.0	37.0	39.0	35.0	39.0
10	37.04658995748925	39.0	37.0	39.0	33.0	39.0
11	36.894675120514506	39.0	37.0	39.0	33.0	39.0
12	36.811116147175134	39.0	35.0	39.0	33.0	39.0
13	36.69509028689277	39.0	35.0	39.0	33.0	39.0
14	37.85432108334162	40.0	37.0	41.0	33.0	41.0
15	38.015016082642376	40.0	37.0	41.0	33.0	41.0
16	38.07589254874077	40.0	37.0	41.0	33.0	41.0
17	38.02779795011601	40.0	37.0	41.0	33.0	41.0
18	37.97954328328534	40.0	37.0	41.0	33.0	41.0
19	37.89678128111852	40.0	36.0	41.0	33.0	41.0
20	37.79080433431003	40.0	36.0	41.0	33.0	41.0
21	37.655469193610074	40.0	35.0	41.0	33.0	41.0
22	37.707567182310946	39.0	35.0	41.0	33.0	41.0
23	33.59822206346452	39.0	35.0	41.0	0.0	41.0
24	35.23843759951609	39.0	35.0	41.0	18.0	41.0
25	36.35461341843345	39.0	35.0	41.0	29.0	41.0
26	36.98111026677473	39.0	35.0	41.0	31.0	41.0
27	30.7237290163219	38.0	32.0	41.0	0.0	41.0
28	33.318783414237984	38.0	32.0	40.0	16.0	41.0
29	35.31259130206218	38.0	32.0	40.0	25.0	41.0
30	36.33398989379896	38.0	35.0	40.0	30.0	41.0
31	36.7288478290539	39.0	35.0	40.0	31.0	41.0
32	35.15234954852341	39.0	35.0	40.0	26.0	41.0
33	35.79933344229204	39.0	35.0	41.0	26.0	41.0
34	36.23767264198471	39.0	35.0	41.0	30.0	41.0
35	36.4184957969459	39.0	35.0	41.0	30.0	41.0
36	36.462115225519256	39.0	35.0	41.0	30.0	41.0
37	36.47255672732971	39.0	35.0	41.0	30.0	41.0
38	36.45620449640015	39.0	35.0	41.0	30.0	41.0
39	36.41419417452826	39.0	35.0	41.0	30.0	41.0
40	36.308670473481754	39.0	35.0	41.0	30.0	41.0
41	36.204113921384604	39.0	35.0	41.0	30.0	41.0
42	36.145417752743484	39.0	35.0	40.0	30.0	41.0
43	36.14339078377818	39.0	35.0	41.0	30.0	41.0
44	36.057119075580836	39.0	35.0	40.0	30.0	41.0
45	35.99229650697476	38.0	35.0	40.0	30.0	41.0
46	35.920830265359385	38.0	35.0	40.0	29.0	41.0
47	35.83963187682499	38.0	35.0	40.0	29.0	41.0
48	35.81767894361724	38.0	35.0	40.0	29.0	41.0
49	35.785832110356075	38.0	35.0	40.0	29.0	41.0
50	35.67939686300015	38.0	35.0	40.0	29.0	41.0
51	34.985022670712745	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	16.0
10	30.0
11	36.0
12	27.0
13	32.0
14	30.0
15	44.0
16	82.0
17	137.0
18	258.0
19	496.0
20	875.0
21	1349.0
22	2020.0
23	2806.0
24	3722.0
25	4916.0
26	6371.0
27	7490.0
28	8814.0
29	10391.0
30	13834.0
31	17371.0
32	22374.0
33	30498.0
34	46782.0
35	54931.0
36	51782.0
37	65293.0
38	96836.0
39	143771.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.4457023371643	18.958309814539923	23.75142587072892	15.844561977566862
2	30.03098583480624	25.77873182172783	26.4843798704964	17.705902472969534
3	32.188031279012364	24.522280651797736	28.41631886934559	14.873369199844314
4	27.712187256211912	25.76221952259236	27.8225500718622	18.703043149333524
5	25.23365745740922	29.793242425825234	25.67039092025739	19.302709196508154
6	23.306099272616372	36.661853387632966	25.471737852086868	14.560309487663798
7	81.92728859623554	6.117806829689114	8.409983538248719	3.544921035826634
8	83.71836757388833	5.599691321101876	7.296919782239843	3.3850213227699553
9	77.95001491161707	8.31579603603725	9.153373985041204	4.580815067304468
10	37.831193754981065	33.69098748603616	15.624173331962925	12.853645427019849
11	28.780768900264032	25.792548235290152	28.63097875810661	16.79570410633921
12	25.88943162307476	24.732391233654088	30.66418195879676	18.713995184474395
13	22.426229618683834	27.55885876423975	31.648011700143385	18.366899916933026
14	19.347528294161553	30.50731511700986	30.115232258966767	20.02992432986182
15	18.382569751826882	28.565940518654685	34.34103289485878	18.710456834659652
16	21.382079437638268	26.098194262144542	33.12485151567742	19.39487478453977
17	22.400618705739035	25.60333076662561	29.82087525294989	22.175175274685465
18	22.795734435051905	24.832475985556794	30.826946050274895	21.544843529116406
19	22.872735666734627	27.232825102738513	29.29955838024455	20.59488085028231
20	24.992544191461793	25.823887905077868	29.703267244821795	19.480300658638544
21	23.991359686738097	27.23080318855866	29.588186629418516	19.189650495284727
22	21.880144297275304	26.121614768061168	29.371167840781	22.62707309388253
23	18.354094460460626	36.916951559991034	26.13138735326379	18.597566626284546
24	20.31198135795126	26.505946955081495	32.0498671433891	21.132204543578148
25	20.370279883470346	29.93999969671287	29.114384739939712	20.575335679877067
26	19.873899952316524	29.723149400923678	29.31455424374512	21.088396403014677
27	17.266473124548227	41.17476583706405	24.65926533748275	16.899495700904975
28	19.077265765454584	28.928874113938235	31.3871847709424	20.606675349664783
29	20.405663381617767	27.381604287806006	31.50041196501415	20.712320365562086
30	20.888226899209265	27.200137490164227	31.453065474635928	20.458570135990577
31	23.098347590636514	28.398121641726913	27.725666684077595	20.777864083558974
32	22.18073553868006	31.528381777835442	27.243103166486097	19.0477795169984
33	22.384780377996854	28.45288181743126	27.879332161746394	21.28300564282549
34	20.284011545129967	27.916232095528702	29.66956867515758	22.130187684183745
35	21.12765523667348	27.53678620110969	29.068217699499744	22.267340862717084
36	22.466667902280886	28.60014456686386	27.66096543032231	21.272222100532943
37	21.08148819623351	29.304444672845857	28.858949581884996	20.75511754903563
38	20.380052468672968	29.74151512139067	28.40149149869334	21.476940911243023
39	21.800615672867764	27.032992584629746	28.3411710589944	22.82522068350809
40	22.585960838892195	26.644616569249717	30.020707771058657	20.748714820799428
41	20.193867871278204	27.28050857881337	29.314385750896804	23.211237799011624
42	21.65571182331166	27.334089304579468	28.95532749112464	22.054871380984235
43	22.971472475850764	27.18210875539388	28.229628793405865	21.616789975349494
44	21.510134002362268	28.13999059809906	28.67377594158016	21.676099457958507
45	21.555458578560632	28.52802962778245	27.546558786312314	22.369953007344602
46	21.87475252612903	28.556504919148708	28.006038783683827	21.56270377103844
47	20.93388846110427	27.763072096404866	30.450701519973983	20.852337922516877
48	20.87036665728723	26.77115469833883	29.393240403911058	22.965238240462885
49	21.039027998456604	27.291797599650884	29.895180599059472	21.77399380283304
50	20.28300058804004	28.10258518577179	29.84159987329338	21.77281435289479
51	20.475587913671003	27.536280722564733	28.545384391159516	23.44274697260475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1875.0
1	2296.0
2	2717.0
3	4077.0
4	5437.0
5	3816.5
6	2196.0
7	2189.5
8	2183.0
9	2161.5
10	2140.0
11	2081.5
12	2023.0
13	2095.0
14	2167.0
15	2200.5
16	2234.0
17	2334.5
18	2435.0
19	2306.0
20	2177.0
21	2469.5
22	2762.0
23	2984.0
24	3206.0
25	4488.0
26	6837.5
27	7905.0
28	9642.5
29	11380.0
30	12459.5
31	13539.0
32	14280.5
33	15022.0
34	17818.0
35	20614.0
36	22368.0
37	24122.0
38	26076.0
39	28030.0
40	30314.5
41	32599.0
42	35726.5
43	38854.0
44	40808.5
45	42763.0
46	45483.0
47	48203.0
48	47668.5
49	47134.0
50	46200.0
51	45266.0
52	41968.0
53	38670.0
54	35604.5
55	32539.0
56	29153.0
57	25767.0
58	23441.0
59	21115.0
60	19786.5
61	18458.0
62	16578.5
63	14699.0
64	12342.5
65	9986.0
66	8070.5
67	6155.0
68	4967.0
69	3779.0
70	3173.5
71	2568.0
72	2212.0
73	1856.0
74	1481.5
75	1002.5
76	898.0
77	739.5
78	581.0
79	441.0
80	301.0
81	245.0
82	189.0
83	123.5
84	58.0
85	36.5
86	15.0
87	8.0
88	1.0
89	0.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	593497.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.31002746684874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83027204094978	20.436167847901018
2	8.816681715320412	4.81567639623727
3	3.815244360951114	3.1258328487094413
4	2.1507776569335975	2.3495118754376447
5	1.4089235786279608	1.9238870815510214
6	0.9533982369651703	1.562239922301838
7	0.7411818619108143	1.4169187904700096
8	0.5654763124995663	1.2354538900972398
9	0.42226283331894926	1.0378808618552888
>10	4.935862262856229	33.287060966968376
>50	1.2177093971299868	22.352926084128413
>100	0.1385159829900137	5.263408628600728
>500	0.0018468797732001825	0.34667950527613567
>1k	0.0018468797732001825	0.8463553004655998
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2335	0.39343080082965876	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1641	0.27649676409484797	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1058	0.1782654335236741	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	736	0.12401073636429502	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	668	0.1125532226784634	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	658	0.11086829419525288	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08727929543030546	0.0
2	0.0	0.0	0.0	0.3964636720994377	0.0
3	0.0	0.0	0.0	0.5196319442221274	0.0
4	0.0	0.0	0.0	0.6756563217674226	0.0
5	0.0	0.0	0.0	1.1477732827630132	0.0
6	0.0	0.0	0.0	1.4930151289728508	0.0
7	0.0	0.0	0.0	1.8111296266029988	0.0
8	0.0	0.0	0.0	2.3491272912921213	0.0
9	0.0	0.0	0.0	2.587881657363053	0.0
10	0.0	0.0	0.0	3.1629477486828073	0.0
11	0.0	0.0	0.0	3.822934235556372	0.0
12	0.0	0.0	0.0	4.401033198145905	0.0
13	0.0	0.0	0.0	4.578119181731331	0.0
14	0.0	0.0	0.0	4.675171062364258	0.0
15	0.0	0.0	0.0	4.814009169380806	0.0
16	0.0	0.0	0.0	5.106681246914475	0.0
17	0.0	0.0	0.0	5.416034116431928	0.0
18	0.0	0.0	0.0	5.848892243768713	0.0
19	1.68492848321053E-4	0.0	0.0	6.060856246956598	0.0
20	1.68492848321053E-4	0.0	0.0	6.263047664941862	0.0
21	1.68492848321053E-4	0.0	0.0	6.47888700364113	0.0
22	1.68492848321053E-4	0.0	0.0	6.715450962683889	0.0
23	1.68492848321053E-4	0.0	0.0	6.972065570676853	0.0
24	1.68492848321053E-4	0.0	0.0	7.160609067948111	0.0
25	1.68492848321053E-4	0.0	0.0	7.328427944875879	0.0
26	1.68492848321053E-4	0.0	0.0	7.48849615078088	0.0
27	1.68492848321053E-4	0.0	0.0	7.663728713034775	0.0
28	1.68492848321053E-4	0.0	0.0	7.877040659009229	0.0
29	1.68492848321053E-4	0.0	0.0	8.109560789692281	0.0
30	1.68492848321053E-4	0.0	0.0	8.382519203972388	0.0
31	1.68492848321053E-4	0.0	0.0	8.615544813200403	0.0
32	1.68492848321053E-4	0.0	0.0	8.839640301467405	0.0
33	1.68492848321053E-4	0.0	0.0	9.082606988746363	0.0
34	1.68492848321053E-4	0.0	0.0	9.316475062215984	0.0
35	1.68492848321053E-4	0.0	0.0	9.604597832844984	0.0
36	1.68492848321053E-4	0.0	0.0	9.858516555264812	0.0
37	1.68492848321053E-4	0.0	0.0	10.124735255612075	0.0
38	1.68492848321053E-4	0.0	0.0	10.386910127599634	0.0
39	3.36985696642106E-4	0.0	0.0	10.652454856553614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGTCG	35	1.210592E-7	45.000004	1
TAAGACG	35	1.210592E-7	45.000004	1
TAGTCGG	35	1.210592E-7	45.000004	2
TCGCCCG	25	3.8885177E-5	45.0	1
GCGACGT	20	7.0305256E-4	45.0	41
GGTAACG	25	3.8885177E-5	45.0	33
CAACGTA	20	7.0305256E-4	45.0	33
GGTCGCG	20	7.0305256E-4	45.0	28
TCCGATA	40	6.8048394E-9	45.0	17
TCCGAGC	25	3.8885177E-5	45.0	12
CGGCCGA	20	7.0305256E-4	45.0	39
TAAACCG	20	7.0305256E-4	45.0	1
TAACGGA	20	7.0305256E-4	45.0	3
ACGGGTC	40	6.8048394E-9	45.0	5
CCGTCGA	25	3.8885177E-5	45.0	41
ATCCGCG	20	7.0305256E-4	45.0	1
TCGGATT	20	7.0305256E-4	45.0	27
CCCGTAG	25	3.8885177E-5	45.0	22
CTACGAA	100	0.0	45.0	11
GTCCGAT	40	6.8048394E-9	45.0	16
>>END_MODULE