##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	873765
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65628286781915	31.0	31.0	34.0	30.0	34.0
2	31.756087735260625	31.0	31.0	34.0	30.0	34.0
3	31.81572848534789	33.0	31.0	34.0	30.0	34.0
4	35.51538914925638	37.0	35.0	37.0	33.0	37.0
5	35.411373767546195	37.0	35.0	37.0	33.0	37.0
6	35.3541558657076	37.0	35.0	37.0	33.0	37.0
7	35.7212030694752	37.0	35.0	37.0	35.0	37.0
8	35.49080931371707	37.0	35.0	37.0	33.0	37.0
9	37.27449829187482	39.0	37.0	39.0	34.0	39.0
10	36.81048909031605	39.0	37.0	39.0	32.0	39.0
11	36.72228574044509	39.0	35.0	39.0	32.0	39.0
12	36.66101755048554	39.0	35.0	39.0	32.0	39.0
13	36.46759025596127	39.0	35.0	39.0	32.0	39.0
14	37.55957265397447	39.0	36.0	41.0	32.0	41.0
15	37.72772198474418	40.0	36.0	41.0	33.0	41.0
16	37.790025922301766	40.0	36.0	41.0	33.0	41.0
17	37.703617105285744	39.0	36.0	41.0	33.0	41.0
18	37.70762275897982	39.0	36.0	41.0	33.0	41.0
19	37.62519670620819	39.0	36.0	41.0	33.0	41.0
20	37.50041944916539	39.0	36.0	41.0	32.0	41.0
21	37.34720548431214	39.0	35.0	41.0	32.0	41.0
22	37.41140352383078	39.0	35.0	41.0	32.0	41.0
23	33.2873335507831	39.0	34.0	40.0	0.0	41.0
24	35.00387060594095	39.0	34.0	41.0	18.0	41.0
25	36.038021664864125	38.0	34.0	40.0	27.0	41.0
26	36.64512883898989	38.0	35.0	40.0	30.0	41.0
27	30.346810641305158	38.0	31.0	40.0	0.0	41.0
28	32.908008446206935	38.0	30.0	40.0	16.0	41.0
29	34.88201289820489	38.0	31.0	40.0	25.0	41.0
30	35.9351118435735	38.0	34.0	40.0	30.0	41.0
31	36.32058848775128	38.0	35.0	40.0	30.0	41.0
32	34.80490292012154	38.0	35.0	40.0	25.0	41.0
33	35.49753537850566	38.0	35.0	40.0	25.0	41.0
34	35.91711959165222	38.0	35.0	40.0	28.0	41.0
35	36.126250193129735	38.0	35.0	40.0	30.0	41.0
36	36.16036577340589	38.0	35.0	40.0	30.0	41.0
37	36.151848609179815	38.0	35.0	40.0	30.0	41.0
38	36.113790321196205	38.0	35.0	40.0	30.0	41.0
39	36.093313419512114	38.0	35.0	40.0	30.0	41.0
40	35.99875481393739	38.0	35.0	40.0	30.0	41.0
41	35.894583783969374	38.0	35.0	40.0	29.0	41.0
42	35.85926650758499	38.0	35.0	40.0	29.0	41.0
43	35.82364823493731	38.0	35.0	40.0	29.0	41.0
44	35.75831831213198	38.0	35.0	40.0	29.0	41.0
45	35.698327353464606	38.0	35.0	40.0	29.0	41.0
46	35.55881043530011	38.0	34.0	40.0	29.0	41.0
47	35.453428553444006	38.0	34.0	40.0	28.0	41.0
48	35.41675049927612	37.0	34.0	40.0	28.0	41.0
49	35.41341779540265	37.0	34.0	40.0	28.0	41.0
50	35.33566691272825	37.0	34.0	40.0	28.0	41.0
51	34.59808300858926	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	39.0
10	77.0
11	57.0
12	56.0
13	57.0
14	49.0
15	76.0
16	126.0
17	221.0
18	382.0
19	776.0
20	1497.0
21	2628.0
22	3677.0
23	4696.0
24	5881.0
25	7262.0
26	9001.0
27	11277.0
28	13812.0
29	17423.0
30	22732.0
31	29034.0
32	37694.0
33	50205.0
34	73312.0
35	84677.0
36	81777.0
37	104200.0
38	140173.0
39	170793.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53249443500255	17.581042957774688	21.012057017619153	15.874405589603613
2	28.14612624675971	26.09568934438894	25.63481027507396	20.12337413377739
3	30.85062917374809	25.46394053320973	26.07714889014781	17.60828140289437
4	27.51506411907092	26.078522257128633	25.885907538068015	20.520506085732436
5	24.34510423283148	29.26107134069229	24.306535510119996	22.08728891635623
6	23.635073503745286	35.68110418705258	25.69718402545307	14.986638283749063
7	80.0622593031307	6.440518903824255	9.113663284750476	4.3835585082945645
8	80.91454796198062	6.505410493668206	7.489313488180461	5.090728056170709
9	75.73329213232391	7.386196517370232	10.20514669276064	6.675364657545221
10	38.797388313791465	27.130521364440096	17.386482635491234	16.685607686277205
11	29.521209936310104	28.252905529518806	22.819179069887213	19.406705464283874
12	25.529175464798886	22.149204877741727	30.87763872437097	21.443980933088415
13	24.67929019816541	24.92397841524895	30.651262067031755	19.745469319553884
14	19.952733286409963	30.410007267400275	28.139602753600794	21.49765669258897
15	19.113720508374676	25.80155991599572	34.03821393624144	21.046505639388165
16	21.084845467602843	25.448604601923858	30.795236705521507	22.67131322495179
17	20.842560642735748	24.097669281786295	28.069217695833547	26.990552379644413
18	23.665974260813837	24.174463385464055	28.51132741641059	23.648234937311518
19	23.443259915423482	26.119837713801765	27.60215847510486	22.834743895669888
20	24.603182777978063	25.786109537461442	26.53699793422717	23.073709750333325
21	24.641064817199133	26.699398579709648	28.278999502154466	20.380537100936753
22	21.238204780461565	25.704966438344407	26.838451986518113	26.218376794675912
23	18.15717040623051	35.33535904963005	24.359410138881735	22.14806040525771
24	21.125474240785564	25.58960361195516	28.693756330363428	24.591165816895845
25	21.398087586479203	27.98418339027084	27.466538485748455	23.151190537501503
26	19.899973104896628	27.095729400925876	27.76867922152982	25.23561827264768
27	17.110321425097137	39.910273357252805	23.339799602868048	19.639605614782006
28	19.314117640326632	27.004171602204252	29.87199075266233	23.809720004806785
29	21.150080399192003	26.618141033344205	27.627107975256504	24.604670592207288
30	21.19723266553364	26.437886617111005	28.178629265305887	24.186251452049465
31	22.890365258393274	28.414505044262473	24.31729355146979	24.37783614587446
32	21.432993997241823	30.91254513513359	23.600167092982666	24.054293774641923
33	21.29828958587263	25.837610799242363	25.0281254112948	27.835974203590208
34	19.72441102584791	27.36536711816106	26.0428147156272	26.86740714036383
35	20.381452678923967	25.066694134006283	26.717824586702378	27.83402860036738
36	21.26658769806527	27.40462252436296	26.847607766390276	24.481182011181495
37	20.483997413492187	27.207201020869455	27.520557586994215	24.788243978644143
38	20.13974009029888	28.226697109634742	25.23893724285134	26.39462555721504
39	21.289477147745675	25.40728914525073	26.56492306283726	26.738310644166337
40	22.031438659136036	26.024045366889265	27.459671650844335	24.48484432313036
41	19.01678368897816	25.608372960693092	27.66567669796799	27.70916665236076
42	20.15313041836192	26.003559309425306	27.97960550033476	25.86370477187802
43	21.513450412868448	23.44188654844266	29.158526606124074	25.88613643256482
44	20.967537037990763	24.36684921002787	27.598953952149607	27.06665979983176
45	21.718539882004887	24.498349098441803	26.492363507350376	27.29074751220294
46	21.703776186961026	26.4658117457211	26.100038339828213	25.73037372748966
47	20.329951417143054	26.545924819602522	28.570153302089235	24.553970461165186
48	20.010300252356185	25.371924945494502	28.579309081961394	26.03846572018792
49	20.596155716926177	24.464014923921194	30.03759592110007	24.902233438052566
50	20.3495218966198	24.007484850045493	29.403329270456013	26.239663982878692
51	19.564986008823883	24.304933248642367	27.041023616189708	29.08905712634404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1927.0
1	2440.0
2	2953.0
3	4365.5
4	5778.0
5	4052.5
6	2327.0
7	2176.0
8	2025.0
9	2080.0
10	2135.0
11	2280.0
12	2425.0
13	2287.0
14	2149.0
15	2210.0
16	2271.0
17	2222.5
18	2174.0
19	2331.0
20	2488.0
21	2740.5
22	2993.0
23	3077.0
24	3161.0
25	4219.0
26	6425.0
27	7573.0
28	8635.5
29	9698.0
30	12591.5
31	15485.0
32	16095.5
33	16706.0
34	18277.0
35	19848.0
36	21424.5
37	23001.0
38	24905.5
39	26810.0
40	30743.0
41	34676.0
42	40274.0
43	45872.0
44	51853.5
45	57835.0
46	65942.5
47	74050.0
48	78299.5
49	82549.0
50	84035.5
51	85522.0
52	75338.5
53	65155.0
54	58957.0
55	52759.0
56	47878.0
57	42997.0
58	40403.0
59	37809.0
60	35812.0
61	33815.0
62	30194.5
63	26574.0
64	24613.5
65	22653.0
66	18944.5
67	15236.0
68	14004.5
69	12773.0
70	10725.0
71	8677.0
72	7390.5
73	6104.0
74	4997.0
75	3189.0
76	2488.0
77	2087.0
78	1686.0
79	1181.5
80	677.0
81	560.5
82	444.0
83	305.0
84	166.0
85	131.0
86	96.0
87	64.5
88	33.0
89	21.5
90	10.0
91	6.5
92	3.0
93	7.0
94	11.0
95	6.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	873765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.506582297184487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.16156953655934	21.587478725216734
2	10.636854847429385	6.277144658777617
3	4.346850806911524	3.84782133203153
4	2.3504992118343813	2.7742079373383377
5	1.408989098693101	2.0787226398211884
6	0.979424528006851	1.7339682263709133
7	0.6828343084796901	1.4103674702947921
8	0.5108538860399535	1.2058841784219514
9	0.40636057767780814	1.0791280644823507
>10	4.49142210393169	32.3481531822983
>50	0.9298842252476196	18.051621206395538
>100	0.08532868435111647	4.3419026836067784
>500	0.0047625312195971985	1.0142465448315212
>1k	0.004365653617964098	2.2493531501124417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2642	0.30236963027816405	No Hit
GCCCCTGGGAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTC	2406	0.2753600796552849	No Hit
GCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	2186	0.2501816850068382	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGC	1675	0.19169914107340075	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCG	1641	0.1878079346277317	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1627	0.18620567315010328	No Hit
CCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGC	1608	0.1840311754304647	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTC	1602	0.18334449194005253	No Hit
GTATTCGGGCACTAACATTATATTGCAATGAGAGAAATTTTAAAAAGTCTC	1504	0.17212866159665358	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1228	0.1405412210376932	No Hit
CTGTCTCTTATACACATCTGACGCATCCCACTTCGTATGCCGTCTTCTGCT	1089	0.12463305350981098	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	918	0.10506257403306381	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1444724840203029E-4	0.0	0.0	0.15244373487150434	0.0
2	1.1444724840203029E-4	0.0	0.0	0.8489696886462608	0.0
3	1.1444724840203029E-4	0.0	0.0	1.1015547658695415	0.0
4	1.1444724840203029E-4	0.0	0.0	1.476483951634593	0.0
5	1.1444724840203029E-4	0.0	0.0	2.6004703781909324	0.0
6	1.1444724840203029E-4	0.0	0.0	3.352274352943869	0.0
7	1.1444724840203029E-4	0.0	0.0	4.076153199086711	0.0
8	1.1444724840203029E-4	0.0	0.0	5.1826291966375395	0.0
9	1.1444724840203029E-4	0.0	0.0	5.620962158017316	0.0
10	1.1444724840203029E-4	0.0	0.0	6.858365807740068	0.0
11	1.1444724840203029E-4	0.0	0.0	8.108015313041836	0.0
12	1.1444724840203029E-4	0.0	0.0	9.435031158263378	0.0
13	1.1444724840203029E-4	0.0	0.0	9.947754831104474	0.0
14	1.1444724840203029E-4	0.0	0.0	10.123889146395198	0.0
15	1.1444724840203029E-4	0.0	0.0	10.409892820151871	0.0
16	1.1444724840203029E-4	0.0	0.0	11.057320904362157	0.0
17	1.1444724840203029E-4	0.0	0.0	11.68494961459889	0.0
18	1.1444724840203029E-4	0.0	0.0	12.815001745320538	0.0
19	1.1444724840203029E-4	0.0	0.0	13.22552402533862	0.0
20	1.1444724840203029E-4	0.0	0.0	13.641310878783196	0.0
21	1.1444724840203029E-4	0.0	0.0	14.071746980023233	0.0
22	1.1444724840203029E-4	0.0	0.0	14.522211349733624	0.0
23	1.1444724840203029E-4	0.0	0.0	14.98194594656458	0.0
24	1.1444724840203029E-4	0.0	0.0	15.34440038225381	0.0
25	1.1444724840203029E-4	0.0	0.0	15.680760845307377	0.0
26	1.1444724840203029E-4	0.0	0.0	15.970197936516112	0.0
27	1.1444724840203029E-4	0.0	0.0	16.357716319605387	0.0
28	1.1444724840203029E-4	0.0	0.0	16.74454801920425	0.0
29	1.1444724840203029E-4	0.0	0.0	17.13515647800038	0.0
30	1.1444724840203029E-4	0.0	0.0	17.58974094865324	0.0
31	1.1444724840203029E-4	0.0	0.0	17.956201038036543	0.0
32	1.1444724840203029E-4	0.0	0.0	18.341316028909375	0.0
33	1.1444724840203029E-4	0.0	0.0	18.73352674918313	0.0
34	1.1444724840203029E-4	0.0	0.0	19.125508574960087	0.0
35	1.1444724840203029E-4	0.0	0.0	19.581351965345373	0.0
36	1.1444724840203029E-4	0.0	0.0	19.97310489662552	0.0
37	1.1444724840203029E-4	0.0	0.0	20.363370013676445	0.0
38	1.1444724840203029E-4	0.0	0.0	20.729830103059747	0.0
39	1.1444724840203029E-4	0.0	0.0	21.100753635130726	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.0323556E-4	45.000004	38
TCGATAT	20	7.0323556E-4	45.000004	19
ATCGTTC	20	7.0323556E-4	45.000004	34
TCCGTAC	20	7.0323556E-4	45.000004	32
TTACGCG	20	7.0323556E-4	45.000004	1
GCAATTC	30	2.1649012E-6	45.000004	9
CCGAACC	20	7.0323556E-4	45.000004	26
CGTCAAT	30	2.1649012E-6	45.000004	14
GTATTCG	165	0.0	45.0	1
CGAAACG	25	3.8900354E-5	45.0	2
CGCGTAA	25	3.8900354E-5	45.0	31
GCCCGTA	25	3.8900354E-5	45.0	41
TTAACGG	60	3.6379788E-12	41.250004	2
CGAATAT	345	0.0	40.434784	14
TAGGGAC	470	0.0	40.212765	5
TAGCCGT	45	1.9274012E-8	40.0	44
CTACGCA	135	0.0	40.0	15
AACGCAT	180	0.0	40.0	27
GCTACGA	360	0.0	40.0	10
CCTGGGA	1975	0.0	39.531647	4
>>END_MODULE