FastQCFastQC Report
Sat 18 Jun 2016
SRR3552682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757389
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30340.4005867526462623No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC19940.2632729020358099TruSeq Adapter, Index 16 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19600.25878379538123736No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG17070.22537956056927155No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC16170.21349663118952084No Hit
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC15920.21019581747292343TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC15900.20993175237559564No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT12820.16926572738711546TruSeq Adapter, Index 13 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11500.15183743096348112No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT9820.1296559627879465No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGG351.2111923E-745.0000042
TCACGGA207.031761E-445.017
GCCCCGT207.031761E-445.011
CGTTGCC253.8895418E-545.024
TCGCTAA207.031761E-445.014
GCCTATT207.031761E-445.027
ATAACGG850.045.02
GCTACGA2000.045.010
CGTTTAC207.031761E-445.033
TATAACG207.031761E-445.01
CTAGGCG207.031761E-445.01
CGATAGT253.8895418E-545.010
ATCGCCT253.8895418E-545.039
TCGAAAC253.8895418E-545.027
TACGTTG253.8895418E-545.01
CGCCAAT253.8895418E-545.014
GATAACG551.8189894E-1244.9999961
TAGAACG302.1645174E-644.9999961
TTGCGAG302.1645174E-644.9999961
AGTACGT302.1645174E-644.99999633
Produced by FastQC (version 0.11.5)