FastQCFastQC Report
Sat 18 Jun 2016
SRR3552679_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552679_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458466
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13240.2887891359446502No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12290.26806786108457337No Hit
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC8350.18212910008593877TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC8330.18169286272046345TruSeq Adapter, Index 22 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7920.17274999672821978No Hit
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT6760.1474482295306522TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC4850.10578756112776085No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAAACG351.2097917E-745.0000041
TATTTCG253.8871243E-545.028
TCGTTCG207.0288463E-445.027
CTCCGTA207.0288463E-445.014
CGAGTTA253.8871243E-545.011
GCGAAAT207.0288463E-445.07
TTCACCG502.1827873E-1145.023
CGATTAC207.0288463E-445.010
TACGGGC800.045.04
TAACGCA850.045.026
TGCGGAT207.0288463E-445.04
CGTTCGA207.0288463E-445.028
TCGATAC207.0288463E-445.011
AACGGAC207.0288463E-445.041
GCCGATA207.0288463E-445.09
TAACCGG207.0288463E-445.026
CGCTCGT502.1827873E-1145.013
ACTGTCG207.0288463E-445.015
CTCGAGG453.8380676E-1045.02
TATAGCG253.8871243E-545.01