FastQCFastQC Report
Sat 18 Jun 2016
SRR3552688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873765
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26420.30236963027816405No Hit
GCCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTC24060.2753600796552849No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC21860.2501816850068382No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC16750.19169914107340075No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG16410.1878079346277317No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16270.18620567315010328No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC16080.1840311754304647No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC16020.18334449194005253No Hit
GTATTCGGGCACTAACATTATATTGCAATGAGAGAAATTTTAAAAAGTCTC15040.17212866159665358No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT12280.1405412210376932No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT10890.12463305350981098No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9180.10506257403306381No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA207.0323556E-445.00000438
TCGATAT207.0323556E-445.00000419
ATCGTTC207.0323556E-445.00000434
TCCGTAC207.0323556E-445.00000432
TTACGCG207.0323556E-445.0000041
GCAATTC302.1649012E-645.0000049
CCGAACC207.0323556E-445.00000426
CGTCAAT302.1649012E-645.00000414
GTATTCG1650.045.01
CGAAACG253.8900354E-545.02
CGCGTAA253.8900354E-545.031
GCCCGTA253.8900354E-545.041
TTAACGG603.6379788E-1241.2500042
CGAATAT3450.040.43478414
TAGGGAC4700.040.2127655
TAGCCGT451.9274012E-840.044
CTACGCA1350.040.015
AACGCAT1800.040.027
GCTACGA3600.040.010
CCTGGGA19750.039.5316474