Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552679_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 458466 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 45 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1324 | 0.2887891359446502 | No Hit |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 1229 | 0.26806786108457337 | No Hit |
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC | 835 | 0.18212910008593877 | TruSeq Adapter, Index 22 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC | 833 | 0.18169286272046345 | TruSeq Adapter, Index 22 (95% over 22bp) |
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 792 | 0.17274999672821978 | No Hit |
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT | 676 | 0.1474482295306522 | TruSeq Adapter, Index 20 (95% over 22bp) |
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC | 485 | 0.10578756112776085 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAAAACG | 35 | 1.2097917E-7 | 45.000004 | 1 |
TATTTCG | 25 | 3.8871243E-5 | 45.0 | 28 |
TCGTTCG | 20 | 7.0288463E-4 | 45.0 | 27 |
CTCCGTA | 20 | 7.0288463E-4 | 45.0 | 14 |
CGAGTTA | 25 | 3.8871243E-5 | 45.0 | 11 |
GCGAAAT | 20 | 7.0288463E-4 | 45.0 | 7 |
TTCACCG | 50 | 2.1827873E-11 | 45.0 | 23 |
CGATTAC | 20 | 7.0288463E-4 | 45.0 | 10 |
TACGGGC | 80 | 0.0 | 45.0 | 4 |
TAACGCA | 85 | 0.0 | 45.0 | 26 |
TGCGGAT | 20 | 7.0288463E-4 | 45.0 | 4 |
CGTTCGA | 20 | 7.0288463E-4 | 45.0 | 28 |
TCGATAC | 20 | 7.0288463E-4 | 45.0 | 11 |
AACGGAC | 20 | 7.0288463E-4 | 45.0 | 41 |
GCCGATA | 20 | 7.0288463E-4 | 45.0 | 9 |
TAACCGG | 20 | 7.0288463E-4 | 45.0 | 26 |
CGCTCGT | 50 | 2.1827873E-11 | 45.0 | 13 |
ACTGTCG | 20 | 7.0288463E-4 | 45.0 | 15 |
CTCGAGG | 45 | 3.8380676E-10 | 45.0 | 2 |
TATAGCG | 25 | 3.8871243E-5 | 45.0 | 1 |